FastQCFastQC Report
Mon 15 Aug 2016
SRR2921675_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921675_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826058
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27300.054.0578731
GTATCAA49150.042.9633061
TCAACGC60500.033.7327084
CAACGCA61450.033.055575
ATCAACG63400.032.1874473
TATCAAC63700.032.035292
AACGCAG64950.031.3279326
GTACATG86950.028.9791831
TACATGG89700.026.9821992
ACGCAGA75050.026.1685777
ACATGGG92050.025.6751443
CGCAGAG76550.025.3486428
CATGGGG73300.023.1801994
GCAGAGT87850.021.063119
GTATAGG7150.019.9102361
AGAGTAC71850.019.21663910-11
CAGAGTA78400.019.03337710-11
GAGTACA66250.018.766211
GTGTACG3558.0035534E-1118.7137531
GTATATA9850.018.3066371
Produced by FastQC (version 0.11.5)