##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921682_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3593289
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.462933540831255	33.0	31.0	34.0	29.0	34.0
2	31.610849280422478	34.0	31.0	34.0	29.0	34.0
3	31.683484128329226	34.0	31.0	34.0	29.0	34.0
4	35.159852157730704	37.0	35.0	37.0	33.0	37.0
5	34.91581306151551	37.0	35.0	37.0	32.0	37.0
6	34.88984604355508	37.0	35.0	37.0	32.0	37.0
7	34.78345437842601	37.0	35.0	37.0	32.0	37.0
8	34.73002672481952	37.0	35.0	37.0	32.0	37.0
9	36.32969989332892	39.0	37.0	39.0	32.0	39.0
10-11	36.232215249037864	39.0	37.0	39.0	32.0	39.0
12-13	36.16235376558913	39.0	37.0	39.0	32.0	39.0
14-15	37.417027686890755	40.0	38.0	41.0	32.0	41.0
16-17	37.38502845164973	40.0	38.0	41.0	32.0	41.0
18-19	37.35187289416465	40.0	38.0	41.0	32.0	41.0
20-21	37.29649702542712	40.0	38.0	41.0	31.0	41.0
22-23	37.21127969389604	40.0	38.0	41.0	31.0	41.0
24-25	37.13294018377036	40.0	38.0	41.0	31.0	41.0
26-27	37.0124205150212	40.0	37.0	41.0	31.0	41.0
28-29	36.89684269759543	40.0	37.0	41.0	30.0	41.0
30-31	36.74897412927265	40.0	37.0	41.0	30.0	41.0
32-33	36.700506277118265	40.0	37.0	41.0	30.0	41.0
34-35	36.82985573940755	40.0	37.0	41.0	30.0	41.0
36-37	36.82857460115231	40.0	37.0	41.0	30.0	41.0
38-39	36.73095442643216	40.0	37.0	41.0	30.0	41.0
40-41	36.65862486986157	40.0	37.0	41.0	30.0	41.0
42-43	36.5463578075685	40.0	37.0	41.0	30.0	41.0
44-45	36.427936077504484	40.0	37.0	41.0	30.0	41.0
46-47	36.28176901440435	40.0	36.0	41.0	29.0	41.0
48-49	36.15032773595445	40.0	36.0	41.0	28.5	41.0
50-51	36.00694294280254	40.0	36.0	41.0	28.0	41.0
52-53	35.84383833307034	39.5	35.0	41.0	28.0	41.0
54-55	35.640677245832435	39.0	35.0	41.0	28.0	41.0
56-57	35.422984764097734	39.0	35.0	41.0	27.0	41.0
58-59	35.190808894024386	39.0	35.0	41.0	27.0	41.0
60-61	34.921887997319445	38.0	35.0	40.0	26.0	41.0
62-63	34.62918178860648	38.0	34.5	40.0	26.0	41.0
64-65	34.29616891377231	37.0	34.0	40.0	26.0	41.0
66-67	33.94854602566062	37.0	34.0	39.5	26.0	41.0
68-69	33.58566956345565	36.0	34.0	39.0	25.0	41.0
70-71	33.18323087845147	36.0	33.5	39.0	24.0	40.0
72-73	32.80965780932176	35.0	33.0	38.0	23.5	40.0
74-75	32.39176879455006	35.0	33.0	37.0	22.5	39.0
76-77	29.65886058705548	33.0	29.0	34.5	18.5	36.5
78-79	31.224738672564328	34.5	31.5	36.0	20.0	37.5
80-81	31.389566077206702	35.0	32.0	36.0	20.0	37.0
82-83	31.202821008830625	35.0	32.0	35.0	20.0	37.0
84-85	30.995422021440525	35.0	32.0	35.0	19.5	36.5
86-87	30.782119528932967	35.0	32.0	35.0	18.5	36.0
88-89	30.506141309535636	35.0	32.0	35.0	14.5	36.0
90-91	30.311839097829314	34.5	32.0	35.0	9.0	35.0
92-93	30.150986603081467	34.0	32.0	35.0	7.0	35.0
94-95	30.047190331754557	34.0	31.5	35.0	2.0	35.0
96-97	29.847950304025087	34.0	31.0	35.0	2.0	35.0
98-99	29.649739277859364	34.0	31.0	35.0	2.0	35.0
100-101	28.544072992737295	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	60401.0
3	20558.0
4	11090.0
5	8096.0
6	7010.0
7	7039.0
8	7436.0
9	8086.0
10	9109.0
11	10008.0
12	10583.0
13	11353.0
14	12192.0
15	12877.0
16	13713.0
17	14856.0
18	14222.0
19	13034.0
20	12640.0
21	12685.0
22	13472.0
23	14968.0
24	17804.0
25	21311.0
26	25774.0
27	31400.0
28	37796.0
29	45670.0
30	55503.0
31	69588.0
32	88284.0
33	116512.0
34	164778.0
35	244452.0
36	428768.0
37	850823.0
38	970657.0
39	118736.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.256766800124666	19.798552944318406	14.190389797348644	25.754290458208278
2	14.864810333719703	21.178809919057354	37.98257026648318	25.973809480739767
3	17.38509650068134	25.23490590404261	29.287561565386227	28.09243602988983
4	12.835907131201765	17.609681305869717	36.267324674254795	33.28708688867372
5	14.215736080880918	37.42546299170208	34.49748534591491	13.861315581502101
6	32.81655451099571	36.15253171250299	17.51301061443496	13.517903162066336
7	28.407678870249516	31.875198460240746	21.571546290877244	18.145576378632498
8	26.33865519862165	34.84974350796721	20.228125263512066	18.58347602989907
9	26.441146070755288	15.781265393291822	19.49341076663533	38.28417776931756
10-11	25.18762113964615	25.63539754408641	28.255257749526443	20.921723566741
12-13	25.934091378401558	22.980924381581385	27.846579084612994	23.238405155404063
14-15	23.216229669183853	24.524306806506974	25.16375490401947	27.095708620289706
16-17	23.504385536482037	27.411363238526043	25.861265264218936	23.222985960772984
18-19	23.456072211192243	26.990293991102178	26.95904084282755	22.594592954878024
20-21	24.158083620362536	26.116811805830086	26.72699324056839	22.99811133323899
22-23	23.861843564489245	25.986498720253227	26.424815816373243	23.72684189888428
24-25	23.736989390859772	26.091349396420167	26.427923093439	23.743738119281062
26-27	23.60355456182265	26.3344183037586	26.415070972539546	23.646956161879203
28-29	23.700209039722555	26.515064859089527	26.220082563871777	23.564643537316137
30-31	24.075641058471568	26.1982234850467	26.11016875555996	23.61596670092177
32-33	23.80191675505999	26.188327739341798	26.01562328217515	23.99413222342307
34-35	24.095775067337712	26.286987034837484	26.070434795314924	23.546803102509884
36-37	24.127034752737657	26.13244675181256	26.06889486511331	23.671623630336473
38-39	23.98759186917612	26.21365829467098	26.060943052451393	23.737806783701508
40-41	24.2053994887806	26.022929313043193	26.05707739968582	23.714593798490387
42-43	24.004942411361256	26.007498504724346	25.977272923362012	24.01028616055239
44-45	23.98884511605059	26.019184285195422	26.05170544760781	23.94026515114617
46-47	24.076841415503566	25.978423209723232	25.882755502702143	24.061979872071053
48-49	23.83815114489561	26.03125353555047	26.017657539258266	24.112937780295656
50-51	23.961696463284884	26.044941087216856	26.002040524959273	23.991321924538983
52-53	24.09990589224739	25.93328855457422	25.983368962920878	23.98343659025752
54-55	23.750567746646954	25.848669914325917	26.170476506421103	24.230285832606025
56-57	23.823602674626535	25.908085805136338	26.0620368630889	24.206274657148224
58-59	23.86753576310892	25.86684075686931	26.138608515120698	24.127014964901072
60-61	23.920432169461193	25.877273669752732	26.176783694007987	24.025510466778087
62-63	23.87089474154481	25.878114112037746	26.154936442205372	24.09605470421207
64-65	23.711916686456956	25.73976934870786	26.099864442321724	24.44844952251346
66-67	23.730292492925244	25.896863437166058	25.95323872476844	24.419605345140262
68-69	23.784860740806536	25.850655605583317	25.981591560290195	24.382892093319956
70-71	23.722432186234535	25.64201784157243	26.016625139556986	24.618924832636047
72-73	23.694873981664923	25.81907707143765	25.882202119025028	24.603846827872403
74-75	23.705733751818112	25.880940616453135	25.802913155129335	24.610412476599418
76-77	23.657990022400714	25.624597185356873	26.005999279483937	24.711413512758476
78-79	23.56652911800945	25.737819325714007	26.019132767995305	24.67651878828124
80-81	23.669870476093404	25.822949044684435	25.837471941081873	24.669708538140288
82-83	23.639772952976728	25.88299041657347	25.844101633621175	24.63313499682863
84-85	23.612423265095757	25.81851260628447	25.88308311218144	24.68598101643833
86-87	23.465658327707935	25.95233559908835	25.833283206009934	24.748722867193784
88-89	23.425380531003093	25.76835401099341	25.85421126186992	24.952054196133577
90-91	23.342920617676423	25.801271896928508	25.919010150109294	24.936797335285775
92-93	23.396586088737635	25.663365917579846	25.979977757395222	24.9600702362873
94-95	23.268331879537776	25.703805901315206	25.951851070474106	25.07601114867291
96-97	23.16898052661259	25.644752216857636	26.009380693840672	25.1768865626891
98-99	23.024905614589148	25.598957043297343	25.91709557319482	25.459041768918688
100-101	23.008761635264058	25.632312436635175	25.941111168935116	25.41781475916565
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	37.0
2	45.5
3	56.5
4	56.5
5	65.5
6	108.0
7	260.5
8	399.5
9	409.5
10	337.5
11	281.5
12	294.0
13	309.5
14	350.0
15	407.0
16	447.5
17	467.5
18	512.0
19	610.0
20	712.0
21	842.5
22	988.5
23	1202.5
24	1511.5
25	1909.0
26	2469.0
27	3299.5
28	4536.0
29	6509.5
30	9920.0
31	14848.5
32	21962.0
33	29948.5
34	38358.0
35	48020.0
36	60120.5
37	72816.0
38	84661.0
39	95811.5
40	105773.5
41	115798.5
42	125577.5
43	135354.0
44	144511.5
45	154051.5
46	160699.5
47	164904.5
48	169066.0
49	169262.0
50	166138.5
51	161929.0
52	157612.5
53	154128.5
54	148855.5
55	138996.0
56	126319.5
57	111770.0
58	98939.0
59	85878.5
60	69666.0
61	56295.5
62	45358.0
63	33739.5
64	23831.5
65	17075.5
66	12616.0
67	9714.5
68	7194.5
69	5009.5
70	3546.5
71	2527.5
72	1711.0
73	1185.5
74	856.0
75	572.5
76	341.5
77	204.0
78	128.0
79	68.5
80	45.5
81	33.5
82	20.0
83	13.0
84	10.5
85	9.5
86	8.5
87	6.0
88	3.5
89	3.5
90	3.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1709575823152549
2	1.1131862758603608E-4
3	0.010046506139639757
4	0.0061225245172319845
5	0.02632685542409753
6	0.0019202463258591225
7	0.0
8	0.0
9	8.348897068952706E-5
10-11	0.05220843633785092
12-13	3.061262258615992E-4
14-15	0.0053015496387849685
16-17	0.0
18-19	0.00141931250172196
20-21	3.757003681028718E-4
22-23	0.0
24-25	5.426783094819259E-4
26-27	0.002880369488788684
28-29	0.005343294124129732
30-31	0.017727491443076244
32-33	0.012412026975843022
34-35	0.010895310674983281
36-37	0.004508404417234461
38-39	0.0
40-41	0.003214325371546792
42-43	0.00851587501033176
44-45	0.006790436282748201
46-47	0.0034091329698223553
48-49	0.008891575378434631
50-51	0.002671647062064866
52-53	0.001878501840514359
54-55	0.0038126629948217355
56-57	0.007347029420678382
58-59	0.0143461881301504
60-61	0.007068732851713291
62-63	0.020371308848244604
64-65	0.04063129906890317
66-67	0.06057124823525188
68-69	0.05116482420423183
70-71	0.0685166152792052
72-73	0.03374345898701719
74-75	0.026827789248234694
76-77	0.23261418716947063
78-79	0.0880947789058993
80-81	0.06699989897834546
82-83	0.02764876412668171
84-85	0.05193013976888583
86-87	0.06770955522920645
88-89	0.29493870378920256
90-91	0.2996140861478161
92-93	0.05518620962577739
94-95	0.010992714474121063
96-97	0.01644732722583683
98-99	0.0011966752465498878
100-101	2.782965689650902E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3593289.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.00429098949197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.77534798759915	48.84409599421588
2	12.116027115184076	15.02491341772897
3	3.872567527029363	7.203474114671579
4	1.7269457344222277	4.283121833607109
5	0.9376969218957306	2.90706164025869
6	0.5598016079465375	2.082606107730157
7	0.3959310797302092	1.718459811556295
8	0.2790703835322516	1.3842849013666285
9	0.20981182780516655	1.1708310261841854
>10	1.0662810304390786	11.59410677188514
>50	0.04726256623651987	1.9507379519604784
>100	0.01204172740703597	1.2271836673238563
>500	9.895850839564634E-4	0.4066396514878883
>1k	2.249056887459612E-4	0.20248311002325592
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.782965689650902E-5	0.0
3	0.0	0.0	0.0	2.782965689650902E-5	0.0
4	0.0	0.0	0.0	2.782965689650902E-5	0.0
5	0.0	0.0	0.0	2.782965689650902E-5	0.0
6	0.0	0.0	0.0	2.782965689650902E-5	0.0
7	0.0	0.0	0.0	2.782965689650902E-5	0.0
8	0.0	0.0	0.0	2.782965689650902E-5	0.0
9	0.0	0.0	0.0	8.348897068952706E-5	0.0
10-11	0.0	0.0	0.0	8.348897068952706E-5	0.0
12-13	0.0	0.0	0.0	8.348897068952706E-5	6.957414224127255E-5
14-15	0.0	0.0	0.0	8.348897068952706E-5	1.2523345603429058E-4
16-17	0.0	0.0	0.0	9.740379913778157E-5	1.6697794137905412E-4
18-19	0.0	0.0	0.0	1.1131862758603608E-4	1.9480759827556314E-4
20-21	0.0	0.0	0.0	1.391482844825451E-4	1.9480759827556314E-4
22-23	0.0	0.0	0.0	1.9480759827556312E-4	2.782965689650902E-4
24-25	0.0	0.0	0.0	3.896151965511263E-4	3.061262258615992E-4
26-27	0.0	0.0	0.0	6.122524517231984E-4	3.4787071120636275E-4
28-29	0.0	0.0	0.0	0.001474971815514978	3.6178553965461726E-4
30-31	0.0	0.0	0.0	0.007152221822402818	3.6178553965461726E-4
32-33	0.0	0.0	0.0	0.024490098068927938	3.896151965511263E-4
34-35	0.0	0.0	0.0	0.04893845165251111	4.174448534476353E-4
36-37	0.0	0.0	0.0	0.0874546967972796	4.4527451034414434E-4
38-39	0.0	0.0	0.0	0.15530340031096856	5.009338241371623E-4
40-41	0.0	0.0	0.0	0.23134793778067947	5.009338241371623E-4
42-43	0.0	0.0	0.0	0.31389070013572523	5.009338241371623E-4
44-45	0.0	0.0	0.0	0.419671225999356	5.009338241371623E-4
46-47	0.0	0.0	0.0	0.542845287423305	5.009338241371623E-4
48-49	0.0	0.0	0.0	0.6723645106196579	5.009338241371623E-4
50-51	0.0	0.0	0.0	0.8071713686263475	5.287634810336714E-4
52-53	0.0	0.0	0.0	0.9457908896278591	5.705079663784349E-4
54-55	0.0	0.0	0.0	1.1049069529336495	6.122524517231984E-4
56-57	0.0	0.0	0.0	1.289194940902332	6.400821086197075E-4
58-59	0.0	0.0	0.0	1.484475643345136	6.679117655162165E-4
60-61	0.0	0.0	0.0	1.6956470798758463	6.679117655162165E-4
62-63	0.0	0.0	0.0	1.9190913950979172	6.679117655162165E-4
64-65	0.0	0.0	0.0	2.1630044229673704	7.0965625086098E-4
66-67	0.0	0.0	0.0	2.439561638376429	7.37485907757489E-4
68-69	0.0	0.0	0.0	2.7442546369078578	7.514007362057436E-4
70-71	0.0	0.0	0.0	3.062166722465129	7.792303931022526E-4
72-73	0.0	0.0	0.0	3.3958721383111685	8.905490206882887E-4
74-75	0.0	5.565931379301804E-5	0.0	3.7500462668045906	9.183786775847977E-4
76-77	0.0	5.565931379301804E-5	0.0	4.150139329177252	9.601231629295612E-4
78-79	0.0	5.565931379301804E-5	0.0	4.597640212073117	0.0010018676482743247
80-81	0.0	5.565931379301804E-5	0.0	5.061755400136198	0.0010296973051708337
82-83	0.0	5.565931379301804E-5	0.0	5.546645427072523	0.0011131862758603608
84-85	0.0	5.565931379301804E-5	0.0	6.057778820462256	0.0011131862758603608
86-87	0.0	6.957414224127255E-5	0.0	6.605633446126932	0.0011131862758603608
88-89	0.0	8.348897068952706E-5	0.0	7.206044378840666	0.0011131862758603608
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4640	0.0	43.84545	1
GGTATCA	2575	0.0	41.16484	1
TCAACGC	5820	0.0	33.9209	4
CAACGCA	5895	0.0	33.277008	5
ATCAACG	6110	0.0	32.310905	3
TATCAAC	6240	0.0	31.86592	2
AACGCAG	6435	0.0	30.699978	6
ACGCAGA	7735	0.0	24.741495	7
GTACATG	9235	0.0	23.161913	1
CGCAGAG	8065	0.0	23.02256	8
TACATGG	9390	0.0	22.035244	2
GTATTAA	1900	0.0	20.01406	1
ACATGGG	9815	0.0	19.969019	3
GCAGAGT	9070	0.0	19.529396	9
TAAGGCG	725	0.0	18.999317	5
CATGGGG	8045	0.0	18.404564	4
AGAGTAC	7845	0.0	18.190254	10-11
ATGGGGG	4505	0.0	16.974972	5
GTATATA	1190	0.0	16.776491	1
TAAGGTG	2060	0.0	16.370771	5
>>END_MODULE