FastQCFastQC Report
Mon 15 Aug 2016
SRR2921680_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921680_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750366
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.055.8313451
GTATCAA47650.043.3234831
TCAACGC57800.035.1434634
CAACGCA59950.034.04025
ATCAACG60850.033.2253653
TATCAAC62800.032.118112
AACGCAG64100.031.9675586
GTACATG75200.028.9031161
ACGCAGA72850.027.6756847
TACATGG78800.026.8614442
CGCAGAG77050.025.7981328
ACATGGG81950.025.018173
GCAGAGT85550.021.682619
GTATAGG6950.020.4848821
CATGGGG63050.020.4744594
AGAGTAC71350.019.81494510-11
GAGTACA58700.019.2413641
CAGAGTA77150.018.66351110-11
GAGTACT42550.018.06349812-13
TAAGGTG13600.017.447935
Produced by FastQC (version 0.11.5)