FastQCFastQC Report
Mon 15 Aug 2016
SRR2921675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826058
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26050.051.4522061
GTATCAA46050.042.0075571
TCAACGC57150.032.5497674
TATCAAC59850.031.3181152
CAACGCA59550.031.2683735
ATCAACG60700.030.801383
AACGCAG64250.029.123746
GTACATG86900.027.4018961
TACATGG89000.026.2323842
ACATGGG91700.025.0977673
ACGCAGA75350.024.2027287
CGCAGAG77150.023.3302638
TAGATCG1652.4316087E-623.0306955
CATGGGG73650.022.9379864
TAAGGTG13400.019.49665
ATGGGGG41850.019.1819275
GCAGAGT90400.018.5976859
AGAGTAC73700.018.26878410-11
GTAAGGT11800.017.2927674
CGTACAC4801.8189894E-1216.806233
Produced by FastQC (version 0.11.5)