##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921677_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3112027
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.75876848112179	31.0	30.0	34.0	25.0	34.0
2	29.81610570859443	31.0	30.0	34.0	25.0	34.0
3	29.87126043572244	31.0	30.0	34.0	25.0	34.0
4	33.31218238145106	37.0	35.0	37.0	27.0	37.0
5	33.2976564792015	37.0	35.0	37.0	27.0	37.0
6	33.3343033334865	37.0	35.0	37.0	27.0	37.0
7	33.22945045142603	37.0	35.0	37.0	26.0	37.0
8	33.193367538263644	37.0	35.0	37.0	26.0	37.0
9	34.685508191284974	39.0	35.0	39.0	27.0	39.0
10-11	34.56670330945072	39.0	35.0	39.0	27.0	39.0
12-13	34.446897150956595	39.0	35.0	39.0	26.0	39.0
14-15	35.506938243145065	40.0	36.0	41.0	25.5	41.0
16-17	35.439236549040224	40.0	36.0	41.0	25.0	41.0
18-19	35.40089015937201	40.0	36.0	41.0	25.0	41.0
20-21	35.316776011262114	40.0	36.0	41.0	25.0	41.0
22-23	35.21058927187971	40.0	35.5	41.0	24.0	41.0
24-25	35.09404079720388	39.5	35.0	41.0	24.0	41.0
26-27	34.94002285327216	39.0	35.0	41.0	22.5	41.0
28-29	34.79558676708139	39.0	35.0	41.0	22.0	41.0
30-31	34.644157971637135	39.0	34.5	41.0	20.5	41.0
32-33	34.46033437370563	39.0	34.0	41.0	18.5	41.0
34-35	34.283840885699256	39.0	34.0	41.0	18.0	41.0
36-37	34.244965419644494	39.0	34.0	40.5	17.0	41.0
38-39	34.418109161649305	39.0	34.5	41.0	18.0	41.0
40-41	34.47977443640431	39.0	35.0	41.0	17.0	41.0
42-43	34.324377166393475	39.0	34.0	41.0	15.0	41.0
44-45	34.20102990751687	39.0	34.0	41.0	14.0	41.0
46-47	34.037198745383634	39.0	34.0	41.0	10.5	41.0
48-49	33.86906942002753	39.0	34.0	41.0	8.5	41.0
50-51	32.21173113215278	37.0	32.0	39.5	6.5	40.0
52-53	32.32281789328948	37.5	32.0	39.5	4.0	40.0
54-55	33.22217914561796	38.0	33.0	40.0	2.0	41.0
56-57	33.252268055514946	38.0	33.0	40.0	2.0	41.0
58-59	33.068528647084364	38.0	33.0	40.0	2.0	41.0
60-61	32.82283283531923	37.5	33.0	40.0	2.0	41.0
62-63	32.54202919833279	37.0	32.0	40.0	2.0	41.0
64-65	32.25867047426003	36.5	32.0	40.0	2.0	41.0
66-67	31.922885951824966	36.0	32.0	39.0	2.0	41.0
68-69	31.567579747862084	35.5	31.5	39.0	2.0	41.0
70-71	31.162300327085852	35.0	31.0	38.5	2.0	40.5
72-73	30.809551620214094	35.0	31.0	37.5	2.0	40.0
74-75	30.413379768234655	35.0	31.0	37.0	2.0	39.0
76-77	30.043854375299443	35.0	30.0	36.5	2.0	39.0
78-79	29.699639334748703	35.0	30.0	36.0	2.0	38.5
80-81	29.383787319325954	35.0	30.0	35.5	2.0	37.0
82-83	29.068157024344583	35.0	30.0	35.0	2.0	37.0
84-85	28.802494965499978	34.0	29.0	35.0	2.0	36.5
86-87	28.567338104714388	34.0	29.0	35.0	2.0	36.0
88-89	28.340366262889106	34.0	29.0	35.0	2.0	36.0
90-91	28.11326797614545	34.0	29.0	35.0	2.0	35.5
92-93	27.918055498875813	34.0	29.0	35.0	2.0	35.0
94-95	27.68239912442919	34.0	29.0	35.0	2.0	35.0
96-97	27.439897211688717	34.0	28.5	35.0	2.0	35.0
98-99	27.15294902647053	34.0	27.0	35.0	2.0	35.0
100-101	26.08206130602337	33.0	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	143458.0
3	23174.0
4	15497.0
5	11559.0
6	10551.0
7	10598.0
8	11132.0
9	11723.0
10	12977.0
11	13637.0
12	13473.0
13	13548.0
14	14033.0
15	14859.0
16	15072.0
17	15719.0
18	15009.0
19	14508.0
20	14434.0
21	15037.0
22	16907.0
23	19205.0
24	22578.0
25	26655.0
26	31440.0
27	36861.0
28	43277.0
29	50628.0
30	60361.0
31	72572.0
32	89948.0
33	114991.0
34	156204.0
35	222908.0
36	362376.0
37	624425.0
38	638328.0
39	112350.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.294161446591914	17.90364541471463	12.53042989582141	25.27176324287204
2	15.950939578712187	19.21546029677812	39.56176574812898	25.271834376380713
3	19.07039723670371	23.408723534997325	29.395040176416735	28.125839051882224
4	13.015594030434341	15.380326363352228	36.74931862936138	34.85476097685205
5	14.022989392751162	37.19260473247476	34.79064437372731	13.99376150104677
6	34.016184685768934	35.365219154631205	16.814693671351446	13.803902488248413
7	29.08492447001747	31.50412069371117	21.1269914115256	18.283963424745757
8	26.447307569068357	35.21415103041312	19.790012530857116	18.54852886966141
9	26.699219911145388	15.117752444305191	19.2409598868831	38.94206775766632
10-11	25.71272750879119	24.989410721941304	28.18555693175106	21.112304837516447
12-13	26.10355013372883	22.52633798337594	27.784083577025736	23.586028305869494
14-15	23.19158521819789	24.157474665372686	24.858208219945183	27.792731896484245
16-17	23.612188760970866	27.209296495171948	25.71186496245493	23.466649781402253
18-19	23.626987929814955	26.660265056237442	26.821960351792917	22.89078666215469
20-21	24.192195666759066	25.884079979751963	26.738627552598697	23.18509680089027
22-23	23.96471378246123	25.8121998660839	26.39666039088972	23.82642596056515
24-25	23.758964017602814	25.96834855388793	26.498519196285287	23.774168232223968
26-27	23.635620028813698	26.108825138785434	26.426113276557654	23.82944155584321
28-29	23.79397397944599	26.284207875839726	26.282665288622088	23.6391528560922
30-31	23.965196644614085	26.039511462392667	26.176705379197045	23.8185865137962
32-33	23.70289898968637	26.111104773140188	26.12482692652847	24.06116931064497
34-35	24.11643046326545	26.11028238362449	26.16942696118663	23.60386019192343
36-37	24.187811765998788	25.87149878559175	26.22014810687435	23.72054134153511
38-39	23.950679093565018	26.03504508732672	26.194673970658815	23.819601848449448
40-41	24.07870133383976	25.932561544964916	26.263233882595493	23.725503238599828
42-43	23.93354915921947	25.84305108065387	26.186773987011797	24.03662577311486
44-45	23.891849287800518	25.942989590961606	26.2650050798374	23.900156041400475
46-47	24.056729833617645	25.954797217279758	26.060520881027312	23.927952068075285
48-49	23.81154172496349	26.06248883035503	26.05976786458929	24.06620158009219
50-51	23.894159917649812	26.082303177868482	26.159739915595566	23.863796988886136
52-53	24.115647118280965	25.939536636780762	26.121308947399857	23.82350729753841
54-55	23.609466493456562	25.841577798281495	26.453555344507844	24.095400363754095
56-57	23.720889645658996	25.99050230615959	26.29411552565111	23.994492522530297
58-59	23.788099670596388	25.827478038417436	26.386391821232607	23.998030469753573
60-61	23.735869886211947	25.8982551296085	26.488852905761025	23.877022078418527
62-63	23.767447356863087	25.894939767415803	26.44890180021561	23.8887110755055
64-65	23.57546295863986	25.798028286111997	26.403510664365893	24.222998090882246
66-67	23.589726829832163	25.88302513940892	26.31669527802334	24.210552752735573
68-69	23.75759236366265	25.71788677085015	26.416716666647883	24.107804198839315
70-71	23.67048406779792	25.549763874667246	26.40199466627346	24.377757391261376
72-73	23.580240970760656	25.69926002892396	26.3209175171244	24.399581483190985
74-75	23.556543467302987	25.853674400079463	26.302613639052918	24.28716849356463
76-77	23.61009178668759	25.539146377527146	26.427167326984986	24.42359450880028
78-79	23.529069853563907	25.543926640909238	26.48160516161941	24.44539834390745
80-81	23.672536339038434	25.6420995813726	26.319484668176152	24.36587941141281
82-83	23.61991967385175	25.67792699151168	26.398662712300542	24.303490622336028
84-85	23.564825966712053	25.69177603994245	26.420746937140372	24.32265105620513
86-87	23.56982084269833	25.67870861927576	26.379674204402047	24.371796333623866
88-89	23.449389011974343	25.557338764767433	26.424648352968493	24.568623870289727
90-91	23.38773005739759	25.54530930923193	26.498151166311285	24.568809467059193
92-93	23.532682765161123	25.338066612138626	26.679768856500587	24.449481766199668
94-95	23.392941318191138	25.181889466668533	26.794331113635238	24.630838101505088
96-97	23.45305616793301	25.07310412449637	26.76965452462831	24.704185182942307
98-99	23.332023493375377	25.030783923110246	26.76806653128075	24.869126052233625
100-101	23.47728107666635	24.87363791228233	26.85925768685588	24.78982332419544
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	15.5
2	32.5
3	65.0
4	66.5
5	66.0
6	93.5
7	192.5
8	332.0
9	399.0
10	396.5
11	413.5
12	471.0
13	537.0
14	641.0
15	721.0
16	800.0
17	917.5
18	1017.5
19	1124.0
20	1239.0
21	1356.0
22	1504.5
23	1736.5
24	2063.0
25	2594.0
26	3449.5
27	4858.5
28	7128.0
29	10289.0
30	14654.0
31	20781.5
32	29043.0
33	35442.5
34	40363.5
35	47238.5
36	56172.0
37	65006.5
38	73024.0
39	81263.5
40	88586.0
41	94850.0
42	100818.5
43	107965.5
44	114387.5
45	120776.0
46	125880.5
47	129136.5
48	133002.0
49	134884.5
50	133487.0
51	131751.0
52	130716.0
53	129303.5
54	126503.5
55	120143.0
56	110572.0
57	100332.0
58	91199.0
59	79853.5
60	66097.0
61	54780.5
62	45321.0
63	34781.0
64	25046.0
65	18376.0
66	13793.0
67	10699.0
68	8207.5
69	6110.0
70	4632.5
71	3446.0
72	2417.0
73	1723.0
74	1309.5
75	934.0
76	677.5
77	491.0
78	338.5
79	221.0
80	162.0
81	141.5
82	107.5
83	82.0
84	64.5
85	61.0
86	56.0
87	44.0
88	35.5
89	37.0
90	32.5
91	19.0
92	17.5
93	18.5
94	19.5
95	18.5
96	12.5
97	8.5
98	7.5
99	6.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05138130228304575
2	0.07162534258218196
3	0.06805853548185796
4	0.06809066887915818
5	0.063752660243629
6	0.010282687136069193
7	0.013335359879589734
8	0.016323765828509845
9	0.006490946254643678
10-11	0.012499891549784111
12-13	0.022284511027699953
14-15	0.03905814441841282
16-17	0.026767119951080113
18-19	0.00996135316306703
20-21	0.01974597264098287
22-23	0.036117938565443035
24-25	0.0336597336719765
26-27	0.021191975519492603
28-29	0.011937557097030328
30-31	0.011985757192980652
32-33	0.00869208396970849
34-35	0.005173476965334813
36-37	5.62334452753784E-4
38-39	0.003904207771976272
40-41	0.0010925355082073518
42-43	0.22670111795302544
44-45	0.19667245817597342
46-47	0.20208693562105987
48-49	0.20925268321900806
50-51	0.20134786748315486
52-53	0.20865821536895407
54-55	0.20428807333612467
56-57	0.20664987803769053
58-59	0.21587216306285262
60-61	0.22069217265788502
62-63	0.2056216093240836
64-65	0.2057822763105847
66-67	0.20325980462251775
68-69	0.198986062781589
70-71	0.19098484685383513
72-73	0.2023600694981117
74-75	0.20120326719530388
76-77	0.19522645529746369
78-79	0.2135264250599368
80-81	0.22488558100556325
82-83	0.22287724367429973
84-85	0.2171896323521615
86-87	0.23717660547289596
88-89	0.22936818992894342
90-91	0.23672673791069293
92-93	0.23144079405480736
94-95	0.23539320192273397
96-97	0.24263928301393273
98-99	0.2352807350321832
100-101	0.2427838833017837
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3112027.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.44394773568516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.71594819478402	49.77778502775679
2	11.52744911564029	14.396388602056245
3	3.6502168504095502	6.838018506926737
4	1.676267263142146	4.186909814827526
5	0.9420962663178345	2.9414105007967493
6	0.551511255298125	2.0663124000886937
7	0.3603818220129004	1.575256456106518
8	0.27252572954886967	1.361406593006327
9	0.20823130706694107	1.1702506369879329
>10	1.0324014844334084	11.30804309341741
>50	0.04787686928056539	1.9989476806222355
>100	0.013494197513359323	1.4134492255573836
>500	8.77224432281867E-4	0.39053033045066937
>1k	7.224201196744959E-4	0.5752911313987642
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.2133397300216223E-5
2	0.0	0.0	0.0	0.0	3.2133397300216223E-5
3	0.0	0.0	0.0	0.0	3.2133397300216223E-5
4	0.0	0.0	0.0	0.0	3.2133397300216223E-5
5	0.0	3.2133397300216223E-5	0.0	0.0	3.2133397300216223E-5
6	0.0	3.2133397300216223E-5	0.0	0.0	6.426679460043245E-5
7	0.0	6.426679460043245E-5	0.0	0.0	6.426679460043245E-5
8	0.0	6.426679460043245E-5	0.0	3.2133397300216223E-5	6.426679460043245E-5
9	0.0	6.426679460043245E-5	0.0	6.426679460043245E-5	1.6066698650108114E-4
10-11	0.0	6.426679460043245E-5	0.0	1.4460028785097303E-4	1.6066698650108114E-4
12-13	0.0	8.033349325054057E-5	0.0	1.6066698650108114E-4	1.6066698650108114E-4
14-15	0.0	9.640019190064868E-5	0.0	1.6066698650108114E-4	2.0886708245140546E-4
16-17	0.0	9.640019190064868E-5	0.0	1.6066698650108114E-4	2.2493378110151357E-4
18-19	0.0	9.640019190064868E-5	0.0	1.9280038380129735E-4	3.213339730021623E-4
20-21	3.2133397300216223E-5	1.285335892008649E-4	0.0	1.9280038380129735E-4	3.213339730021623E-4
22-23	3.2133397300216223E-5	1.285335892008649E-4	0.0	2.0886708245140546E-4	3.374006716522704E-4
24-25	3.2133397300216223E-5	1.285335892008649E-4	0.0	2.89200575701946E-4	4.177341649028109E-4
26-27	3.2133397300216223E-5	1.285335892008649E-4	0.0	4.820009595032434E-4	4.177341649028109E-4
28-29	3.2133397300216223E-5	1.285335892008649E-4	0.0	0.001976203933963298	4.177341649028109E-4
30-31	3.2133397300216223E-5	1.285335892008649E-4	0.0	0.010073820053617786	4.177341649028109E-4
32-33	3.2133397300216223E-5	1.285335892008649E-4	0.0	0.02953059211889871	4.4986756220302714E-4
34-35	4.820009595032434E-5	1.285335892008649E-4	0.0	0.057084980303834125	4.8200095950324336E-4
36-37	6.426679460043245E-5	1.4460028785097303E-4	0.0	0.10091493422132905	4.8200095950324336E-4
38-39	6.426679460043245E-5	1.6066698650108114E-4	0.0	0.17795475424859747	4.8200095950324336E-4
40-41	6.426679460043245E-5	1.6066698650108114E-4	0.0	0.2641686592050776	5.302010554535676E-4
42-43	6.426679460043245E-5	1.6066698650108114E-4	0.0	0.35600590868909554	5.462677541036758E-4
44-45	6.426679460043245E-5	1.6066698650108114E-4	0.0	0.4677979978965478	5.62334452753784E-4
46-47	6.426679460043245E-5	1.6066698650108114E-4	0.0	0.5980507238529743	6.266012473542164E-4
48-49	6.426679460043245E-5	1.6066698650108114E-4	0.0	0.737573934930513	7.230014392548651E-4
50-51	6.426679460043245E-5	1.6066698650108114E-4	0.0	0.8835077587694451	7.390681379049732E-4
52-53	6.426679460043245E-5	1.6066698650108114E-4	0.0	1.0354023278075672	7.390681379049732E-4
54-55	6.426679460043245E-5	1.6066698650108114E-4	0.0	1.2086977394476333	7.390681379049732E-4
56-57	6.426679460043245E-5	1.6066698650108114E-4	0.0	1.4076838022292222	7.390681379049732E-4
58-59	6.426679460043245E-5	2.2493378110151357E-4	0.0	1.6192018899578957	7.390681379049732E-4
60-61	6.426679460043245E-5	2.570671784017298E-4	0.0	1.8438143370864069	7.390681379049732E-4
62-63	6.426679460043245E-5	2.570671784017298E-4	0.0	2.069535386421776	7.390681379049732E-4
64-65	6.426679460043245E-5	2.570671784017298E-4	0.0	2.3196617510066586	7.551348365550813E-4
66-67	6.426679460043245E-5	3.534673703023785E-4	0.0	2.604829585347428	7.712015352051894E-4
68-69	6.426679460043245E-5	3.534673703023785E-4	0.0	2.9175839412704327	7.872682338552976E-4
70-71	6.426679460043245E-5	3.534673703023785E-4	0.0	3.254840012634852	8.033349325054056E-4
72-73	6.426679460043245E-5	3.534673703023785E-4	0.0	3.606491845989768	9.318685217062705E-4
74-75	6.426679460043245E-5	3.534673703023785E-4	0.0	3.966578696135991	9.640019190064867E-4
76-77	6.426679460043245E-5	3.534673703023785E-4	0.0	4.369402964691502	9.640019190064867E-4
78-79	6.426679460043245E-5	3.534673703023785E-4	0.0	4.814273783614345	9.96135316306703E-4
80-81	6.426679460043245E-5	3.856007676025947E-4	0.0	5.285847455693668	0.0010122020149568112
82-83	6.426679460043245E-5	3.856007676025947E-4	0.0	5.769631818747074	0.0010282687136069191
84-85	6.426679460043245E-5	3.856007676025947E-4	0.0	6.274993758087574	0.0010282687136069191
86-87	6.426679460043245E-5	4.177341649028109E-4	0.0	6.815124033306909	0.0010443354122570273
88-89	6.426679460043245E-5	4.177341649028109E-4	0.0	7.397316925592227	0.0010604021109071355
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2240	0.0	44.280033	1
GTATCAA	3935	0.0	42.57353	1
TCAACGC	4545	0.0	36.025978	4
CAACGCA	4635	0.0	35.118835	5
ATCAACG	4875	0.0	33.685196	3
AACGCAG	5075	0.0	32.431923	6
TATCAAC	5085	0.0	32.295105	2
GTACATG	7645	0.0	28.555515	1
ACGCAGA	5770	0.0	27.95363	7
TACATGG	7845	0.0	27.406704	2
CGCAGAG	6055	0.0	26.640892	8
ACATGGG	8030	0.0	25.828756	3
CATGGGG	6955	0.0	23.064827	4
GCAGAGT	7335	0.0	20.310167	9
GAGTACA	5500	0.0	19.069498	1
AGAGTAC	5810	0.0	18.741657	10-11
ATGGGGG	4205	0.0	17.831455	5
TAAGGTG	1500	0.0	17.400717	5
CAGAGTA	6700	0.0	16.818617	10-11
TCTATAC	905	0.0	16.781818	3
>>END_MODULE