##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921676_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3345219
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.095597627539483	31.0	31.0	34.0	26.0	34.0
2	30.151010741000814	31.0	31.0	34.0	26.0	34.0
3	30.207644402354525	31.0	31.0	34.0	26.0	34.0
4	33.657087622663866	37.0	35.0	37.0	28.0	37.0
5	33.64968362310509	37.0	35.0	37.0	28.0	37.0
6	33.68733825797354	37.0	35.0	37.0	28.0	37.0
7	33.59180878740674	37.0	35.0	37.0	28.0	37.0
8	33.55017055684546	37.0	35.0	37.0	28.0	37.0
9	35.06983937374504	39.0	35.0	39.0	28.0	39.0
10-11	34.95830721396716	39.0	35.0	39.0	27.0	39.0
12-13	34.84330338312678	39.0	35.0	39.0	27.0	39.0
14-15	35.95186428751002	40.0	36.0	41.0	27.0	41.0
16-17	35.88635990648146	40.0	36.0	41.0	27.0	41.0
18-19	35.847393698290006	40.0	36.0	41.0	27.0	41.0
20-21	35.763745662092674	40.0	36.0	41.0	27.0	41.0
22-23	35.659361165890786	40.0	36.0	41.0	26.5	41.0
24-25	35.55195130722383	40.0	36.0	41.0	25.5	41.0
26-27	35.40161227112485	40.0	36.0	41.0	25.0	41.0
28-29	35.26108230881147	40.0	35.0	41.0	24.5	41.0
30-31	35.11009383840042	39.0	35.0	41.0	24.0	41.0
32-33	34.931541552287015	39.0	35.0	41.0	24.0	41.0
34-35	34.758018533315756	39.0	35.0	41.0	23.0	41.0
36-37	34.729311294716425	39.0	35.0	41.0	22.5	41.0
38-39	34.91213609632135	39.0	35.0	41.0	23.0	41.0
40-41	34.9737446188127	40.0	35.0	41.0	23.0	41.0
42-43	34.815543915062065	40.0	35.0	41.0	21.5	41.0
44-45	34.69428040436217	39.5	35.0	41.0	20.5	41.0
46-47	34.53270533259557	39.0	35.0	41.0	18.5	41.0
48-49	34.368217745983145	39.0	34.0	41.0	16.5	41.0
50-51	32.696587727141335	37.0	32.0	39.5	13.5	40.0
52-53	32.816305300191104	37.5	32.5	39.5	13.0	40.0
54-55	33.71758485767299	38.5	33.5	40.0	13.0	41.0
56-57	33.733230021711584	38.0	34.0	41.0	11.0	41.0
58-59	33.54278972467871	38.0	34.0	40.5	8.5	41.0
60-61	33.28922127370435	37.5	33.0	40.0	7.5	41.0
62-63	32.99236462545501	37.0	33.0	40.0	6.0	41.0
64-65	32.69960935293025	36.5	33.0	40.0	2.0	41.0
66-67	32.35829268577035	36.0	32.5	39.0	2.0	41.0
68-69	31.992731865985455	35.5	32.0	39.0	2.0	41.0
70-71	31.581440856338553	35.0	32.0	38.5	2.0	40.5
72-73	31.220467329642695	35.0	32.0	37.5	2.0	40.0
74-75	30.827075746012444	35.0	31.0	37.0	2.0	39.0
76-77	30.451021891242398	35.0	31.0	36.5	2.0	39.0
78-79	30.11258754658514	35.0	31.0	36.0	2.0	38.5
80-81	29.80670682547241	35.0	31.0	35.5	2.0	37.0
82-83	29.50167761811708	35.0	30.0	35.0	2.0	37.0
84-85	29.241474623933442	35.0	30.0	35.0	2.0	36.0
86-87	29.006603454063843	34.5	30.0	35.0	2.0	36.0
88-89	28.788831463650062	34.0	30.0	35.0	2.0	36.0
90-91	28.56739050567392	34.0	30.0	35.0	2.0	35.0
92-93	28.373608723375064	34.0	29.0	35.0	2.0	35.0
94-95	28.143749631937403	34.0	29.0	35.0	2.0	35.0
96-97	27.898185141241875	34.0	29.0	35.0	2.0	35.0
98-99	27.622284520086726	34.0	29.0	35.0	2.0	35.0
100-101	26.556499589414024	33.0	26.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	132969.0
3	22840.0
4	15023.0
5	11334.0
6	10294.0
7	10443.0
8	11078.0
9	11766.0
10	12865.0
11	13716.0
12	13770.0
13	13734.0
14	14301.0
15	14895.0
16	15100.0
17	15624.0
18	15048.0
19	14520.0
20	14710.0
21	15661.0
22	17100.0
23	19770.0
24	23279.0
25	27194.0
26	32305.0
27	37983.0
28	44588.0
29	52150.0
30	62419.0
31	75328.0
32	93282.0
33	120329.0
34	165186.0
35	238024.0
36	395798.0
37	704408.0
38	714670.0
39	121703.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.708966301928	17.497854669044752	12.51966989570506	25.273509133322186
2	15.994464179616191	19.195451197483152	39.41911581244985	25.390968810450804
3	19.246547618940777	23.22600370419898	29.147905595877337	28.37954308098291
4	13.32984315121563	15.156385088745358	36.148244986851985	35.36552677318702
5	14.279705749233514	37.1337383554157	34.32527746425744	14.261278431093347
6	34.36032920870743	34.87894443698692	16.634074458521393	14.12665189578426
7	29.48325523074099	31.520809053961045	20.671554730912302	18.32438098438566
8	26.82993481213679	35.026808557330966	19.599353114429917	18.543903516102326
9	26.968843320337804	15.272689332755753	18.891048332778773	38.86741901412766
10-11	25.979459700091827	24.930634669047283	27.774265682708677	21.31563994815221
12-13	26.32153823682476	22.321809135288216	27.627965512173763	23.728687115713264
14-15	23.43940135694738	23.929617812394113	24.5582085686798	28.072772261978706
16-17	23.843324217275935	27.0982231761556	25.42388769846562	23.634564908102845
18-19	23.952839549651873	26.499015360495846	26.57105085116681	22.977094238685467
20-21	24.37450254713805	25.679017550406062	26.48551160130338	23.46096830115251
22-23	24.26196617727824	25.504902830811183	26.226402991325642	24.00672800058494
24-25	24.05850026898497	25.711957914542623	26.16793154893179	24.06161026754062
26-27	23.846316914964422	25.903003313493834	26.22928468359752	24.021395087944228
28-29	24.085160540779956	26.098368664168987	26.034748196137	23.781722598914058
30-31	24.280269634112837	25.838211759106372	25.873490094710373	24.008028512070425
32-33	23.927397746594668	25.95640426764327	25.902920458155048	24.21327752760702
34-35	24.318552175799024	25.908933417079044	25.98192192815228	23.790592478969653
36-37	24.361348030852053	25.819157384310397	25.95423190398275	23.865262680854798
38-39	24.222786136089386	25.91279384444019	25.938966662466463	23.925453357003963
40-41	24.331081133597234	25.74289110222667	25.98117467133249	23.9448530928436
42-43	24.11641879014363	25.722875600389354	25.916504397923664	24.244201211543356
44-45	24.081163704290713	25.8026632701677	26.022023823520644	24.094149202020944
46-47	24.284862655344973	25.803000777380188	25.798447336611613	24.11368923066323
48-49	23.97724188303615	25.869861942558853	25.89628585278227	24.256610321622727
50-51	24.06970543917512	25.884551208888652	25.964714411103024	24.081028940833207
52-53	24.289973968982675	25.759996956207438	25.94819680755292	24.00183226725697
54-55	23.79429688925317	25.5905128497789	26.21936368677378	24.395826574194153
56-57	23.860265370869122	25.75439563567078	26.080791618858168	24.304547374601928
58-59	23.989481841525635	25.676833430881818	26.100828530437447	24.2328561971551
60-61	24.014046473512675	25.585495697743145	26.213961529501567	24.18649629924262
62-63	24.018708737609582	25.619361820843988	26.265015484513615	24.096913957032815
64-65	23.793703947360537	25.478411205657952	26.252970316606095	24.47491453037542
66-67	23.811216380008133	25.670512546291054	26.094299242866786	24.42397183083402
68-69	23.919608948554973	25.549624028167784	26.148330189001406	24.382436834275833
70-71	23.85950928863836	25.35107401706314	26.176569345711197	24.6128473485873
72-73	23.781926462041735	25.528467949217227	26.11085814255843	24.578747446182607
74-75	23.670060116302338	25.64196350364089	26.159548450720088	24.528427929336683
76-77	23.71077740143287	25.24518125837322	26.330372581455624	24.713668758738287
78-79	23.655046426781855	25.25416200726081	26.33733624045306	24.753455325504284
80-81	23.7740421025286	25.445852243385232	26.144629630156764	24.635476023929403
82-83	23.6782052787892	25.478766308946522	26.24697671742825	24.596051694836028
84-85	23.665857609783707	25.425833472903385	26.290623499122585	24.617685418190327
86-87	23.65038321596341	25.50097671336141	26.210425577918084	24.6382144927571
88-89	23.576470320311312	25.196507917625116	26.340668460282572	24.886353301780993
90-91	23.537215572087785	25.207541481700463	26.385416649497966	24.869826296713786
92-93	23.591477071905445	25.127852761324483	26.51297615817379	24.767694008596283
94-95	23.52091353198926	24.9275552542893	26.608078570559535	24.943452643161905
96-97	23.44984085774536	24.827810043441392	26.639818544414407	25.08253055439884
98-99	23.405252833517828	24.7575679843501	26.652178681685264	25.185000500446808
100-101	23.51351501195985	24.611756300479847	26.751492714778678	25.12323597278162
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	27.5
2	42.0
3	71.0
4	71.0
5	77.5
6	128.0
7	283.0
8	471.0
9	540.5
10	516.0
11	503.0
12	559.0
13	645.0
14	755.0
15	873.5
16	954.0
17	1068.5
18	1174.0
19	1306.5
20	1397.0
21	1445.5
22	1610.5
23	1821.0
24	2077.0
25	2494.0
26	3150.0
27	4197.5
28	6038.5
29	8808.0
30	12682.0
31	18275.5
32	26021.5
33	32256.5
34	37032.0
35	43912.5
36	53321.0
37	62816.0
38	71578.5
39	80144.5
40	88060.0
41	95777.5
42	103479.0
43	112685.5
44	122170.5
45	131721.0
46	137882.0
47	142108.0
48	147636.0
49	149669.0
50	149443.0
51	148543.5
52	146592.0
53	145743.5
54	144059.5
55	137327.0
56	126575.5
57	113938.5
58	103288.5
59	91912.5
60	75620.5
61	60866.0
62	49469.5
63	37748.5
64	27004.0
65	19531.0
66	14444.5
67	10964.0
68	8243.5
69	6042.5
70	4431.0
71	3128.0
72	2189.5
73	1562.0
74	1107.5
75	779.0
76	532.0
77	359.0
78	252.0
79	178.0
80	133.5
81	114.0
82	105.5
83	89.0
84	71.0
85	61.0
86	50.5
87	43.5
88	46.5
89	44.5
90	41.0
91	31.0
92	29.5
93	28.0
94	20.5
95	18.0
96	11.5
97	13.5
98	15.5
99	11.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05715619814427696
2	0.07847019881209572
3	0.07551075131403953
4	0.07500256335982787
5	0.07042887177192285
6	0.010462693174946094
7	0.014139582490712866
8	0.017278390443196692
9	0.006636336813822951
10-11	0.012809325787041146
12-13	0.023690526688985088
14-15	0.041551838609071634
16-17	0.02801012429978426
18-19	0.010163759084233349
20-21	0.02035741157753797
22-23	0.03836819054298089
24-25	0.034855714977106135
26-27	0.021314000667818758
28-29	0.011464122378833793
30-31	0.011658429537797077
32-33	0.008414994653563787
34-35	0.005141666360259224
36-37	8.51962158531325E-4
38-39	0.004349491019870448
40-41	0.0013452034082073552
42-43	0.236322345412961
44-45	0.20565170770583333
46-47	0.21176490986090898
48-49	0.21920836871965632
50-51	0.21012077236198887
52-53	0.2175642312207362
54-55	0.21361830122332798
56-57	0.21669732235766928
58-59	0.22606890610151384
60-61	0.23097142518920286
62-63	0.21542685247214008
64-65	0.21496350463153535
66-67	0.2122730978151206
68-69	0.2073705787274316
70-71	0.19962818577797148
72-73	0.21161544281555256
74-75	0.20994141190756122
76-77	0.2039178899796994
78-79	0.22230233655853326
80-81	0.23372161882376014
82-83	0.23277997643801496
84-85	0.2268311880328313
86-87	0.246994292451406
88-89	0.23952094018358738
90-91	0.2461871704064816
92-93	0.24152379859136278
94-95	0.24491670052095246
96-97	0.2518370247209525
98-99	0.24515584779352265
100-101	0.2519864917663089
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3345219.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.6423532817819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.9990155751069	44.96046811411521
2	11.80629272858157	13.610849928180633
3	4.051019859189829	7.005309536248035
4	1.89530557235182	4.369994935137338
5	1.0878999634705384	3.13545570148032
6	0.680231250143645	2.3526078020452883
7	0.4624862465696991	1.866115692891512
8	0.32798563987480867	1.5124691300012012
9	0.2412269166010663	1.2514398433014244
>10	1.3537301632798502	13.751947976296572
>50	0.06623590401727662	2.582886362187458
>100	0.026539261867036222	2.573687799515679
>500	0.0014060208107926184	0.5741683512370833
>1k	6.248981351909262E-4	0.452598827362323
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.9893409071274555E-5	0.0
4	0.0	0.0	0.0	2.9893409071274555E-5	0.0
5	0.0	2.9893409071274555E-5	0.0	2.9893409071274555E-5	0.0
6	0.0	2.9893409071274555E-5	0.0	5.978681814254911E-5	0.0
7	0.0	2.9893409071274555E-5	0.0	5.978681814254911E-5	0.0
8	0.0	2.9893409071274555E-5	0.0	5.978681814254911E-5	0.0
9	0.0	2.9893409071274555E-5	0.0	5.978681814254911E-5	0.0
10-11	0.0	2.9893409071274555E-5	0.0	5.978681814254911E-5	0.0
12-13	0.0	2.9893409071274555E-5	0.0	5.978681814254911E-5	0.0
14-15	0.0	2.9893409071274555E-5	0.0	7.473352267818638E-5	0.0
16-17	0.0	2.9893409071274555E-5	0.0	8.968022721382367E-5	7.473352267818638E-5
18-19	0.0	4.484011360691183E-5	0.0	1.0462693174946095E-4	1.1957363628509822E-4
20-21	0.0	5.978681814254911E-5	0.0	1.494670453563728E-4	1.3452034082073552E-4
22-23	0.0	5.978681814254911E-5	0.0	1.7936045442764733E-4	1.6441374989201006E-4
24-25	0.0	5.978681814254911E-5	0.0	3.5872090885529466E-4	1.7936045442764733E-4
26-27	0.0	5.978681814254911E-5	0.0	7.174418177105893E-4	1.7936045442764733E-4
28-29	0.0	5.978681814254911E-5	0.0	0.0030939678388769167	1.7936045442764733E-4
30-31	0.0	5.978681814254911E-5	0.0	0.013750968172786296	1.7936045442764733E-4
32-33	0.0	5.978681814254911E-5	0.0	0.04162657213174982	2.2420056803455917E-4
34-35	0.0	5.978681814254911E-5	0.0	0.07688584813131816	2.3914727257019644E-4
36-37	0.0	5.978681814254911E-5	0.0	0.13069398445961236	2.3914727257019644E-4
38-39	0.0	5.978681814254911E-5	0.0	0.2230048316717082	2.540939771058337E-4
40-41	0.0	5.978681814254911E-5	2.9893409071274555E-5	0.3312189725097221	2.69040681641471E-4
42-43	0.0	5.978681814254911E-5	2.9893409071274555E-5	0.44294558891361074	2.69040681641471E-4
44-45	0.0	7.473352267818638E-5	2.9893409071274555E-5	0.5802908568915817	2.69040681641471E-4
46-47	0.0	8.968022721382367E-5	2.9893409071274555E-5	0.7396974607641533	2.69040681641471E-4
48-49	0.0	8.968022721382367E-5	2.9893409071274555E-5	0.9051873733827291	3.288274997840201E-4
50-51	0.0	8.968022721382367E-5	2.9893409071274555E-5	1.0774630898604844	3.288274997840201E-4
52-53	0.0	8.968022721382367E-5	2.9893409071274555E-5	1.2518014515641576	4.932412496760302E-4
54-55	0.0	8.968022721382367E-5	2.9893409071274555E-5	1.4546880189308982	5.978681814254912E-4
56-57	0.0	8.968022721382367E-5	2.9893409071274555E-5	1.6865861398013104	5.978681814254912E-4
58-59	0.0	8.968022721382367E-5	2.9893409071274555E-5	1.9313533732769064	5.978681814254912E-4
60-61	0.0	8.968022721382367E-5	2.9893409071274555E-5	2.1926815553779884	5.978681814254912E-4
62-63	0.0	8.968022721382367E-5	2.9893409071274555E-5	2.464053922927019	5.978681814254912E-4
64-65	0.0	8.968022721382367E-5	2.9893409071274555E-5	2.74892316467173	5.978681814254912E-4
66-67	0.0	8.968022721382367E-5	2.9893409071274555E-5	3.0760616868432233	6.277615904967658E-4
68-69	0.0	8.968022721382367E-5	2.9893409071274555E-5	3.429655876042794	6.576549995680402E-4
70-71	0.0	8.968022721382367E-5	2.9893409071274555E-5	3.811364816473899	6.576549995680402E-4
72-73	0.0	8.968022721382367E-5	2.9893409071274555E-5	4.199246745878222	7.174418177105893E-4
74-75	0.0	8.968022721382367E-5	2.9893409071274555E-5	4.602628407886001	7.174418177105893E-4
76-77	0.0	8.968022721382367E-5	2.9893409071274555E-5	5.053794684294212	7.174418177105893E-4
78-79	0.0	8.968022721382367E-5	2.9893409071274555E-5	5.54968150067305	7.323885222462266E-4
80-81	0.0	8.968022721382367E-5	2.9893409071274555E-5	6.073040359988389	7.772286358531384E-4
82-83	0.0	8.968022721382367E-5	2.9893409071274555E-5	6.6062042574791064	8.07122044924413E-4
84-85	0.0	8.968022721382367E-5	2.9893409071274555E-5	7.164359044953409	8.370154539956876E-4
86-87	0.0	8.968022721382367E-5	2.9893409071274555E-5	7.7576834282000675	8.370154539956876E-4
88-89	0.0	8.968022721382367E-5	2.9893409071274555E-5	8.403156863571564	8.370154539956876E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2270	0.0	45.78883	1
GTATCAA	4265	0.0	42.954582	1
TCAACGC	4845	0.0	37.32153	4
CAACGCA	5030	0.0	35.66474	5
ATCAACG	5235	0.0	34.45202	3
AACGCAG	5330	0.0	33.81671	6
TATCAAC	5540	0.0	32.727615	2
GTACATG	9020	0.0	28.83469	1
TACATGG	9380	0.0	27.425669	2
ACGCAGA	6560	0.0	26.973972	7
ACATGGG	9650	0.0	25.919765	3
CGCAGAG	6820	0.0	25.668169	8
CATGGGG	8245	0.0	23.312702	4
GAGTACA	6470	0.0	21.273306	1
GCAGAGT	7960	0.0	20.741425	9
AGAGTAC	6150	0.0	19.78751	10-11
AGTACAT	6950	0.0	18.507393	2
GTATATG	1065	0.0	17.825907	1
GTGTAAG	1655	0.0	17.78013	1
ATGGGGG	4925	0.0	17.0562	5
>>END_MODULE