##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921679_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3344189
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.92861527862211	31.0	31.0	34.0	26.0	34.0
2	29.993931563078522	31.0	31.0	34.0	25.0	34.0
3	30.052699174598086	31.0	31.0	34.0	25.0	34.0
4	33.49541428430032	37.0	35.0	37.0	28.0	37.0
5	33.48762435376709	37.0	35.0	37.0	28.0	37.0
6	33.526637399979485	37.0	35.0	37.0	28.0	37.0
7	33.42017601277918	37.0	35.0	37.0	27.0	37.0
8	33.3877576895325	37.0	35.0	37.0	27.0	37.0
9	34.89631507070922	39.0	35.0	39.0	27.0	39.0
10-11	34.783300226153486	39.0	35.0	39.0	27.0	39.0
12-13	34.66399088089818	39.0	35.0	39.0	27.0	39.0
14-15	35.74711940622973	40.0	36.0	41.0	27.0	41.0
16-17	35.6789248454558	40.0	36.0	41.0	27.0	41.0
18-19	35.645091530412905	40.0	36.0	41.0	26.5	41.0
20-21	35.565357101527454	40.0	36.0	41.0	26.0	41.0
22-23	35.46507972486005	40.0	36.0	41.0	25.0	41.0
24-25	35.3508261644303	40.0	36.0	41.0	25.0	41.0
26-27	35.20272194544029	39.5	35.0	41.0	24.0	41.0
28-29	35.06372516625107	39.0	35.0	41.0	24.0	41.0
30-31	34.91502738033048	39.0	35.0	41.0	23.5	41.0
32-33	34.742013833548285	39.0	35.0	41.0	22.5	41.0
34-35	34.57132222491013	39.0	34.0	41.0	21.5	41.0
36-37	34.53469645405807	39.0	34.5	41.0	21.0	41.0
38-39	34.706734727014535	39.0	35.0	41.0	21.5	41.0
40-41	34.767842218247836	39.5	35.0	41.0	21.5	41.0
42-43	34.617301683606996	39.0	35.0	41.0	19.0	41.0
44-45	34.50086627878987	39.0	35.0	41.0	18.0	41.0
46-47	34.34916612069473	39.0	34.5	41.0	15.5	41.0
48-49	34.18982046170237	39.0	34.0	41.0	14.5	41.0
50-51	32.54645640542445	37.0	32.0	39.5	11.0	40.0
52-53	32.66035756352288	37.5	32.5	39.5	9.0	40.0
54-55	33.564365381262846	38.0	33.0	40.0	8.5	41.0
56-57	33.59624889621968	38.0	33.5	41.0	8.0	41.0
58-59	33.4152392403659	38.0	33.0	40.5	7.0	41.0
60-61	33.17695261840763	38.0	33.0	40.0	4.0	41.0
62-63	32.89773843523796	37.0	33.0	40.0	2.0	41.0
64-65	32.61786549743451	37.0	33.0	40.0	2.0	41.0
66-67	32.29125163081393	36.0	32.0	39.5	2.0	41.0
68-69	31.937500691498	36.0	32.0	39.0	2.0	41.0
70-71	31.533328110343046	35.0	32.0	39.0	2.0	40.5
72-73	31.175136034476523	35.0	31.0	37.5	2.0	40.0
74-75	30.78007597656711	35.0	31.0	37.0	2.0	39.0
76-77	30.407285742522326	35.0	31.0	36.5	2.0	39.0
78-79	30.058755500960025	35.0	31.0	36.0	2.0	39.0
80-81	29.740533504535776	35.0	30.0	36.0	2.0	37.0
82-83	29.425953497245523	35.0	30.0	35.0	2.0	37.0
84-85	29.1607573615008	35.0	30.0	35.0	2.0	36.5
86-87	28.922634904905195	34.0	30.0	35.0	2.0	36.0
88-89	28.699195380404635	34.0	30.0	35.0	2.0	36.0
90-91	28.473856890265473	34.0	29.0	35.0	2.0	35.5
92-93	28.27982808387923	34.0	29.0	35.0	2.0	35.0
94-95	28.04918262693885	34.0	29.0	35.0	2.0	35.0
96-97	27.813723895389884	34.0	29.0	35.0	2.0	35.0
98-99	27.54002809051761	34.0	29.0	35.0	2.0	35.0
100-101	26.47999649541339	33.0	26.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	141080.0
3	23440.0
4	15397.0
5	11673.0
6	10444.0
7	10528.0
8	11404.0
9	12029.0
10	13206.0
11	13880.0
12	13941.0
13	14107.0
14	14519.0
15	15280.0
16	15332.0
17	16053.0
18	15156.0
19	14881.0
20	14960.0
21	15900.0
22	17333.0
23	19888.0
24	23564.0
25	27584.0
26	32559.0
27	38811.0
28	45090.0
29	53177.0
30	62819.0
31	76651.0
32	94423.0
33	121612.0
34	165892.0
35	237287.0
36	386854.0
37	678249.0
38	718020.0
39	131152.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80997800663062	18.16539815597969	13.039934824709764	24.98468901267992
2	15.677906504198855	19.21591464753985	39.976286993218274	25.129891855043024
3	19.095849861510434	23.575604290902298	29.21902418121186	28.109521666375404
4	13.050127956219582	15.502362821117444	36.86195451030712	34.58555471235585
5	14.134880951312272	37.23553297365878	34.628251508867685	14.001334566161267
6	33.85181984158908	35.50088446349775	16.815811784971444	13.831483909941728
7	29.015391719347107	31.48453859832655	21.174096357797513	18.32597332452884
8	26.419324332650035	35.38218410227545	19.682659219200392	18.515832345874113
9	26.603679865405933	15.433939367478464	19.307825014556006	38.6545557525596
10-11	25.650514094995426	25.22208664549277	28.0897737264261	21.03762553308571
12-13	26.015956446906728	22.634786274566398	27.88630541810039	23.46295186042648
14-15	23.14567586498929	24.30271435501031	24.851086730695794	27.700523049304604
16-17	23.57120712312763	27.430026556490457	25.70778049685478	23.290985823527134
18-19	23.595833574449383	26.803746188310363	26.876387538763613	22.72403269847664
20-21	24.00960915549616	26.077814778503104	26.818405863594734	23.094170202405998
22-23	23.868192485278524	25.87536887826606	26.558651831266516	23.6977868051889
24-25	23.720622125329648	26.093466571263107	26.52397911810737	23.661932185299875
26-27	23.5380185610172	26.275303510526076	26.478999742482262	23.707678185974466
28-29	23.680865749701535	26.476426108863492	26.304644678005516	23.538063463429456
30-31	23.86780607422518	26.13211168363272	26.25052541513964	23.749556827002458
32-33	23.568837291228494	26.21900070785461	26.276179331542416	23.93598266937448
34-35	23.967488086889823	26.276635351920824	26.24284353641816	23.513033024771197
36-37	24.026917324080205	26.10459528623878	26.276596317288075	23.591891072392936
38-39	23.85460332687607	26.20701379589586	26.249582092069527	23.68880078515854
40-41	23.98971455005717	26.063953932571334	26.310489553148393	23.635841964223108
42-43	23.827521206691546	26.05549392760726	26.19131686353474	23.925668002166454
44-45	23.695387290429956	26.200482201930637	26.347378055092914	23.756752452546497
46-47	23.949966109089775	26.129262508622553	26.114789202134258	23.80598218015341
48-49	23.740957284218474	26.242605500848082	26.12056255131947	23.89587466361397
50-51	23.875354762966726	26.231506741975874	26.22076440772507	23.672374087332333
52-53	24.034338622364608	26.06494964564734	26.24226457263042	23.65844715935763
54-55	23.5409310628265	25.975956448556854	26.538658738052167	23.944453750564485
56-57	23.68633265737952	26.13720713704142	26.322282831981774	23.854177373597285
58-59	23.696541422876784	25.983443616318908	26.430319724256073	23.88969523654824
60-61	23.65116550551444	26.047828052664475	26.529357437825496	23.771649003995588
62-63	23.68679274966569	26.032415991489582	26.593833694801226	23.686957564043496
64-65	23.482491470445588	25.93447522207707	26.55110832889956	24.031924978577788
66-67	23.517717273363903	26.055708329849843	26.372983431755138	24.05359096503112
68-69	23.69456184857686	25.928305831276937	26.47772271257206	23.89940960757414
70-71	23.56775005696417	25.78868593516137	26.457514918170094	24.18604908970437
72-73	23.528125916271666	25.954357104469917	26.36818790174556	24.149329077512856
74-75	23.49571934906958	26.09163228367255	26.396535866210986	24.016112501046884
76-77	23.57262612223973	25.721456679789895	26.53866338433659	24.167253813633785
78-79	23.49606935390679	25.742150695145373	26.574116473710585	24.187663477237255
80-81	23.618839071776534	25.871331571685495	26.385709162661797	24.124120193876173
82-83	23.544610873203506	25.923498784353406	26.466283789940103	24.065606552502985
84-85	23.481698929178318	25.95510820603652	26.496363162295566	24.066829702489592
86-87	23.46762970562513	25.97228540146149	26.42543729817512	24.134647594738258
88-89	23.35154747792047	25.75095879037298	26.52197761848311	24.37551611322344
90-91	23.36993152732949	25.77178824056637	26.543760084112645	24.314520147991495
92-93	23.38511761732245	25.663352346598856	26.74051261330513	24.211017422773565
94-95	23.308101643169795	25.491740686868503	26.795425472841274	24.404732197120424
96-97	23.316487920226006	25.34723054933354	26.822563180791313	24.513718349649142
98-99	23.202187618731447	25.336060113052945	26.783476554215728	24.678275713999874
100-101	23.369169481307654	25.18837135334494	26.911570160478753	24.53088900486865
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	17.0
2	30.0
3	45.5
4	49.0
5	59.5
6	97.5
7	184.0
8	304.0
9	370.5
10	364.0
11	370.5
12	459.5
13	569.5
14	645.0
15	749.0
16	841.0
17	941.0
18	1026.0
19	1114.0
20	1230.5
21	1346.5
22	1494.5
23	1758.0
24	2137.0
25	2660.0
26	3552.5
27	5073.0
28	7502.5
29	11104.5
30	16181.0
31	22929.0
32	32049.5
33	39218.5
34	44798.5
35	52531.5
36	62790.5
37	73428.0
38	82492.5
39	90862.5
40	98362.5
41	105057.5
42	111815.0
43	119361.5
44	126654.5
45	134785.5
46	139107.0
47	141518.5
48	144749.5
49	145283.0
50	143600.5
51	140804.5
52	138439.0
53	136421.0
54	133541.5
55	126270.5
56	115026.5
57	103323.5
58	93163.5
59	81700.0
60	67157.0
61	55014.0
62	45372.0
63	34675.5
64	25040.0
65	18407.5
66	13802.5
67	10634.5
68	8079.5
69	6055.5
70	4679.0
71	3467.0
72	2461.0
73	1776.0
74	1363.0
75	978.0
76	704.0
77	500.5
78	319.0
79	233.0
80	193.0
81	149.0
82	121.5
83	104.0
84	78.5
85	70.5
86	64.5
87	49.0
88	46.0
89	39.5
90	29.0
91	28.0
92	26.5
93	22.0
94	15.0
95	11.0
96	17.5
97	15.5
98	5.5
99	4.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05436295616067155
2	0.07664040519240987
3	0.0723942336991121
4	0.0723942336991121
5	0.06814806220581432
6	0.010585526117094459
7	0.013246858954443065
8	0.01767244614464075
9	0.006219744159196744
10-11	0.013426274651342973
12-13	0.02318947882431286
14-15	0.04148987990810328
16-17	0.028063605256760306
18-19	0.01086960097051931
20-21	0.021260760082638868
22-23	0.0383949591365799
24-25	0.035793431531531264
26-27	0.021828909789488572
28-29	0.01211055954074366
30-31	0.012095608232668668
32-33	0.008432537754295586
34-35	0.005502081371597119
36-37	6.877601714496399E-4
38-39	0.004515295038647636
40-41	0.0014203742671242564
42-43	0.23503456293887695
44-45	0.2030537149664687
46-47	0.20964724182754024
48-49	0.21676406447123653
50-51	0.20709056814671659
52-53	0.2149549561941625
54-55	0.2116507171095892
56-57	0.21458117349228764
58-59	0.2233725426403831
60-61	0.22823171776475554
62-63	0.21235342858911382
64-65	0.2127720652152136
66-67	0.20997617060519008
68-69	0.20430962484476803
70-71	0.19629572371657222
72-73	0.20798764663121613
74-75	0.20646261320756693
76-77	0.20102033706826974
78-79	0.2192758842278352
80-81	0.231132271531304
82-83	0.23004082604182957
84-85	0.22423971850873262
86-87	0.24584435867709628
88-89	0.23784540885697547
90-91	0.24479776711184686
92-93	0.23950500405329964
94-95	0.2424055578198481
96-97	0.25013538409461905
98-99	0.24345214938509754
100-101	0.24992606578156917
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3344189.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.83900386470806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.01397461212505	45.90264553704063
2	12.011416437970567	14.134795564287364
3	4.111520936915984	7.257533888910832
4	1.8578194374367707	4.372489802370876
5	1.0541449401094238	3.1012419102530413
6	0.6486820423337389	2.290068311750495
7	0.4311293633409137	1.77570555930636
8	0.3178580290050158	1.496195983764369
9	0.2464851024459772	1.3052644105870632
>10	1.2208517277181674	12.630880521330296
>50	0.0635944095719798	2.531013406216955
>100	0.020530365989909795	2.052709502764018
>500	0.0011240279721014658	0.4511423856151633
>1k	8.685670643494284E-4	0.6983132158025606
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.980523229996869E-5	0.0
3	0.0	0.0	0.0	5.980523229996869E-5	0.0
4	0.0	0.0	0.0	8.970784844995304E-5	0.0
5	0.0	0.0	0.0	1.1961046459993738E-4	0.0
6	0.0	0.0	0.0	1.1961046459993738E-4	0.0
7	0.0	0.0	0.0	1.1961046459993738E-4	0.0
8	0.0	0.0	0.0	1.1961046459993738E-4	0.0
9	0.0	0.0	0.0	1.4951308074992174E-4	0.0
10-11	0.0	0.0	0.0	1.7941569689990607E-4	0.0
12-13	0.0	1.4951308074992173E-5	0.0	2.0931831304989043E-4	5.980523229996869E-5
14-15	0.0	2.9902616149984345E-5	0.0	2.0931831304989043E-4	1.4951308074992174E-4
16-17	0.0	2.9902616149984345E-5	0.0	2.0931831304989043E-4	1.4951308074992174E-4
18-19	0.0	2.9902616149984345E-5	0.0	2.0931831304989043E-4	2.0931831304989043E-4
20-21	0.0	2.9902616149984345E-5	0.0	2.0931831304989043E-4	2.990261614998435E-4
22-23	0.0	2.9902616149984345E-5	0.0	2.3922092919987476E-4	3.289287776498278E-4
24-25	0.0	4.485392422497652E-5	0.0	2.990261614998435E-4	3.5883139379981214E-4
26-27	0.0	5.980523229996869E-5	0.0	4.485392422497652E-4	3.887340099497965E-4
28-29	0.0	5.980523229996869E-5	0.0	0.0014502768832742408	3.887340099497965E-4
30-31	0.0	5.980523229996869E-5	0.0	0.007027114795246321	4.1863662609978086E-4
32-33	0.0	5.980523229996869E-5	0.0	0.021649494092588668	4.485392422497652E-4
34-35	0.0	5.980523229996869E-5	0.0	0.04214773746340293	4.784418583997495E-4
36-37	0.0	7.475654037496086E-5	1.4951308074992173E-5	0.07865883178253383	4.784418583997495E-4
38-39	0.0	8.970784844995304E-5	2.9902616149984345E-5	0.14149917962172592	4.933931664747417E-4
40-41	0.0	8.970784844995304E-5	2.9902616149984345E-5	0.21150120402883926	5.083444745497339E-4
42-43	0.0	8.970784844995304E-5	2.9902616149984345E-5	0.28374592464720144	5.083444745497339E-4
44-45	0.0	8.970784844995304E-5	2.9902616149984345E-5	0.37180912920890535	5.083444745497339E-4
46-47	0.0	8.970784844995304E-5	2.9902616149984345E-5	0.4766477014307505	5.232957826247261E-4
48-49	0.0	8.970784844995304E-5	2.9902616149984345E-5	0.5928941217138146	5.681497068497026E-4
50-51	0.0	8.970784844995304E-5	2.9902616149984345E-5	0.7185000608518239	5.681497068497026E-4
52-53	0.0	8.970784844995304E-5	2.9902616149984345E-5	0.8462589883526319	5.831010149246948E-4
54-55	0.0	8.970784844995304E-5	2.9902616149984345E-5	0.9878329245147328	5.98052322999687E-4
56-57	0.0	8.970784844995304E-5	2.9902616149984345E-5	1.1498153961991981	6.13003631074679E-4
58-59	0.0	8.970784844995304E-5	2.9902616149984345E-5	1.3262258801760307	6.429062472246634E-4
60-61	0.0	8.970784844995304E-5	2.9902616149984345E-5	1.5209218139285787	6.578575552996556E-4
62-63	0.0	8.970784844995304E-5	2.9902616149984345E-5	1.7202526531843745	6.578575552996556E-4
64-65	0.0	8.970784844995304E-5	2.9902616149984345E-5	1.9322023964554633	6.578575552996556E-4
66-67	0.0	8.970784844995304E-5	2.9902616149984345E-5	2.1763722086281607	6.578575552996556E-4
68-69	0.0	8.970784844995304E-5	2.9902616149984345E-5	2.4456153644426197	6.578575552996556E-4
70-71	0.0	8.970784844995304E-5	2.9902616149984345E-5	2.731708644457595	7.176627875996243E-4
72-73	0.0	8.970784844995304E-5	2.9902616149984345E-5	3.033082161325212	7.77468019899593E-4
74-75	0.0	8.970784844995304E-5	2.9902616149984345E-5	3.345534597476399	8.073706360495773E-4
76-77	0.0	8.970784844995304E-5	2.9902616149984345E-5	3.689444585817369	8.522245602745539E-4
78-79	0.0	8.970784844995304E-5	2.9902616149984345E-5	4.0827237934219625	8.970784844995304E-4
80-81	0.0	8.970784844995304E-5	2.9902616149984345E-5	4.496695611402346	9.120297925745226E-4
82-83	0.0	8.970784844995304E-5	2.9902616149984345E-5	4.927024160416771	9.56883716799499E-4
84-85	0.0	8.970784844995304E-5	2.9902616149984345E-5	5.3759072827522605	9.56883716799499E-4
86-87	0.0	8.970784844995304E-5	2.9902616149984345E-5	5.8520167370923115	9.867863329494834E-4
88-89	0.0	8.970784844995304E-5	2.9902616149984345E-5	6.363904671655819	0.00103164025717446
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2245	0.0	40.38043	1
GTATCAA	4060	0.0	40.09793	1
TCAACGC	4495	0.0	35.05547	4
CAACGCA	4695	0.0	33.863907	5
ATCAACG	4680	0.0	33.4679	3
TATCAAC	5065	0.0	31.112757	2
AACGCAG	5115	0.0	31.065632	6
GTACATG	8435	0.0	27.515514	1
TACATGG	8610	0.0	26.351456	2
ACGCAGA	6320	0.0	24.620464	7
CGCAGAG	6335	0.0	24.039055	8
ACATGGG	9300	0.0	23.833857	3
CATGGGG	7555	0.0	22.176226	4
GAGTACA	6280	0.0	19.347918	1
ATGGGGG	4900	0.0	18.984007	5
GCAGAGT	7675	0.0	18.050508	9
GACGTAT	290	5.2687028E-8	18.003153	1
CGTACAC	560	0.0	17.798838	3
GTATAGG	1120	0.0	17.79857	1
AGAGTAC	6300	0.0	17.471588	10-11
>>END_MODULE