FastQCFastQC Report
Mon 15 Aug 2016
SRR2921678_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921678_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671971
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19850.049.0147821
GTATCAA37850.045.6424831
TCAACGC46700.036.5904854
CAACGCA47600.035.7949075
ATCAACG48100.035.424813
TATCAAC50050.033.8549582
AACGCAG50650.033.71426
ACGCAGA59150.028.2310127
GTACATG61950.027.1203731
CGCAGAG61600.026.8006428
TACATGG66800.024.8685072
ACATGGG67900.023.6967123
CATGGGG58150.021.2229714
GCAGAGT71600.021.0702139
AGAGTAC57100.019.64942610-11
GTATAAT8600.018.7635151
CAGAGTA64350.017.54620610-11
GAGTACT34150.017.22605312-13
GAGTACA48850.016.807971
GTATAGC9100.016.6894631
Produced by FastQC (version 0.11.5)