FastQCFastQC Report
Mon 15 Aug 2016
SRR2921674_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921674_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2275842
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.047.9043051
GTATCAA31800.039.4013751
TCAACGC34800.034.777274
CAACGCA36300.033.2065285
ATCAACG38150.031.7241333
AACGCAG38350.031.5371886
GTACATG53000.030.715161
TATCAAC40650.029.6569732
TACATGG58250.027.5406652
ACATGGG57200.026.9669273
ACGCAGA46300.025.8187087
CGCAGAG48150.024.2371988
GAGTACA38500.022.435991
CATGGGG43750.022.347234
AGTACAT42250.019.0974712
GCAGAGT56150.019.0110029
TATGTCG2051.6030895E-518.5196385
AGAGTAC46150.018.50472310-11
TAAGGTG11400.017.9002865
ATGGGGG25500.017.8660035
Produced by FastQC (version 0.11.5)