##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921675_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2826058
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.299519330459603	33.0	31.0	34.0	28.0	34.0
2	31.431450805326712	33.0	31.0	34.0	28.0	34.0
3	31.500827300784344	34.0	31.0	34.0	28.0	34.0
4	34.98718816103562	37.0	35.0	37.0	32.0	37.0
5	34.73081691883181	37.0	35.0	37.0	32.0	37.0
6	34.704668835529915	37.0	35.0	37.0	32.0	37.0
7	34.586688595916996	37.0	35.0	37.0	32.0	37.0
8	34.530259110039495	37.0	35.0	37.0	31.0	37.0
9	36.10582337659029	39.0	37.0	39.0	32.0	39.0
10-11	35.99635304724815	39.0	37.0	39.0	31.0	39.0
12-13	35.92015025877035	39.0	37.0	39.0	30.5	39.0
14-15	37.140191036418926	40.0	38.0	41.0	31.0	41.0
16-17	37.10781236620055	40.0	38.0	41.0	31.0	41.0
18-19	37.07360995421892	40.0	38.0	41.0	31.0	41.0
20-21	37.01696532767551	40.0	37.5	41.0	31.0	41.0
22-23	36.92830791158568	40.0	37.0	41.0	30.0	41.0
24-25	36.842594879510614	40.0	37.0	41.0	30.0	41.0
26-27	36.71281233435407	40.0	37.0	41.0	30.0	41.0
28-29	36.58966217253857	40.0	37.0	41.0	30.0	41.0
30-31	36.43427151884356	40.0	36.5	41.0	30.0	41.0
32-33	36.37304648383012	40.0	36.5	41.0	29.5	41.0
34-35	36.49345413293004	40.0	37.0	41.0	30.0	41.0
36-37	36.48401943626068	40.0	37.0	41.0	30.0	41.0
38-39	36.37558588677231	40.0	37.0	41.0	29.5	41.0
40-41	36.29447467107894	40.0	36.5	41.0	29.0	41.0
42-43	36.16947759033962	40.0	36.0	41.0	28.5	41.0
44-45	36.03225464587068	40.0	36.0	41.0	28.0	41.0
46-47	35.86928895302219	40.0	36.0	41.0	27.5	41.0
48-49	35.72318473293896	40.0	35.0	41.0	27.0	41.0
50-51	35.56337396472401	39.5	35.0	41.0	27.0	41.0
52-53	35.391131569132696	39.0	35.0	41.0	26.0	41.0
54-55	35.17931974503	39.0	35.0	41.0	26.0	41.0
56-57	34.953002026143835	39.0	35.0	41.0	26.0	41.0
58-59	34.7090073876757	38.5	35.0	40.5	25.0	41.0
60-61	34.432827988668315	38.0	34.0	40.0	24.5	41.0
62-63	34.13594837756338	37.5	34.0	40.0	23.5	41.0
64-65	33.80202034070072	37.0	34.0	40.0	23.0	41.0
66-67	33.454987654181195	36.5	34.0	39.0	22.0	41.0
68-69	33.08798067838664	36.0	33.0	39.0	22.0	41.0
70-71	32.68951061867803	35.5	33.0	39.0	20.5	40.0
72-73	32.32554285863914	35.0	33.0	37.5	20.0	40.0
74-75	31.923398599745653	35.0	32.5	37.0	18.5	39.0
76-77	29.212703348622	33.0	29.0	34.5	11.0	36.5
78-79	30.769598677734145	34.5	31.0	36.0	12.0	37.5
80-81	30.94611593251094	35.0	32.0	36.0	13.0	37.0
82-83	30.758293177280862	35.0	32.0	35.0	10.5	37.0
84-85	30.54959240043906	35.0	32.0	35.0	7.0	36.5
86-87	30.332764578787838	35.0	32.0	35.0	4.5	36.0
88-89	30.04837038022574	35.0	31.0	35.0	2.0	36.0
90-91	29.841807917601123	34.0	31.0	35.0	2.0	35.0
92-93	29.671406425487376	34.0	31.0	35.0	2.0	35.0
94-95	29.556090320863902	34.0	31.0	35.0	2.0	35.0
96-97	29.342132928623545	34.0	31.0	35.0	2.0	35.0
98-99	29.124378020550182	34.0	31.0	35.0	2.0	35.0
100-101	27.99838078340926	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	55670.0
3	18438.0
4	10025.0
5	7093.0
6	6232.0
7	6253.0
8	6463.0
9	7148.0
10	7911.0
11	8633.0
12	9341.0
13	9900.0
14	10691.0
15	11935.0
16	12576.0
17	13644.0
18	12438.0
19	11323.0
20	11024.0
21	10913.0
22	11528.0
23	12860.0
24	15344.0
25	18360.0
26	21927.0
27	26089.0
28	31421.0
29	38219.0
30	45836.0
31	57387.0
32	72089.0
33	94429.0
34	133568.0
35	198080.0
36	342823.0
37	659459.0
38	706351.0
39	92636.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.077590026620726	19.712382714605244	13.419327853220894	25.790699405553134
2	15.136465802517716	21.033128855196075	38.060816912332946	25.769588429953266
3	17.75384154785156	24.882146291124936	29.54179582121413	27.822216339809376
4	12.875327196529533	16.957565038548765	35.87379741894709	34.29331034597461
5	13.726791436695809	37.79443171449424	34.60382719641192	13.874949652398033
6	33.177246992215146	36.13191790516631	16.947912243453644	13.742922859164896
7	28.566398849563594	32.18200051095908	21.063368126202647	18.188232513274674
8	26.395742762533537	35.37783017899845	19.83618170610794	18.390245352360072
9	26.74541711458151	15.229906817199081	19.37079847618131	38.6538775920381
10-11	25.460970924795394	25.458899801720946	28.185754603071032	20.894374670412628
12-13	25.929008358306344	23.031926883131266	27.820251651509476	23.218813107052917
14-15	23.19536418275249	24.5912333710616	25.0642315332462	27.149170912939713
16-17	23.349450011287807	27.66512576882711	25.90352002683597	23.081904193049116
18-19	23.255528811959174	27.03275709179077	27.188081870790448	22.523632225459604
20-21	23.9112146085146	26.169224506073054	27.07464003208718	22.844920853325164
22-23	23.782141729246487	26.002567180604075	26.753808087768917	23.461483002380525
24-25	23.50402187651714	26.18825113834038	26.770159983354823	23.537567001787657
26-27	23.29073163050373	26.431904656168253	26.767274744270352	23.510088969057662
28-29	23.40572687933963	26.671442693254356	26.61156837624002	23.311262051166
30-31	23.818052995620032	26.31453868782188	26.398379525423344	23.469028791134743
32-33	23.590738837598416	26.381179982843406	26.28076366652794	23.747317513030232
34-35	23.87255269725285	26.455163983970355	26.42104931622032	23.251234002556476
36-37	23.93527416704574	26.20886317718587	26.433743266203706	23.42211938956468
38-39	23.73465508044334	26.321863585578143	26.47914984036949	23.46433149360903
40-41	23.921329597739536	26.155590961325636	26.487388832320256	23.43569060861457
42-43	23.77362670378652	26.11728035391021	26.247721221748943	23.861371720554327
44-45	23.63660555784689	26.17116096141335	26.431064095343704	23.76116938539606
46-47	23.754836182420615	26.157414984305355	26.237794649506263	23.849954183767768
48-49	23.513996129261024	26.283177573070017	26.266492133071605	23.936334164597355
50-51	23.66714803206948	26.327278697829325	26.276022305819307	23.729550964281884
52-53	23.891615941367427	26.118610505427093	26.271564886981842	23.71820866622364
54-55	23.30395020562174	26.070178735122436	26.542976915834952	24.082894143420873
56-57	23.46278179723869	26.236404460982328	26.30022630479133	24.00058743698765
58-59	23.484335724845984	26.045828920581076	26.457190330963055	24.01264502360988
60-61	23.502143516995705	26.17574946126762	26.513241253550042	23.808865768186628
62-63	23.445789093864065	26.138593782129988	26.490521643200683	23.92509548080527
64-65	23.18574879500671	25.98879186325096	26.466116466636834	24.35934287510549
66-67	23.213740076923866	26.153300351124916	26.24953484889095	24.38342472306027
68-69	23.374495066610493	26.037728835281083	26.311145176536392	24.27663092157203
70-71	23.259995956258187	25.901299415002992	26.282728371381214	24.55597625735761
72-73	23.237044450756922	26.0828096779132	26.155161291178963	24.524984580150917
74-75	23.1668812465988	26.17627409599908	26.135304814111837	24.521539843290284
76-77	23.155427830650016	25.86709535889822	26.26707017648086	24.710406633970905
78-79	23.119257054628825	25.91380222938153	26.297383920098277	24.669556795891367
80-81	23.321369280611243	26.104786405984516	26.016069588260844	24.557774725143403
82-83	23.207939617902614	26.093446849146872	26.095092706921047	24.60352082602947
84-85	23.203314862946637	26.096208275014103	26.022835352755592	24.677641509283667
86-87	23.153879697328563	26.17956718175955	25.976105464413056	24.690447656498833
88-89	23.009129392925892	25.940993969575317	26.0573117479714	24.992564889527387
90-91	22.960289526183743	25.904582993353493	26.093415990877244	25.041711489585527
92-93	23.02105675346314	25.777564010420388	26.210336694209374	24.991042541907095
94-95	22.84821083534929	25.735936312278202	26.198515246012793	25.217337606359713
96-97	22.79280793738708	25.61654719415065	26.257752737014773	25.332892131447487
98-99	22.642699514107136	25.58305639934686	26.13174042992044	25.642503656625564
100-101	22.563744812332818	25.635254251202248	26.167675169631433	25.633325766833504
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	39.5
2	58.5
3	86.5
4	77.0
5	85.0
6	141.0
7	305.5
8	466.0
9	479.5
10	404.0
11	339.5
12	350.0
13	386.5
14	411.0
15	441.0
16	489.5
17	551.5
18	637.0
19	739.5
20	815.5
21	948.0
22	1140.0
23	1352.5
24	1648.0
25	2119.0
26	2889.5
27	4076.5
28	5963.5
29	8672.5
30	12644.5
31	18346.5
32	26407.0
33	32964.0
34	37821.5
35	44721.5
36	53426.5
37	62169.5
38	69756.5
39	77157.5
40	83211.0
41	88098.0
42	93467.5
43	99792.0
44	105789.0
45	112252.0
46	117682.5
47	121559.5
48	124669.0
49	126370.5
50	125904.5
51	123856.5
52	121178.5
53	118684.5
54	115302.0
55	108820.0
56	99441.0
57	88050.0
58	78845.0
59	68636.0
60	55950.0
61	45062.0
62	36496.5
63	27820.5
64	19459.5
65	13689.0
66	9816.0
67	7168.0
68	5212.0
69	3766.5
70	2736.5
71	1888.5
72	1296.5
73	898.0
74	596.5
75	381.0
76	236.0
77	138.5
78	82.5
79	47.5
80	39.5
81	30.5
82	19.5
83	15.5
84	8.0
85	7.0
86	9.0
87	5.0
88	5.0
89	6.0
90	3.0
91	1.5
92	1.5
93	2.5
94	2.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17508487086959998
2	7.076995588908649E-5
3	0.010933958184863864
4	0.00555544153729329
5	0.02476948456118027
6	0.0020523287207835084
7	0.0
8	0.0
9	0.0
10-11	0.05320131433962077
12-13	2.476948456118027E-4
14-15	0.005254669224764672
16-17	0.0
18-19	0.0015569390295599027
20-21	2.653873345840744E-4
22-23	1.769248897227162E-5
24-25	4.953896912236054E-4
26-27	0.0026715658348130155
28-29	0.00490081944531924
30-31	0.016489399722157155
32-33	0.011393962898142925
34-35	0.010403183515695715
36-37	0.003803885129038399
38-39	1.769248897227162E-5
40-41	0.0032023405039811634
42-43	0.009076246842775342
44-45	0.006988533144047291
46-47	0.0033438804157593367
48-49	0.008527779684634923
50-51	0.002441563478173484
52-53	0.001840018853116249
54-55	0.004122349930539289
56-57	0.007890850081633144
58-59	0.015587082784571299
60-61	0.007483922835270896
62-63	0.021974071303561354
64-65	0.04055118472444656
66-67	0.06040215735133532
68-69	0.05052974850480776
70-71	0.06868224219035844
72-73	0.0341641962054565
74-75	0.02726412550627057
76-77	0.234620096261294
78-79	0.08986015149016757
80-81	0.06735530551743807
82-83	0.027724130219549635
84-85	0.051184370596781804
86-87	0.06862916472344162
88-89	0.2953230259251579
90-91	0.30038307777122764
92-93	0.056474424799491024
94-95	0.009553944045026677
96-97	0.01567554522943266
98-99	8.315469816967663E-4
100-101	1.592324007504446E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2826058.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.058802046738315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.06805964487825	46.886741403847026
2	12.440510264925907	14.943242867232021
3	3.969417468367084	7.151953739205716
4	1.7510397546417737	4.2066139999999805
5	0.9811245527952724	2.946258264976296
6	0.5811025544322006	2.094019397129851
7	0.4116351486517024	1.7305619735846611
8	0.3026166186301099	1.453983327548726
9	0.21983377420113667	1.1882657815140094
>10	1.2045703415687656	12.838847974651033
>50	0.05404382478665877	2.2030940491193522
>100	0.014449406347525926	1.4654681183055933
>500	9.461604585384138E-4	0.4024294142329723
>1k	6.504853151399348E-4	0.4885196886527273
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.538497794454325E-5	0.0
5	0.0	0.0	0.0	3.538497794454325E-5	0.0
6	0.0	0.0	0.0	3.538497794454325E-5	3.538497794454325E-5
7	0.0	0.0	0.0	3.538497794454325E-5	3.538497794454325E-5
8	0.0	0.0	0.0	3.538497794454325E-5	3.538497794454325E-5
9	0.0	0.0	0.0	7.07699558890865E-5	3.538497794454325E-5
10-11	0.0	0.0	0.0	7.07699558890865E-5	3.538497794454325E-5
12-13	0.0	0.0	0.0	1.2384742280590136E-4	3.538497794454325E-5
14-15	0.0	0.0	0.0	1.41539911778173E-4	8.846244486135813E-5
16-17	0.0	0.0	0.0	1.41539911778173E-4	1.0615493383362974E-4
18-19	0.0	0.0	0.0	1.7692488972271625E-4	1.41539911778173E-4
20-21	0.0	0.0	0.0	2.476948456118027E-4	1.41539911778173E-4
22-23	0.0	0.0	0.0	3.7154226841770407E-4	1.9461737869498787E-4
24-25	0.0	0.0	0.0	6.192371140295069E-4	2.83079823556346E-4
26-27	0.0	0.0	0.0	0.001079241827308569	3.184648015008892E-4
28-29	0.0	0.0	0.0	0.0036977301952047697	3.3615729047316084E-4
30-31	0.0	3.538497794454325E-5	0.0	0.01622401238757308	3.5384977944543245E-4
32-33	0.0	3.538497794454325E-5	0.0	0.04635432110735166	3.5384977944543245E-4
34-35	0.0	3.538497794454325E-5	0.0	0.08641011614057462	3.5384977944543245E-4
36-37	0.0	3.538497794454325E-5	0.0	0.1515007830695619	3.5384977944543245E-4
38-39	0.0	3.538497794454325E-5	0.0	0.25753186948038576	3.5384977944543245E-4
40-41	0.0	3.538497794454325E-5	0.0	0.3789377288081136	3.5384977944543245E-4
42-43	0.0	3.538497794454325E-5	0.0	0.5035105436618781	3.7154226841770407E-4
44-45	0.0	3.538497794454325E-5	0.0	0.6591867541288962	3.8923475738997574E-4
46-47	0.0	3.538497794454325E-5	0.0	0.841295543120488	4.0692724636224735E-4
48-49	0.0	3.538497794454325E-5	0.0	1.0293667008957352	4.600047132790622E-4
50-51	0.0	3.538497794454325E-5	0.0	1.2267264153814252	4.953896912236054E-4
52-53	0.0	3.538497794454325E-5	0.0	1.4244045946686161	4.953896912236054E-4
54-55	0.0	3.538497794454325E-5	0.0	1.653345401969811	4.953896912236054E-4
56-57	0.0	3.538497794454325E-5	0.0	1.9205373704290571	4.953896912236054E-4
58-59	0.0	3.538497794454325E-5	0.0	2.1961332711501322	4.953896912236054E-4
60-61	0.0	3.538497794454325E-5	0.0	2.4874931795455013	5.130821801958771E-4
62-63	0.0	3.538497794454325E-5	0.0	2.7827277430257977	6.015446250572352E-4
64-65	0.0	3.538497794454325E-5	0.0	3.108411080027374	6.015446250572352E-4
66-67	0.0	3.538497794454325E-5	0.0	3.4862872594971512	6.015446250572352E-4
68-69	0.0	3.538497794454325E-5	0.0	3.8988053323746366	6.192371140295069E-4
70-71	0.0	3.538497794454325E-5	0.0	4.3292989740479495	6.369296030017784E-4
72-73	0.0	3.538497794454325E-5	0.0	4.770142721770041	7.253920478631366E-4
74-75	0.0	3.538497794454325E-5	0.0	5.2349774845385335	7.430845368354082E-4
76-77	0.0	3.538497794454325E-5	0.0	5.754181265918817	7.784695147799515E-4
78-79	0.0	3.538497794454325E-5	0.0	6.325170962520939	8.138544927244947E-4
80-81	0.0	3.538497794454325E-5	0.0	6.917108566066231	8.492394706690379E-4
82-83	0.0	3.538497794454325E-5	0.0	7.534788741066178	9.200094265581244E-4
84-85	0.0	3.538497794454325E-5	0.0	8.175858386487468	9.200094265581244E-4
86-87	0.0	3.538497794454325E-5	0.0	8.864184669953696	9.377019155303961E-4
88-89	0.0	3.538497794454325E-5	0.0	9.603960711351288	9.553944045026677E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2605	0.0	51.452206	1
GTATCAA	4605	0.0	42.007557	1
TCAACGC	5715	0.0	32.549767	4
TATCAAC	5985	0.0	31.318115	2
CAACGCA	5955	0.0	31.268373	5
ATCAACG	6070	0.0	30.80138	3
AACGCAG	6425	0.0	29.12374	6
GTACATG	8690	0.0	27.401896	1
TACATGG	8900	0.0	26.232384	2
ACATGGG	9170	0.0	25.097767	3
ACGCAGA	7535	0.0	24.202728	7
CGCAGAG	7715	0.0	23.330263	8
TAGATCG	165	2.4316087E-6	23.030695	5
CATGGGG	7365	0.0	22.937986	4
TAAGGTG	1340	0.0	19.4966	5
ATGGGGG	4185	0.0	19.181927	5
GCAGAGT	9040	0.0	18.597685	9
AGAGTAC	7370	0.0	18.268784	10-11
GTAAGGT	1180	0.0	17.292767	4
CGTACAC	480	1.8189894E-12	16.80623	3
>>END_MODULE