##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921681_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2769528
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28137754880976	33.0	31.0	34.0	28.0	34.0
2	31.417666837092817	34.0	31.0	34.0	28.0	34.0
3	31.488812534121337	34.0	31.0	34.0	28.0	34.0
4	34.96937239847367	37.0	35.0	37.0	32.0	37.0
5	34.700343163167155	37.0	35.0	37.0	32.0	37.0
6	34.673874754109725	37.0	35.0	37.0	32.0	37.0
7	34.5550057627148	37.0	35.0	37.0	32.0	37.0
8	34.49569782287812	37.0	35.0	37.0	31.0	37.0
9	36.07331213116459	39.0	37.0	39.0	31.0	39.0
10-11	35.96689580318379	39.0	37.0	39.0	31.0	39.0
12-13	35.88750285247161	39.0	37.0	39.0	30.0	39.0
14-15	37.11381253412134	40.0	38.0	41.0	31.0	41.0
16-17	37.07855454070152	40.0	38.0	41.0	31.0	41.0
18-19	37.03698409981773	40.0	38.0	41.0	31.0	41.0
20-21	36.98208340915853	40.0	37.5	41.0	30.5	41.0
22-23	36.88787259056417	40.0	37.0	41.0	30.0	41.0
24-25	36.80265265417067	40.0	37.0	41.0	30.0	41.0
26-27	36.67223927687317	40.0	37.0	41.0	30.0	41.0
28-29	36.55138763717139	40.0	37.0	41.0	30.0	41.0
30-31	36.393793996666574	40.0	37.0	41.0	29.5	41.0
32-33	36.34294291301623	40.0	36.5	41.0	29.5	41.0
34-35	36.4739433939646	40.0	37.0	41.0	30.0	41.0
36-37	36.47054859167338	40.0	37.0	41.0	30.0	41.0
38-39	36.36655957260587	40.0	37.0	41.0	29.5	41.0
40-41	36.279459532454624	40.0	36.5	41.0	29.0	41.0
42-43	36.159671431377475	40.0	36.0	41.0	28.5	41.0
44-45	36.02897569549757	40.0	36.0	41.0	28.0	41.0
46-47	35.87148080828213	40.0	36.0	41.0	27.5	41.0
48-49	35.725814109841096	40.0	35.0	41.0	27.0	41.0
50-51	35.56601955279022	39.5	35.0	41.0	27.0	41.0
52-53	35.38812191102599	39.0	35.0	41.0	26.0	41.0
54-55	35.173889197003966	39.0	35.0	41.0	26.0	41.0
56-57	34.94597382658706	39.0	35.0	41.0	26.0	41.0
58-59	34.6967122195551	38.5	35.0	40.0	25.5	41.0
60-61	34.41305341560006	38.0	34.0	40.0	25.0	41.0
62-63	34.10602113428715	37.0	34.0	40.0	24.0	41.0
64-65	33.76677885184768	37.0	34.0	40.0	23.0	41.0
66-67	33.40857647945787	36.0	34.0	39.0	22.5	41.0
68-69	33.04336244298668	36.0	33.0	39.0	22.0	40.5
70-71	32.64484345346933	35.0	33.0	38.5	21.0	40.0
72-73	32.27938767905578	35.0	33.0	37.5	20.0	39.5
74-75	31.881349637916642	35.0	32.5	37.0	19.0	39.0
76-77	29.18109963141734	33.0	28.5	34.5	11.5	36.5
78-79	30.75147028663368	34.5	31.5	36.0	13.0	37.0
80-81	30.933430353475394	35.0	32.0	35.5	15.0	37.0
82-83	30.764625596852603	35.0	32.0	35.0	12.5	37.0
84-85	30.572915854253864	35.0	32.0	35.0	9.5	36.0
86-87	30.365573664537784	35.0	32.0	35.0	7.0	36.0
88-89	30.10046007839603	35.0	31.0	35.0	2.0	36.0
90-91	29.906892257453258	34.0	31.0	35.0	2.0	35.0
92-93	29.754671915214434	34.0	31.0	35.0	2.0	35.0
94-95	29.64812469850458	34.0	31.0	35.0	2.0	35.0
96-97	29.446171694238153	34.0	31.0	35.0	2.0	35.0
98-99	29.241902771880262	34.0	31.0	35.0	2.0	35.0
100-101	28.11387662446453	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57440.0
3	18631.0
4	10211.0
5	6994.0
6	6393.0
7	6174.0
8	6412.0
9	7034.0
10	7875.0
11	8396.0
12	8817.0
13	9426.0
14	10098.0
15	10975.0
16	11434.0
17	12073.0
18	11512.0
19	10720.0
20	10233.0
21	10255.0
22	10779.0
23	12094.0
24	14345.0
25	17327.0
26	20800.0
27	24923.0
28	30509.0
29	36418.0
30	44277.0
31	55697.0
32	69631.0
33	92826.0
34	132063.0
35	197280.0
36	344993.0
37	665765.0
38	679870.0
39	78826.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.225393410658555	19.124628055173883	13.29550620845403	26.354472325713534
2	15.121495738793818	20.803380801228805	37.792022519417614	26.283100940559756
3	17.898551666659447	24.683091318522113	28.98392778988694	28.434429224931495
4	13.18372246026341	16.645747287642422	35.0463030835261	35.12422716856807
5	14.257089394431343	37.24851642523401	34.200177042991974	14.294217137342672
6	33.97951015158501	35.19566386191721	16.825680301891808	13.999145684605965
7	29.092719048155498	31.700383603271025	20.88590546836862	18.320991880204858
8	26.61959005288988	35.220261358614174	19.637642226401034	18.522506362094912
9	26.953654196671778	15.50513300461306	18.985581658679745	38.55563114003541
10-11	25.78422456294993	25.167653643747183	27.703713604749776	21.344408188553114
12-13	26.269757291482932	22.560678934636762	27.55996491083007	23.609598863050234
14-15	23.435470839671225	24.109699346877242	24.697464692102493	27.757365121349036
16-17	23.80022877544477	27.21985479114131	25.46134214927598	23.518574284137948
18-19	23.777688792184257	26.61646362665721	26.70165991030108	22.904187670857453
20-21	24.33552589057819	25.790524787499354	26.522907751918112	23.351041570004345
22-23	24.172927661319907	25.517416686164573	26.273827164773206	24.035828487742318
24-25	23.995632450646166	25.770292395802663	26.24394907919848	23.990126074352688
26-27	23.751080086934135	25.980003993568406	26.323700023073147	23.945215896424312
28-29	23.903242585568353	26.206141122948303	26.049048171260026	23.84156812022332
30-31	24.227865092823034	25.86806097882693	25.93367892600358	23.970395002346464
32-33	23.99963671903847	25.960180289913374	25.83852089037058	24.201662100677577
34-35	24.20590734913174	25.961223011827588	25.96564659940767	23.867223039632997
36-37	24.2793261554216	25.842125179911186	26.027434605488075	23.851114059179135
38-39	24.170616798241433	25.894556762018656	25.957076440462057	23.977749999277854
40-41	24.332887393999137	25.71956012688509	25.985083600084497	23.96246887903128
42-43	24.20547617985801	25.744899088555183	25.78362750378302	24.265997227803787
44-45	24.083910291212142	25.762632484255715	25.97197850678059	24.18147871775155
46-47	24.209314971940117	25.772739122867705	25.795198585703794	24.222747319488384
48-49	23.955941576819225	25.86463436762838	25.887311762725112	24.29211229282728
50-51	24.105869396450558	25.882702341620178	25.896134611565476	24.11529365036379
52-53	24.31192221623432	25.691029609429233	25.818075463829302	24.178972710507146
54-55	23.74796527205053	25.62661315831664	26.137967193035923	24.487454376596908
56-57	23.880645790302314	25.74738563979605	25.92455909754019	24.447409472361446
58-59	23.983703064107793	25.68470116476073	25.923099104603846	24.408496666527633
60-61	23.949599252380725	25.72028408376918	26.025918238530416	24.304198425319683
62-63	23.93515995488521	25.653490852265687	26.06137301065355	24.34997618219555
64-65	23.709774307355374	25.514961470677584	26.025543172599736	24.74972104936731
66-67	23.710219091367765	25.6702687831246	25.87219348063949	24.747318644868145
68-69	23.777666512050658	25.625201398191138	25.910301761322007	24.686830328436198
70-71	23.73272838494027	25.442700024822777	25.895544148237164	24.929027441999786
72-73	23.689033431132923	25.679597570890856	25.747430006714616	24.88393899126161
74-75	23.63094114810805	25.686238215671388	25.801578136159613	24.88124250006095
76-77	23.60698948674384	25.348516151891644	25.97697351336963	25.067520847994896
78-79	23.512458614783522	25.46199751620942	25.969452592142467	25.05609127686459
80-81	23.686411214031413	25.55662685166668	25.772873301078832	24.984088633223077
82-83	23.50821312796646	25.55948610365944	25.870779734744886	25.06152103362921
84-85	23.535410752591837	25.531155870025586	25.87514459303338	25.058288784349198
86-87	23.433374186489843	25.600693154735666	25.796239100806613	25.169693557967882
88-89	23.28642107247357	25.41254728995276	25.87943060660608	25.421601030967594
90-91	23.22036501024219	25.37716469500871	25.924024529105495	25.47844576564361
92-93	23.239225966061234	25.304023346449693	26.00475281462289	25.451997872866183
94-95	23.10706884242844	25.251052907353184	26.033217814148806	25.608660436069574
96-97	23.02021330092801	25.12776297649862	26.108102203158616	25.74392151941475
98-99	22.876542449833426	25.104436777994284	26.011297713286357	26.007723058885933
100-101	22.795491715452133	25.0957430041666	26.079287646412823	26.029477633968444
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	38.5
2	61.0
3	90.0
4	90.0
5	107.0
6	167.5
7	354.0
8	535.5
9	523.0
10	430.0
11	375.0
12	381.5
13	396.0
14	424.5
15	447.0
16	480.5
17	548.0
18	601.0
19	655.5
20	711.5
21	826.0
22	991.5
23	1199.5
24	1410.0
25	1713.5
26	2234.0
27	3006.0
28	4077.0
29	5868.5
30	8705.5
31	12717.0
32	18186.0
33	23276.5
34	28044.5
35	34197.5
36	42306.0
37	50735.0
38	58935.0
39	66591.0
40	73669.0
41	80923.5
42	88223.0
43	95641.0
44	102289.0
45	109652.5
46	115874.0
47	121590.0
48	126211.5
49	128027.0
50	127837.5
51	126482.5
52	125191.5
53	123639.0
54	121245.0
55	114921.0
56	105382.5
57	94338.0
58	84411.0
59	73921.5
60	60830.5
61	49659.5
62	40332.0
63	30323.5
64	21250.0
65	15127.5
66	11044.5
67	8362.0
68	6284.0
69	4505.5
70	3275.5
71	2329.5
72	1505.5
73	931.0
74	661.0
75	448.5
76	287.5
77	165.5
78	89.5
79	59.5
80	34.0
81	22.5
82	21.5
83	14.0
84	8.5
85	6.5
86	4.5
87	2.5
88	3.0
89	2.0
90	1.5
91	2.5
92	2.5
93	1.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1685846830218001
2	2.5275064920809607E-4
3	0.009965597025919218
4	0.005993801109791993
5	0.02560003004122002
6	0.0021303269004682387
7	0.0
8	0.0
9	0.0
10-11	0.05038764728141402
12-13	4.513404450144573E-4
14-15	0.005073066601962501
16-17	0.0
18-19	0.0014803966596474202
20-21	2.5275064920809607E-4
22-23	0.0
24-25	4.1523320941330076E-4
26-27	0.0027080426700867443
28-29	0.005361924486771753
30-31	0.01691623987914186
32-33	0.011608476245771843
34-35	0.010200294057326736
36-37	0.0038273669737225982
38-39	0.0
40-41	0.0033579729109075624
42-43	0.008792111868881628
44-45	0.006842321146419173
46-47	0.003394080146508719
48-49	0.009044862518089725
50-51	0.0028163643768902135
52-53	0.0020039515758641904
54-55	0.004188439329734164
56-57	0.0075103050050405705
58-59	0.01511087809908403
60-61	0.0073658760626359445
62-63	0.021339376240283543
64-65	0.04033178216649191
66-67	0.06010049365812514
68-69	0.049827985129596096
70-71	0.06892871276260792
72-73	0.03397690870068835
74-75	0.0272790164966738
76-77	0.23442622714050912
78-79	0.0861699177621602
80-81	0.06688865395114259
82-83	0.027423445439078428
84-85	0.05105563114003542
86-87	0.06831488975738825
88-89	0.29746946050012857
90-91	0.3033007790497153
92-93	0.053583137632116376
94-95	0.010507205559936568
96-97	0.015724701104303695
98-99	0.0010651634502341193
100-101	1.6248256020520463E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2769528.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.7929964305757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.6133884329205	47.005300539650754
2	11.771915973264463	14.07756259540878
3	3.8010693227148438	6.818319733363781
4	1.7921725500329628	4.286374675483867
5	1.0088767034387527	3.0161880563802153
6	0.6454285957632633	2.315526583359857
7	0.44377726718356064	1.8574340786874095
8	0.31989739985488386	1.5302099270138811
9	0.24606655717150983	1.3241751097176122
>10	1.2867426311810424	13.449409834660553
>50	0.055125191482693056	2.190846231353512
>100	0.01432632812662267	1.5030666631913179
>500	9.097851487410643E-4	0.387210517113774
>1k	3.032617162365824E-4	0.23837545461463877
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	3.6107235601156584E-5
3	0.0	0.0	0.0	0.0	3.6107235601156584E-5
4	0.0	0.0	0.0	0.0	3.6107235601156584E-5
5	0.0	0.0	0.0	0.0	3.6107235601156584E-5
6	0.0	0.0	0.0	0.0	3.6107235601156584E-5
7	0.0	0.0	0.0	0.0	3.6107235601156584E-5
8	0.0	0.0	0.0	0.0	3.6107235601156584E-5
9	0.0	0.0	0.0	3.6107235601156584E-5	3.6107235601156584E-5
10-11	0.0	0.0	0.0	3.6107235601156584E-5	3.6107235601156584E-5
12-13	0.0	1.8053617800578292E-5	0.0	3.6107235601156584E-5	1.0832170680346976E-4
14-15	0.0	5.4160853401734876E-5	0.0	3.6107235601156584E-5	1.8053617800578293E-4
16-17	0.0	7.221447120231317E-5	0.0	3.6107235601156584E-5	2.3469703140751783E-4
18-19	1.8053617800578292E-5	7.221447120231317E-5	0.0	5.4160853401734876E-5	2.527506492080961E-4
20-21	3.6107235601156584E-5	7.221447120231317E-5	0.0	7.221447120231317E-5	2.527506492080961E-4
22-23	3.6107235601156584E-5	7.221447120231317E-5	0.0	1.2637532460404804E-4	3.2496512041040927E-4
24-25	5.4160853401734876E-5	7.221447120231317E-5	0.0	2.8885788480925267E-4	4.1523320941330076E-4
26-27	7.221447120231317E-5	7.221447120231317E-5	0.0	6.318766230202403E-4	4.6939406281503566E-4
28-29	7.221447120231317E-5	7.221447120231317E-5	0.0	0.0033399192931069842	4.6939406281503566E-4
30-31	7.221447120231317E-5	7.221447120231317E-5	0.0	0.013413838025829673	4.8744768061561395E-4
32-33	7.221447120231317E-5	7.221447120231317E-5	0.0	0.039230511480656635	5.596621518179271E-4
34-35	1.0832170680346975E-4	7.221447120231317E-5	0.0	0.0756807658200242	5.777157696185053E-4
36-37	1.4442894240462633E-4	1.0832170680346975E-4	0.0	0.13509522200172738	5.777157696185053E-4
38-39	1.4442894240462633E-4	1.4442894240462633E-4	0.0	0.23191677426622875	6.138230052196619E-4
40-41	1.4442894240462633E-4	1.4442894240462633E-4	0.0	0.3340280365462996	6.499302408208185E-4
42-43	1.4442894240462633E-4	2.1664341360693953E-4	0.0	0.4471339520669226	6.499302408208185E-4
44-45	1.4442894240462633E-4	2.1664341360693953E-4	0.0	0.5889631735082657	6.679838586213969E-4
46-47	1.4442894240462633E-4	2.1664341360693953E-4	0.0	0.7506333209124443	7.401983298237101E-4
48-49	1.4442894240462633E-4	2.1664341360693953E-4	0.0	0.9142352054212848	8.124128010260233E-4
50-51	1.4442894240462633E-4	2.1664341360693953E-4	0.0	1.088849796788478	8.304664188266015E-4
52-53	1.6248256020520463E-4	2.1664341360693953E-4	0.0	1.2679055781346136	8.665736544277581E-4
54-55	1.8053617800578293E-4	2.1664341360693953E-4	0.0	1.4695825425848736	9.20734507829493E-4
56-57	1.8053617800578293E-4	2.1664341360693953E-4	0.0	1.7105983402225937	9.387881256300713E-4
58-59	1.8053617800578293E-4	2.1664341360693953E-4	0.0	1.9613450378548256	9.568417434306496E-4
60-61	1.8053617800578293E-4	2.1664341360693953E-4	0.0	2.226751273141127	0.0010290562146329629
62-63	1.8053617800578293E-4	2.1664341360693953E-4	0.0	2.4997039206680705	0.0010832170680346977
64-65	1.8053617800578293E-4	2.1664341360693953E-4	0.0	2.7959457351577597	0.0011012706858352758
66-67	2.1664341360693953E-4	2.527506492080961E-4	0.0	3.1398129934053745	0.0011373779214364325
68-69	2.1664341360693953E-4	2.527506492080961E-4	0.0	3.515183814714998	0.0011554315392370107
70-71	2.3469703140751783E-4	2.527506492080961E-4	0.0	3.898137155500865	0.001173485157037589
72-73	2.527506492080961E-4	2.527506492080961E-4	0.0	4.297735931898865	0.001354021335043372
74-75	2.527506492080961E-4	2.527506492080961E-4	0.0	4.724902582678348	0.0013720749528439503
76-77	2.527506492080961E-4	2.8885788480925267E-4	0.0	5.206446730273173	0.0013720749528439503
78-79	2.527506492080961E-4	2.8885788480925267E-4	0.0	5.7413393184687065	0.0014442894240462635
80-81	2.527506492080961E-4	2.8885788480925267E-4	0.0	6.285619787920541	0.0014984502774479983
82-83	2.527506492080961E-4	2.8885788480925267E-4	0.0	6.848188572204361	0.0016248256020520463
84-85	2.527506492080961E-4	2.8885788480925267E-4	0.0	7.44847136407359	0.0016248256020520463
86-87	2.527506492080961E-4	2.8885788480925267E-4	0.0	8.094357594507079	0.0016248256020520463
88-89	2.527506492080961E-4	2.8885788480925267E-4	0.0	8.78685826610166	0.0016248256020520463
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1995	0.0	47.18214	1
GTATCAA	3600	0.0	45.690815	1
TCAACGC	4645	0.0	34.229465	4
TATCAAC	4855	0.0	32.945004	2
CAACGCA	4875	0.0	32.544216	5
ATCAACG	4985	0.0	31.990644	3
AACGCAG	5220	0.0	30.297379	6
GTACATG	7395	0.0	27.835886	1
TACATGG	7710	0.0	25.731836	2
ACGCAGA	6390	0.0	24.526112	7
ACATGGG	8235	0.0	23.9761	3
CGCAGAG	6625	0.0	23.010962	8
CATGGGG	7170	0.0	21.18224	4
GTATAGC	720	0.0	19.808159	1
GCAGAGT	7805	0.0	18.43679	9
ATGGGGG	4045	0.0	18.319263	5
AGAGTAC	6315	0.0	17.560186	10-11
TATACCG	280	7.1397335E-7	16.964613	5
TAAGGTG	1610	0.0	16.817095	5
GTATAGG	655	0.0	16.693289	1
>>END_MODULE