FastQCFastQC Report
Mon 15 Aug 2016
SRR2921674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2275842
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18800.043.239091
GTATCAA32850.038.3485341
TCAACGC36600.033.3252834
CAACGCA36950.033.163575
ATCAACG38150.031.9713063
AACGCAG40000.030.5100696
TATCAAC42750.028.975822
GTACATG60350.028.829831
TACATGG63350.026.8205552
ACGCAGA47750.024.9614837
ACATGGG65050.024.805813
CGCAGAG50300.023.412828
CATGGGG48400.023.2393934
TAGGACG1553.934474E-521.4332564
GAGTACA43950.020.118351
ATGGGGG27050.019.6655795
GCAGAGT59050.019.38069
CCTACAC12200.019.0615433
CTACACG6950.018.4374454
TAAACCG1559.6340687E-418.3855065
Produced by FastQC (version 0.11.5)