FastQCFastQC Report
Mon 15 Aug 2016
SRR2921678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671971
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.046.02491
GTATCAA39350.039.504991
TCAACGC47800.031.8720554
CAACGCA48550.031.6053835
ATCAACG48950.031.1238563
TATCAAC50750.030.3940352
AACGCAG53100.028.9801466
GTACATG62200.023.845871
ACGCAGA62200.023.823097
CGCAGAG63800.022.6301148
TACATGG66750.021.2598882
ACATGGG66300.020.8314973
CCCTATA3900.020.6883262
GCAGAGT72300.019.3134179
CATGGGG55250.018.7265224
AGAGTAC60950.018.39028210-11
GAGTACA52300.017.4521311
CAGAGTA67350.016.88954710-11
GAGTACT34150.016.53606812-13
AGTACAT53950.015.4832552
Produced by FastQC (version 0.11.5)