##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921680_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2750366
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268457725262746	33.0	31.0	34.0	28.0	34.0
2	31.39752309329013	33.0	31.0	34.0	28.0	34.0
3	31.465795097816073	34.0	31.0	34.0	28.0	34.0
4	34.94919148942359	37.0	35.0	37.0	32.0	37.0
5	34.68449689968535	37.0	35.0	37.0	32.0	37.0
6	34.658013151704175	37.0	35.0	37.0	32.0	37.0
7	34.536147189137736	37.0	35.0	37.0	31.0	37.0
8	34.48055349724364	37.0	35.0	37.0	31.0	37.0
9	36.05511121065342	39.0	37.0	39.0	31.0	39.0
10-11	35.948308334236245	39.0	37.0	39.0	31.0	39.0
12-13	35.87297508767924	39.0	37.0	39.0	30.0	39.0
14-15	37.09375152252464	40.0	38.0	41.0	31.0	41.0
16-17	37.06181922696834	40.0	38.0	41.0	31.0	41.0
18-19	37.03118930353269	40.0	38.0	41.0	31.0	41.0
20-21	36.97237422219443	40.0	37.5	41.0	30.5	41.0
22-23	36.878879210985005	40.0	37.0	41.0	30.0	41.0
24-25	36.79427574366466	40.0	37.0	41.0	30.0	41.0
26-27	36.66127635376528	40.0	37.0	41.0	30.0	41.0
28-29	36.536081197920566	40.0	37.0	41.0	30.0	41.0
30-31	36.369336299241624	40.0	36.5	41.0	29.5	41.0
32-33	36.2960633239358	40.0	36.5	41.0	29.5	41.0
34-35	36.406402093394114	40.0	37.0	41.0	30.0	41.0
36-37	36.38101892620837	40.0	37.0	41.0	30.0	41.0
38-39	36.26528432943107	40.0	36.5	41.0	29.0	41.0
40-41	36.172154542340905	40.0	36.0	41.0	28.5	41.0
42-43	36.031759227680965	40.0	36.0	41.0	28.0	41.0
44-45	35.88318700129365	40.0	36.0	41.0	27.5	41.0
46-47	35.7082217421245	40.0	35.5	41.0	27.0	41.0
48-49	35.55206288908458	40.0	35.0	41.0	26.0	41.0
50-51	35.38982702665754	39.5	35.0	41.0	26.0	41.0
52-53	35.21046017148264	39.0	35.0	41.0	25.5	41.0
54-55	34.99489649741162	39.0	35.0	41.0	24.5	41.0
56-57	34.764372268999836	39.0	35.0	41.0	24.0	41.0
58-59	34.52096811842497	38.5	34.5	40.5	23.5	41.0
60-61	34.24726145538448	38.0	34.0	40.0	23.0	41.0
62-63	33.94416706721941	37.5	34.0	40.0	22.5	41.0
64-65	33.610037536822375	37.0	34.0	40.0	22.0	41.0
66-67	33.25802965859816	36.0	33.5	39.0	20.5	41.0
68-69	32.89499324817133	36.0	33.0	39.0	20.0	41.0
70-71	32.49203124238738	35.0	33.0	39.0	18.5	40.0
72-73	32.1347707905057	35.0	33.0	37.5	17.5	40.0
74-75	31.728178904189477	35.0	32.5	37.0	14.0	39.0
76-77	29.010594408162405	33.0	28.0	34.5	7.5	36.5
78-79	30.566115927843786	34.5	31.0	36.0	7.0	37.5
80-81	30.737425491734555	35.0	32.0	35.5	7.0	37.0
82-83	30.549395244123872	35.0	32.0	35.0	3.5	37.0
84-85	30.336800811237488	35.0	32.0	35.0	2.0	36.5
86-87	30.109929187606305	35.0	31.0	35.0	2.0	36.0
88-89	29.823569663092112	34.5	31.0	35.0	2.0	36.0
90-91	29.609023853552582	34.0	31.0	35.0	2.0	35.0
92-93	29.434579070567338	34.0	31.0	35.0	2.0	35.0
94-95	29.31266584156436	34.0	31.0	35.0	2.0	35.0
96-97	29.088506402420624	34.0	31.0	35.0	2.0	35.0
98-99	28.86106285490731	34.0	31.0	35.0	2.0	35.0
100-101	27.73961192801249	33.0	27.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57164.0
3	18520.0
4	9882.0
5	7206.0
6	6215.0
7	6224.0
8	6596.0
9	7106.0
10	7937.0
11	8895.0
12	9593.0
13	10386.0
14	11190.0
15	12397.0
16	13639.0
17	14493.0
18	13370.0
19	11801.0
20	11190.0
21	11189.0
22	11785.0
23	13009.0
24	15313.0
25	18339.0
26	21573.0
27	25967.0
28	30817.0
29	37219.0
30	45192.0
31	55634.0
32	69907.0
33	91905.0
34	128594.0
35	191046.0
36	333059.0
37	637762.0
38	680728.0
39	87521.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.874020915781095	20.022668353681677	13.38248694857882	25.72082378195841
2	15.413489957849109	21.67605878073283	36.83805411442951	26.072397146988553
3	17.598647010353965	25.090125371901244	29.645956558007143	27.665271059737652
4	12.895388276086756	17.29258296099786	35.826999266964535	33.985029495950855
5	13.747638348671565	37.53977302689601	34.68713461458032	14.025454009852108
6	32.8626440891934	36.250101352837675	17.029203797559024	13.85805076040989
7	28.212790588598025	32.24959878067138	21.506555854748058	18.03105477598254
8	26.489819900333266	34.399276314497776	20.377615197395546	18.73328858777341
9	26.511227790658904	15.2062473208082	19.72495994165134	38.557564946881556
10-11	25.110493373671595	25.61014405472985	28.37170081855768	20.907661753040877
12-13	25.761843254637363	23.22089623057408	27.954671264865137	23.06258924992342
14-15	22.977728125139194	24.82143684999459	25.324233446991013	26.876601577875203
16-17	22.941892097269964	27.84387605140552	26.223637145019975	22.99059470630454
18-19	22.9751095828824	27.06225885721111	27.600015343694206	22.362616216212285
20-21	23.642270971879817	26.27443979514648	27.439725519119257	22.643563713854444
22-23	23.423155318237647	26.15010147740337	27.133825098186932	23.29291810617205
24-25	23.22710294679609	26.36118476859068	27.1806461957654	23.231066088847825
26-27	23.009996014974387	26.55975938589883	27.173220627888778	23.257023971238006
28-29	23.168520128157276	26.663829312222738	27.07394277791787	23.09370778170212
30-31	23.41275930961803	26.486073301627915	26.821213004991456	23.279954383762597
32-33	23.186613988549237	26.56326192212099	26.663515191624832	23.586608897704938
34-35	23.55235443364778	26.536288522777994	26.762791230428963	23.148565813145268
36-37	23.638543278077016	26.281618336703637	26.720015882230065	23.35982250298928
38-39	23.400012943731852	26.456478883174096	26.806686819136072	23.33682135395798
40-41	23.59571867178035	26.30167314603522	26.884507255292938	23.218100926891488
42-43	23.476134625478643	26.27342934547736	26.58658119594408	23.66385483309992
44-45	23.39179957547963	26.261829982773744	26.762400403612514	23.58397003813411
46-47	23.566121442698577	26.36962138170471	26.48270092116193	23.581556254434783
48-49	23.25641012121819	26.43715818580511	26.536190102008145	23.770241590968553
50-51	23.394575137257753	26.442460822455732	26.542049958186382	23.620914082100132
52-53	23.59114409015165	26.26999828018699	26.511135698336798	23.627721931324558
54-55	23.099736495436577	26.305460220337373	26.69568113584393	23.89912214838212
56-57	23.181608523337054	26.370751198982887	26.573722425757058	23.873917851923
58-59	23.12344052253166	26.179657259057947	26.70416352187764	23.992738696532754
60-61	23.07612032233925	26.293222250745963	26.723726667602378	23.906930759312413
62-63	23.091896452498904	26.275946653380622	26.738824676039023	23.893332218081444
64-65	22.87732164464003	26.097538219622766	26.639057518170222	24.386082617566977
66-67	22.849742318159716	26.271778258459676	26.517445072411228	24.36103435096938
68-69	23.050634555298366	26.106856733139733	26.503319651661204	24.339189059900697
70-71	22.963300125271562	25.985881388968025	26.49933834928972	24.551480136470687
72-73	22.911891385835904	26.138556675233378	26.307666176135758	24.641885762794963
74-75	22.856325594073745	26.312688539096023	26.237222893331037	24.593762973499192
76-77	22.885326092007286	26.006617270686533	26.451370604514302	24.65668603279188
78-79	22.825657979130188	26.084753290691705	26.379774234558575	24.709814495619536
80-81	22.879667159063622	26.196597367252934	26.207530762681646	24.716204711001797
82-83	22.85630532876392	26.108611947903448	26.276782658632	24.758300064700624
84-85	22.81048016348313	26.055405647516334	26.290244397092298	24.84386979190824
86-87	22.77144309390496	26.166376017892272	26.188570353679474	24.873610534523287
88-89	22.612833602311454	26.046375341267318	26.166772756670497	25.174018299750728
90-91	22.448670346907093	26.067393111164684	26.249630292290643	25.234306249637584
92-93	22.551214645410486	25.899908961846734	26.355299812012124	25.193576580730653
94-95	22.403394960103835	25.745665620574552	26.434846448816966	25.41609297050464
96-97	22.404168400045382	25.595270130708876	26.463201945650937	25.537359523594798
98-99	22.26430790503652	25.619473072707518	26.36967082087806	25.7465482013779
100-101	22.09475812352599	25.6583818739398	26.40637472796748	25.84048527456673
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	149.0
1	119.5
2	150.0
3	231.0
4	201.5
5	196.5
6	337.5
7	630.5
8	911.5
9	966.0
10	789.0
11	633.0
12	625.5
13	638.0
14	679.5
15	731.5
16	796.0
17	845.5
18	962.0
19	1096.0
20	1209.0
21	1394.0
22	1648.0
23	1983.5
24	2376.5
25	2985.0
26	3868.0
27	5334.0
28	7537.5
29	10616.5
30	14881.5
31	20564.5
32	28798.0
33	34885.0
34	39298.5
35	45891.0
36	53985.0
37	62087.5
38	68732.0
39	75478.5
40	81344.5
41	86531.5
42	92173.5
43	97500.0
44	102536.5
45	109157.0
46	113942.0
47	116198.5
48	118787.5
49	119869.0
50	119056.5
51	117460.0
52	115087.5
53	112800.5
54	109620.5
55	103377.0
56	94079.5
57	83128.0
58	74272.0
59	64998.0
60	53362.0
61	43312.5
62	35457.5
63	26879.5
64	18326.5
65	12752.5
66	9346.0
67	6914.0
68	5046.5
69	3646.0
70	2628.0
71	1809.5
72	1183.0
73	853.0
74	626.0
75	412.5
76	268.0
77	163.0
78	99.5
79	50.5
80	35.5
81	31.0
82	20.5
83	13.5
84	7.0
85	8.0
86	8.0
87	4.5
88	3.0
89	2.0
90	1.5
91	3.5
92	3.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17045004192169333
2	3.272291760442065E-4
3	0.011198509580179511
4	0.005744689979442736
5	0.02548751693410986
6	0.0026541922056918968
7	0.0
8	0.0
9	1.0907639201473549E-4
10-11	0.05255664155243339
12-13	4.908437640663097E-4
14-15	0.005399281404729407
16-17	0.0
18-19	0.0018361192655813807
20-21	3.8176737205157426E-4
22-23	0.0
24-25	8.362523387796388E-4
26-27	0.0025451158136771614
28-29	0.005508357796744142
30-31	0.017015917154298735
32-33	0.01203476191895915
34-35	0.01048951303208373
36-37	0.0044539526739350325
38-39	0.0
40-41	0.0035631621391480264
42-43	0.009544184301289355
44-45	0.007399015258332891
46-47	0.0032541123617729423
48-49	0.008817008354524453
50-51	0.002399680624324181
52-53	0.002436039421662426
54-55	0.004690284856633626
56-57	0.008089832407759548
58-59	0.016234203011526466
60-61	0.007998935414413936
62-63	0.022306122167013408
64-65	0.04204894912168053
66-67	0.06270074600980378
68-69	0.05210215658570533
70-71	0.07042699044418088
72-73	0.03504988063406834
74-75	0.0280144533491179
76-77	0.23580498013718898
78-79	0.08818826294391365
80-81	0.06929986772669527
82-83	0.028323503126492983
84-85	0.053029305917830574
86-87	0.06969981449741598
88-89	0.2980512411802647
90-91	0.30305057581427347
92-93	0.055210833758125286
94-95	0.010525871829421975
96-97	0.015925153234151383
98-99	8.907905347870066E-4
100-101	1.9997338536034839E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2750366.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.23156561307431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.65237451494934	45.99471716093241
2	12.518310397987317	14.829582474064324
3	4.108103725338474	7.299882460581024
4	1.8436092201895595	4.367994419621066
5	1.0166499405382903	3.010888382926092
6	0.6273424772446292	2.22950862616703
7	0.41291831585792704	1.712045882300526
8	0.29388439575393904	1.3925786295806513
9	0.2187950008394954	1.16636134032335
>10	1.232384741039598	12.886159349574624
>50	0.05553845331948162	2.226751340308902
>100	0.018366305486778416	1.837278971283188
>500	9.227739938496562E-4	0.37994144758298454
>1k	7.997374613121436E-4	0.6663095147539144
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.8179398669122583E-5	0.0
12-13	0.0	0.0	0.0	3.6358797338245165E-5	0.0
14-15	1.8179398669122583E-5	0.0	0.0	7.271759467649033E-5	1.8179398669122583E-5
16-17	3.6358797338245165E-5	0.0	0.0	1.9997338536034839E-4	3.6358797338245165E-5
18-19	3.6358797338245165E-5	1.8179398669122583E-5	0.0	3.090497773750839E-4	3.6358797338245165E-5
20-21	3.6358797338245165E-5	3.6358797338245165E-5	0.0	3.999467707206968E-4	3.6358797338245165E-5
22-23	3.6358797338245165E-5	3.6358797338245165E-5	0.0	5.635613587428001E-4	1.0907639201473549E-4
24-25	3.6358797338245165E-5	3.6358797338245165E-5	0.0	8.180729401105161E-4	1.2725579068385807E-4
26-27	3.6358797338245165E-5	3.6358797338245165E-5	0.0	0.0014543518935298066	1.4543518935298066E-4
28-29	3.6358797338245165E-5	3.6358797338245165E-5	0.0	0.0044539526739350325	1.4543518935298066E-4
30-31	3.6358797338245165E-5	7.271759467649033E-5	0.0	0.0173067875330047	1.4543518935298066E-4
32-33	3.6358797338245165E-5	7.271759467649033E-5	0.0	0.0506296252935064	1.4543518935298066E-4
34-35	3.6358797338245165E-5	7.271759467649033E-5	0.0	0.10574956205828606	1.8179398669122582E-4
36-37	3.6358797338245165E-5	7.271759467649033E-5	0.0	0.1985008540681495	1.8179398669122582E-4
38-39	3.6358797338245165E-5	7.271759467649033E-5	0.0	0.3417545155808354	1.8179398669122582E-4
40-41	3.6358797338245165E-5	7.271759467649033E-5	0.0	0.4936433914613546	1.8179398669122582E-4
42-43	3.6358797338245165E-5	7.271759467649033E-5	0.0	0.659675839506451	1.8179398669122582E-4
44-45	3.6358797338245165E-5	7.271759467649033E-5	0.0	0.8662847053810292	1.8179398669122582E-4
46-47	3.6358797338245165E-5	7.271759467649033E-5	0.0	1.1164695898654942	2.1815278402947098E-4
48-49	3.6358797338245165E-5	7.271759467649033E-5	0.0	1.3608916049718474	2.5451158136771614E-4
50-51	3.6358797338245165E-5	7.271759467649033E-5	0.0	1.615621339123593	2.5451158136771614E-4
52-53	3.6358797338245165E-5	7.271759467649033E-5	0.0	1.8724053453249496	2.5451158136771614E-4
54-55	3.6358797338245165E-5	7.271759467649033E-5	0.0	2.1755286387338995	2.5451158136771614E-4
56-57	3.6358797338245165E-5	7.271759467649033E-5	0.0	2.526645544629333	2.5451158136771614E-4
58-59	3.6358797338245165E-5	7.271759467649033E-5	0.0	2.8937603213535943	2.908703787059613E-4
60-61	3.6358797338245165E-5	7.271759467649033E-5	0.0	3.271400969907278	2.908703787059613E-4
62-63	3.6358797338245165E-5	7.271759467649033E-5	0.0	3.650768661334528	2.908703787059613E-4
64-65	3.6358797338245165E-5	7.271759467649033E-5	0.0	4.069749262461796	2.908703787059613E-4
66-67	3.6358797338245165E-5	7.271759467649033E-5	0.0	4.565428746574092	2.908703787059613E-4
68-69	3.6358797338245165E-5	7.271759467649033E-5	0.0	5.088540943278095	2.908703787059613E-4
70-71	3.6358797338245165E-5	7.271759467649033E-5	0.0	5.624287822057138	2.908703787059613E-4
72-73	3.6358797338245165E-5	7.271759467649033E-5	0.0	6.1665429255597255	3.2722917604420645E-4
74-75	3.6358797338245165E-5	9.089699334561291E-5	0.0	6.738266834304962	3.2722917604420645E-4
76-77	3.6358797338245165E-5	1.0907639201473549E-4	0.0	7.385235274141696	3.2722917604420645E-4
78-79	3.6358797338245165E-5	1.0907639201473549E-4	0.0	8.095140792170932	3.2722917604420645E-4
80-81	3.6358797338245165E-5	1.0907639201473549E-4	0.0	8.811754508309075	3.2722917604420645E-4
82-83	3.6358797338245165E-5	1.0907639201473549E-4	0.0	9.547965616212533	3.2722917604420645E-4
84-85	3.6358797338245165E-5	1.0907639201473549E-4	0.0	10.313136506195903	3.2722917604420645E-4
86-87	3.6358797338245165E-5	1.0907639201473549E-4	0.0	11.136954136285862	3.2722917604420645E-4
88-89	3.6358797338245165E-5	1.0907639201473549E-4	0.0	12.023890638555013	3.2722917604420645E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2415	0.0	55.499912	1
GTATCAA	4725	0.0	43.455254	1
TCAACGC	5965	0.0	33.574253	4
CAACGCA	6175	0.0	32.538307	5
ATCAACG	6215	0.0	32.224308	3
TATCAAC	6375	0.0	31.787766	2
AACGCAG	6610	0.0	30.539032	6
ACGCAGA	7705	0.0	25.150547	7
GTACATG	7395	0.0	24.037748	1
CGCAGAG	7965	0.0	23.792881	8
TACATGG	7615	0.0	22.747581	2
ACATGGG	7940	0.0	21.33831	3
GCAGAGT	8905	0.0	20.267939	9
AGAGTAC	7550	0.0	19.564707	10-11
TAAGGTG	1645	0.0	19.346413	5
CATGGGG	5800	0.0	19.064802	4
CAGAGTA	8285	0.0	17.485065	10-11
GAGTACT	4255	0.0	17.396675	12-13
GTAAGGT	1360	0.0	17.09864	4
AGTACTT	4745	0.0	16.800192	12-13
>>END_MODULE