FastQCFastQC Report
Mon 15 Aug 2016
SRR2921673_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921673_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336287
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.048.5624241
GTATCAA27500.040.389131
TCAACGC31600.033.799064
CAACGCA32450.032.619065
ATCAACG33850.031.8322323
AACGCAG34050.031.065676
TATCAAC35450.030.3955152
GTACATG55150.029.9512561
TACATGG54950.028.4202632
ACATGGG55650.027.2098033
ACGCAGA40600.025.8262627
TATACCG2059.658834E-1025.4694845
CATGGGG49350.024.0470644
CGCAGAG43250.024.0259848
GTGTAGC12750.023.8259961
GAGTACA36850.021.124561
TAAGGTG10800.020.2169385
GCAGAGT52050.019.326259
ATGGGGG29600.019.2429145
AGAGTAC41550.018.2723910-11
Produced by FastQC (version 0.11.5)