##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921674_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2275842
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.94904347489852	31.0	31.0	34.0	26.0	34.0
2	29.99534106497727	31.0	31.0	34.0	25.0	34.0
3	30.05388027815639	31.0	31.0	34.0	25.0	34.0
4	33.49878550444187	37.0	35.0	37.0	28.0	37.0
5	33.49365158038212	37.0	35.0	37.0	28.0	37.0
6	33.53649154906184	37.0	35.0	37.0	28.0	37.0
7	33.43865742876702	37.0	35.0	37.0	27.0	37.0
8	33.40391951638119	37.0	35.0	37.0	27.0	37.0
9	34.90721675757808	39.0	35.0	39.0	27.0	39.0
10-11	34.795360793939125	39.0	35.0	39.0	27.0	39.0
12-13	34.67763667249308	39.0	35.0	39.0	27.0	39.0
14-15	35.76214956925833	40.0	36.0	41.0	27.0	41.0
16-17	35.6951585391253	40.0	36.0	41.0	27.0	41.0
18-19	35.666403247677124	40.0	36.0	41.0	26.5	41.0
20-21	35.586134494398124	40.0	36.0	41.0	26.0	41.0
22-23	35.48604230873672	40.0	36.0	41.0	25.0	41.0
24-25	35.37488894220249	40.0	36.0	41.0	25.0	41.0
26-27	35.22262639497821	39.5	35.0	41.0	24.0	41.0
28-29	35.07695657255644	39.0	35.0	41.0	24.0	41.0
30-31	34.929494666149935	39.0	35.0	41.0	23.5	41.0
32-33	34.74469646838401	39.0	35.0	41.0	22.5	41.0
34-35	34.57075469210956	39.0	34.0	41.0	21.5	41.0
36-37	34.532257511725334	39.0	34.5	41.0	21.0	41.0
38-39	34.708556437573435	39.0	35.0	41.0	21.5	41.0
40-41	34.76613007405611	39.5	35.0	41.0	21.5	41.0
42-43	34.6028408386874	39.0	35.0	41.0	18.5	41.0
44-45	34.47815775436081	39.0	35.0	41.0	17.0	41.0
46-47	34.318239798720654	39.0	34.0	41.0	15.0	41.0
48-49	34.14618611485332	39.0	34.0	41.0	13.5	41.0
50-51	32.48539463635876	37.0	32.0	39.5	10.5	40.0
52-53	32.602355084403925	37.5	32.0	39.5	8.0	40.0
54-55	33.5081930116414	38.0	33.0	40.0	8.0	41.0
56-57	33.53885397140927	38.0	33.0	41.0	7.5	41.0
58-59	33.35800661908867	38.0	33.0	40.0	4.0	41.0
60-61	33.11398462634928	38.0	33.0	40.0	2.0	41.0
62-63	32.82980936286438	37.0	33.0	40.0	2.0	41.0
64-65	32.550804493457804	37.0	32.5	40.0	2.0	41.0
66-67	32.21620437622647	36.0	32.0	39.5	2.0	41.0
68-69	31.85769926031772	36.0	32.0	39.0	2.0	41.0
70-71	31.451073492799587	35.0	32.0	39.0	2.0	40.5
72-73	31.097483920236993	35.0	31.0	37.5	2.0	40.0
74-75	30.70209794880312	35.0	31.0	37.0	2.0	39.0
76-77	30.325891472255105	35.0	31.0	36.5	2.0	39.0
78-79	29.97756544610742	35.0	31.0	36.0	2.0	38.5
80-81	29.661174853087342	35.0	30.0	36.0	2.0	37.0
82-83	29.34311059379342	35.0	30.0	35.0	2.0	37.0
84-85	29.076380302323273	35.0	30.0	35.0	2.0	36.5
86-87	28.84052407856081	34.0	30.0	35.0	2.0	36.0
88-89	28.616293222464478	34.0	29.5	35.0	2.0	36.0
90-91	28.381220225305622	34.0	29.0	35.0	2.0	35.5
92-93	28.181750095129626	34.0	29.0	35.0	2.0	35.0
94-95	27.946541324046223	34.0	29.0	35.0	2.0	35.0
96-97	27.705969702641923	34.0	29.0	35.0	2.0	35.0
98-99	27.41768826658441	34.0	29.0	35.0	2.0	35.0
100-101	26.34901544131798	33.0	25.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	95850.0
3	15821.0
4	10534.0
5	7962.0
6	6931.0
7	7317.0
8	7860.0
9	8194.0
10	8915.0
11	9660.0
12	9428.0
13	9675.0
14	10027.0
15	10653.0
16	11099.0
17	11809.0
18	11266.0
19	10259.0
20	10509.0
21	11040.0
22	12379.0
23	13589.0
24	16016.0
25	18991.0
26	22474.0
27	26499.0
28	30711.0
29	35947.0
30	43166.0
31	52090.0
32	64010.0
33	82653.0
34	112531.0
35	161466.0
36	264083.0
37	462086.0
38	483329.0
39	88996.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.92249443970859	17.846963722588168	11.95870414765426	25.271837690048983
2	16.02721588593003	19.09513731287759	39.380804735736525	25.496842065455848
3	19.639715539905627	23.1540456151012	29.008272348598606	28.19796649639456
4	13.004515853856944	14.937450807093452	36.75905039552197	35.29898294352764
5	13.837182051499644	37.35340039845104	34.82722429293846	13.982193257110847
6	34.25479285067997	35.374746938099314	16.50563426639404	13.864825944826684
7	29.492506958671598	31.507511498177603	20.64332666250069	18.356654880650105
8	26.922923107686152	35.10748399770595	19.50932890345008	18.460263991157813
9	26.623573793615606	15.428098105861702	19.19998418072641	38.748343919796284
10-11	25.804520873378106	25.14068953030834	27.797904532386937	21.256885063926614
12-13	26.10122398141273	22.57831369251057	27.682935217258404	23.637527108818297
14-15	23.06382221167255	24.320200092089834	24.68805392640066	27.927923769836955
16-17	23.40613962008818	27.643717350950244	25.679417784113625	23.27072524484796
18-19	23.477326583546738	26.719047823072977	27.07736657251681	22.726259020863477
20-21	23.614912933177905	26.107015996035877	27.18416371573004	23.09390735505618
22-23	23.51946869707443	26.01589618687558	26.818397801153488	23.6462373148965
24-25	23.37602713579082	26.3490919975658	26.87037117213421	23.404509694509173
26-27	23.18862012612808	26.4512101356012	26.868106525245945	23.49206321302478
28-29	23.4163433802604	26.39643028527132	26.838272130104396	23.348954204363885
30-31	23.550401819310398	26.273676578026556	26.64369091498349	23.532230687679558
32-33	23.2543357211393	26.416825540024767	26.604045991942115	23.724792746893815
34-35	23.745270196934694	26.434803968310817	26.628368316262613	23.191557518491877
36-37	23.845124635847142	26.12354613481675	26.599438446632657	23.431890782703455
38-39	23.498312869080486	26.44561787572223	26.64170647393373	23.414362781263552
40-41	23.641901301926623	26.258211602457926	26.82851166360074	23.27137543201471
42-43	23.501325255251473	26.142366503325693	26.641286127409487	23.71502211401335
44-45	23.450871164823013	26.269894664170497	26.76493871994336	23.514295451063134
46-47	23.761360011219384	26.294197513550333	26.43725786463162	23.507184610598664
48-49	23.44650761865025	26.519243958637684	26.33535271692044	23.698895705791628
50-51	23.51808921207958	26.502341371270816	26.473302643426877	23.506266773222727
52-53	23.787170894743564	26.234340116163864	26.520742603250653	23.457746385841922
54-55	23.228535937863963	26.289873655723405	26.851868568134897	23.62972183827774
56-57	23.32350417630923	26.466948294436133	26.636039444914267	23.573508084340375
58-59	23.360608540770897	26.17072337760738	26.806308154135415	23.662359927486307
60-61	23.25972948405372	26.44024285835083	26.844470947193372	23.455556710402075
62-63	23.338961552780905	26.26214967746538	26.87161781182842	23.527270957925296
64-65	23.074568971306146	26.133783415745942	26.893847907400797	23.897799705547115
66-67	23.081314477419447	26.29232243982621	26.74110223170194	23.8852608510524
68-69	23.345130452241484	26.073320605712635	26.90198822108018	23.679560720965704
70-71	23.216801504643897	25.98933770309347	26.791035182321842	24.002825609940793
72-73	23.189640273831763	26.2038591388984	26.69822255930765	23.90827802796219
74-75	23.083292384157865	26.350442163032316	26.737654043753096	23.828611409056723
76-77	23.185812576417135	25.968574147022938	26.92384064993257	23.92177262662735
78-79	23.124410451619383	25.954110566908845	26.863963720370656	24.057515261101127
80-81	23.239415064079623	26.177725583426394	26.669341468135695	23.91351788435829
82-83	23.24670096904286	26.1656106273653	26.782960911938787	23.804727491653054
84-85	23.10167973301837	26.14816106623544	26.799739113355336	23.95042008739086
86-87	23.072906297185728	26.232555045976625	26.764999445003358	23.929539211834292
88-89	23.001155061183006	26.03875676217301	26.72738904420614	24.23269913243784
90-91	23.051732818385197	26.042248005846027	26.847992367397406	24.058026808371373
92-93	23.205500587898346	25.802934360417474	27.054753718052364	23.936811333631816
94-95	23.023722814213425	25.56404340564083	27.152178739058737	24.260055041087007
96-97	23.113845440913984	25.304143165996223	27.201332247941544	24.380679145148246
98-99	22.86212141934202	25.373765777337017	27.182354973812995	24.581757829507968
100-101	23.049593764708394	25.222576502379003	27.282021712427834	24.44580802048477
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	21.0
2	35.5
3	55.0
4	50.5
5	58.0
6	112.5
7	231.5
8	367.0
9	439.0
10	425.5
11	396.0
12	420.0
13	504.0
14	594.0
15	661.5
16	748.0
17	793.0
18	875.0
19	963.0
20	1033.5
21	1198.5
22	1387.0
23	1593.0
24	1907.0
25	2378.5
26	3165.5
27	4344.5
28	5878.0
29	8154.0
30	11479.5
31	16375.5
32	24106.5
33	29905.0
34	34163.5
35	40645.5
36	49402.0
37	57622.0
38	62910.0
39	68465.5
40	71605.5
41	73438.0
42	76503.5
43	79550.0
44	83105.0
45	88037.5
46	90348.5
47	90587.5
48	92177.0
49	92747.0
50	92109.5
51	90786.0
52	89638.5
53	88954.0
54	87608.5
55	83313.0
56	76709.0
57	70089.0
58	63749.0
59	55597.0
60	45759.0
61	37739.0
62	31418.5
63	23921.5
64	16938.5
65	12437.0
66	9276.0
67	7161.5
68	5400.0
69	3950.0
70	2941.0
71	2153.0
72	1581.5
73	1200.0
74	902.5
75	654.5
76	472.0
77	330.0
78	226.0
79	154.0
80	119.5
81	99.5
82	73.5
83	60.5
84	53.0
85	44.5
86	34.5
87	32.5
88	27.0
89	17.0
90	24.5
91	25.0
92	18.5
93	17.0
94	13.5
95	12.0
96	9.0
97	4.5
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.054089870913710175
2	0.07597188205508115
3	0.07201730172832736
4	0.07170972325846874
5	0.06744756446185632
6	0.009359173439983971
7	0.01160010229181112
8	0.0170046954050413
9	0.006019750052947436
10-11	0.01160010229181112
12-13	0.022914596004467797
14-15	0.03908443556274996
16-17	0.026517658079954584
18-19	0.009425083112096534
20-21	0.019091835021939133
22-23	0.03651395835036
24-25	0.03363590266811141
26-27	0.020058510212923392
28-29	0.011160704477727363
30-31	0.011182674368431551
32-33	0.00801901010702852
34-35	0.004965195299146426
36-37	5.272773769005054E-4
38-39	0.003734881419711913
40-41	9.886450816884475E-4
42-43	0.23446267359509143
44-45	0.20344118792077834
46-47	0.20970260677147184
48-49	0.21682085135962867
50-51	0.2081427445314745
52-53	0.21583220627794023
54-55	0.21200944529541155
56-57	0.21431628381935125
58-59	0.22402697551060224
60-61	0.22927777938890312
62-63	0.21291021081428324
64-65	0.21312990972132512
66-67	0.2106912518531603
68-69	0.20502302005147985
70-71	0.19658658202107177
72-73	0.20825259398499543
74-75	0.2080109251872494
76-77	0.2017275364458517
78-79	0.22128073917257873
80-81	0.23149673835002604
82-83	0.23072779217537948
84-85	0.2251474399365158
86-87	0.24421730506775075
88-89	0.23725284971452326
90-91	0.24522792004014338
92-93	0.24019681506888438
94-95	0.24333850943958324
96-97	0.2501931153392898
98-99	0.24327259976747068
100-101	0.24902871113196787
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2275842.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.8595096819159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.1525919630912	47.978948546453125
2	11.248149910231755	13.466174769103183
3	3.5429936603241154	6.36245589939414
4	1.611047162053837	3.857459727799391
5	0.8605234250155067	2.5755255145615576
6	0.5601236915686247	2.011723772311354
7	0.3727252836823021	1.5617806909092928
8	0.2569525455680713	1.230484271137992
9	0.19489390764956105	1.049962837670579
>10	1.0888433386194258	11.75121634506257
>50	0.07459080518796161	3.0702433555173467
>100	0.033986899476362104	3.6489780017635933
>500	0.0016200847339244778	0.6555677500352662
>1k	9.573227973186309E-4	0.7794785182806656
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.393978140837545E-5
2	0.0	0.0	0.0	0.0	4.393978140837545E-5
3	0.0	0.0	0.0	0.0	4.393978140837545E-5
4	0.0	0.0	0.0	0.0	4.393978140837545E-5
5	0.0	0.0	0.0	0.0	4.393978140837545E-5
6	0.0	0.0	0.0	0.0	4.393978140837545E-5
7	0.0	0.0	0.0	0.0	4.393978140837545E-5
8	0.0	0.0	0.0	0.0	4.393978140837545E-5
9	0.0	0.0	0.0	0.0	8.78795628167509E-5
10-11	0.0	0.0	0.0	4.393978140837545E-5	8.78795628167509E-5
12-13	0.0	0.0	0.0	4.393978140837545E-5	1.757591256335018E-4
14-15	2.1969890704187726E-5	0.0	0.0	4.393978140837545E-5	1.757591256335018E-4
16-17	4.393978140837545E-5	0.0	0.0	4.393978140837545E-5	1.757591256335018E-4
18-19	6.590967211256318E-5	0.0	0.0	4.393978140837545E-5	1.757591256335018E-4
20-21	8.78795628167509E-5	0.0	0.0	6.590967211256318E-5	1.757591256335018E-4
22-23	8.78795628167509E-5	0.0	0.0	8.78795628167509E-5	2.1969890704187726E-4
24-25	8.78795628167509E-5	0.0	0.0	1.3181934422512636E-4	3.0757846985862816E-4
26-27	8.78795628167509E-5	0.0	0.0	3.7348814197119137E-4	3.0757846985862816E-4
28-29	8.78795628167509E-5	0.0	0.0	0.0025924471030941516	3.0757846985862816E-4
30-31	8.78795628167509E-5	0.0	0.0	0.012325108685049314	3.2954836056281586E-4
32-33	1.3181934422512636E-4	0.0	0.0	0.03339423387036534	3.9545803267537907E-4
34-35	1.3181934422512636E-4	0.0	0.0	0.06160357353454238	4.8333759549212997E-4
36-37	1.3181934422512636E-4	0.0	0.0	0.10604866242911415	4.8333759549212997E-4
38-39	1.3181934422512636E-4	0.0	0.0	0.18019704355574773	5.272773769005054E-4
40-41	1.3181934422512636E-4	0.0	0.0	0.2668243226023599	5.272773769005054E-4
42-43	1.3181934422512636E-4	0.0	0.0	0.3548137348726318	5.272773769005054E-4
44-45	1.3181934422512636E-4	0.0	0.0	0.4603351199248454	5.272773769005054E-4
46-47	1.3181934422512636E-4	0.0	0.0	0.5867938108181499	5.712171583088808E-4
48-49	1.3181934422512636E-4	0.0	0.0	0.7274889908877682	6.151569397172563E-4
50-51	1.3181934422512636E-4	0.0	0.0	0.8790153270745509	7.030365025340072E-4
52-53	1.3181934422512636E-4	0.0	0.0	1.0299045364309123	8.348558467591335E-4
54-55	1.3181934422512636E-4	0.0	0.0	1.2005007377489298	8.78795628167509E-4
56-57	1.3181934422512636E-4	0.0	0.0	1.396560042393101	8.78795628167509E-4
58-59	1.3181934422512636E-4	0.0	0.0	1.6079982705302038	8.78795628167509E-4
60-61	1.3181934422512636E-4	0.0	0.0	1.840923051776002	8.78795628167509E-4
62-63	1.3181934422512636E-4	0.0	0.0	2.0841517117620643	8.78795628167509E-4
64-65	1.5378923492931408E-4	0.0	0.0	2.3408039749683853	9.227354095758844E-4
66-67	1.757591256335018E-4	0.0	0.0	2.631970936470985	9.227354095758844E-4
68-69	1.757591256335018E-4	0.0	0.0	2.9458987047431235	9.227354095758844E-4
70-71	1.757591256335018E-4	0.0	0.0	3.290122952296337	9.227354095758844E-4
72-73	1.757591256335018E-4	0.0	0.0	3.6510662866754373	0.0011424343166177616
74-75	1.757591256335018E-4	0.0	0.0	4.011636132912566	0.0011424343166177616
76-77	1.757591256335018E-4	0.0	0.0	4.411641932963711	0.0011424343166177616
78-79	2.1969890704187726E-4	0.0	0.0	4.866792158682369	0.0011424343166177616
80-81	2.1969890704187726E-4	0.0	0.0	5.356259353680967	0.0012083439887303248
82-83	2.1969890704187726E-4	0.0	0.0	5.858249386380953	0.0013181934422512634
84-85	2.1969890704187726E-4	0.0	0.0	6.368412218422896	0.0013181934422512634
86-87	2.1969890704187726E-4	0.0	0.0	6.905114678435497	0.001340163332955451
88-89	2.1969890704187726E-4	0.0	0.0	7.494456996575334	0.001362133223659639
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1605	0.0	47.904305	1
GTATCAA	3180	0.0	39.401375	1
TCAACGC	3480	0.0	34.77727	4
CAACGCA	3630	0.0	33.206528	5
ATCAACG	3815	0.0	31.724133	3
AACGCAG	3835	0.0	31.537188	6
GTACATG	5300	0.0	30.71516	1
TATCAAC	4065	0.0	29.656973	2
TACATGG	5825	0.0	27.540665	2
ACATGGG	5720	0.0	26.966927	3
ACGCAGA	4630	0.0	25.818708	7
CGCAGAG	4815	0.0	24.237198	8
GAGTACA	3850	0.0	22.43599	1
CATGGGG	4375	0.0	22.34723	4
AGTACAT	4225	0.0	19.097471	2
GCAGAGT	5615	0.0	19.011002	9
TATGTCG	205	1.6030895E-5	18.519638	5
AGAGTAC	4615	0.0	18.504723	10-11
TAAGGTG	1140	0.0	17.900286	5
ATGGGGG	2550	0.0	17.866003	5
>>END_MODULE