FastQCFastQC Report
Mon 15 Aug 2016
SRR2921680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750366
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24150.055.4999121
GTATCAA47250.043.4552541
TCAACGC59650.033.5742534
CAACGCA61750.032.5383075
ATCAACG62150.032.2243083
TATCAAC63750.031.7877662
AACGCAG66100.030.5390326
ACGCAGA77050.025.1505477
GTACATG73950.024.0377481
CGCAGAG79650.023.7928818
TACATGG76150.022.7475812
ACATGGG79400.021.338313
GCAGAGT89050.020.2679399
AGAGTAC75500.019.56470710-11
TAAGGTG16450.019.3464135
CATGGGG58000.019.0648024
CAGAGTA82850.017.48506510-11
GAGTACT42550.017.39667512-13
GTAAGGT13600.017.098644
AGTACTT47450.016.80019212-13
Produced by FastQC (version 0.11.5)