FastQCFastQC Report
Mon 15 Aug 2016
SRR2921679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344189
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22900.043.801481
GTATCAA40750.039.1970561
TCAACGC46900.032.3849264
CAACGCA49500.030.807435
ATCAACG50350.030.1668033
TATCAAC51400.029.5505542
AACGCAG53550.028.4727736
GTACATG84550.024.7389281
TACATGG88100.023.4903452
ACATGGG90650.022.4106663
ACGCAGA67250.021.9657367
CGCAGAG67500.021.321448
CCCTATA6450.019.8693122
CATGGGG75200.018.9982874
AGAGTAC67350.017.03243410-11
TAAGGTG18750.016.9757585
GCAGAGT79650.016.9367379
CGTACAC5300.016.1203843
CCTATAC11550.016.0273383
GAGTACA64200.015.9941271
Produced by FastQC (version 0.11.5)