FastQCFastQC Report
Mon 15 Aug 2016
SRR2921681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2769528
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19950.047.182141
GTATCAA36000.045.6908151
TCAACGC46450.034.2294654
TATCAAC48550.032.9450042
CAACGCA48750.032.5442165
ATCAACG49850.031.9906443
AACGCAG52200.030.2973796
GTACATG73950.027.8358861
TACATGG77100.025.7318362
ACGCAGA63900.024.5261127
ACATGGG82350.023.97613
CGCAGAG66250.023.0109628
CATGGGG71700.021.182244
GTATAGC7200.019.8081591
GCAGAGT78050.018.436799
ATGGGGG40450.018.3192635
AGAGTAC63150.017.56018610-11
TATACCG2807.1397335E-716.9646135
TAAGGTG16100.016.8170955
GTATAGG6550.016.6932891
Produced by FastQC (version 0.11.5)