##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921678_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2671971
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.398511810195544	33.0	31.0	34.0	29.0	34.0
2	31.538252847804113	34.0	31.0	34.0	28.0	34.0
3	31.60805712337447	34.0	31.0	34.0	28.0	34.0
4	35.09194486017999	37.0	35.0	37.0	32.0	37.0
5	34.8420349622058	37.0	35.0	37.0	32.0	37.0
6	34.81593737357179	37.0	35.0	37.0	32.0	37.0
7	34.700280429690295	37.0	35.0	37.0	32.0	37.0
8	34.64678471435506	37.0	35.0	37.0	32.0	37.0
9	36.23586258982601	39.0	37.0	39.0	32.0	39.0
10-11	36.129876783842334	39.0	37.0	39.0	32.0	39.0
12-13	36.05930696852623	39.0	37.0	39.0	31.5	39.0
14-15	37.29978412939362	40.0	38.0	41.0	31.0	41.0
16-17	37.26380675538769	40.0	38.0	41.0	31.0	41.0
18-19	37.22681103200596	40.0	38.0	41.0	31.0	41.0
20-21	37.16650611103189	40.0	38.0	41.0	31.0	41.0
22-23	37.083000152322015	40.0	37.5	41.0	31.0	41.0
24-25	36.998661474993554	40.0	37.0	41.0	30.5	41.0
26-27	36.86645064635806	40.0	37.0	41.0	30.0	41.0
28-29	36.74200749184777	40.0	37.0	41.0	30.0	41.0
30-31	36.58215452188665	40.0	37.0	41.0	30.0	41.0
32-33	36.521387021041775	40.0	37.0	41.0	30.0	41.0
34-35	36.640663764689066	40.0	37.0	41.0	30.0	41.0
36-37	36.625618316965266	40.0	37.0	41.0	30.0	41.0
38-39	36.511222427189516	40.0	37.0	41.0	30.0	41.0
40-41	36.42864013119903	40.0	37.0	41.0	30.0	41.0
42-43	36.299014285708935	40.0	36.0	41.0	29.0	41.0
44-45	36.16179909886746	40.0	36.0	41.0	28.5	41.0
46-47	35.999224729609715	40.0	36.0	41.0	28.0	41.0
48-49	35.84760238790017	40.0	35.5	41.0	28.0	41.0
50-51	35.69269146259447	40.0	35.0	41.0	27.0	41.0
52-53	35.519809908116514	39.0	35.0	41.0	27.0	41.0
54-55	35.305178087636435	39.0	35.0	41.0	26.0	41.0
56-57	35.078833939440216	39.0	35.0	41.0	26.0	41.0
58-59	34.83737641613625	38.5	35.0	40.5	26.0	41.0
60-61	34.56356580965886	38.0	34.0	40.0	26.0	41.0
62-63	34.26148955209469	37.5	34.0	40.0	25.0	41.0
64-65	33.928648177693546	37.0	34.0	40.0	24.0	41.0
66-67	33.58048328368834	36.5	34.0	39.5	23.0	41.0
68-69	33.21984744594907	36.0	33.5	39.0	22.5	41.0
70-71	32.82054408524644	35.5	33.0	39.0	22.0	40.0
72-73	32.458489631811126	35.0	33.0	37.5	21.0	40.0
74-75	32.05294892796367	35.0	33.0	37.0	20.0	39.0
76-77	29.331927255198504	33.0	29.0	34.5	13.0	36.5
78-79	30.895236138416173	34.5	31.5	36.0	15.5	37.5
80-81	31.07141469724035	35.0	32.0	36.0	18.0	37.0
82-83	30.889518074859346	35.0	32.0	35.0	16.0	37.0
84-85	30.68070312140364	35.0	32.0	35.0	12.0	36.5
86-87	30.459035296416015	35.0	32.0	35.0	7.5	36.0
88-89	30.178841012870276	35.0	31.5	35.0	2.0	36.0
90-91	29.970979288323115	34.0	31.0	35.0	2.0	35.0
92-93	29.809594490359366	34.0	31.0	35.0	2.0	35.0
94-95	29.70082815270076	34.0	31.0	35.0	2.0	35.0
96-97	29.486401237139177	34.0	31.0	35.0	2.0	35.0
98-99	29.267802869117965	34.0	31.0	35.0	2.0	35.0
100-101	28.151380011235155	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47042.0
3	15893.0
4	9022.0
5	6314.0
6	5601.0
7	5746.0
8	6062.0
9	6628.0
10	7424.0
11	8102.0
12	8791.0
13	9307.0
14	10211.0
15	11015.0
16	11841.0
17	12551.0
18	11922.0
19	10580.0
20	10216.0
21	10084.0
22	10795.0
23	12026.0
24	14124.0
25	17014.0
26	20601.0
27	24670.0
28	29531.0
29	35880.0
30	43365.0
31	53763.0
32	67838.0
33	89521.0
34	125593.0
35	186790.0
36	324528.0
37	624320.0
38	677393.0
39	89867.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.501984259383526	19.876070948988076	13.665860166883226	25.956084624745174
2	15.301375128229408	21.05009451831215	37.580787801056076	26.06774255240237
3	17.653430629832137	24.391510111928902	29.71494511351591	28.24011414472305
4	13.10349809379302	17.069142203115657	35.430672528387255	34.39668717470408
5	14.103645404583046	37.08715032226112	34.71881093752398	14.090393335631857
6	33.6070074137893	35.83372662208687	16.708103894101342	13.851162070022491
7	28.490963596148163	31.880822015217237	21.314460865952835	18.31375352268177
8	26.128539568730346	35.17800155765163	20.01840588838726	18.675052985230753
9	26.263431101397096	15.30844283431326	19.70628412744155	38.721841936848094
10-11	25.28558609108911	25.40883534046318	28.411301636015367	20.89427693243234
12-13	25.713679418465002	22.972904190664316	27.891946857048545	23.421469533822137
14-15	23.05190424478172	24.804558367877338	25.02173133168665	27.12180605565429
16-17	23.30029467016945	27.647891322153445	25.989789183675494	23.06202482400161
18-19	23.25345349616195	27.032706695160424	27.246578316048698	22.467261492628925
20-21	23.81034361124995	26.294738484897877	27.075383094460488	22.819534809391687
22-23	23.696683085258037	26.051611338596114	26.71720613734206	23.53449943880379
24-25	23.491937551343494	26.28128093474628	26.796633925346796	23.43014758856343
26-27	23.315333037289378	26.431878732011167	26.831107670364222	23.421680560335236
28-29	23.462111534832506	26.58081785922677	26.66733162938197	23.289738976558755
30-31	23.721880395468858	26.297462579718417	26.487205281652248	23.493451743160477
32-33	23.471082423001562	26.375341783807354	26.359078395520363	23.794497397670725
34-35	23.87516611648525	26.397984500099092	26.429780800162593	23.297068583253065
36-37	23.870154003347857	26.180943407348632	26.476673999268304	23.47222859003521
38-39	23.662550978285317	26.289731437953478	26.51688584943474	23.53083173432646
40-41	23.857146840432183	26.17100752972677	26.469262333045705	23.502583296795343
42-43	23.719031278633153	26.16624895947492	26.31326991370379	23.80144984818814
44-45	23.653085533721402	26.147730962381853	26.45095549506743	23.74822800882931
46-47	23.7206484869648	26.197162223916408	26.23898669139831	23.843202597720484
48-49	23.533335217265755	26.246441192394478	26.329140835114806	23.891082755224964
50-51	23.600656612383116	26.243966831044325	26.373144763119523	23.782231793453036
52-53	23.776464692472686	26.130549356284217	26.28585030403356	23.80713564720954
54-55	23.370584494788844	26.06636268401486	26.51176351482093	24.051289306375363
56-57	23.38831033626418	26.20702096956175	26.349268373747805	24.05540032042627
58-59	23.4186993475196	26.04660659846843	26.46375760810713	24.070936445904838
60-61	23.379064479606253	26.06525624350853	26.583975072751258	23.971704204133957
62-63	23.35831822429653	26.111279249251663	26.499762578963313	24.030639947488496
64-65	23.163814943480038	26.051300502963358	26.380256923725803	24.4046276298308
66-67	23.13852146751515	26.158046718447896	26.282750079858836	24.42068173417812
68-69	23.268091843212982	26.068063310475313	26.29793532691796	24.365909519393742
70-71	23.157577975164347	25.917030477736336	26.261042135669914	24.664349411429402
72-73	23.145835466536433	26.064993262042073	26.19535342035367	24.593817851067826
74-75	23.129564810481984	26.173532990173136	26.079475900795508	24.61742629854937
76-77	23.13009396539128	25.911148432756608	26.245674003846247	24.713083598005863
78-79	23.076047134955903	25.98936528654374	26.220387776915615	24.714199801584737
80-81	23.155313771929432	26.11985966363014	26.069151015895702	24.65567554854473
82-83	23.081082730263986	26.1099467649322	26.124902223309515	24.684068281494294
84-85	23.04602524874199	26.149517231876047	26.041975392315216	24.762482127066747
86-87	22.982789259719077	26.250114694575366	25.970222479387743	24.79687356631781
88-89	22.81597351261867	26.093205100772927	26.014148375883572	25.076673010724836
90-91	22.693352538932537	26.02600827049562	26.14281480280193	25.137824387769907
92-93	22.776660961824742	25.878432383581064	26.25204616971446	25.092860484879736
94-95	22.65301512507914	25.806021173501325	26.23004141388489	25.310922287534645
96-97	22.60921090629579	25.686893268843676	26.224223897868626	25.47967192699191
98-99	22.479587028664053	25.734921235515746	26.133992626367302	25.651499109452896
100-101	22.371679296248924	25.81711791030392	26.131081309933595	25.680121483513553
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	51.0
2	46.5
3	58.0
4	61.0
5	70.0
6	110.5
7	219.0
8	339.0
9	374.5
10	340.0
11	324.5
12	332.0
13	334.0
14	373.5
15	411.5
16	447.5
17	496.0
18	578.5
19	681.0
20	811.5
21	935.0
22	1093.5
23	1387.0
24	1774.5
25	2326.5
26	3227.5
27	4614.5
28	6893.5
29	10289.0
30	14719.5
31	20118.5
32	27452.5
33	33307.0
34	37279.5
35	42643.0
36	50055.0
37	57613.0
38	64185.0
39	70676.0
40	76808.0
41	82877.0
42	88570.0
43	94362.5
44	100265.5
45	106813.0
46	111064.5
47	113579.0
48	116866.0
49	117768.0
50	116100.0
51	113852.0
52	111937.0
53	110435.5
54	107147.0
55	100490.5
56	92287.0
57	83075.5
58	74139.5
59	64487.5
60	52727.0
61	42928.5
62	35436.0
63	26983.5
64	19088.5
65	13922.0
66	10556.0
67	8130.5
68	6120.0
69	4627.5
70	3483.0
71	2497.5
72	1691.5
73	1213.5
74	931.5
75	633.5
76	402.0
77	243.0
78	123.5
79	79.0
80	52.5
81	27.5
82	16.0
83	14.5
84	12.5
85	7.5
86	4.0
87	3.0
88	4.0
89	3.0
90	1.5
91	0.5
92	0.5
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.167554213724625
2	7.485111178227608E-5
3	0.010292027870062961
4	0.006474621169166881
5	0.02582363356488525
6	0.0021706822416860064
7	3.742555589113804E-5
8	0.0
9	3.742555589113804E-5
10-11	0.05114202212524013
12-13	2.994044471291043E-4
14-15	0.005146013935031481
16-17	1.871277794556902E-5
18-19	0.0015344477915366598
20-21	4.678194486392255E-4
22-23	0.0
24-25	5.988088942582087E-4
26-27	0.002638501690325232
28-29	0.005314428936541602
30-31	0.017159617376086792
32-33	0.012200731220511001
34-35	0.010348166203899669
36-37	0.004247800593644167
38-39	0.0
40-41	0.0035367150317125447
42-43	0.009094410081546543
44-45	0.0070547172854795205
46-47	0.0033683000302024237
48-49	0.009524803974294631
50-51	0.002806916691835353
52-53	0.002320384465250558
54-55	0.004041960036242909
56-57	0.008140058406322523
58-59	0.014839232910836232
60-61	0.007335408954663056
62-63	0.020827321853418318
64-65	0.04118682425819741
66-67	0.06115335832611956
68-69	0.05069291545454648
70-71	0.06953668284573448
72-73	0.03403854308299005
74-75	0.026983825797510526
76-77	0.23411556487701402
78-79	0.0867337257777124
80-81	0.06792738394241554
82-83	0.026403729681197887
84-85	0.05174083101949834
86-87	0.06871332061612945
88-89	0.3016874060384638
90-91	0.30556095107319653
92-93	0.053612108814055245
94-95	0.010572719539246496
96-97	0.016149127367026065
98-99	6.736600060404847E-4
100-101	1.3098944561898315E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2671971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.27432210207753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.9476865062613	49.164136587047565
2	12.178490167795273	15.168144388525343
3	3.7490934254514197	7.004167547020286
4	1.6595150278796107	4.133806935176522
5	0.9088146217401666	2.8297907242662443
6	0.5536887611391108	2.068835535328635
7	0.39491896749569066	1.7215317690232566
8	0.27472973206835344	1.3686886260673679
9	0.2048885415211606	1.1483365526742275
>10	1.0719275978200946	11.530645319955516
>50	0.04343367261380416	1.8197709664009891
>100	0.011247661731444406	1.217097596095737
>500	0.0010836806418009002	0.472023422075289
>1k	4.816358407648822E-4	0.353024030343116
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	3.742555589113804E-5
4	0.0	0.0	0.0	0.0	3.742555589113804E-5
5	0.0	3.742555589113804E-5	0.0	0.0	3.742555589113804E-5
6	0.0	3.742555589113804E-5	0.0	0.0	3.742555589113804E-5
7	0.0	3.742555589113804E-5	0.0	0.0	3.742555589113804E-5
8	0.0	3.742555589113804E-5	0.0	0.0	7.485111178227608E-5
9	0.0	3.742555589113804E-5	0.0	0.0	1.4970222356455216E-4
10-11	0.0	3.742555589113804E-5	0.0	0.0	1.4970222356455216E-4
12-13	0.0	3.742555589113804E-5	0.0	0.0	1.6841500151012118E-4
14-15	0.0	3.742555589113804E-5	0.0	0.0	2.058405574012592E-4
16-17	0.0	3.742555589113804E-5	0.0	0.0	2.2455333534682824E-4
18-19	0.0	3.742555589113804E-5	0.0	0.0	2.2455333534682824E-4
20-21	0.0	3.742555589113804E-5	0.0	0.0	2.619788912379663E-4
22-23	0.0	3.742555589113804E-5	0.0	3.742555589113804E-5	3.5554278096581137E-4
24-25	0.0	3.742555589113804E-5	0.0	1.871277794556902E-4	4.1168111480251846E-4
26-27	0.0	3.742555589113804E-5	0.0	7.297983398771919E-4	4.303938927480875E-4
28-29	0.0	3.742555589113804E-5	0.0	0.003761268367059373	4.491066706936565E-4
30-31	0.0	3.742555589113804E-5	0.0	0.015681307918386837	4.491066706936565E-4
32-33	0.0	3.742555589113804E-5	0.0	0.050281234339743956	4.491066706936565E-4
34-35	0.0	3.742555589113804E-5	0.0	0.1006934581251069	4.865322265847945E-4
36-37	0.0	3.742555589113804E-5	0.0	0.17681703880768168	4.865322265847945E-4
38-39	0.0	3.742555589113804E-5	0.0	0.29616713654452087	5.052450045303636E-4
40-41	0.0	3.742555589113804E-5	0.0	0.42648292215746353	5.239577824759326E-4
42-43	0.0	3.742555589113804E-5	0.0	0.5653691600694768	5.239577824759326E-4
44-45	0.0	7.485111178227608E-5	0.0	0.7469205316973875	5.426705604215016E-4
46-47	0.0	1.1227666767341412E-4	0.0	0.959909370273854	5.800961163126396E-4
48-49	0.0	1.1227666767341412E-4	0.0	1.168800110480241	6.362344501493467E-4
50-51	0.0	1.1227666767341412E-4	0.0	1.381077863494776	6.362344501493467E-4
52-53	0.0	1.1227666767341412E-4	0.0	1.593355616509311	6.923727839860537E-4
54-55	0.0	1.1227666767341412E-4	0.0	1.8517042288258367	7.859366737138988E-4
56-57	0.0	1.1227666767341412E-4	0.0	2.1471415670304808	8.046494516594678E-4
58-59	0.0	1.1227666767341412E-4	0.0	2.4525153903242214	8.233622296050369E-4
60-61	0.0	1.1227666767341412E-4	0.0	2.7643638347871287	8.233622296050369E-4
62-63	0.0	1.1227666767341412E-4	0.0	3.085063423218291	8.60787785496175E-4
64-65	0.0	1.4970222356455216E-4	0.0	3.4414295664137073	8.79500563441744E-4
66-67	0.0	1.4970222356455216E-4	0.0	3.8678002119034973	9.16926119332882E-4
68-69	0.0	1.871277794556902E-4	0.0	4.310394835872096	9.35638897278451E-4
70-71	0.0	1.871277794556902E-4	0.0	4.770186502772672	9.35638897278451E-4
72-73	0.0	1.871277794556902E-4	0.0	5.245004530363541	0.0010292027870062962
74-75	0.0	1.871277794556902E-4	0.0	5.756892571064581	0.0010666283428974342
76-77	0.0	1.871277794556902E-4	0.0	6.330308225650652	0.0010853411208430032
78-79	0.0	1.871277794556902E-4	0.0	6.968601081374011	0.0010853411208430032
80-81	0.0	1.871277794556902E-4	0.0	7.615838644955353	0.0011040538987885722
82-83	0.0	1.871277794556902E-4	0.0	8.280553943137855	0.0011976177885164173
84-85	0.0	1.871277794556902E-4	0.0	8.989899216720541	0.0011976177885164173
86-87	0.0	1.871277794556902E-4	0.0	9.743444071810659	0.0012163305664619863
88-89	0.0	1.871277794556902E-4	0.0	10.559845148019946	0.0012350433444075553
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2355	0.0	46.0249	1
GTATCAA	3935	0.0	39.50499	1
TCAACGC	4780	0.0	31.872055	4
CAACGCA	4855	0.0	31.605383	5
ATCAACG	4895	0.0	31.123856	3
TATCAAC	5075	0.0	30.394035	2
AACGCAG	5310	0.0	28.980146	6
GTACATG	6220	0.0	23.84587	1
ACGCAGA	6220	0.0	23.82309	7
CGCAGAG	6380	0.0	22.630114	8
TACATGG	6675	0.0	21.259888	2
ACATGGG	6630	0.0	20.831497	3
CCCTATA	390	0.0	20.688326	2
GCAGAGT	7230	0.0	19.313417	9
CATGGGG	5525	0.0	18.726522	4
AGAGTAC	6095	0.0	18.390282	10-11
GAGTACA	5230	0.0	17.452131	1
CAGAGTA	6735	0.0	16.889547	10-11
GAGTACT	3415	0.0	16.536068	12-13
AGTACAT	5395	0.0	15.483255	2
>>END_MODULE