FastQCFastQC Report
Mon 15 Aug 2016
SRR2921676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3345219
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.046.320771
GTATCAA41550.043.249151
TCAACGC50900.034.7771534
CAACGCA53400.033.1777765
ATCAACG54750.032.2449533
TATCAAC55550.032.0368772
AACGCAG56500.031.522716
GTACATG91200.027.0538651
TACATGG93250.026.1587642
ACGCAGA68450.025.5333987
ACATGGG100100.024.036813
CGTTATA1201.722239E-423.7286472
CGCAGAG72850.023.3392948
GTATAGC8700.021.3109191
CATGGGG86250.021.0187664
GTATATA10000.019.0158961
AGAGTAC69700.018.1592810-11
GCAGAGT87750.018.0231789
GTATAGG9350.017.7956261
ATGGGGG48050.017.5956925
Produced by FastQC (version 0.11.5)