FastQCFastQC Report
Mon 15 Aug 2016
SRR2921677_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921677_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3112027
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.044.2800331
GTATCAA39350.042.573531
TCAACGC45450.036.0259784
CAACGCA46350.035.1188355
ATCAACG48750.033.6851963
AACGCAG50750.032.4319236
TATCAAC50850.032.2951052
GTACATG76450.028.5555151
ACGCAGA57700.027.953637
TACATGG78450.027.4067042
CGCAGAG60550.026.6408928
ACATGGG80300.025.8287563
CATGGGG69550.023.0648274
GCAGAGT73350.020.3101679
GAGTACA55000.019.0694981
AGAGTAC58100.018.74165710-11
ATGGGGG42050.017.8314555
TAAGGTG15000.017.4007175
CAGAGTA67000.016.81861710-11
TCTATAC9050.016.7818183
Produced by FastQC (version 0.11.5)