##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921680_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2750366
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.2007198314697	31.0	31.0	34.0	26.0	34.0
2	30.25953600357189	31.0	31.0	34.0	26.0	34.0
3	30.31648333349089	31.0	31.0	34.0	26.0	34.0
4	33.771517681646735	37.0	35.0	37.0	28.0	37.0
5	33.76903219426069	37.0	35.0	37.0	28.0	37.0
6	33.810149630994566	37.0	35.0	37.0	29.0	37.0
7	33.722133345162064	37.0	35.0	37.0	28.0	37.0
8	33.69675744973578	37.0	35.0	37.0	28.0	37.0
9	35.22929966411743	39.0	35.0	39.0	29.0	39.0
10-11	35.12594069298413	39.0	35.0	39.0	28.0	39.0
12-13	35.020859405620925	39.0	35.0	39.0	27.0	39.0
14-15	36.13005214578714	40.0	36.0	41.0	27.0	41.0
16-17	36.07167955101248	40.0	36.0	41.0	27.0	41.0
18-19	36.04972756353155	40.0	36.0	41.0	27.0	41.0
20-21	35.979623075619756	40.0	36.0	41.0	27.0	41.0
22-23	35.87266676507781	40.0	36.0	41.0	27.0	41.0
24-25	35.75674692022807	40.0	36.0	41.0	27.0	41.0
26-27	35.6076165863016	40.0	36.0	41.0	26.0	41.0
28-29	35.464611073580755	40.0	36.0	41.0	25.0	41.0
30-31	35.30379774909957	39.5	35.0	41.0	25.0	41.0
32-33	35.11171931299326	39.0	35.0	41.0	24.0	41.0
34-35	34.928760572229294	39.0	35.0	41.0	24.0	41.0
36-37	34.87845981225772	39.0	35.0	41.0	23.5	41.0
38-39	35.03581741484588	39.5	35.0	41.0	24.0	41.0
40-41	35.08424478778461	40.0	35.0	41.0	23.5	41.0
42-43	34.90869669709413	40.0	35.0	41.0	22.5	41.0
44-45	34.76898092835644	40.0	35.0	41.0	21.0	41.0
46-47	34.59297235349768	39.0	35.0	41.0	19.0	41.0
48-49	34.413393890122265	39.0	35.0	41.0	17.0	41.0
50-51	32.72328719159559	37.0	32.0	39.5	13.5	40.0
52-53	32.838496949133315	37.5	32.5	39.5	13.0	40.0
54-55	33.745182459352684	38.5	34.0	40.5	13.0	41.0
56-57	33.7674720382669	38.5	34.0	41.0	10.5	41.0
58-59	33.58004298337022	38.0	34.0	41.0	8.0	41.0
60-61	33.329838646929176	38.0	33.0	40.0	7.0	41.0
62-63	33.04110852882853	37.0	33.0	40.0	3.5	41.0
64-65	32.748416937963896	37.0	33.0	40.0	2.0	41.0
66-67	32.40557220384487	36.0	32.5	39.5	2.0	41.0
68-69	32.039724531207845	36.0	32.0	39.0	2.0	41.0
70-71	31.62334685638202	35.0	32.0	39.0	2.0	40.5
72-73	31.25856940494465	35.0	32.0	37.5	2.0	40.0
74-75	30.849381682292467	35.0	31.0	37.0	2.0	39.0
76-77	30.466655346961097	35.0	31.0	36.5	2.0	39.0
78-79	30.104954395160497	35.0	31.0	36.0	2.0	38.5
80-81	29.768924390426584	35.0	31.0	36.0	2.0	37.0
82-83	29.44435922346335	35.0	30.0	35.0	2.0	37.0
84-85	29.162272766606335	35.0	30.0	35.0	2.0	36.5
86-87	28.90630683334509	35.0	30.0	35.0	2.0	36.0
88-89	28.6585305373903	34.0	30.0	35.0	2.0	36.0
90-91	28.409262076392743	34.0	29.0	35.0	2.0	35.5
92-93	28.193704583317274	34.0	29.0	35.0	2.0	35.0
94-95	27.940737160072516	34.0	29.0	35.0	2.0	35.0
96-97	27.68168109262549	34.0	29.0	35.0	2.0	35.0
98-99	27.38377255972478	34.0	29.0	35.0	2.0	35.0
100-101	26.287481738794035	33.0	25.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	102099.0
3	17025.0
4	11586.0
5	8789.0
6	8154.0
7	8423.0
8	8722.0
9	9830.0
10	10686.0
11	11487.0
12	11502.0
13	11929.0
14	12959.0
15	13703.0
16	14384.0
17	15294.0
18	14298.0
19	13062.0
20	13211.0
21	13702.0
22	14849.0
23	17031.0
24	19796.0
25	23246.0
26	26883.0
27	31632.0
28	37105.0
29	43047.0
30	51341.0
31	61733.0
32	75943.0
33	97506.0
34	132354.0
35	192002.0
36	314559.0
37	561652.0
38	608345.0
39	110481.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.846742637118616	18.492950347571366	12.85337392334367	24.80693309196635
2	16.264519683308794	20.24535635765816	38.32086562456562	25.169258334467422
3	18.862077936727122	23.909098682618605	29.888420106576717	27.340403274077552
4	13.234044132522737	16.113057387416365	36.891816955260545	33.761081524800346
5	14.050525275554538	37.06451867599924	34.68936281071633	14.195593237729895
6	32.89556074817332	35.656811601791674	17.324216434707925	14.123411215327094
7	28.362359911140235	31.99344279225339	21.54663846737633	18.09755882923005
8	26.493028925441013	33.89648866143513	20.630336856781593	18.98014555634226
9	26.71012318457469	15.145506733736166	19.80541651425251	38.338953567436626
10-11	25.206432531312377	25.28332370602735	28.438752788418725	21.07149097424155
12-13	25.842836582303452	23.032384235835135	27.894192767075165	23.23058641478625
14-15	23.10850689487225	24.6642953248143	25.307842196523595	26.919355583789855
16-17	23.07284694028594	27.535098907581396	26.29845864124321	23.093595510889454
18-19	23.04135618242792	26.864728111713422	27.576127538455804	22.51778816740286
20-21	23.673211035765373	26.187531786766776	27.454838470947646	22.684418706520205
22-23	23.57215538179368	26.057849428534794	27.07478378528294	23.29521140438858
24-25	23.30526344504003	26.233297416242706	27.136334910957437	23.32510422775982
26-27	23.107987213476036	26.383679362273938	27.15938060274132	23.34895282150871
28-29	23.239392318492076	26.53439435299388	27.06127395812235	23.16493937039169
30-31	23.495997338225376	26.29818784783522	26.81487030212233	23.39094451181708
32-33	23.241209136447466	26.43080612975105	26.674503877637694	23.653480856163785
34-35	23.698980171568248	26.418056026477903	26.696943059957984	23.18602074199586
36-37	23.761282225059848	26.108934009709333	26.710713278048125	23.419070487182694
38-39	23.39983445168438	26.326727217426605	26.811827778120172	23.461610552768843
40-41	23.638881672608054	26.146135777055886	26.917822327025448	23.297160223310613
42-43	23.494420065894978	26.145143890136165	26.64868647403563	23.71174956993323
44-45	23.479714620342417	26.099307938116855	26.83377999431252	23.58719744722821
46-47	23.626267817676563	26.262005906143372	26.50023383722051	23.61149243895956
48-49	23.32934071512201	26.291609562023023	26.53588564519532	23.843164077659644
50-51	23.423697354555962	26.385648054357603	26.6182958449141	23.572358746172334
52-53	23.652577218909467	26.18142271404188	26.591020728235794	23.57497933881286
54-55	23.14570831664015	26.187775870245538	26.82378392086966	23.84273189224465
56-57	23.249085423012012	26.288110107435354	26.69287612895303	23.769928340599602
58-59	23.253114679421476	26.042956383099874	26.84663705728277	23.857291880195877
60-61	23.164942855476838	26.076889996792907	26.947924866614187	23.810242281116068
62-63	23.21207401855237	26.076716469794732	26.987664424839643	23.723545086813257
64-65	23.054345348678957	25.917083566310684	26.897947813438115	24.130623271572247
66-67	23.01697742574084	26.04839511894911	26.728541554848423	24.20608590046163
68-69	23.182534139140568	25.875007332641786	26.790776866526244	24.151681661691402
70-71	23.063227206875275	25.681549946507776	26.816608838959173	24.438614007657776
72-73	23.064133687379588	25.734510855950848	26.7615607792054	24.439794677464167
74-75	22.9970502915185	25.862955060988153	26.71941077974435	24.420583867749
76-77	23.011093485055056	25.57352895097924	26.91560090560846	24.499776658357238
78-79	22.982932173637995	25.568760524588686	26.85931515150853	24.5889921502648
80-81	23.053034549466183	25.64225824157001	26.738662032761457	24.56604517620235
82-83	23.013817649592802	25.595652436948818	26.831365197191424	24.559164716266952
84-85	22.927680339063038	25.54986138101498	26.88750953204818	24.634948747873793
86-87	22.980627724069684	25.573627679418404	26.769170206115767	24.676574390396148
88-89	22.897751739940908	25.35897243849044	26.801276889105385	24.941998932463267
90-91	22.81151653837546	25.32168785980526	26.9084030679082	24.958392533911088
92-93	22.895428444485106	25.170007528351597	27.08309452245448	24.85146950470882
94-95	22.792334844281022	24.992017589005705	27.13042895071025	25.08521861600302
96-97	22.83558795863686	24.779244288164097	27.13073983532241	25.254427917876633
98-99	22.75374991616634	24.736561828419465	27.118914040607567	25.390774214806626
100-101	22.899125394613122	24.511259311830802	27.201253649049345	25.38836164450673
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	153.0
1	124.0
2	172.5
3	265.5
4	241.5
5	247.0
6	382.0
7	670.5
8	981.5
9	1070.0
10	920.0
11	806.0
12	865.5
13	941.5
14	1022.0
15	1133.5
16	1248.5
17	1351.0
18	1474.5
19	1612.5
20	1697.0
21	1858.0
22	2078.5
23	2302.0
24	2661.0
25	3174.0
26	3973.5
27	5365.0
28	7270.0
29	10151.5
30	14112.0
31	19478.0
32	27580.0
33	33839.0
34	38544.5
35	45079.5
36	53220.0
37	61676.5
38	68461.5
39	75198.0
40	81006.5
41	85813.0
42	91150.0
43	96690.0
44	101894.5
45	108443.0
46	112978.5
47	115350.0
48	118141.0
49	119253.0
50	118376.5
51	116203.0
52	113926.0
53	112274.5
54	109255.5
55	102999.5
56	94252.0
57	83919.0
58	75578.5
59	66106.5
60	54052.5
61	44155.0
62	36411.0
63	27866.0
64	19427.0
65	13721.5
66	9952.0
67	7439.0
68	5506.0
69	4039.5
70	2999.0
71	2188.5
72	1501.5
73	1044.5
74	771.5
75	543.0
76	393.5
77	284.5
78	201.5
79	142.0
80	126.5
81	118.5
82	90.0
83	66.0
84	57.0
85	52.0
86	40.5
87	34.5
88	34.5
89	29.5
90	23.5
91	20.0
92	19.5
93	18.0
94	15.0
95	9.0
96	5.5
97	8.0
98	8.0
99	7.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05737418219975087
2	0.07998935414413937
3	0.07675342118103555
4	0.07628075681563835
5	0.07268123587915208
6	0.011198509580179511
7	0.015088900895371743
8	0.01828847506113732
9	0.00669001871023711
10-11	0.013652728400511058
12-13	0.023578680073851988
14-15	0.04177625814164369
16-17	0.028323503126492983
18-19	0.010671307018774955
20-21	0.021724381409601486
22-23	0.039031168942606186
24-25	0.0355770831954729
26-27	0.02166984321359412
28-29	0.011162150782841265
30-31	0.011671173945576699
32-33	0.008653393766502348
34-35	0.005399281404729407
36-37	7.998935414413935E-4
38-39	0.004054005903214336
40-41	9.63508129463497E-4
42-43	0.24018621521644756
44-45	0.20875403491753464
46-47	0.21580764160115418
48-49	0.2230612216701341
50-51	0.2135715755648521
52-53	0.22142507578991308
54-55	0.2175165050760517
56-57	0.2204979264577878
58-59	0.2291876790216284
60-61	0.23436880764232831
62-63	0.2191344715576036
64-65	0.21817096342814013
66-67	0.21658935574392643
68-69	0.21048107779110126
70-71	0.20268211576204767
72-73	0.21464416008633033
74-75	0.21297165540877105
76-77	0.2069542744492915
78-79	0.22531546710510528
80-81	0.23749566421341742
82-83	0.23575044194118164
84-85	0.23027844294177574
86-87	0.2502757814778106
88-89	0.2423859224554114
90-91	0.2497122201190678
92-93	0.2449673970664268
94-95	0.24847602100956748
96-97	0.2547115547530765
98-99	0.24862145619892043
100-101	0.2550387839291207
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2750366.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.235505565216364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.58242693899673	47.33452215212146
2	11.932839838506926	14.375612810024387
3	3.995493488198683	7.220117107325325
4	1.7697329810911155	4.264030433258434
5	0.9807764820677257	2.953878362191191
6	0.5949349556442667	2.1501724698991183
7	0.398819534726263	1.6816167412464569
8	0.2845121351062999	1.3710185838053692
9	0.21917727168109877	1.1882028391304185
>10	1.1666882949842812	12.37331703435177
>50	0.055374779328833015	2.2387597720842414
>100	0.017528162923570196	1.7963524092721412
>500	9.081089701228264E-4	0.38556490673818716
>1k	7.870277740791158E-4	0.6668343785514946
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.8179398669122583E-5	0.0
12-13	0.0	0.0	0.0	3.6358797338245165E-5	0.0
14-15	0.0	0.0	0.0	7.271759467649033E-5	0.0
16-17	0.0	0.0	0.0	1.2725579068385807E-4	0.0
18-19	0.0	0.0	0.0	2.3633218269859357E-4	3.6358797338245165E-5
20-21	0.0	0.0	0.0	2.908703787059613E-4	3.6358797338245165E-5
22-23	0.0	0.0	0.0	4.181261693898194E-4	3.6358797338245165E-5
24-25	0.0	1.8179398669122583E-5	0.0	6.726377507575356E-4	3.6358797338245165E-5
26-27	0.0	3.6358797338245165E-5	0.0	0.0013634549001841937	3.6358797338245165E-5
28-29	0.0	3.6358797338245165E-5	0.0	0.004126723497890826	7.271759467649033E-5
30-31	0.0	3.6358797338245165E-5	0.0	0.01641599699821769	7.271759467649033E-5
32-33	0.0	3.6358797338245165E-5	0.0	0.04866625023724115	7.271759467649033E-5
34-35	0.0	3.6358797338245165E-5	0.0	0.10220457931780716	7.271759467649033E-5
36-37	0.0	3.6358797338245165E-5	0.0	0.19331972544744952	7.271759467649033E-5
38-39	0.0	3.6358797338245165E-5	0.0	0.33446457671451724	1.4543518935298066E-4
40-41	0.0	7.271759467649033E-5	0.0	0.484517333329455	1.4543518935298066E-4
42-43	0.0	7.271759467649033E-5	0.0	0.6471684132220948	1.4543518935298066E-4
44-45	0.0	7.271759467649033E-5	0.0	0.8520865950204446	1.4543518935298066E-4
46-47	1.8179398669122583E-5	7.271759467649033E-5	0.0	1.1003626426446518	1.6361458802210325E-4
48-49	3.6358797338245165E-5	7.271759467649033E-5	0.0	1.3422577213359967	2.908703787059613E-4
50-51	3.6358797338245165E-5	7.271759467649033E-5	0.0	1.5961330237502935	2.908703787059613E-4
52-53	3.6358797338245165E-5	7.271759467649033E-5	0.0	1.8513717810647745	2.908703787059613E-4
54-55	3.6358797338245165E-5	7.271759467649033E-5	0.0	2.1527134934041507	2.908703787059613E-4
56-57	3.6358797338245165E-5	7.271759467649033E-5	0.0	2.5033395555355176	2.908703787059613E-4
58-59	3.6358797338245165E-5	7.271759467649033E-5	0.0	2.8677819606554182	2.908703787059613E-4
60-61	3.6358797338245165E-5	7.271759467649033E-5	0.0	3.243441054754167	2.908703787059613E-4
62-63	3.6358797338245165E-5	7.271759467649033E-5	0.0	3.620227271570402	2.908703787059613E-4
64-65	3.6358797338245165E-5	1.0907639201473549E-4	0.0	4.0354265577744926	2.908703787059613E-4
66-67	3.6358797338245165E-5	1.0907639201473549E-4	0.0	4.528379132086421	2.908703787059613E-4
68-69	3.6358797338245165E-5	1.0907639201473549E-4	0.0	5.050164232687576	2.908703787059613E-4
70-71	3.6358797338245165E-5	1.0907639201473549E-4	0.0	5.58092995623128	2.908703787059613E-4
72-73	3.6358797338245165E-5	1.0907639201473549E-4	0.0	6.116167811847587	3.2722917604420645E-4
74-75	3.6358797338245165E-5	1.0907639201473549E-4	0.0	6.680529064131829	3.2722917604420645E-4
76-77	3.6358797338245165E-5	1.0907639201473549E-4	0.0	7.320153026906237	3.2722917604420645E-4
78-79	3.6358797338245165E-5	1.0907639201473549E-4	0.0	8.022768606069157	3.2722917604420645E-4
80-81	3.6358797338245165E-5	1.0907639201473549E-4	0.0	8.732855918085084	3.2722917604420645E-4
82-83	7.271759467649033E-5	1.0907639201473549E-4	0.0	9.460468170418046	3.6358797338245164E-4
84-85	7.271759467649033E-5	1.0907639201473549E-4	0.0	10.216476643472177	3.6358797338245164E-4
86-87	7.271759467649033E-5	1.0907639201473549E-4	0.0	11.026950594939002	3.6358797338245164E-4
88-89	7.271759467649033E-5	1.0907639201473549E-4	0.0	11.9006706743757	3.6358797338245164E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2465	0.0	55.831345	1
GTATCAA	4765	0.0	43.323483	1
TCAACGC	5780	0.0	35.143463	4
CAACGCA	5995	0.0	34.0402	5
ATCAACG	6085	0.0	33.225365	3
TATCAAC	6280	0.0	32.11811	2
AACGCAG	6410	0.0	31.967558	6
GTACATG	7520	0.0	28.903116	1
ACGCAGA	7285	0.0	27.675684	7
TACATGG	7880	0.0	26.861444	2
CGCAGAG	7705	0.0	25.798132	8
ACATGGG	8195	0.0	25.01817	3
GCAGAGT	8555	0.0	21.68261	9
GTATAGG	695	0.0	20.484882	1
CATGGGG	6305	0.0	20.474459	4
AGAGTAC	7135	0.0	19.814945	10-11
GAGTACA	5870	0.0	19.241364	1
CAGAGTA	7715	0.0	18.663511	10-11
GAGTACT	4255	0.0	18.063498	12-13
TAAGGTG	1360	0.0	17.44793	5
>>END_MODULE