##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921675_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2826058
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.85730936873907	31.0	31.0	34.0	25.0	34.0
2	29.9152533316726	31.0	31.0	34.0	25.0	34.0
3	29.96690443012847	31.0	31.0	34.0	25.0	34.0
4	33.407824255553145	37.0	35.0	37.0	28.0	37.0
5	33.405619063727634	37.0	35.0	37.0	27.0	37.0
6	33.44103411890343	37.0	35.0	37.0	28.0	37.0
7	33.342823112618355	37.0	35.0	37.0	27.0	37.0
8	33.30707756174856	37.0	35.0	37.0	27.0	37.0
9	34.80477329198481	39.0	35.0	39.0	27.0	39.0
10-11	34.69200313652445	39.0	35.0	39.0	27.0	39.0
12-13	34.57201922253542	39.0	35.0	39.0	27.0	39.0
14-15	35.63894920061796	40.0	36.0	41.0	27.0	41.0
16-17	35.57541175729585	40.0	36.0	41.0	26.0	41.0
18-19	35.546518684329904	40.0	36.0	41.0	26.0	41.0
20-21	35.46832672931695	40.0	36.0	41.0	25.0	41.0
22-23	35.36427897091992	40.0	36.0	41.0	25.0	41.0
24-25	35.24419792516643	40.0	35.5	41.0	24.5	41.0
26-27	35.0941564185873	39.5	35.0	41.0	24.0	41.0
28-29	34.95040229181425	39.0	35.0	41.0	23.0	41.0
30-31	34.79052305366698	39.0	35.0	41.0	22.5	41.0
32-33	34.60404793532192	39.0	34.5	41.0	21.0	41.0
34-35	34.425015516312826	39.0	34.0	41.0	19.0	41.0
36-37	34.380072171201014	39.0	34.0	41.0	18.5	41.0
38-39	34.553408670310375	39.0	35.0	41.0	19.5	41.0
40-41	34.606953218936056	39.0	35.0	41.0	18.5	41.0
42-43	34.445568526902136	39.0	35.0	41.0	16.0	41.0
44-45	34.316750399319474	39.0	34.5	41.0	15.0	41.0
46-47	34.14800403954908	39.0	34.0	41.0	13.0	41.0
48-49	33.975983507769485	39.0	34.0	41.0	9.0	41.0
50-51	32.312958545082935	37.0	32.0	39.5	7.5	40.0
52-53	32.42576620861992	37.5	32.0	39.5	7.0	40.0
54-55	33.32712102865547	38.0	33.0	40.0	4.0	41.0
56-57	33.35480464307527	38.0	33.0	41.0	2.0	41.0
58-59	33.17238393550309	38.0	33.0	40.0	2.0	41.0
60-61	32.92666941018196	37.5	33.0	40.0	2.0	41.0
62-63	32.63950209089835	37.0	32.5	40.0	2.0	41.0
64-65	32.36154636599815	36.5	32.0	40.0	2.0	41.0
66-67	32.02285816497751	36.0	32.0	39.0	2.0	41.0
68-69	31.664190013085367	35.5	32.0	39.0	2.0	41.0
70-71	31.255605157431305	35.0	31.0	39.0	2.0	40.5
72-73	30.89026021405081	35.0	31.0	37.5	2.0	40.0
74-75	30.495688340437457	35.0	31.0	37.0	2.0	39.0
76-77	30.11704607619518	35.0	31.0	36.5	2.0	39.0
78-79	29.76304856446683	35.0	30.0	36.0	2.0	38.5
80-81	29.44235840170301	35.0	30.0	36.0	2.0	37.0
82-83	29.125268837369934	35.0	30.0	35.0	2.0	37.0
84-85	28.851826289481675	34.5	29.5	35.0	2.0	36.5
86-87	28.608609412828752	34.0	29.0	35.0	2.0	36.0
88-89	28.373699513598094	34.0	29.0	35.0	2.0	36.0
90-91	28.14023827536448	34.0	29.0	35.0	2.0	35.5
92-93	27.936127460936753	34.0	29.0	35.0	2.0	35.0
94-95	27.69080677042014	34.0	29.0	35.0	2.0	35.0
96-97	27.43660887356169	34.0	28.5	35.0	2.0	35.0
98-99	27.150740182968644	34.0	27.0	35.0	2.0	35.0
100-101	26.069712298898324	33.0	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	124662.0
3	20305.0
4	13630.0
5	10156.0
6	9030.0
7	9449.0
8	9810.0
9	10520.0
10	11521.0
11	12174.0
12	12146.0
13	12456.0
14	13078.0
15	13960.0
16	14271.0
17	15071.0
18	14152.0
19	13177.0
20	13305.0
21	14089.0
22	15486.0
23	17702.0
24	20758.0
25	23973.0
26	28569.0
27	33352.0
28	39332.0
29	45549.0
30	54057.0
31	65511.0
32	80391.0
33	103146.0
34	140890.0
35	201276.0
36	326292.0
37	565737.0
38	591888.0
39	105179.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.08993512333082	18.24973198188948	12.902682163801908	24.757650730977794
2	15.901048808404067	19.674860895481793	39.75219705812359	24.67189323799055
3	19.05926963894853	23.904392910349586	29.63804197824916	27.398295472452727
4	13.134592523263214	15.701912157001061	37.01720222431627	34.14629309541945
5	13.936955761951857	37.45136803354937	34.67981956199272	13.931856642506046
6	33.18048965889921	35.50839189273835	17.245218371108972	14.06590007725347
7	28.711207622649948	32.06253777846355	21.11315348779269	18.113101111093812
8	26.432875533260518	34.9025425918284	20.112778362523013	18.551803512388066
9	26.93929375095324	15.252839197037957	19.50527391930392	38.302593132704885
10-11	25.53844727375872	25.185772913526215	28.233377670516045	21.042402142199023
12-13	26.028333978086415	22.86167020189561	27.812400566155738	23.297595253862234
14-15	23.23969763006198	24.48227962634926	25.142554526132756	27.135468217456012
16-17	23.3388012734419	27.44706542574099	26.01006315709742	23.204070143719694
18-19	23.32617185841159	26.878781272914125	27.23540998411759	22.559636884556696
20-21	23.99000668556841	26.032661956921437	27.16246639077342	22.81486496673673
22-23	23.859713523930353	25.877538276810757	26.764545754470525	23.49820244478836
24-25	23.525014123673827	26.062479026875334	26.855599417212616	23.556907432238226
26-27	23.379270912800358	26.291694693031893	26.75144210801445	23.577592286153305
28-29	23.511720444795817	26.505956303220458	26.641230315817488	23.341092936166238
30-31	23.867624641577777	26.165561208916916	26.478893675224562	23.48792047428074
32-33	23.573397644594174	26.303329275542847	26.331462503184895	23.791810576678085
34-35	23.959620337039787	26.284750758821662	26.41515042406431	23.340478480074243
36-37	23.936510419090183	26.099715202039885	26.50130208771041	23.462472291159514
38-39	23.768576014630092	26.1718689179874	26.522139764384157	23.537415302998347
40-41	24.001031836727762	26.032752323623065	26.525227894906227	23.44098794474295
42-43	23.754466150371258	26.03950342704157	26.320619270712786	23.885411151874386
44-45	23.656798585833155	26.04336471001246	26.53562810390651	23.76420860024787
46-47	23.84481185110792	26.03219421427595	26.271231370271053	23.851762564345076
48-49	23.609784850537007	26.172253275191252	26.32243178173702	23.895530092534713
50-51	23.72702633024494	26.26065059453962	26.35765713339383	23.65466594182161
52-53	23.900959184817033	26.04481491910801	26.359619475360024	23.69460642071493
54-55	23.355067758423743	25.930873747294577	26.697914778971676	24.016143715310005
56-57	23.50712431672914	26.060543836261118	26.50298310924993	23.929348737759817
58-59	23.567324026387553	25.9078457372052	26.608500176719772	23.91633005968748
60-61	23.550941889766825	25.988953662524334	26.676995451539394	23.783108996169446
62-63	23.555330870227316	25.896055885884756	26.720970612246635	23.82764263164129
64-65	23.374637844474428	25.77220982651467	26.672768865733946	24.180383463276954
66-67	23.343260642215792	25.909957015154454	26.553630341676502	24.19315200095325
68-69	23.491124165931883	25.808066238567996	26.61069362516904	24.090115970331087
70-71	23.421401519516607	25.60618089459794	26.60790450738395	24.364513078501503
72-73	23.314058165237107	25.80526075202944	26.528824272668565	24.351856810064888
74-75	23.323321175713367	25.86565859488174	26.54878444486605	24.26223578453884
76-77	23.26129577536707	25.56612293948129	26.67172085558577	24.500860429565872
78-79	23.235116837830443	25.541549679286263	26.750102542213433	24.473230940669865
80-81	23.406934099118505	25.691644366046685	26.556894824370314	24.3445267104645
82-83	23.351967731826406	25.64688781425263	26.648981102588152	24.352163351332813
84-85	23.304739304638574	25.681157220221312	26.62459878595232	24.389504689187795
86-87	23.20801344395673	25.69986018937759	26.685949014709383	24.406177351956302
88-89	23.12338309834274	25.430100718362823	26.789442113280042	24.657074070014392
90-91	23.131088953801466	25.395535884695587	26.816584445909196	24.65679071559375
92-93	23.22931925880638	25.25303800806919	26.938639624390763	24.579003108733666
94-95	23.132361893328905	25.138209547810714	26.981618413939014	24.747810144921367
96-97	23.054606385482902	24.974185707866827	27.096713881854	24.874494024796267
98-99	22.93905621177455	24.88542897021854	27.052474125846587	25.12304069216032
100-101	23.105493237667552	24.79447987052196	27.187247889276694	24.912779002533792
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	43.5
2	60.5
3	85.0
4	80.5
5	112.0
6	185.0
7	362.5
8	556.0
9	617.0
10	573.5
11	551.5
12	613.5
13	681.5
14	754.5
15	860.0
16	939.0
17	990.0
18	1130.0
19	1284.0
20	1374.0
21	1541.0
22	1706.5
23	1881.5
24	2194.5
25	2609.0
26	3246.5
27	4374.0
28	6136.0
29	8765.0
30	12816.5
31	18281.0
32	25986.0
33	32590.0
34	37776.5
35	44606.5
36	53450.5
37	62637.5
38	70219.5
39	77229.5
40	83280.0
41	88368.0
42	93187.5
43	99371.0
44	105681.0
45	112032.5
46	116757.0
47	119877.0
48	123112.5
49	124898.0
50	124816.0
51	122327.0
52	119339.5
53	116875.0
54	113117.0
55	107050.5
56	98111.0
57	87274.5
58	78702.5
59	68838.0
60	56241.5
61	45700.5
62	37348.5
63	28782.0
64	20482.5
65	14600.5
66	10668.5
67	8026.0
68	5894.0
69	4260.0
70	3146.5
71	2229.5
72	1537.5
73	1109.0
74	803.0
75	546.5
76	381.5
77	269.5
78	178.5
79	137.5
80	116.0
81	96.0
82	87.5
83	80.0
84	65.0
85	51.0
86	46.0
87	46.0
88	38.0
89	27.5
90	21.0
91	16.0
92	14.0
93	15.0
94	12.5
95	10.5
96	8.5
97	6.5
98	6.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.057394434226049146
2	0.0807485196694477
3	0.07667924720582521
4	0.07710386694115974
5	0.07222073998481277
6	0.010438568493640258
7	0.013233981751259175
8	0.018258648619384314
9	0.006050831228516895
10-11	0.012862439482841471
12-13	0.024309479847901213
14-15	0.04154196410689377
16-17	0.02836105982255141
18-19	0.010261643603917542
20-21	0.020134052450445108
22-23	0.039277325518443006
24-25	0.03588036763576685
26-27	0.021000984410086417
28-29	0.011040113118697494
30-31	0.010810110762057961
32-33	0.007713925191910428
34-35	0.004617739621762894
36-37	6.900070699185933E-4
38-39	0.0036092677503434113
40-41	0.0010438568493640256
42-43	0.2454125145343797
44-45	0.21312372215998399
46-47	0.219510710678974
48-49	0.2269415560473281
50-51	0.21744068946921827
52-53	0.22547307946262957
54-55	0.22154534691078528
56-57	0.2246592249699051
58-59	0.23433701643773766
60-61	0.23893706357052827
62-63	0.223349980785957
64-65	0.2232615183410956
66-67	0.2203422576606708
68-69	0.21476912363440526
70-71	0.20590518665929716
72-73	0.21920993836644542
74-75	0.2177237692927746
76-77	0.21071754365975504
78-79	0.22956004441522432
80-81	0.24120170215897904
82-83	0.24095400731336722
84-85	0.2351862559084067
86-87	0.25636416520821587
88-89	0.24801331041330363
90-91	0.2556210806713804
92-93	0.24997717668922576
94-95	0.2530025923034842
96-97	0.2606988250064224
98-99	0.25383413928518095
100-101	0.2602918977600601
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2826058.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.48584157718149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.35122037826127	48.78976565133788
2	11.592103449785208	14.255004725195846
3	3.818467237138169	7.043450148310059
4	1.675113578696153	4.119830724939888
5	0.9364141114914993	2.8788104854901744
6	0.5607710005526444	2.0687686140634463
7	0.3793037329802027	1.6325266464958004
8	0.282446837998625	1.389318522812744
9	0.20876164373284262	1.1552296818554608
>10	1.1286958737009267	12.224150781158112
>50	0.04985617005259251	2.056027777720282
>100	0.015345064102646888	1.519846240096999
>500	9.236440045047705E-4	0.4129062747286188
>1k	5.772775027189877E-4	0.4543637257946863
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.538497794454325E-5
7	0.0	0.0	0.0	0.0	3.538497794454325E-5
8	0.0	0.0	0.0	0.0	7.07699558890865E-5
9	0.0	0.0	0.0	3.538497794454325E-5	1.41539911778173E-4
10-11	0.0	0.0	0.0	3.538497794454325E-5	1.41539911778173E-4
12-13	0.0	0.0	0.0	8.846244486135813E-5	1.7692488972271623E-4
14-15	0.0	0.0	0.0	1.0615493383362974E-4	2.1230986766725948E-4
16-17	0.0	0.0	0.0	1.0615493383362974E-4	2.83079823556346E-4
18-19	0.0	0.0	0.0	1.0615493383362974E-4	3.3615729047316084E-4
20-21	0.0	0.0	0.0	1.41539911778173E-4	3.5384977944543245E-4
22-23	0.0	0.0	0.0	2.300023566395311E-4	3.8923475738997574E-4
24-25	0.0	0.0	0.0	4.776972022513338E-4	4.2461973533451896E-4
26-27	0.0	0.0	0.0	9.553944045026677E-4	4.2461973533451896E-4
28-29	0.0	0.0	0.0	0.0035384977944543246	4.2461973533451896E-4
30-31	0.0	0.0	0.0	0.01578170016326629	4.2461973533451896E-4
32-33	0.0	0.0	0.0	0.04465584216601358	4.2461973533451896E-4
34-35	0.0	0.0	0.0	0.08324316061453799	4.600047132790622E-4
36-37	0.0	0.0	0.0	0.14716612327135536	4.600047132790622E-4
38-39	0.0	0.0	0.0	0.2520825828769261	4.600047132790622E-4
40-41	0.0	0.0	0.0	0.37203765810892775	4.600047132790622E-4
42-43	0.0	0.0	0.0	0.4939212146389069	4.776972022513338E-4
44-45	0.0	0.0	0.0	0.6465012395357774	5.130821801958771E-4
46-47	0.0	0.0	0.0	0.8258676927366672	5.484671581404204E-4
48-49	0.0	0.0	0.0	1.0116742119234636	5.66159647112692E-4
50-51	0.0	0.0	0.0	1.2072292925339818	6.369296030017784E-4
52-53	0.0	0.0	0.0	1.4032266853688071	6.5462209197405E-4
54-55	0.0	1.7692488972271625E-5	0.0	1.6286997648314365	6.723145809463217E-4
56-57	0.0	3.538497794454325E-5	0.0	1.8931493974999807	6.723145809463217E-4
58-59	0.0	3.538497794454325E-5	0.0	2.1662329647869933	6.900070699185933E-4
60-61	0.0	3.538497794454325E-5	0.0	2.454532072590159	7.607770258076798E-4
62-63	0.0	3.538497794454325E-5	0.0	2.7466173730333914	8.846244486135812E-4
64-65	0.0	3.538497794454325E-5	0.0	3.0686029798397625	9.023169375858527E-4
66-67	0.0	3.538497794454325E-5	0.0	3.442675274180502	9.200094265581244E-4
68-69	0.0	3.538497794454325E-5	0.0	3.8510002271715584	9.200094265581244E-4
70-71	0.0	3.538497794454325E-5	0.0	4.2756553474840215	9.200094265581244E-4
72-73	0.0	3.538497794454325E-5	0.0	4.709864411841512	9.553944045026677E-4
74-75	0.0	3.538497794454325E-5	0.0	5.166525244704815	9.553944045026677E-4
76-77	0.0	3.538497794454325E-5	0.0	5.67702432151074	9.730868934749393E-4
78-79	0.0	3.538497794454325E-5	0.0	6.239238543582616	9.907793824472108E-4
80-81	0.0	3.538497794454325E-5	0.0	6.822542212509439	9.907793824472108E-4
82-83	0.0	3.538497794454325E-5	0.0	7.428757654655354	0.001132319294225384
84-85	0.0	3.538497794454325E-5	0.0	8.058627954557195	0.001132319294225384
86-87	0.0	3.538497794454325E-5	0.0	8.7347818056105	0.001132319294225384
88-89	0.0	3.538497794454325E-5	0.0	9.460757705609723	0.001132319294225384
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2730	0.0	54.057873	1
GTATCAA	4915	0.0	42.963306	1
TCAACGC	6050	0.0	33.732708	4
CAACGCA	6145	0.0	33.05557	5
ATCAACG	6340	0.0	32.187447	3
TATCAAC	6370	0.0	32.03529	2
AACGCAG	6495	0.0	31.327932	6
GTACATG	8695	0.0	28.979183	1
TACATGG	8970	0.0	26.982199	2
ACGCAGA	7505	0.0	26.168577	7
ACATGGG	9205	0.0	25.675144	3
CGCAGAG	7655	0.0	25.348642	8
CATGGGG	7330	0.0	23.180199	4
GCAGAGT	8785	0.0	21.06311	9
GTATAGG	715	0.0	19.910236	1
AGAGTAC	7185	0.0	19.216639	10-11
CAGAGTA	7840	0.0	19.033377	10-11
GAGTACA	6625	0.0	18.76621	1
GTGTACG	355	8.0035534E-11	18.713753	1
GTATATA	985	0.0	18.306637	1
>>END_MODULE