##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921678_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2671971
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.978317504194468	31.0	31.0	34.0	26.0	34.0
2	30.0407942301769	31.0	31.0	34.0	26.0	34.0
3	30.092594193574705	31.0	31.0	34.0	26.0	34.0
4	33.54887609184381	37.0	35.0	37.0	28.0	37.0
5	33.53388902798721	37.0	35.0	37.0	28.0	37.0
6	33.574234151493414	37.0	35.0	37.0	28.0	37.0
7	33.47164471470686	37.0	35.0	37.0	28.0	37.0
8	33.44095950143171	37.0	35.0	37.0	27.0	37.0
9	34.95295682475596	39.0	35.0	39.0	28.0	39.0
10-11	34.84062738704874	39.0	35.0	39.0	27.0	39.0
12-13	34.725249263558624	39.0	35.0	39.0	27.0	39.0
14-15	35.80468893562093	40.0	36.0	41.0	27.0	41.0
16-17	35.73545408988346	40.0	36.0	41.0	27.0	41.0
18-19	35.70713024205727	40.0	36.0	41.0	27.0	41.0
20-21	35.628359926062075	40.0	36.0	41.0	26.0	41.0
22-23	35.51814128970712	40.0	36.0	41.0	25.5	41.0
24-25	35.40334644350556	40.0	36.0	41.0	25.0	41.0
26-27	35.24825419138156	39.5	35.0	41.0	24.5	41.0
28-29	35.10175391125128	39.0	35.0	41.0	24.0	41.0
30-31	34.94271157883075	39.0	35.0	41.0	24.0	41.0
32-33	34.75312868290861	39.0	35.0	41.0	23.0	41.0
34-35	34.567931313625785	39.0	34.0	41.0	21.5	41.0
36-37	34.521620556510534	39.0	34.5	41.0	21.0	41.0
38-39	34.692834241090196	39.0	35.0	41.0	21.5	41.0
40-41	34.74180277405705	39.0	35.0	41.0	21.0	41.0
42-43	34.57698361995695	39.0	35.0	41.0	18.5	41.0
44-45	34.44361840005	39.0	34.5	41.0	17.0	41.0
46-47	34.268376790017555	39.0	34.0	41.0	15.0	41.0
48-49	34.095477084145	39.0	34.0	41.0	13.5	41.0
50-51	32.42367282429338	37.0	32.0	39.5	8.5	40.0
52-53	32.53805842204126	37.5	32.0	39.5	8.0	40.0
54-55	33.442066175119415	38.0	33.0	40.0	8.0	41.0
56-57	33.467116222444034	38.0	33.0	41.0	7.0	41.0
58-59	33.2782964336065	38.0	33.0	40.0	2.0	41.0
60-61	33.033538537656284	37.5	33.0	40.0	2.0	41.0
62-63	32.74773491179357	37.0	33.0	40.0	2.0	41.0
64-65	32.45542990548924	36.5	32.0	40.0	2.0	41.0
66-67	32.11637083636012	36.0	32.0	39.0	2.0	41.0
68-69	31.75087267039949	35.5	32.0	39.0	2.0	41.0
70-71	31.334820999180007	35.0	31.0	39.0	2.0	40.5
72-73	30.972994280252294	35.0	31.0	37.5	2.0	40.0
74-75	30.565277093201985	35.0	31.0	37.0	2.0	39.0
76-77	30.181239242491777	35.0	31.0	36.5	2.0	39.0
78-79	29.82583680736056	35.0	30.0	36.0	2.0	38.5
80-81	29.501834975005345	35.0	30.0	36.0	2.0	37.0
82-83	29.177134594649416	35.0	30.0	35.0	2.0	37.0
84-85	28.902939440585246	34.5	30.0	35.0	2.0	36.5
86-87	28.65421293868833	34.0	29.0	35.0	2.0	36.0
88-89	28.41567816417169	34.0	29.0	35.0	2.0	36.0
90-91	28.17434470658551	34.0	29.0	35.0	2.0	35.5
92-93	27.964946288713463	34.0	29.0	35.0	2.0	35.0
94-95	27.713497451881025	34.0	29.0	35.0	2.0	35.0
96-97	27.450436774950028	34.0	28.5	35.0	2.0	35.0
98-99	27.150740221357193	34.0	27.0	35.0	2.0	35.0
100-101	26.06276565127391	33.0	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	109064.0
3	18184.0
4	12285.0
5	9374.0
6	8424.0
7	8694.0
8	9279.0
9	9940.0
10	10991.0
11	11659.0
12	11631.0
13	11777.0
14	12636.0
15	13183.0
16	13819.0
17	14442.0
18	13607.0
19	12599.0
20	13068.0
21	13629.0
22	14897.0
23	17134.0
24	19911.0
25	23135.0
26	27413.0
27	31786.0
28	37409.0
29	43769.0
30	51855.0
31	62493.0
32	76511.0
33	98403.0
34	133764.0
35	192215.0
36	309145.0
37	538598.0
38	557007.0
39	98229.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58305030163004	18.365618294769874	13.05056375421739	25.0007676493827
2	16.20188544268951	19.744487683197686	39.013007831665995	25.040619042446803
3	19.006998805630055	23.35246048073671	29.85115880850643	27.789381905126803
4	13.404069647679224	15.848891951715538	36.46151138010719	34.28552702049805
5	14.293632522853612	36.84126754070095	34.62247584121773	14.242624095227715
6	33.6789750450003	35.26615958907776	16.937554296990363	14.117311068931585
7	28.630526781509978	31.721392044114687	21.24291898957247	18.40516218480286
8	26.225037797273355	34.59123357797843	20.2858405930176	18.89788803173062
9	26.552364027792542	15.205240220435993	19.776397599217905	38.46599815255357
10-11	25.40386386507409	25.126595509544003	28.37833664813906	21.09120397724285
12-13	25.809343698083598	22.861315688401646	27.82221204015031	23.50712857336445
14-15	23.216672254761722	24.608828666010325	25.02066284729262	27.153836231935337
16-17	23.320929605726178	27.425090968988187	26.05900256060856	23.194976864677077
18-19	23.317110107333054	26.823288233189253	27.26972950393834	22.589872155539354
20-21	23.843609799374484	26.147649449070613	27.104677518674702	22.904063232880198
22-23	23.75864598724543	25.98600617866692	26.713758269718674	23.541589564368977
24-25	23.560180806079735	26.14780730416278	26.794353581949494	23.497658307807992
26-27	23.371400891894897	26.298321735754893	26.837516484857517	23.492760887492693
28-29	23.531977935641756	26.423274252247403	26.694194088348016	23.35055372376283
30-31	23.787138834745228	26.215790069610605	26.495448969222174	23.501622126421992
32-33	23.526893021535827	26.298407700766617	26.414643074497686	23.76005620319987
34-35	23.948936093765	26.29122715024561	26.444025131104038	23.315811624885356
36-37	23.960778487038688	26.043413205027637	26.506052503937195	23.489755803996477
38-39	23.69409866491957	26.165952089956757	26.53812706983502	23.601822175288653
40-41	23.91569421469045	26.0507533851808	26.562198252954783	23.471354147173965
42-43	23.761077342807006	26.0395422099633	26.36773823848561	23.83164220874408
44-45	23.70680336105586	26.079235067458693	26.47119324008568	23.742768331399763
46-47	23.82886908724454	26.071612210477834	26.25581650904069	23.84370219323693
48-49	23.582588513378816	26.18463083191727	26.345331531225053	23.887449123478863
50-51	23.654086757716865	26.183407541644666	26.410232946089963	23.75227275454851
52-53	23.824050535108952	26.113350681460336	26.35324392221698	23.709354861213733
54-55	23.456124512941532	25.972776108565515	26.602365634872204	23.96873374362075
56-57	23.486498635601656	26.109789105704444	26.444517249029374	23.959195009664526
58-59	23.530112324569053	25.95410240684788	26.553948239548227	23.96183702903484
60-61	23.544106597524273	25.914836917806756	26.70863150802966	23.83242497663931
62-63	23.543558050271205	25.922978946523557	26.692971947951023	23.84049105525422
64-65	23.380428810129715	25.87422619706213	26.57829544879164	24.167049544016518
66-67	23.307975008222954	25.90850975334141	26.5225965254694	24.260918712966237
68-69	23.419112097440326	25.795602959362675	26.58779783251342	24.19748711068358
70-71	23.32392828160555	25.616353389943647	26.57795242873836	24.48176589971245
72-73	23.295972042695794	25.702931515218452	26.553644558866303	24.447451883219447
74-75	23.266069978641493	25.826577932294096	26.5640370764162	24.343315012648212
76-77	23.315015626022507	25.54038549126576	26.657411246123498	24.48718763658824
78-79	23.205844281765707	25.55230398126973	26.728440743434412	24.51341099353015
80-81	23.34303483142295	25.660328025300295	26.570506983146704	24.42613016013005
82-83	23.21063759071542	25.599586458465414	26.740614475304174	24.449161475514995
84-85	23.222821702914022	25.599844856472544	26.695189669151553	24.48214377146188
86-87	23.17863513685644	25.631371280736587	26.700901333212272	24.489092249194705
88-89	23.1240802797919	25.396629779379698	26.767358737072332	24.71193120375607
90-91	23.04257781796421	25.37862686208779	26.872461642070196	24.706333677877808
92-93	23.146591213486133	25.175164528601453	27.02275825794393	24.655485999968484
94-95	23.016036188067197	25.037902250648962	27.078883578467693	24.867177982816145
96-97	23.06488912435036	24.84821583845659	27.106813689174476	24.980081348018572
98-99	22.937753632146464	24.844502730452703	27.103469065905855	25.114274571494978
100-101	23.09169896718995	24.679907301672348	27.2250966107854	25.003297120352304
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	54.5
2	52.5
3	69.5
4	78.5
5	94.5
6	145.0
7	292.0
8	438.0
9	496.5
10	483.5
11	465.5
12	524.0
13	607.0
14	698.0
15	776.0
16	869.0
17	991.0
18	1087.0
19	1190.5
20	1338.0
21	1493.0
22	1673.5
23	1900.5
24	2176.0
25	2679.5
26	3548.5
27	4936.0
28	7074.0
29	10186.5
30	14421.5
31	19701.5
32	26820.0
33	32425.0
34	36521.0
35	42492.5
36	49796.0
37	57115.5
38	64045.5
39	70725.5
40	76772.5
41	82357.5
42	88250.5
43	94284.5
44	99686.0
45	105943.5
46	109941.5
47	112396.5
48	115224.0
49	115823.0
50	114633.5
51	112648.0
52	110710.0
53	108919.5
54	105551.0
55	99631.0
56	92261.0
57	83113.0
58	74503.5
59	65432.5
60	53844.5
61	44046.0
62	36359.0
63	27940.5
64	20198.5
65	14886.0
66	11267.5
67	8692.5
68	6693.0
69	5054.0
70	3809.5
71	2838.0
72	2003.0
73	1487.5
74	1153.0
75	842.5
76	587.5
77	406.5
78	270.0
79	195.0
80	142.5
81	107.5
82	90.5
83	63.5
84	56.0
85	55.5
86	44.5
87	38.0
88	34.5
89	30.5
90	24.0
91	17.0
92	14.5
93	17.0
94	15.5
95	11.0
96	8.5
97	7.5
98	6.5
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05542724827477544
2	0.07638555957381274
3	0.07275528065237234
4	0.0733915151025217
5	0.06826421394543578
6	0.012051028996946449
7	0.014558541241652697
8	0.018039117939528535
9	0.006549472280949158
10-11	0.013903594013557782
12-13	0.022792163537703067
14-15	0.04171078204067334
16-17	0.02735808135642191
18-19	0.010741134540756618
20-21	0.02155712019329551
22-23	0.03766882200443043
24-25	0.03489933086848622
26-27	0.02170682241686006
28-29	0.01242528455585783
30-31	0.012107167330783156
32-33	0.008869856746199715
34-35	0.00533314171448717
36-37	7.110855619316228E-4
38-39	0.004098098370079615
40-41	0.001160192232625279
42-43	0.23722188601597846
44-45	0.20537273795261998
46-47	0.21100528411423627
48-49	0.21835940584684488
50-51	0.20969538965804643
52-53	0.21650684083023355
54-55	0.2136999241383982
56-57	0.21622614916105
58-59	0.2259006553589092
60-61	0.2308408287365394
62-63	0.21381220080607163
64-65	0.21542149970939056
66-67	0.21160409300849448
68-69	0.20653293018524527
70-71	0.19906653178496325
72-73	0.2103129113302502
74-75	0.20905915520789708
76-77	0.2030523534873694
78-79	0.2212598864284081
80-81	0.2332173515356267
82-83	0.23267468097520522
84-85	0.226518177031113
86-87	0.2485618294509933
88-89	0.24021593048726952
90-91	0.24760747777576927
92-93	0.24240532550690108
94-95	0.2455864977576478
96-97	0.25282834282258304
98-99	0.24590461498272248
100-101	0.25324002393738554
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2671971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.68617327971997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.19710765585192	51.07446894702944
2	11.30590843418342	14.400600872481059
3	3.5967961727450315	6.87198552927821
4	1.60794485365242	4.09615418295768
5	0.8820444505144415	2.808701785793906
6	0.5501100051836958	2.1020640667821904
7	0.36173475707843084	1.612625169441605
8	0.2621292617131809	1.3355207666520565
9	0.19672499847074323	1.1275796106954359
>10	0.9870736296061157	10.86196673156506
>50	0.04012151895064259	1.697482775221302
>100	0.010831680081307284	1.1997757099098547
>500	9.424524599912867E-4	0.4144088083560439
>1k	5.30129508696243E-4	0.3966650438362122
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1227666767341412E-4
2	0.0	0.0	0.0	0.0	1.1227666767341412E-4
3	0.0	0.0	0.0	0.0	1.1227666767341412E-4
4	0.0	0.0	0.0	0.0	1.4970222356455216E-4
5	0.0	0.0	0.0	0.0	1.4970222356455216E-4
6	0.0	0.0	0.0	0.0	1.4970222356455216E-4
7	0.0	0.0	0.0	0.0	1.4970222356455216E-4
8	0.0	0.0	0.0	0.0	1.871277794556902E-4
9	0.0	0.0	0.0	0.0	1.871277794556902E-4
10-11	0.0	0.0	0.0	0.0	2.2455333534682824E-4
12-13	0.0	0.0	0.0	0.0	2.4326611329239729E-4
14-15	0.0	0.0	0.0	0.0	2.619788912379663E-4
16-17	0.0	0.0	0.0	0.0	2.619788912379663E-4
18-19	0.0	0.0	0.0	0.0	2.994044471291043E-4
20-21	0.0	0.0	0.0	0.0	3.1811722507467334E-4
22-23	0.0	0.0	0.0	3.742555589113804E-5	3.742555589113804E-4
24-25	0.0	0.0	0.0	1.871277794556902E-4	4.1168111480251846E-4
26-27	0.0	0.0	0.0	6.736600060404847E-4	4.1168111480251846E-4
28-29	0.0	0.0	0.0	0.003480576697875838	4.1168111480251846E-4
30-31	0.0	0.0	0.0	0.015007647912346354	4.1168111480251846E-4
32-33	0.0	0.0	0.0	0.04811055209805795	4.491066706936565E-4
34-35	0.0	0.0	0.0	0.0973064453169589	4.491066706936565E-4
36-37	0.0	0.0	0.0	0.17187686543005146	4.491066706936565E-4
38-39	0.0	0.0	0.0	0.2880457909161439	4.491066706936565E-4
40-41	0.0	0.0	0.0	0.4150119892768297	4.491066706936565E-4
42-43	0.0	0.0	0.0	0.5503053738232937	4.678194486392255E-4
44-45	0.0	0.0	0.0	0.7286381476445665	4.865322265847945E-4
46-47	0.0	3.742555589113804E-5	0.0	0.9379779945216471	4.865322265847945E-4
48-49	0.0	3.742555589113804E-5	0.0	1.1444360735951102	4.865322265847945E-4
50-51	0.0	3.742555589113804E-5	0.0	1.3531771115779325	4.865322265847945E-4
52-53	0.0	3.742555589113804E-5	0.0	1.5624046817873398	5.426705604215016E-4
54-55	0.0	3.742555589113804E-5	0.0	1.817216579072153	5.800961163126396E-4
56-57	0.0	3.742555589113804E-5	0.0	2.108780372242064	5.988088942582087E-4
58-59	0.0	7.485111178227608E-5	0.0	2.4113098532880786	5.988088942582087E-4
60-61	0.0	7.485111178227608E-5	0.0	2.7188730716014504	5.988088942582087E-4
62-63	0.0	7.485111178227608E-5	0.0	3.0352312955492406	5.988088942582087E-4
64-65	0.0	7.485111178227608E-5	0.0	3.3866572653670266	5.988088942582087E-4
66-67	0.0	7.485111178227608E-5	0.0	3.8079380352556225	6.362344501493467E-4
68-69	0.0	7.485111178227608E-5	0.0	4.244320016946292	6.736600060404847E-4
70-71	0.0	7.485111178227608E-5	0.0	4.695466380436015	6.923727839860537E-4
72-73	0.0	7.485111178227608E-5	0.0	5.161489402392466	7.110855619316227E-4
74-75	0.0	7.485111178227608E-5	0.0	5.66364679856181	7.297983398771917E-4
76-77	0.0	7.485111178227608E-5	0.0	6.227968043066335	7.485111178227608E-4
78-79	0.0	1.4970222356455216E-4	0.0	6.854995806466462	8.046494516594678E-4
80-81	0.0	1.4970222356455216E-4	0.0	7.4915296610629385	8.60787785496175E-4
82-83	0.0	1.4970222356455216E-4	0.0	8.144568185807406	9.35638897278451E-4
84-85	0.0	1.871277794556902E-4	0.0	8.842161834840272	9.35638897278451E-4
86-87	0.0	1.871277794556902E-4	0.0	9.580044094789951	9.35638897278451E-4
88-89	0.0	1.871277794556902E-4	0.0	10.381362671975108	9.35638897278451E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1985	0.0	49.014782	1
GTATCAA	3785	0.0	45.642483	1
TCAACGC	4670	0.0	36.590485	4
CAACGCA	4760	0.0	35.794907	5
ATCAACG	4810	0.0	35.42481	3
TATCAAC	5005	0.0	33.854958	2
AACGCAG	5065	0.0	33.7142	6
ACGCAGA	5915	0.0	28.231012	7
GTACATG	6195	0.0	27.120373	1
CGCAGAG	6160	0.0	26.800642	8
TACATGG	6680	0.0	24.868507	2
ACATGGG	6790	0.0	23.696712	3
CATGGGG	5815	0.0	21.222971	4
GCAGAGT	7160	0.0	21.070213	9
AGAGTAC	5710	0.0	19.649426	10-11
GTATAAT	860	0.0	18.763515	1
CAGAGTA	6435	0.0	17.546206	10-11
GAGTACT	3415	0.0	17.226053	12-13
GAGTACA	4885	0.0	16.80797	1
GTATAGC	910	0.0	16.689463	1
>>END_MODULE