FastQCFastQC Report
Mon 15 Aug 2016
SRR2921679_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921679_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344189
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.040.380431
GTATCAA40600.040.097931
TCAACGC44950.035.055474
CAACGCA46950.033.8639075
ATCAACG46800.033.46793
TATCAAC50650.031.1127572
AACGCAG51150.031.0656326
GTACATG84350.027.5155141
TACATGG86100.026.3514562
ACGCAGA63200.024.6204647
CGCAGAG63350.024.0390558
ACATGGG93000.023.8338573
CATGGGG75550.022.1762264
GAGTACA62800.019.3479181
ATGGGGG49000.018.9840075
GCAGAGT76750.018.0505089
GACGTAT2905.2687028E-818.0031531
CGTACAC5600.017.7988383
GTATAGG11200.017.798571
AGAGTAC63000.017.47158810-11
Produced by FastQC (version 0.11.5)