FastQCFastQC Report
Mon 15 Aug 2016
SRR2921677_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921677_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3112027
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.040.3991551
GTATCAA36250.038.159031
TCAACGC43100.031.1638374
CAACGCA44100.030.483635
ATCAACG45950.029.5403253
AACGCAG47850.028.1907316
TATCAAC50300.026.8912892
GTACATG76700.025.967551
TACATGG79850.024.8448352
ACATGGG81550.023.5703413
ACGCAGA58850.022.7585537
CGCAGAG61150.021.5918738
CATGGGG69200.020.9873244
AGAGTAC59400.018.35009210-11
GAGTACA58650.017.58751
TAAGGTG16450.017.3262355
GCAGAGT74650.016.9236419
TATACTG14050.016.56685
GTATAGC9000.016.3731231
CAGAGTA67650.016.1473799
Produced by FastQC (version 0.11.5)