##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921679_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3344189
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.534289778478428	33.0	31.0	34.0	30.0	34.0
2	31.664900817507622	34.0	31.0	34.0	30.0	34.0
3	31.738616746840563	34.0	31.0	34.0	29.0	34.0
4	35.22796319227173	37.0	35.0	37.0	33.0	37.0
5	34.99669845215088	37.0	35.0	37.0	32.0	37.0
6	34.98254823516255	37.0	35.0	37.0	32.0	37.0
7	34.87482764879616	37.0	35.0	37.0	32.0	37.0
8	34.824745551163524	37.0	35.0	37.0	32.0	37.0
9	36.431790487918	39.0	37.0	39.0	32.0	39.0
10-11	36.32553767146534	39.0	37.0	39.0	32.0	39.0
12-13	36.25221406445628	39.0	37.0	39.0	32.0	39.0
14-15	37.51194146024642	40.0	38.0	41.0	32.0	41.0
16-17	37.481914748239404	40.0	38.0	41.0	32.0	41.0
18-19	37.44471395007878	40.0	38.0	41.0	32.0	41.0
20-21	37.39260116578339	40.0	38.0	41.0	32.0	41.0
22-23	37.309328659355074	40.0	38.0	41.0	31.0	41.0
24-25	37.22461215559288	40.0	38.0	41.0	31.0	41.0
26-27	37.10415589549514	40.0	37.0	41.0	31.0	41.0
28-29	36.99047272746846	40.0	37.0	41.0	30.5	41.0
30-31	36.84043515483126	40.0	37.0	41.0	30.0	41.0
32-33	36.79891492376777	40.0	37.0	41.0	30.0	41.0
34-35	36.93083554787125	40.0	37.0	41.0	30.0	41.0
36-37	36.93398474189108	40.0	37.0	41.0	30.0	41.0
38-39	36.83926626156596	40.0	37.0	41.0	30.0	41.0
40-41	36.76081226270405	40.0	37.0	41.0	30.0	41.0
42-43	36.64634011415025	40.0	37.0	41.0	30.0	41.0
44-45	36.52585993195959	40.0	37.0	41.0	30.0	41.0
46-47	36.377640587897396	40.0	36.0	41.0	29.5	41.0
48-49	36.246330874241856	40.0	36.0	41.0	29.0	41.0
50-51	36.10351239119559	40.0	36.0	41.0	29.0	41.0
52-53	35.9387456869214	39.5	35.0	41.0	28.0	41.0
54-55	35.73619403688009	39.0	35.0	41.0	28.0	41.0
56-57	35.51948185344787	39.0	35.0	41.0	28.0	41.0
58-59	35.28121242549389	39.0	35.0	41.0	27.5	41.0
60-61	35.014116875571325	38.0	35.0	40.0	27.0	41.0
62-63	34.71934809904583	38.0	34.5	40.0	26.0	41.0
64-65	34.38794383331803	37.0	34.0	40.0	26.0	41.0
66-67	34.04030842754402	37.0	34.0	39.5	26.0	41.0
68-69	33.682379046160364	36.0	34.0	39.0	26.0	41.0
70-71	33.286131256337484	35.5	34.0	39.0	25.0	40.0
72-73	32.919829889997246	35.0	33.0	38.0	24.5	40.0
74-75	32.51495355077121	35.0	33.0	37.0	23.5	39.0
76-77	29.78308896417039	33.0	29.0	34.5	19.0	36.5
78-79	31.358476898285353	34.5	31.5	36.0	20.5	38.0
80-81	31.53642168549684	35.0	32.0	36.0	22.0	37.0
82-83	31.34978435728363	35.0	32.0	35.0	21.0	37.0
84-85	31.144333648606583	35.0	32.0	35.0	20.0	36.5
86-87	30.92304292610256	35.0	32.0	35.0	20.0	36.0
88-89	30.64595317429727	35.0	32.0	35.0	18.0	36.0
90-91	30.444232966498006	35.0	32.0	35.0	16.0	35.5
92-93	30.281171159883606	34.0	32.0	35.0	10.5	35.0
94-95	30.17574186147972	34.0	32.0	35.0	4.5	35.0
96-97	29.972743615866207	34.0	31.0	35.0	2.0	35.0
98-99	29.772456939485178	34.0	31.0	35.0	2.0	35.0
100-101	28.65025900150978	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48354.0
3	17410.0
4	10082.0
5	7210.0
6	6402.0
7	6304.0
8	6762.0
9	7254.0
10	8216.0
11	9151.0
12	9788.0
13	10328.0
14	11111.0
15	11914.0
16	12816.0
17	13529.0
18	12768.0
19	11924.0
20	11627.0
21	11614.0
22	12584.0
23	14047.0
24	16650.0
25	20198.0
26	24284.0
27	29451.0
28	35427.0
29	43309.0
30	52815.0
31	65210.0
32	82847.0
33	110379.0
34	155741.0
35	232505.0
36	407752.0
37	798558.0
38	878142.0
39	119718.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.70750790793993	19.668449079336384	13.638377192785406	25.98566581993828
2	14.859747465897492	20.59239000748165	38.513663430997475	26.034199095623382
3	17.693004728111177	24.682086110836973	29.166943295562277	28.45796586548957
4	12.761718045615309	16.821635348469457	35.78587856671439	34.63076803920085
5	13.892064335240995	37.506168940126734	34.714338441365314	13.887428283266964
6	33.696369854083656	36.171768375901735	16.62790196967323	13.503959800341375
7	28.776244404846736	31.68777243152226	21.214829664232493	18.32115349939851
8	26.30877022799848	36.05056412780498	19.39334170407235	18.247323940124197
9	26.26615899998475	15.611049495109278	19.190183329949352	38.93260817495662
10-11	25.58754344618059	25.53421505924836	28.0517636886789	20.826477805892154
12-13	25.965442922811366	22.7757647433487	27.935822784526238	23.322969549313697
14-15	22.976680066177686	24.4913506243594	24.856150255941376	27.67581905352154
16-17	23.495547051916024	27.6973580141553	25.645290980862622	23.161803953066048
18-19	23.53583302794734	27.004688964553846	26.81093145792693	22.648546549571886
20-21	23.929536110635162	26.181178590512893	26.794542520276266	23.09474277857568
22-23	23.786379896590773	25.98426703753885	26.55841221892662	23.670940846943758
24-25	23.64900273416247	26.215589914204386	26.53701510554866	23.59839224608449
26-27	23.478223165178594	26.398223135275117	26.49955107402083	23.62400262552546
28-29	23.597542467789374	26.611892027673406	26.304207081030086	23.48635842350714
30-31	23.786125966133042	26.346679475166535	26.244275279465658	23.622919279234768
32-33	23.511236723712205	26.374843160778166	26.21379838240464	23.90012173310499
34-35	23.87538249346262	26.392572270031412	26.310615793540855	23.42142944296511
36-37	23.91848695004431	26.238171727814763	26.31717764084815	23.52616368129278
38-39	23.809629180647384	26.322420772270945	26.235733088052143	23.632216959029527
40-41	23.87550356026433	26.170899565752865	26.294058112233813	23.65953876174899
42-43	23.75656067852903	26.208307982297814	26.16531900586758	23.86981233330557
44-45	23.6740074905471	26.255835916108744	26.349841564521938	23.72031502882222
46-47	23.89656359798084	26.236949077985788	26.121341260014574	23.7451460640188
48-49	23.637065053968033	26.34607577580219	26.165701345219734	23.851157825010034
50-51	23.766308990993366	26.336095518931753	26.169039658310734	23.728555831764147
52-53	23.9899076361815	26.165746517200006	26.167421102011375	23.676924744607128
54-55	23.430862496514322	26.11599583438309	26.49822781857587	23.954913850526722
56-57	23.61309668261653	26.222672193720204	26.2362640443516	23.927967079311664
58-59	23.581342447514007	26.165117563001434	26.336397246844324	23.917142742640237
60-61	23.56310667707756	26.21256167512926	26.390914530883247	23.83341711690994
62-63	23.617017999295037	26.18219308187848	26.41431951825614	23.786469400570343
64-65	23.36290802309874	26.061680107826433	26.398226386155592	24.17718548291923
66-67	23.3653996909757	26.26504578514553	26.259615119929435	24.109939403949333
68-69	23.586784704929027	26.139668039451596	26.280372137357478	23.9931751182619
70-71	23.46325426783006	25.990221406051035	26.27208274116619	24.274441584952715
72-73	23.441386139828158	26.139911914724767	26.161763476363596	24.25693846908348
74-75	23.387839603595836	26.257513449843557	26.16235168456365	24.192295261996954
76-77	23.4297426779572	25.952309160511263	26.373800940185184	24.244147221346353
78-79	23.38575592213841	25.946615122468096	26.354924424770367	24.312704530623122
80-81	23.519836429333502	26.113480591019623	26.115964193631836	24.250718786015042
82-83	23.45680526363175	26.20202898802151	26.161185411888994	24.179980336457746
84-85	23.41587028049099	26.171669370527688	26.170741905058954	24.241718443922373
86-87	23.402780479192785	26.27934360103774	26.07260306355424	24.245272856215234
88-89	23.296057022812814	26.084604773285225	26.10228494041154	24.517053263490425
90-91	23.194819634555625	26.136796668196837	26.168469853710974	24.499913843536564
92-93	23.279809634564963	26.048153527383732	26.299468931656723	24.372567906394586
94-95	23.122596813420387	25.875322512031353	26.375820812278	24.62625986227026
96-97	23.105744315857223	25.815416988666406	26.34475764485203	24.73408105062434
98-99	22.985152329687473	25.808805257880824	26.320832041280305	24.885210371151388
100-101	22.99882646919732	25.782691519584088	26.31878682421992	24.89969518699867
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	19.0
2	24.0
3	36.5
4	32.5
5	37.5
6	60.0
7	134.5
8	217.5
9	244.5
10	228.0
11	204.0
12	233.0
13	257.5
14	276.0
15	332.5
16	381.5
17	431.0
18	495.5
19	560.5
20	622.0
21	766.5
22	952.0
23	1192.5
24	1621.5
25	2188.5
26	3178.5
27	4922.5
28	7489.0
29	11366.0
30	16597.5
31	23495.5
32	32884.5
33	40097.0
34	45704.5
35	53378.0
36	63406.5
37	74111.5
38	83614.0
39	91974.5
40	98891.0
41	105542.5
42	112509.5
43	120322.5
44	127445.0
45	135963.5
46	140858.5
47	142706.5
48	145903.0
49	146512.5
50	144976.5
51	141856.0
52	139657.5
53	138349.0
54	134744.0
55	126630.5
56	115300.0
57	102810.0
58	91854.0
59	80545.5
60	65990.5
61	53517.0
62	43952.5
63	33410.5
64	23783.5
65	17173.0
66	12781.5
67	9872.5
68	7338.0
69	5454.0
70	4185.0
71	3018.0
72	2052.5
73	1433.0
74	1025.5
75	721.5
76	497.0
77	310.0
78	182.5
79	102.0
80	65.5
81	47.0
82	32.0
83	18.5
84	19.0
85	12.0
86	7.0
87	7.5
88	6.0
89	3.5
90	3.0
91	3.0
92	1.5
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1631486737143146
2	2.0931831304989043E-4
3	0.01073503919784438
4	0.0053824709069971825
5	0.024729463556037057
6	0.0024221119081487322
7	0.0
8	0.0
9	0.0
10-11	0.05058027521769851
12-13	3.4388008572481995E-4
14-15	0.004829272508222472
16-17	0.0
18-19	0.0018091082770740527
20-21	2.3922092919987476E-4
22-23	0.0
24-25	6.130036310746792E-4
26-27	0.0028856024584734893
28-29	0.005502081371597119
30-31	0.017223906902390982
32-33	0.011916192535768762
34-35	0.010196792107144663
36-37	0.004051804488322879
38-39	0.0
40-41	0.0037527783268230352
42-43	0.00922495708227017
44-45	0.0072065304921462275
46-47	0.003722875710673051
48-49	0.009314664930720123
50-51	0.002930456382698466
52-53	0.0022875501354738025
54-55	0.004380733265972707
56-57	0.00817836551702072
58-59	0.015997899640241623
60-61	0.007655069734395992
62-63	0.022501718652863222
64-65	0.04214773746340294
66-67	0.0615096814205178
68-69	0.05123813277299817
70-71	0.06870126060458903
72-73	0.03524023313275655
74-75	0.027943994792160372
76-77	0.2339879713736275
78-79	0.08520750471938039
80-81	0.06786398735238947
82-83	0.028287874877885193
84-85	0.05222491910594766
86-87	0.06934416685181369
88-89	0.2973665663035194
90-91	0.3031676738366163
92-93	0.053092094974297205
94-95	0.009613691092219967
96-97	0.014981210691142156
98-99	8.372732521995617E-4
100-101	2.2426962112488262E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3344189.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.82697701329538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.2312856536369	43.31993517533178
2	13.031613246926316	14.810943728584745
3	4.38977805295979	7.483734495270436
4	1.9983055722151346	4.5423065927123805
5	1.1188724280777447	3.1791068875591995
6	0.7100527439555239	2.4210090575392718
7	0.4823642987097825	1.918791344037061
8	0.3498918915805238	1.5906638783987892
9	0.2583210248978871	1.3211642649530821
>10	1.3347443352273642	13.385713225865608
>50	0.07028030468060734	2.6923917836772575
>100	0.022326678378499012	2.1365149377833808
>500	0.0013193711936404172	0.5286099190465449
>1k	8.443975602275319E-4	0.6691147092404091
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9902616149984345E-5
2	0.0	0.0	0.0	0.0	2.9902616149984345E-5
3	0.0	0.0	0.0	0.0	2.9902616149984345E-5
4	0.0	0.0	0.0	0.0	2.9902616149984345E-5
5	0.0	0.0	0.0	2.9902616149984345E-5	2.9902616149984345E-5
6	0.0	0.0	0.0	2.9902616149984345E-5	2.9902616149984345E-5
7	0.0	0.0	0.0	2.9902616149984345E-5	2.9902616149984345E-5
8	0.0	0.0	0.0	2.9902616149984345E-5	2.9902616149984345E-5
9	0.0	0.0	0.0	5.980523229996869E-5	2.9902616149984345E-5
10-11	0.0	0.0	0.0	8.970784844995304E-5	2.9902616149984345E-5
12-13	0.0	0.0	0.0	1.7941569689990607E-4	7.475654037496086E-5
14-15	0.0	0.0	0.0	1.7941569689990607E-4	1.495130807499217E-4
16-17	0.0	0.0	0.0	1.9436700497489824E-4	1.7941569689990607E-4
18-19	0.0	0.0	0.0	2.0931831304989043E-4	2.990261614998435E-4
20-21	0.0	0.0	0.0	2.0931831304989043E-4	3.289287776498278E-4
22-23	0.0	0.0	0.0	2.691235453498591E-4	4.3358793417477305E-4
24-25	0.0	0.0	0.0	3.4388008572482E-4	4.784418583997495E-4
26-27	0.0	0.0	0.0	5.083444745497339E-4	5.382470906997182E-4
28-29	0.0	0.0	0.0	0.001554936039799186	5.382470906997182E-4
30-31	0.0	0.0	0.0	0.007311189648671173	5.531983987747104E-4
32-33	0.0	0.0	0.0	0.02256152388516319	5.681497068497026E-4
34-35	0.0	0.0	0.0	0.043553160422452206	5.681497068497026E-4
36-37	0.0	0.0	0.0	0.08067725837265777	5.98052322999687E-4
38-39	0.0	0.0	0.0	0.145192152716249	6.279549391496712E-4
40-41	0.0	0.0	0.0	0.2162856226128368	6.429062472246634E-4
42-43	0.0	2.9902616149984345E-5	0.0	0.28938256779147353	6.578575552996556E-4
44-45	0.0	2.9902616149984345E-5	0.0	0.37909041624142653	7.027114795246322E-4
46-47	0.0	2.9902616149984345E-5	0.0	0.4854091679626959	7.475654037496087E-4
48-49	0.0	2.9902616149984345E-5	0.0	0.6026573258867846	7.475654037496087E-4
50-51	1.4951308074992173E-5	2.9902616149984345E-5	0.0	0.7293397592061932	7.77468019899593E-4
52-53	2.9902616149984345E-5	2.9902616149984345E-5	0.0	0.8592965289940252	8.372732521995617E-4
54-55	2.9902616149984345E-5	2.9902616149984345E-5	0.0	1.0021114237263504	8.67175868349546E-4
56-57	2.9902616149984345E-5	2.9902616149984345E-5	0.0	1.1649012660468652	8.821271764245382E-4
58-59	2.9902616149984345E-5	2.9902616149984345E-5	0.0	1.342941442603872	8.970784844995304E-4
60-61	2.9902616149984345E-5	2.9902616149984345E-5	0.0	1.539401630709269	8.970784844995304E-4
62-63	2.9902616149984345E-5	5.980523229996869E-5	0.0	1.7411545818732135	9.867863329494834E-4
64-65	2.9902616149984345E-5	8.970784844995304E-5	0.0	1.9570215678599503	0.0010166889490994678
66-67	2.9902616149984345E-5	8.970784844995304E-5	0.0	2.2037929076376965	0.00103164025717446
68-69	2.9902616149984345E-5	8.970784844995304E-5	0.0	2.4750993439665043	0.0010465915652494522
70-71	2.9902616149984345E-5	8.970784844995304E-5	0.0	2.765573357247452	0.0010764941813994364
72-73	2.9902616149984345E-5	8.970784844995304E-5	0.0	3.0712378995325924	0.0011961046459993737
74-75	2.9902616149984345E-5	8.970784844995304E-5	0.0	3.3879664097932265	0.001226007262149358
76-77	2.9902616149984345E-5	8.970784844995304E-5	0.0	3.736720621950494	0.0012409585702243503
78-79	2.9902616149984345E-5	8.970784844995304E-5	0.0	4.1365335511838595	0.0012858124944493269
80-81	2.9902616149984345E-5	8.970784844995304E-5	0.0	4.55741287349489	0.0013306664186743034
82-83	2.9902616149984345E-5	8.970784844995304E-5	0.0	4.995770274945585	0.00137552034289928
84-85	2.9902616149984345E-5	8.970784844995304E-5	0.0	5.451396437222896	0.0013904716509742722
86-87	2.9902616149984345E-5	8.970784844995304E-5	0.0	5.9359533806253175	0.0014502768832742408
88-89	2.9902616149984345E-5	8.970784844995304E-5	0.0	6.459697702492294	0.0014951308074992173
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2290	0.0	43.80148	1
GTATCAA	4075	0.0	39.197056	1
TCAACGC	4690	0.0	32.384926	4
CAACGCA	4950	0.0	30.80743	5
ATCAACG	5035	0.0	30.166803	3
TATCAAC	5140	0.0	29.550554	2
AACGCAG	5355	0.0	28.472773	6
GTACATG	8455	0.0	24.738928	1
TACATGG	8810	0.0	23.490345	2
ACATGGG	9065	0.0	22.410666	3
ACGCAGA	6725	0.0	21.965736	7
CGCAGAG	6750	0.0	21.32144	8
CCCTATA	645	0.0	19.869312	2
CATGGGG	7520	0.0	18.998287	4
AGAGTAC	6735	0.0	17.032434	10-11
TAAGGTG	1875	0.0	16.975758	5
GCAGAGT	7965	0.0	16.936737	9
CGTACAC	530	0.0	16.120384	3
CCTATAC	1155	0.0	16.027338	3
GAGTACA	6420	0.0	15.994127	1
>>END_MODULE