FastQCFastQC Report
Mon 15 Aug 2016
SRR2921681_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921681_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2769528
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21050.046.4443361
GTATCAA38350.043.931961
TCAACGC45350.036.0068474
CAACGCA46300.035.368655
TATCAAC48000.034.709962
ATCAACG49950.032.879183
AACGCAG50900.032.058746
GTACATG74500.028.984961
TACATGG77100.026.9039042
ACGCAGA59900.026.375087
CGCAGAG63050.024.9095768
ACATGGG82650.024.8671173
CATGGGG71800.022.6763734
GCAGAGT70150.020.2239959
GTATATA7400.019.8814281
TAAGGCG8000.019.5796245
GTATTAA13050.019.2744941
GTATAGC9400.019.1855091
ATGGGGG39700.019.0102145
GAGTACA55700.017.9781441
Produced by FastQC (version 0.11.5)