FastQCFastQC Report
Mon 15 Aug 2016
SRR2921676_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921676_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3345219
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22700.045.788831
GTATCAA42650.042.9545821
TCAACGC48450.037.321534
CAACGCA50300.035.664745
ATCAACG52350.034.452023
AACGCAG53300.033.816716
TATCAAC55400.032.7276152
GTACATG90200.028.834691
TACATGG93800.027.4256692
ACGCAGA65600.026.9739727
ACATGGG96500.025.9197653
CGCAGAG68200.025.6681698
CATGGGG82450.023.3127024
GAGTACA64700.021.2733061
GCAGAGT79600.020.7414259
AGAGTAC61500.019.7875110-11
AGTACAT69500.018.5073932
GTATATG10650.017.8259071
GTGTAAG16550.017.780131
ATGGGGG49250.017.05625
Produced by FastQC (version 0.11.5)