##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921674_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2275842
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39878163774111	33.0	31.0	34.0	29.0	34.0
2	31.532528180778808	34.0	31.0	34.0	28.0	34.0
3	31.607084323076908	34.0	31.0	34.0	28.0	34.0
4	35.09725279698679	37.0	35.0	37.0	32.0	37.0
5	34.85250557815525	37.0	35.0	37.0	32.0	37.0
6	34.83157310569011	37.0	35.0	37.0	32.0	37.0
7	34.71693992816724	37.0	35.0	37.0	32.0	37.0
8	34.6630737107409	37.0	35.0	37.0	32.0	37.0
9	36.25308391355815	39.0	37.0	39.0	32.0	39.0
10-11	36.145566783634365	39.0	37.0	39.0	32.0	39.0
12-13	36.0723066451889	39.0	37.0	39.0	32.0	39.0
14-15	37.31339851360508	40.0	38.0	41.0	32.0	41.0
16-17	37.28404915631226	40.0	38.0	41.0	31.0	41.0
18-19	37.24878682263531	40.0	38.0	41.0	31.0	41.0
20-21	37.195175236242235	40.0	38.0	41.0	31.0	41.0
22-23	37.10975036931386	40.0	38.0	41.0	31.0	41.0
24-25	37.02728528606116	40.0	37.0	41.0	31.0	41.0
26-27	36.90147778272832	40.0	37.0	41.0	30.0	41.0
28-29	36.783564500523326	40.0	37.0	41.0	30.0	41.0
30-31	36.63011316251304	40.0	37.0	41.0	30.0	41.0
32-33	36.57523830740447	40.0	37.0	41.0	30.0	41.0
34-35	36.702761219803484	40.0	37.0	41.0	30.0	41.0
36-37	36.69854431898172	40.0	37.0	41.0	30.0	41.0
38-39	36.594352332016015	40.0	37.0	41.0	30.0	41.0
40-41	36.511243530965686	40.0	37.0	41.0	30.0	41.0
42-43	36.38414749354305	40.0	36.5	41.0	29.5	41.0
44-45	36.25020036540322	40.0	36.0	41.0	29.0	41.0
46-47	36.08962880551462	40.0	36.0	41.0	28.0	41.0
48-49	35.943696003501124	40.0	36.0	41.0	28.0	41.0
50-51	35.791837262867986	40.0	35.0	41.0	28.0	41.0
52-53	35.62582485954649	39.0	35.0	41.0	27.0	41.0
54-55	35.418242347227974	39.0	35.0	41.0	27.0	41.0
56-57	35.1987743437374	39.0	35.0	41.0	26.0	41.0
58-59	34.9607611161056	38.5	35.0	40.5	26.0	41.0
60-61	34.68827559206659	38.0	34.5	40.0	26.0	41.0
62-63	34.388175453304754	37.5	34.0	40.0	26.0	41.0
64-65	34.057768070015406	37.0	34.0	40.0	25.0	41.0
66-67	33.708098145653345	36.5	34.0	39.5	24.0	41.0
68-69	33.346196704340635	36.0	34.0	39.0	23.0	41.0
70-71	32.95018063644137	35.5	33.0	39.0	22.5	40.0
72-73	32.59040060777505	35.0	33.0	37.5	22.0	40.0
74-75	32.188205947513055	35.0	33.0	37.0	21.0	39.0
76-77	29.47209977669803	33.0	29.0	34.5	15.0	36.5
78-79	31.041218590745757	34.5	31.5	36.0	18.0	38.0
80-81	31.22193192673305	35.0	32.0	36.0	19.0	37.0
82-83	31.037824682029772	35.0	32.0	35.0	18.0	37.0
84-85	30.825595757526223	35.0	32.0	35.0	17.5	36.5
86-87	30.61239005168197	35.0	32.0	35.0	13.5	36.0
88-89	30.335917651576867	35.0	32.0	35.0	7.0	36.0
90-91	30.13129909721325	34.5	31.5	35.0	4.5	35.5
92-93	29.96418490387294	34.0	31.0	35.0	2.0	35.0
94-95	29.851505508730394	34.0	31.0	35.0	2.0	35.0
96-97	29.64608329576482	34.0	31.0	35.0	2.0	35.0
98-99	29.437465122798507	34.0	31.0	35.0	2.0	35.0
100-101	28.32267442115929	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	39108.0
3	13451.0
4	7235.0
5	5209.0
6	4569.0
7	4759.0
8	4982.0
9	5429.0
10	6115.0
11	6447.0
12	7105.0
13	7637.0
14	8124.0
15	9097.0
16	9826.0
17	10604.0
18	9838.0
19	8805.0
20	8354.0
21	8438.0
22	8861.0
23	10028.0
24	11793.0
25	14342.0
26	16949.0
27	20681.0
28	24901.0
29	29998.0
30	36434.0
31	45048.0
32	57307.0
33	75446.0
34	106770.0
35	158190.0
36	277877.0
37	537015.0
38	579705.0
39	79360.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.57918041387761	19.482160022817144	12.572800064789416	26.36585949851583
2	15.152943864559601	20.545909523317814	37.78727368196728	26.51387293015531
3	18.141381560589114	24.27370183444915	28.93881434249642	28.646102262465313
4	12.670864933486428	16.1192529247032	35.65578254788305	35.554099593927326
5	13.519952745232992	37.66480056044716	34.940136050834035	13.875110643485813
6	34.16187932651198	36.0073890694852	16.216098645132206	13.614632958870617
7	29.325761630201043	31.676759634456168	20.652927575815898	18.344551159526894
8	26.76442389234402	35.74813190019343	19.174617570112513	18.312826637350042
9	26.374030523221965	15.46804016625063	19.09004187902406	39.067887431503344
10-11	25.71688633347298	25.38961715506831	27.82669438567076	21.06680212578795
12-13	26.021313079938878	22.757584312553757	27.717088142470942	23.50401446503642
14-15	22.949819453736325	24.48427044918504	24.676889748685312	27.889020348393316
16-17	23.324554165007942	27.89314899716237	25.6185403028857	23.163756534943992
18-19	23.409734786443934	26.90638377806559	26.981456032638963	22.702425402851514
20-21	23.516253049156255	26.250283137899526	27.169110934912005	23.064352878032214
22-23	23.47203804130515	26.119519720613294	26.86146489958442	23.546977338497136
24-25	23.311037413863748	26.464505767682304	26.87117103559035	23.353285782863594
26-27	23.119716632670904	26.561710433461315	26.93481426538839	23.38375866847938
28-29	23.336494236332133	26.571381113941474	26.829343315810682	23.262781333915715
30-31	23.5097881825475	26.42632401563894	26.62645818551611	23.437429616297443
32-33	23.18870513125873	26.54966949114556	26.576805406265297	23.684819971330413
34-35	23.58782503764906	26.576833312752875	26.65307623721173	23.182265412386332
36-37	23.678527047715132	26.299875733602374	26.646178237034203	23.375418981648295
38-39	23.412565547168914	26.526621795361894	26.677115546685577	23.383697110783615
40-41	23.566477492308636	26.325767604013855	26.798091983514126	23.309662920163383
42-43	23.437123732886164	26.300884496872097	26.582519058341287	23.679472711900456
44-45	23.347520896848533	26.404599547743953	26.770201019289875	23.477678536117647
46-47	23.66640558143827	26.45005014813749	26.43199022742977	23.451554042994474
48-49	23.355824377819665	26.616282449844476	26.38911623575982	23.638776936576043
50-51	23.454605014400524	26.61601895257703	26.467081365972323	23.462294667050124
52-53	23.74954494054073	26.38701134523376	26.41535300859939	23.44809070562613
54-55	23.15691715088528	26.437248804842195	26.741786372861114	23.66404767141141
56-57	23.25905858597843	26.58047236586108	26.52180838850933	23.63866065965116
58-59	23.189936277741154	26.355526257965284	26.726257965282358	23.728279499011208
60-61	23.147116912267627	26.606198200842694	26.73451169355574	23.512173193333936
62-63	23.24501985509549	26.398124604870304	26.81768449439111	23.539171045643098
64-65	22.93859993261362	26.295896530041112	26.794898591853283	23.97060494549198
66-67	22.95232892612308	26.500658938202697	26.580039283488517	23.96697285218571
68-69	23.220114146042313	26.28343959194933	26.696040277528965	23.800405984479394
70-71	23.109279908049977	26.20443665882682	26.59418655318894	24.092096879934253
72-73	23.056558807718865	26.41128899332381	26.470978651057692	24.06117354789963
74-75	22.981658150326517	26.54208314397552	26.47145304614822	24.004805659549746
76-77	22.98932707198793	26.2238172612574	26.677651766464383	24.10920390029029
78-79	22.986520217914055	26.23337455429469	26.617424329045296	24.162680898745954
80-81	23.086139627975015	26.421134251681757	26.36096285663973	24.1317632637035
82-83	23.1498585866577	26.40236545954392	26.46077675151467	23.98699920228371
84-85	22.93842702477664	26.420692465719398	26.447839105492942	24.19304140401102
86-87	22.900060018071375	26.502287501951137	26.439763181441446	24.15788929853604
88-89	22.796968380307074	26.36025403629871	26.349039035254325	24.493738548139895
90-91	22.77601423992547	26.399015826889183	26.438568727075296	24.386401206110055
92-93	22.887898251583774	26.195974729956077	26.636595841961107	24.279531176499034
94-95	22.685666123523713	25.888210106677494	26.762885583157995	24.663238186640793
96-97	22.743514854704973	25.70823745730624	26.78839186678315	24.75985582120564
98-99	22.52352720829502	25.830595312244252	26.694656547248304	24.95122093221242
100-101	22.489684024221397	25.827595888812873	26.728780023542964	24.953940063422763
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	22.0
2	28.5
3	45.5
4	46.0
5	46.5
6	94.0
7	202.5
8	308.0
9	351.0
10	314.5
11	266.5
12	259.5
13	292.5
14	334.5
15	367.0
16	398.5
17	444.5
18	523.5
19	617.0
20	713.5
21	845.0
22	1015.5
23	1236.5
24	1612.5
25	2217.0
26	2990.5
27	4142.0
28	5830.0
29	8250.5
30	11841.0
31	16782.0
32	24914.5
33	30939.5
34	34811.5
35	41328.0
36	49836.5
37	57573.0
38	63063.5
39	68629.5
40	72232.0
41	74263.0
42	76914.5
43	80081.5
44	83614.0
45	88522.0
46	91045.5
47	91614.0
48	93145.5
49	93445.0
50	92755.0
51	91551.5
52	90254.5
53	89743.0
54	88434.5
55	83899.5
56	77310.0
57	70024.5
58	63282.5
59	55110.0
60	45199.5
61	37272.0
62	30390.5
63	22828.5
64	15950.5
65	11311.0
66	8399.0
67	6496.5
68	4908.5
69	3509.5
70	2557.0
71	1830.5
72	1310.5
73	955.5
74	720.0
75	535.0
76	361.5
77	214.0
78	107.5
79	64.5
80	45.5
81	31.0
82	17.0
83	9.5
84	5.5
85	7.0
86	6.5
87	2.5
88	1.0
89	0.0
90	0.0
91	1.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16987119492477948
2	3.0757846985862816E-4
3	0.009974330379701227
4	0.006195509178580938
5	0.022672927206721732
6	0.0021091095076020217
7	0.0
8	0.0
9	4.393978140837545E-5
10-11	0.04872921758188837
12-13	4.1742792337956676E-4
14-15	0.0048114060642171115
16-17	0.0
18-19	0.001515922458588953
20-21	3.2954836056281586E-4
22-23	0.0
24-25	6.371268304214441E-4
26-27	0.0029879051357695305
28-29	0.005316713550413429
30-31	0.016191809448986353
32-33	0.011424343166177617
34-35	0.009710691691250975
36-37	0.0037788212011202887
38-39	0.0
40-41	0.003317453496332346
42-43	0.008700076718858338
44-45	0.006151569397172564
46-47	0.0037129115290077254
48-49	0.0085902272653374
50-51	0.002482597649573213
52-53	0.0017795611470392055
54-55	0.004240188905908231
56-57	0.007447792948719638
58-59	0.015027405241664404
60-61	0.007272033823086136
62-63	0.021157004748132778
64-65	0.03961171293965047
66-67	0.05929673501060267
68-69	0.04941028419371819
70-71	0.06867787834129083
72-73	0.03255937802360621
74-75	0.026715387096292273
76-77	0.2280694354001728
78-79	0.08427650074126411
80-81	0.06615134091030925
82-83	0.02623204950080014
84-85	0.05092620665230715
86-87	0.06709604621058932
88-89	0.28824496603894295
90-91	0.2956707890969584
92-93	0.053255015066951045
94-95	0.008700076718858338
96-97	0.0146758869903974
98-99	8.568257374633213E-4
100-101	1.3181934422512636E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2275842.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.394068991475066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.05329854344109	46.16243769149374
2	11.843929785420237	13.832305060400316
3	3.7243146870882633	6.5243366635138775
4	1.7147637560737208	4.005281323049991
5	0.9161195156205797	2.6747973099792426
6	0.5652913394126171	1.9805796884366227
7	0.3948948974920417	1.6141663921972231
8	0.2828529306537728	1.3213546837629857
9	0.21834879130466045	1.1475246945284578
>10	1.1681139136662368	12.268711013433542
>50	0.07804269014185986	3.1074058263073674
>100	0.037388592228966774	3.9034481408389032
>500	0.0017352234710069298	0.7180102258747527
>1k	9.053339848730215E-4	0.7396412861829458
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.393978140837545E-5	0.0
3	0.0	0.0	0.0	4.393978140837545E-5	0.0
4	0.0	0.0	0.0	4.393978140837545E-5	0.0
5	0.0	0.0	0.0	4.393978140837545E-5	0.0
6	0.0	0.0	0.0	4.393978140837545E-5	0.0
7	0.0	0.0	0.0	4.393978140837545E-5	0.0
8	0.0	0.0	0.0	4.393978140837545E-5	0.0
9	0.0	0.0	0.0	4.393978140837545E-5	0.0
10-11	0.0	0.0	0.0	4.393978140837545E-5	0.0
12-13	0.0	0.0	0.0	4.393978140837545E-5	8.78795628167509E-5
14-15	0.0	0.0	0.0	4.393978140837545E-5	1.0984945352093863E-4
16-17	0.0	0.0	0.0	4.393978140837545E-5	1.5378923492931408E-4
18-19	0.0	0.0	0.0	4.393978140837545E-5	2.1969890704187726E-4
20-21	0.0	0.0	0.0	6.590967211256318E-5	3.2954836056281586E-4
22-23	0.0	0.0	0.0	8.78795628167509E-5	4.1742792337956676E-4
24-25	2.1969890704187726E-5	0.0	0.0	1.3181934422512636E-4	4.8333759549212997E-4
26-27	4.393978140837545E-5	0.0	0.0	3.7348814197119137E-4	5.272773769005054E-4
28-29	4.393978140837545E-5	0.0	0.0	0.002636386884502527	5.712171583088808E-4
30-31	4.393978140837545E-5	0.0	0.0	0.012412988247866064	5.712171583088808E-4
32-33	4.393978140837545E-5	0.0	0.0	0.03398742091937841	6.151569397172563E-4
34-35	4.393978140837545E-5	0.0	0.0	0.0633611647908774	6.590967211256317E-4
36-37	4.393978140837545E-5	0.0	0.0	0.10890474822065854	6.590967211256317E-4
38-39	8.78795628167509E-5	0.0	0.0	0.18441526257095175	7.030365025340072E-4
40-41	8.78795628167509E-5	0.0	0.0	0.27264634363896967	7.909160653507581E-4
42-43	8.78795628167509E-5	0.0	0.0	0.36237137727487234	8.128859560549459E-4
44-45	8.78795628167509E-5	0.0	0.0	0.46958444391130844	8.348558467591335E-4
46-47	8.78795628167509E-5	0.0	0.0	0.5972734486840475	8.348558467591335E-4
48-49	8.78795628167509E-5	0.0	0.0	0.739418641540142	9.227354095758844E-4
50-51	8.78795628167509E-5	0.0	0.0	0.8914063454317127	9.227354095758844E-4
52-53	8.78795628167509E-5	0.0	0.0	1.044514513749197	0.0010325848630968232
54-55	8.78795628167509E-5	0.0	0.0	1.2166266375258035	0.0010545547538010108
56-57	8.78795628167509E-5	0.0	0.0	1.4154761182894067	0.0010545547538010108
58-59	8.78795628167509E-5	0.0	0.0	1.6298143719994622	0.0010545547538010108
60-61	8.78795628167509E-5	0.0	0.0	1.8656611487089174	0.0010545547538010108
62-63	8.78795628167509E-5	0.0	0.0	2.111240587000328	0.0010984945352093863
64-65	8.78795628167509E-5	0.0	0.0	2.3700458994956595	0.001120464425913574
66-67	8.78795628167509E-5	0.0	0.0	2.6647719832923373	0.0011424343166177616
68-69	1.3181934422512636E-4	0.0	0.0	2.9835111576286932	0.0011424343166177616
70-71	1.3181934422512636E-4	0.0	0.0	3.332239232776265	0.0012303138794345126
72-73	1.3181934422512636E-4	0.0	0.0	3.6969833582471896	0.0013181934422512634
74-75	1.3181934422512636E-4	0.0	0.0	4.064297960930504	0.001362133223659639
76-77	1.3181934422512636E-4	0.0	0.0	4.472081102290932	0.0014060730050680145
78-79	1.3181934422512636E-4	0.0	0.0	4.934371542488451	0.001428042895772202
80-81	1.3181934422512636E-4	0.0	0.0	5.431440319670697	0.0014500127864763897
82-83	1.3181934422512636E-4	0.0	0.0	5.942767555919962	0.0016257719121098916
84-85	1.3181934422512636E-4	0.0	0.0	6.4609493980689345	0.0016257719121098916
86-87	1.3181934422512636E-4	0.0	0.0	7.007560278789125	0.0016257719121098916
88-89	1.3181934422512636E-4	0.0	0.0	7.6083049702044345	0.001669711693518267
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1880	0.0	43.23909	1
GTATCAA	3285	0.0	38.348534	1
TCAACGC	3660	0.0	33.325283	4
CAACGCA	3695	0.0	33.16357	5
ATCAACG	3815	0.0	31.971306	3
AACGCAG	4000	0.0	30.510069	6
TATCAAC	4275	0.0	28.97582	2
GTACATG	6035	0.0	28.82983	1
TACATGG	6335	0.0	26.820555	2
ACGCAGA	4775	0.0	24.961483	7
ACATGGG	6505	0.0	24.80581	3
CGCAGAG	5030	0.0	23.41282	8
CATGGGG	4840	0.0	23.239393	4
TAGGACG	155	3.934474E-5	21.433256	4
GAGTACA	4395	0.0	20.11835	1
ATGGGGG	2705	0.0	19.665579	5
GCAGAGT	5905	0.0	19.3806	9
CCTACAC	1220	0.0	19.061543	3
CTACACG	695	0.0	18.437445	4
TAAACCG	155	9.6340687E-4	18.385506	5
>>END_MODULE