##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921681_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2769528 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.221737061333194 31.0 31.0 34.0 26.0 34.0 2 30.281147184646624 31.0 31.0 34.0 26.0 34.0 3 30.34451682741608 31.0 31.0 34.0 26.0 34.0 4 33.79686322001439 37.0 35.0 37.0 28.0 37.0 5 33.783247181469186 37.0 35.0 37.0 28.0 37.0 6 33.82217294788137 37.0 35.0 37.0 29.0 37.0 7 33.73306751186484 37.0 35.0 37.0 29.0 37.0 8 33.69998209081114 37.0 35.0 37.0 28.0 37.0 9 35.241514077489015 39.0 35.0 39.0 29.0 39.0 10-11 35.13522394429664 39.0 35.0 39.0 28.0 39.0 12-13 35.0252505120006 39.0 35.0 39.0 27.0 39.0 14-15 36.14500647763806 40.0 36.0 41.0 27.0 41.0 16-17 36.084465475705606 40.0 36.0 41.0 27.0 41.0 18-19 36.043774968153414 40.0 36.0 41.0 27.0 41.0 20-21 35.9655955816298 40.0 36.0 41.0 27.0 41.0 22-23 35.8664904272497 40.0 36.0 41.0 27.0 41.0 24-25 35.75639748000381 40.0 36.0 41.0 27.0 41.0 26-27 35.607671415490294 40.0 36.0 41.0 26.0 41.0 28-29 35.466164090054335 40.0 36.0 41.0 25.0 41.0 30-31 35.31268775762513 39.5 35.0 41.0 25.0 41.0 32-33 35.136375945648496 39.0 35.0 41.0 24.0 41.0 34-35 34.963224780540216 39.0 35.0 41.0 24.0 41.0 36-37 34.930847061304306 39.0 35.0 41.0 24.0 41.0 38-39 35.110817258392046 39.5 35.0 41.0 24.0 41.0 40-41 35.1716931549347 40.0 35.0 41.0 24.0 41.0 42-43 35.01322481664746 40.0 35.0 41.0 23.0 41.0 44-45 34.89087797631943 40.0 35.0 41.0 22.5 41.0 46-47 34.732258709787374 39.5 35.0 41.0 21.5 41.0 48-49 34.567025861446425 39.0 35.0 41.0 19.5 41.0 50-51 32.872746908498485 37.0 32.5 39.5 16.0 40.0 52-53 32.98928535837153 37.5 32.5 39.5 14.5 40.0 54-55 33.89910103815524 38.5 34.0 40.0 15.0 41.0 56-57 33.916758198508916 38.5 34.0 41.0 14.5 41.0 58-59 33.71954282462571 38.0 34.0 40.5 13.0 41.0 60-61 33.46516753035174 38.0 33.5 40.0 11.0 41.0 62-63 33.17156461317596 37.0 33.0 40.0 8.0 41.0 64-65 32.877240273432875 37.0 33.0 40.0 7.0 41.0 66-67 32.53484799575956 36.0 33.0 39.0 4.0 41.0 68-69 32.170315302824164 36.0 32.5 39.0 2.0 41.0 70-71 31.759970832575082 35.0 32.0 38.5 2.0 40.5 72-73 31.39645528046656 35.0 32.0 37.5 2.0 40.0 74-75 31.001038805168246 35.0 31.5 37.0 2.0 39.0 76-77 30.626651544956395 35.0 31.0 36.5 2.0 39.0 78-79 30.283810634880744 35.0 31.0 36.0 2.0 38.0 80-81 29.96906747286902 35.0 31.0 35.5 2.0 37.0 82-83 29.66507451811283 35.0 31.0 35.0 2.0 37.0 84-85 29.409278404117956 35.0 30.5 35.0 2.0 36.0 86-87 29.174115950443543 35.0 30.0 35.0 2.0 36.0 88-89 28.953443691488225 34.0 30.0 35.0 2.0 36.0 90-91 28.729843857870364 34.0 30.0 35.0 2.0 35.0 92-93 28.540026134417126 34.0 30.0 35.0 2.0 35.0 94-95 28.308175797464408 34.0 29.5 35.0 2.0 35.0 96-97 28.068823821243186 34.0 29.0 35.0 2.0 35.0 98-99 27.800534242657953 34.0 29.0 35.0 2.0 35.0 100-101 26.72453555262846 33.0 26.5 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 103856.0 3 17476.0 4 11664.0 5 9072.0 6 8161.0 7 8306.0 8 8679.0 9 9421.0 10 10341.0 11 11067.0 12 10906.0 13 11181.0 14 11754.0 15 12267.0 16 12276.0 17 12753.0 18 12406.0 19 11927.0 20 12136.0 21 12665.0 22 14020.0 23 15516.0 24 18723.0 25 21956.0 26 26207.0 27 30511.0 28 36287.0 29 42724.0 30 50466.0 31 61149.0 32 76490.0 33 98130.0 34 136359.0 35 197294.0 36 328320.0 37 586698.0 38 609744.0 39 100613.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.42592319630981 17.508287596206927 12.693822814516805 25.371966392966456 2 15.95276002546793 19.41416534352436 39.46304150969031 25.170033121317392 3 19.302520786743603 23.504891944344173 29.094394995644095 28.098192273268126 4 13.52561434241297 15.396139203732451 36.0930684311396 34.98517802271498 5 14.533234042568566 36.925108097942314 34.07638812861117 14.465269730877948 6 34.055839130725246 34.58252458738158 17.081545064377682 14.280091217515498 7 29.339433459618803 31.542766428215717 20.783403265221516 18.33439684694396 8 26.700620758221373 34.70420901448267 19.82854664426173 18.76662358303422 9 27.1572787473771 15.57206239571135 19.094901812947914 38.175757043963635 10-11 25.85924630839752 24.863997394152335 27.738699492065543 21.5380568053846 12-13 26.43715771210473 22.31448014134296 27.483487836906633 23.764874309645677 14-15 23.567072939457876 23.897487914224318 24.703928748559154 27.831510397758652 16-17 23.918233004803078 26.945344019520757 25.483960154517632 23.652462821158533 18-19 23.914083636305858 26.43370282259171 26.668244723800278 22.98396881730215 20-21 24.417398370313524 25.648646818499877 26.502819222174573 23.43113558901203 22-23 24.315217803560497 25.3805850597901 26.225918253453667 24.078278883195743 24-25 24.111370855478025 25.554715961588336 26.23373958925554 24.100173593678097 26-27 23.86612727630923 25.798174275717123 26.29119892406034 24.044499523913313 28-29 24.002755315854714 26.07047000163405 26.056964259436644 23.869810423074597 30-31 24.321981965260143 25.72978368206684 25.919964206411137 24.028270146261875 32-33 24.039417939026052 25.855726948801998 25.83527049730121 24.26958461487074 34-35 24.394605167706544 25.834903774873247 25.910479957506904 23.860011099913304 36-37 24.40461994665126 25.686433984320345 25.970509250274194 23.9384368187542 38-39 24.24896919109982 25.78228276101879 25.939589669632024 24.029158378249363 40-41 24.345336069573733 25.63149182332437 25.985762378572108 24.03740972852978 42-43 24.230779323605926 25.658491120913922 25.789589412962332 24.321140142517812 44-45 24.134117447838268 25.668141253502537 26.00245611699048 24.195285181668712 46-47 24.29542470463012 25.62875731425047 25.813340309926463 24.262477671192947 48-49 24.076300006966303 25.738224461491278 25.857031577252222 24.3284439542902 50-51 24.14282227582859 25.80656367030865 25.95264333818637 24.09797071567639 52-53 24.294563047876295 25.595398033648042 25.930300663273307 24.179738255202356 54-55 23.88057028000693 25.458543751206598 26.194345147251653 24.466540821534824 56-57 23.935950489859657 25.66597916655735 26.060276974669005 24.33779336891398 58-59 24.084853939806187 25.532490483146887 26.054508220675544 24.32814735637138 60-61 24.07321718595193 25.48652964834578 26.196569867128723 24.24368329857357 62-63 24.025728941440423 25.50315279046835 26.284845798239502 24.186272469851726 64-65 23.85643549845679 25.33329425102926 26.18669978530064 24.623570465213312 66-67 23.84999391164999 25.494011252863018 26.037258530882045 24.618736304604948 68-69 23.891409624322474 25.443695772250553 26.128066547174218 24.536828056252755 70-71 23.84504406392095 25.23477390952763 26.114912627436226 24.805269399115193 72-73 23.806617774106968 25.388156686571673 26.047328745622693 24.757896793698666 74-75 23.762692410263426 25.491964896016405 26.09864591373232 24.64669677998785 76-77 23.789051298913343 25.11315684020186 26.21345435237055 24.88433750851424 78-79 23.69501071480982 25.161427842726898 26.258603377965255 24.884958064498033 80-81 23.841377796445734 25.25159664403891 26.101245989501265 24.805779570014092 82-83 23.707481337419065 25.28671815634638 26.19198294865069 24.813817557583864 84-85 23.729329436272486 25.2768983333261 26.1825270673459 24.811245163055514 86-87 23.658324294259984 25.313954332302824 26.155660535300168 24.872060838137028 88-89 23.582743827929093 25.0941628106305 26.225220503495045 25.097872857945365 90-91 23.6220736088613 25.016750676452222 26.272227903314842 25.088947811371636 92-93 23.644839837427657 24.883226008100383 26.381918323992558 25.090015830479402 94-95 23.55676761409819 24.820699414810527 26.45234514686205 25.170187824229227 96-97 23.528093645969236 24.657218857198295 26.45628590170011 25.358401595132356 98-99 23.400838850802636 24.598212039503522 26.434044296853422 25.56690481284042 100-101 23.515331946617472 24.46124785603254 26.558607327970368 25.46481286937962 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 60.0 1 48.0 2 75.5 3 128.5 4 119.0 5 129.0 6 190.5 7 357.0 8 593.5 9 645.5 10 541.0 11 491.5 12 527.5 13 600.0 14 676.5 15 756.5 16 858.0 17 978.5 18 1084.5 19 1177.5 20 1265.0 21 1287.5 22 1406.5 23 1590.0 24 1724.5 25 1985.5 26 2396.0 27 2979.5 28 3845.0 29 5340.0 30 7837.0 31 11510.0 32 16791.5 33 22005.5 34 27001.5 35 33281.5 36 41537.5 37 50305.5 38 58637.0 39 66757.5 40 73780.0 41 80576.0 42 87734.5 43 95035.5 44 101757.0 45 109484.5 46 115957.5 47 120836.0 48 125054.5 49 126758.5 50 126419.0 51 125208.5 52 124101.0 53 123385.5 54 121154.0 55 114911.0 56 105754.5 57 94770.0 58 85130.0 59 74667.0 60 61615.5 61 50572.5 62 41211.0 63 31062.5 64 21982.5 65 15892.5 66 11781.5 67 8965.5 68 6753.0 69 4939.0 70 3634.0 71 2647.0 72 1832.5 73 1272.0 74 914.0 75 619.5 76 430.0 77 292.5 78 197.0 79 140.5 80 116.5 81 112.0 82 90.0 83 66.0 84 60.5 85 51.0 86 46.0 87 44.0 88 37.5 89 32.5 90 26.0 91 22.5 92 18.5 93 16.5 94 15.5 95 15.5 96 14.0 97 7.5 98 5.5 99 5.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05546071388337652 2 0.0767639828880589 3 0.07290050867873515 4 0.07279218697193168 5 0.06856764040659637 6 0.011662637099173577 7 0.014623430418468416 8 0.01787308162257251 9 0.006174337287797777 10-11 0.013413838025829673 12-13 0.023830775496763348 14-15 0.043003717600977494 16-17 0.028253911857905028 18-19 0.010832170680346977 20-21 0.021321322622482965 22-23 0.03946520851206415 24-25 0.03589059218754965 26-27 0.021646287742893375 28-29 0.012113977544188035 30-31 0.011445993685566638 32-33 0.008322717806066594 34-35 0.004946691277358453 36-37 5.596621518179271E-4 38-39 0.003646830795716815 40-41 0.0012456996282399022 42-43 0.24247814067956705 44-45 0.20999968225632673 46-47 0.21669757446034127 48-49 0.22478559523500036 50-51 0.21546992844990193 52-53 0.22341352028215639 54-55 0.2194778316016303 56-57 0.22149983679529509 58-59 0.23121268317200622 60-61 0.23525669355933576 62-63 0.22036245887385864 64-65 0.22130124699948872 66-67 0.21857515071160138 68-69 0.2128341002510175 70-71 0.20384339858632952 72-73 0.21611985869072278 74-75 0.21545187483210135 76-77 0.20853733921447987 78-79 0.22734920896268243 80-81 0.23915627500426065 82-83 0.23756755663780973 84-85 0.23180845255942528 86-87 0.2521909870562782 88-89 0.24408491266381854 90-91 0.25105360913484176 92-93 0.24623329318208734 94-95 0.24980790950660184 96-97 0.2572279464226395 98-99 0.25054810783642556 100-101 0.25730016089384183 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2769528.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.996380033707844 #Duplication Level Percentage of deduplicated Percentage of total 1 79.67461460836003 48.59863071690739 2 11.18826907477632 13.648878248088744 3 3.629890679648366 6.642305741299374 4 1.6946236398957617 4.134636302127485 5 0.9887471322412985 3.0154997917714517 6 0.5875153030479103 2.1501784020197623 7 0.42329627532780545 1.8073678333723495 8 0.29759065891639136 1.452156234059659 9 0.22816074033985595 1.252528130388779 >10 1.2198341114906848 13.045502966922326 >50 0.052654690351678954 2.1436719456075766 >100 0.013495700316811342 1.426599867075222 >500 9.50825663364007E-4 0.4000700279496175 >1k 3.565596236706995E-4 0.2819737924103666 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.6107235601156584E-5 0.0 5 0.0 0.0 0.0 3.6107235601156584E-5 0.0 6 0.0 0.0 0.0 3.6107235601156584E-5 0.0 7 0.0 0.0 0.0 3.6107235601156584E-5 3.6107235601156584E-5 8 0.0 0.0 0.0 3.6107235601156584E-5 3.6107235601156584E-5 9 0.0 0.0 0.0 7.221447120231317E-5 3.6107235601156584E-5 10-11 0.0 0.0 0.0 7.221447120231317E-5 7.221447120231317E-5 12-13 0.0 0.0 0.0 1.0832170680346976E-4 2.1664341360693953E-4 14-15 0.0 1.8053617800578292E-5 0.0 1.0832170680346976E-4 2.8885788480925267E-4 16-17 0.0 3.6107235601156584E-5 0.0 1.0832170680346976E-4 3.2496512041040927E-4 18-19 0.0 3.6107235601156584E-5 0.0 1.0832170680346976E-4 3.2496512041040927E-4 20-21 0.0 3.6107235601156584E-5 0.0 1.0832170680346976E-4 3.6107235601156586E-4 22-23 0.0 3.6107235601156584E-5 0.0 1.9858979580636123E-4 4.1523320941330076E-4 24-25 0.0 5.4160853401734876E-5 0.0 3.06911502609831E-4 4.5134044501445736E-4 26-27 0.0 7.221447120231317E-5 0.0 5.957693874190837E-4 4.6939406281503566E-4 28-29 0.0 7.221447120231317E-5 0.0 0.0032496512041040927 4.6939406281503566E-4 30-31 3.6107235601156584E-5 7.221447120231317E-5 0.0 0.013269409083425046 4.6939406281503566E-4 32-33 3.6107235601156584E-5 7.221447120231317E-5 0.0 0.03865279571103812 5.596621518179271E-4 34-35 5.4160853401734876E-5 7.221447120231317E-5 0.0 0.07396567212896926 5.957693874190837E-4 36-37 7.221447120231317E-5 7.221447120231317E-5 0.0 0.1324955010384441 6.138230052196619E-4 38-39 7.221447120231317E-5 9.026808900289147E-5 0.0 0.22882960562232987 7.040910942225534E-4 40-41 7.221447120231317E-5 1.2637532460404804E-4 0.0 0.33030899127938046 7.221447120231317E-4 42-43 7.221447120231317E-5 1.4442894240462633E-4 0.0 0.442494172292174 7.401983298237101E-4 44-45 7.221447120231317E-5 1.4442894240462633E-4 0.0 0.5826444072780632 7.582519476242883E-4 46-47 7.221447120231317E-5 1.4442894240462633E-4 0.0 0.7435201954990165 8.485200366271798E-4 48-49 7.221447120231317E-5 1.4442894240462633E-4 0.0 0.9068512757408482 9.387881256300713E-4 50-51 7.221447120231317E-5 1.4442894240462633E-4 0.0 1.0797146661813855 9.387881256300713E-4 52-53 7.221447120231317E-5 1.4442894240462633E-4 0.0 1.2579219274908937 9.929489790318062E-4 54-55 7.221447120231317E-5 1.4442894240462633E-4 0.0 1.4597613745013591 0.0010290562146329629 56-57 7.221447120231317E-5 1.4442894240462633E-4 0.0 1.7005966359610736 0.0010832170680346977 58-59 7.221447120231317E-5 1.4442894240462633E-4 0.0 1.9503864918498746 0.0011193243036358542 60-61 7.221447120231317E-5 1.4442894240462633E-4 0.0 2.2153594403089625 0.0011193243036358542 62-63 7.221447120231317E-5 1.4442894240462633E-4 1.8053617800578292E-5 2.487174709914469 0.0012276460104393239 64-65 7.221447120231317E-5 1.4442894240462633E-4 3.6107235601156584E-5 2.7826402188387336 0.0012456996282399022 66-67 7.221447120231317E-5 2.1664341360693953E-4 3.6107235601156584E-5 3.125243723840308 0.0012637532460404806 68-69 1.0832170680346976E-4 2.1664341360693953E-4 3.6107235601156584E-5 3.4984120037782613 0.0012637532460404806 70-71 1.0832170680346976E-4 2.1664341360693953E-4 3.6107235601156584E-5 3.8801918594070903 0.0013359677172427938 72-73 1.0832170680346976E-4 2.1664341360693953E-4 3.6107235601156584E-5 4.277100646752803 0.0014081821884451068 74-75 1.0832170680346976E-4 2.1664341360693953E-4 3.6107235601156584E-5 4.701631469333403 0.0014442894240462635 76-77 1.0832170680346976E-4 2.1664341360693953E-4 3.6107235601156584E-5 5.179167713776499 0.0014623430418468418 78-79 1.0832170680346976E-4 2.1664341360693953E-4 3.6107235601156584E-5 5.708698377485261 0.0014803966596474202 80-81 1.0832170680346976E-4 2.527506492080961E-4 3.6107235601156584E-5 6.24814047736654 0.001534557513049155 82-83 1.0832170680346976E-4 2.527506492080961E-4 3.6107235601156584E-5 6.805582034194996 0.0016248256020520463 84-85 1.0832170680346976E-4 2.527506492080961E-4 3.6107235601156584E-5 7.40133336799628 0.0016248256020520463 86-87 1.0832170680346976E-4 2.527506492080961E-4 3.6107235601156584E-5 8.042056263738804 0.0016609328376532028 88-89 1.0832170680346976E-4 2.527506492080961E-4 3.6107235601156584E-5 8.72785904312937 0.0016970400732543595 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2105 0.0 46.444336 1 GTATCAA 3835 0.0 43.93196 1 TCAACGC 4535 0.0 36.006847 4 CAACGCA 4630 0.0 35.36865 5 TATCAAC 4800 0.0 34.70996 2 ATCAACG 4995 0.0 32.87918 3 AACGCAG 5090 0.0 32.05874 6 GTACATG 7450 0.0 28.98496 1 TACATGG 7710 0.0 26.903904 2 ACGCAGA 5990 0.0 26.37508 7 CGCAGAG 6305 0.0 24.909576 8 ACATGGG 8265 0.0 24.867117 3 CATGGGG 7180 0.0 22.676373 4 GCAGAGT 7015 0.0 20.223995 9 GTATATA 740 0.0 19.881428 1 TAAGGCG 800 0.0 19.579624 5 GTATTAA 1305 0.0 19.274494 1 GTATAGC 940 0.0 19.185509 1 ATGGGGG 3970 0.0 19.010214 5 GAGTACA 5570 0.0 17.978144 1 >>END_MODULE