##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921676_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3345219
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45012508897026	33.0	31.0	34.0	29.0	34.0
2	31.5826336033605	34.0	31.0	34.0	29.0	34.0
3	31.65765440169986	34.0	31.0	34.0	28.0	34.0
4	35.136689705517036	37.0	35.0	37.0	33.0	37.0
5	34.88313620124721	37.0	35.0	37.0	32.0	37.0
6	34.86122074518888	37.0	35.0	37.0	32.0	37.0
7	34.74488396723802	37.0	35.0	37.0	32.0	37.0
8	34.68981223650828	37.0	35.0	37.0	32.0	37.0
9	36.28804362285399	39.0	37.0	39.0	32.0	39.0
10-11	36.17892416012225	39.0	37.0	39.0	32.0	39.0
12-13	36.104257897614474	39.0	37.0	39.0	32.0	39.0
14-15	37.35484477996806	40.0	38.0	41.0	32.0	41.0
16-17	37.320853133980165	40.0	38.0	41.0	32.0	41.0
18-19	37.27942251314488	40.0	38.0	41.0	31.0	41.0
20-21	37.219222867023056	40.0	38.0	41.0	31.0	41.0
22-23	37.132434827136876	40.0	38.0	41.0	31.0	41.0
24-25	37.04769747511299	40.0	37.5	41.0	31.0	41.0
26-27	36.920790387714526	40.0	37.0	41.0	30.0	41.0
28-29	36.80327282010535	40.0	37.0	41.0	30.0	41.0
30-31	36.651757029958276	40.0	37.0	41.0	30.0	41.0
32-33	36.61032865112867	40.0	37.0	41.0	30.0	41.0
34-35	36.74667951485389	40.0	37.0	41.0	30.0	41.0
36-37	36.74659685957781	40.0	37.0	41.0	30.0	41.0
38-39	36.641646182208106	40.0	37.0	41.0	30.0	41.0
40-41	36.5620189589979	40.0	37.0	41.0	30.0	41.0
42-43	36.448186949793126	40.0	37.0	41.0	30.0	41.0
44-45	36.32256049603927	40.0	36.5	41.0	29.0	41.0
46-47	36.17025671562908	40.0	36.0	41.0	28.5	41.0
48-49	36.02877793651178	40.0	36.0	41.0	28.0	41.0
50-51	35.86916701118821	40.0	35.0	41.0	28.0	41.0
52-53	35.697034484139905	39.0	35.0	41.0	28.0	41.0
54-55	35.482349586080915	39.0	35.0	41.0	27.0	41.0
56-57	35.24909759271366	39.0	35.0	41.0	27.0	41.0
58-59	35.000717591284754	38.5	35.0	40.5	26.0	41.0
60-61	34.71755855147302	38.0	34.5	40.0	26.0	41.0
62-63	34.408436786948776	37.5	34.0	40.0	26.0	41.0
64-65	34.069131348351185	37.0	34.0	40.0	26.0	41.0
66-67	33.71100950341368	36.5	34.0	39.0	25.0	41.0
68-69	33.34455232975778	36.0	34.0	39.0	24.0	41.0
70-71	32.94392967994024	35.0	33.0	38.5	23.0	40.0
72-73	32.577792814162535	35.0	33.0	37.5	22.5	40.0
74-75	32.180990691491346	35.0	33.0	37.0	21.5	39.0
76-77	29.4790604142808	33.0	29.0	34.5	16.5	36.5
78-79	31.04893192344059	34.5	31.5	36.0	19.0	37.0
80-81	31.22812871145357	35.0	32.0	35.5	20.0	37.0
82-83	31.056018454995026	35.0	32.0	35.0	19.0	37.0
84-85	30.863661093638413	35.0	32.0	35.0	18.0	36.0
86-87	30.65769072219188	35.0	32.0	35.0	16.5	36.0
88-89	30.390781291150148	35.0	32.0	35.0	10.0	36.0
90-91	30.195045227233255	34.5	32.0	35.0	4.5	35.0
92-93	30.034532417758	34.0	31.0	35.0	2.0	35.0
94-95	29.930012803347104	34.0	31.0	35.0	2.0	35.0
96-97	29.728774558556555	34.0	31.0	35.0	2.0	35.0
98-99	29.52856419863692	34.0	31.0	35.0	2.0	35.0
100-101	28.404492501088868	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57245.0
3	20506.0
4	11262.0
5	8155.0
6	6979.0
7	7003.0
8	7402.0
9	8008.0
10	8801.0
11	9503.0
12	10131.0
13	10811.0
14	11485.0
15	12510.0
16	13138.0
17	13925.0
18	13569.0
19	12484.0
20	11979.0
21	12061.0
22	12613.0
23	14432.0
24	17227.0
25	20419.0
26	24408.0
27	29733.0
28	35518.0
29	43168.0
30	52185.0
31	65383.0
32	83189.0
33	109450.0
34	156780.0
35	233769.0
36	411945.0
37	811101.0
38	849312.0
39	107628.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.68830433820467	18.89953163212475	13.054111297334995	26.35805273233559
2	15.166841443830764	20.343165096860826	37.955561560821856	26.534431898486556
3	17.943943674604242	24.20625738074951	28.985096491621448	28.864702453024798
4	13.047365961763195	16.237832201344276	35.10269216982855	35.612109667063976
5	14.102257039287608	37.42783696738696	34.35311692571651	14.116789067608918
6	34.454348996502695	35.34148383735144	16.392563444819082	13.81160372132678
7	29.38113450304285	31.57925731596566	20.687381211030193	18.3522269699613
8	26.786377812633493	35.49325171236921	19.338584409570796	18.38178606542651
9	26.848026047928713	15.152584973535358	18.786189719175255	39.21319925936068
10-11	25.9241330672658	25.125182741979092	27.751807081894725	21.198877108860383
12-13	26.263441993275755	22.42196073367059	27.62546532994819	23.689131943105465
14-15	23.313047124143978	24.09919440605851	24.513553245506976	28.074205224290537
16-17	23.827980145392598	27.286453784707938	25.30064926999537	23.5849167999041
18-19	23.925079342113268	26.656125085589938	26.502754497497953	22.91604107479884
20-21	24.34966310769568	25.772324804268553	26.40932545759106	23.46868663044471
22-23	24.184933781614895	25.606544743408428	26.17419068826286	24.034330786713813
24-25	23.99145881430813	25.892374842423614	26.127577309956973	23.988589033311282
26-27	23.817852952839676	26.023280845281494	26.168537631268084	23.990328570610746
28-29	24.02697075557308	26.240851914504216	25.963784186721167	23.768393143201536
30-31	24.24642746785427	25.93564680804562	25.82612867140269	23.991797052697418
32-33	23.92012156064616	26.061364806757886	25.80063426195482	24.21787937064113
34-35	24.208802551360176	26.022767615902804	25.9646489824381	23.803780850298928
36-37	24.27069966346021	25.924221373378302	25.945596096945582	23.859482866215902
38-39	24.181540879685308	25.987162574408433	25.88110075902355	23.950195786882713
40-41	24.24809240118818	25.81730906486292	25.93080314136298	24.003795392585918
42-43	24.05721312195861	25.821034250083034	25.882829874881054	24.2389227530773
44-45	24.01774897178087	25.82237724711564	25.99019576335601	24.169678017747476
46-47	24.187656777496205	25.868335452348756	25.811401548590222	24.132606221564814
48-49	23.935110258478826	25.96401335690927	25.870349368470634	24.230527016141266
50-51	24.01090063410158	25.96432451807129	25.907555364498176	24.117219483328956
52-53	24.22131178170154	25.896979054719598	25.847594050482115	24.03411511309674
54-55	23.718614161487046	25.75741473408928	26.12973772372226	24.394233380701415
56-57	23.80823187365935	25.85343468089718	25.965514921859235	24.372818523584236
58-59	23.884265991848604	25.796632527123474	25.990102562140027	24.32899891888789
60-61	23.918183909520145	25.763180517108474	26.089402117899276	24.229233455472112
62-63	23.9084210307575	25.76804046881127	26.103815471453668	24.219723028977562
64-65	23.628232545606956	25.6365804255762	26.095049917116324	24.64013711170052
66-67	23.634342696824984	25.884820124076498	25.923510837512616	24.557326341585906
68-69	23.77352017878207	25.676160276978223	25.95139482077528	24.598924723464428
70-71	23.70072703167422	25.522853156593	25.976380444810328	24.80003936692245
72-73	23.642672765418926	25.69932704798222	25.83331040719219	24.824689779406665
74-75	23.55668988720839	25.809277791017514	25.85726976783545	24.776762553938646
76-77	23.540816042192073	25.44191024476711	26.05776061655749	24.959513096483324
78-79	23.491648038019346	25.450120628064788	26.045025601393775	25.013205732522092
80-81	23.594796766158847	25.618093787854747	25.823690056669847	24.963419389316563
82-83	23.488440310570784	25.682550636995433	25.870019202857165	24.95898984957662
84-85	23.43660833601518	25.589044070408328	25.930186859871245	25.044160733705244
86-87	23.34847317985686	25.692245264616272	25.835920997192748	25.123360558334127
88-89	23.230213659646616	25.502354258577192	25.926255717003276	25.341176364772917
90-91	23.15065381890959	25.4412574910894	25.93737280608057	25.47071588392044
92-93	23.238815150645504	25.371108503316464	26.041878682913822	25.34819766312421
94-95	23.08042784633587	25.20752161691295	26.134203117140537	25.577847419610645
96-97	22.96020737192855	25.11071944844329	26.15420147904978	25.774871700578377
98-99	22.830700990260024	25.086377960900187	26.148158754889867	25.934762293949927
100-101	22.784285666669707	25.048027577313075	26.16004927637653	26.00763747964069
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	24.0
2	40.5
3	57.5
4	59.5
5	66.5
6	104.5
7	237.5
8	354.5
9	398.5
10	358.0
11	294.0
12	321.5
13	355.0
14	380.5
15	419.5
16	454.5
17	530.0
18	628.0
19	679.5
20	776.5
21	913.5
22	1055.5
23	1304.0
24	1570.5
25	2017.5
26	2799.5
27	3945.5
28	5895.0
29	8674.0
30	12579.0
31	18136.0
32	26327.0
33	32812.0
34	37259.5
35	43921.0
36	52917.0
37	62270.0
38	71064.0
39	79708.5
40	88005.5
41	95789.5
42	103214.0
43	112411.5
44	122398.0
45	132020.5
46	138186.0
47	143025.5
48	149152.5
49	150997.0
50	150285.0
51	149670.0
52	148650.5
53	148168.0
54	146133.5
55	139347.0
56	128485.0
57	116001.0
58	104731.5
59	92475.5
60	75896.0
61	60674.0
62	48983.0
63	36717.0
64	25619.5
65	18257.5
66	13410.5
67	10187.0
68	7532.0
69	5349.0
70	3794.5
71	2677.5
72	1821.5
73	1243.0
74	842.5
75	540.0
76	327.5
77	179.5
78	103.5
79	53.5
80	44.5
81	39.5
82	18.5
83	10.0
84	8.5
85	6.5
86	5.5
87	3.0
88	0.5
89	1.0
90	1.5
91	1.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1718871021598287
2	2.092538634989219E-4
3	0.011030667947300312
4	0.006636336813822951
5	0.02636598680086416
6	0.00203275181684667
7	2.9893409071274555E-5
8	0.0
9	2.9893409071274555E-5
10-11	0.05102804928466567
12-13	3.5872090885529466E-4
14-15	0.005126719655723587
16-17	1.4946704535637278E-5
18-19	0.0015992973853131886
20-21	3.138807952483829E-4
22-23	0.0
24-25	4.782945451403929E-4
26-27	0.003243434884233289
28-29	0.005634907609935254
30-31	0.01696450964794831
32-33	0.011553802606047615
34-35	0.010059132152483889
36-37	0.0043793844289417225
38-39	0.0
40-41	0.0033032217023758383
42-43	0.009625677720950406
44-45	0.007069791245356433
46-47	0.0033480618159827504
48-49	0.008474781471706336
50-51	0.0027651403390928963
52-53	0.0022121122712743173
54-55	0.0041701305654428
56-57	0.00878866226695472
58-59	0.015395105671706398
60-61	0.0075779791995681
62-63	0.021448521008639494
64-65	0.04113333088207379
66-67	0.06096760780086445
68-69	0.05102804928466567
70-71	0.0690836683637155
72-73	0.034287740204751914
74-75	0.027203002254859845
76-77	0.23077711803023956
78-79	0.08730370119265735
80-81	0.06754415779654487
82-83	0.027651403390928964
84-85	0.05234335928380175
86-87	0.06799255893261398
88-89	0.29217818026263753
90-91	0.29912839787170886
92-93	0.05451063144146915
94-95	0.009760198061771143
96-97	0.015066278171922375
98-99	0.0011060561356371586
100-101	1.6441374989201006E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3345219.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.13008447353541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.72075882255457	43.063426735837254
2	12.518172419308037	14.052921507000828
3	4.248030758648182	7.153269759872975
4	1.9971746954832916	4.484063374635381
5	1.1300955078105452	3.1716178158284403
6	0.7184906951097179	2.419736604597458
7	0.49649029636156694	1.9507629592545772
8	0.3671524377957523	1.648663787851203
9	0.2693207131762496	1.3605294942950126
>10	1.4312935146640737	14.198560613310542
>50	0.07278854708202097	2.7722290597218278
>100	0.028093544171275936	2.6732711764212445
>500	0.0015500846818745741	0.626745987106913
>1k	5.87963152847534E-4	0.4242011242662835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9893409071274555E-5
2	0.0	0.0	0.0	2.9893409071274555E-5	2.9893409071274555E-5
3	0.0	0.0	0.0	2.9893409071274555E-5	2.9893409071274555E-5
4	0.0	0.0	0.0	2.9893409071274555E-5	2.9893409071274555E-5
5	0.0	0.0	0.0	2.9893409071274555E-5	2.9893409071274555E-5
6	0.0	0.0	0.0	5.978681814254911E-5	2.9893409071274555E-5
7	0.0	0.0	0.0	8.968022721382367E-5	2.9893409071274555E-5
8	0.0	0.0	0.0	8.968022721382367E-5	2.9893409071274555E-5
9	0.0	0.0	0.0	8.968022721382367E-5	2.9893409071274555E-5
10-11	0.0	0.0	0.0	8.968022721382367E-5	2.9893409071274555E-5
12-13	1.4946704535637278E-5	0.0	0.0	8.968022721382367E-5	8.968022721382367E-5
14-15	2.9893409071274555E-5	0.0	0.0	1.0462693174946095E-4	1.0462693174946095E-4
16-17	2.9893409071274555E-5	0.0	0.0	1.6441374989201006E-4	1.1957363628509822E-4
18-19	2.9893409071274555E-5	0.0	0.0	1.943071589632846E-4	1.7936045442764733E-4
20-21	2.9893409071274555E-5	0.0	0.0	2.3914727257019644E-4	1.7936045442764733E-4
22-23	2.9893409071274555E-5	0.0	0.0	2.69040681641471E-4	1.943071589632846E-4
24-25	2.9893409071274555E-5	0.0	0.0	4.185077269978438E-4	2.092538634989219E-4
26-27	2.9893409071274555E-5	0.0	0.0	8.071220449244131E-4	2.3914727257019644E-4
28-29	2.9893409071274555E-5	0.0	0.0	0.0032284881796976524	2.3914727257019644E-4
30-31	2.9893409071274555E-5	0.0	0.0	0.014124635786177227	2.540939771058337E-4
32-33	2.9893409071274555E-5	0.0	0.0	0.042657894744708796	2.69040681641471E-4
34-35	2.9893409071274555E-5	0.0	0.0	0.07887375983455791	2.69040681641471E-4
36-37	2.9893409071274555E-5	0.0	0.0	0.13410183309373766	2.69040681641471E-4
38-39	2.9893409071274555E-5	0.0	0.0	0.22857995246350088	2.69040681641471E-4
40-41	2.9893409071274555E-5	0.0	0.0	0.3383485505732211	2.69040681641471E-4
42-43	2.9893409071274555E-5	0.0	0.0	0.4512260632263538	2.69040681641471E-4
44-45	2.9893409071274555E-5	0.0	0.0	0.5900958950669597	2.839873861771083E-4
46-47	2.9893409071274555E-5	0.0	0.0	0.7519836518924471	2.989340907127456E-4
48-49	2.9893409071274555E-5	0.0	0.0	0.9188486613283017	2.989340907127456E-4
50-51	2.9893409071274555E-5	0.0	0.0	1.0928731422367264	2.989340907127456E-4
52-53	2.9893409071274555E-5	0.0	0.0	1.2690051084846763	2.989340907127456E-4
54-55	2.9893409071274555E-5	0.0	0.0	1.4732219325550884	2.989340907127456E-4
56-57	2.9893409071274555E-5	0.0	0.0	1.7064802035382436	3.288274997840201E-4
58-59	2.9893409071274555E-5	0.0	0.0	1.9535641762168634	3.5872090885529466E-4
60-61	2.9893409071274555E-5	0.0	0.0	2.2178219124069303	3.8861431792656926E-4
62-63	2.9893409071274555E-5	0.0	0.0	2.4903302295006693	4.185077269978438E-4
64-65	2.9893409071274555E-5	0.0	0.0	2.7769631823805856	4.4840113606911834E-4
66-67	2.9893409071274555E-5	0.0	0.0	3.10659780420953	4.782945451403929E-4
68-69	2.9893409071274555E-5	0.0	0.0	3.463031867270872	4.782945451403929E-4
70-71	2.9893409071274555E-5	0.0	0.0	3.8475507881546767	4.782945451403929E-4
72-73	2.9893409071274555E-5	0.0	0.0	4.240634170737401	5.679747723542166E-4
74-75	2.9893409071274555E-5	0.0	0.0	4.648858565014727	5.679747723542166E-4
76-77	2.9893409071274555E-5	0.0	0.0	5.104493906079094	5.679747723542166E-4
78-79	2.9893409071274555E-5	0.0	0.0	5.605537335522727	5.679747723542166E-4
80-81	2.9893409071274555E-5	0.0	0.0	6.134516155743466	5.679747723542166E-4
82-83	2.9893409071274555E-5	0.0	0.0	6.676095047887747	5.679747723542166E-4
84-85	2.9893409071274555E-5	0.0	0.0	7.242350949220365	5.679747723542166E-4
86-87	2.9893409071274555E-5	0.0	0.0	7.843701712802659	5.679747723542166E-4
88-89	2.9893409071274555E-5	0.0	0.0	8.5002656029396	5.978681814254912E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2340	0.0	46.32077	1
GTATCAA	4155	0.0	43.24915	1
TCAACGC	5090	0.0	34.777153	4
CAACGCA	5340	0.0	33.177776	5
ATCAACG	5475	0.0	32.244953	3
TATCAAC	5555	0.0	32.036877	2
AACGCAG	5650	0.0	31.52271	6
GTACATG	9120	0.0	27.053865	1
TACATGG	9325	0.0	26.158764	2
ACGCAGA	6845	0.0	25.533398	7
ACATGGG	10010	0.0	24.03681	3
CGTTATA	120	1.722239E-4	23.728647	2
CGCAGAG	7285	0.0	23.339294	8
GTATAGC	870	0.0	21.310919	1
CATGGGG	8625	0.0	21.018766	4
GTATATA	1000	0.0	19.015896	1
AGAGTAC	6970	0.0	18.15928	10-11
GCAGAGT	8775	0.0	18.023178	9
GTATAGG	935	0.0	17.795626	1
ATGGGGG	4805	0.0	17.595692	5
>>END_MODULE