##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921673_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2336287
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.925040887527945	31.0	31.0	34.0	26.0	34.0
2	29.978541163821056	31.0	31.0	34.0	25.0	34.0
3	30.041141349500297	31.0	31.0	34.0	25.0	34.0
4	33.479133770808126	37.0	35.0	37.0	28.0	37.0
5	33.470852254025296	37.0	35.0	37.0	28.0	37.0
6	33.5085518174779	37.0	35.0	37.0	28.0	37.0
7	33.40499775926502	37.0	35.0	37.0	27.0	37.0
8	33.37241828593833	37.0	35.0	37.0	27.0	37.0
9	34.87695176149163	39.0	35.0	39.0	27.0	39.0
10-11	34.76592409237392	39.0	35.0	39.0	27.0	39.0
12-13	34.643137165938946	39.0	35.0	39.0	27.0	39.0
14-15	35.734500941023086	40.0	36.0	41.0	27.0	41.0
16-17	35.66939849427746	40.0	36.0	41.0	27.0	41.0
18-19	35.628953334928454	40.0	36.0	41.0	26.0	41.0
20-21	35.54797270198396	40.0	36.0	41.0	25.5	41.0
22-23	35.449336061879386	40.0	36.0	41.0	25.0	41.0
24-25	35.338065913990874	40.0	36.0	41.0	25.0	41.0
26-27	35.18978811250501	40.0	35.0	41.0	24.0	41.0
28-29	35.04425719100436	39.0	35.0	41.0	24.0	41.0
30-31	34.89224804144354	39.0	35.0	41.0	23.0	41.0
32-33	34.7141588340816	39.0	35.0	41.0	22.0	41.0
34-35	34.533945101779025	39.0	34.0	41.0	20.5	41.0
36-37	34.50567117824137	39.0	34.5	41.0	20.0	41.0
38-39	34.68026081555905	39.0	35.0	41.0	21.5	41.0
40-41	34.73982477324061	39.5	35.0	41.0	20.5	41.0
42-43	34.577312419236165	39.0	35.0	41.0	18.0	41.0
44-45	34.45551317111297	39.0	35.0	41.0	16.5	41.0
46-47	34.290897265618476	39.0	34.0	41.0	15.0	41.0
48-49	34.12239420927309	39.0	34.0	41.0	13.5	41.0
50-51	32.46440655621505	37.0	32.0	39.5	8.5	40.0
52-53	32.576794717429834	37.5	32.0	39.5	8.0	40.0
54-55	33.48030186359809	38.0	33.0	40.0	8.0	41.0
56-57	33.50784984892695	38.0	33.0	41.0	7.0	41.0
58-59	33.32648107873733	38.0	33.0	40.0	2.0	41.0
60-61	33.080485830722	37.5	33.0	40.0	2.0	41.0
62-63	32.7941299592045	37.0	33.0	40.0	2.0	41.0
64-65	32.51312938008044	37.0	32.5	40.0	2.0	41.0
66-67	32.179618557137886	36.0	32.0	39.0	2.0	41.0
68-69	31.82834193744176	36.0	32.0	39.0	2.0	41.0
70-71	31.42389355417378	35.0	31.5	39.0	2.0	40.5
72-73	31.07142487202985	35.0	31.0	37.5	2.0	40.0
74-75	30.67848192452383	35.0	31.0	37.0	2.0	39.0
76-77	30.311603839767976	35.0	31.0	36.5	2.0	39.0
78-79	29.965973786610977	35.0	31.0	36.0	2.0	38.5
80-81	29.65022961648119	35.0	30.0	36.0	2.0	37.0
82-83	29.335928334147304	35.0	30.0	35.0	2.0	37.0
84-85	29.072563430777127	35.0	30.0	35.0	2.0	36.5
86-87	28.841873023305784	34.0	30.0	35.0	2.0	36.0
88-89	28.617124522800495	34.0	29.5	35.0	2.0	36.0
90-91	28.390330040786942	34.0	29.0	35.0	2.0	35.5
92-93	28.194707884776143	34.0	29.0	35.0	2.0	35.0
94-95	27.964372313846717	34.0	29.0	35.0	2.0	35.0
96-97	27.729424509916804	34.0	29.0	35.0	2.0	35.0
98-99	27.45668361806576	34.0	29.0	35.0	2.0	35.0
100-101	26.400901729967252	33.0	25.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	100386.0
3	16398.0
4	11014.0
5	8193.0
6	7416.0
7	7440.0
8	8016.0
9	8441.0
10	9312.0
11	9712.0
12	9745.0
13	9884.0
14	10431.0
15	10878.0
16	11315.0
17	11763.0
18	11215.0
19	10429.0
20	10738.0
21	11120.0
22	12281.0
23	13884.0
24	16424.0
25	19259.0
26	22937.0
27	26644.0
28	31671.0
29	37010.0
30	43673.0
31	52947.0
32	66054.0
33	84801.0
34	115078.0
35	165626.0
36	271877.0
37	475116.0
38	494992.0
39	92153.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.43033316945236	17.318935790063332	11.733264012251674	25.517467028232637
2	16.11182930559018	18.63761791311246	39.43286052434765	25.81769225694971
3	20.053207483485636	22.300369137089582	28.87000703322234	28.776416346202442
4	13.075394590251074	14.531295443360145	36.16635048617837	36.22695948021041
5	13.98982150367966	37.12722606421481	34.76185214025115	14.121100291854377
6	35.146030130232155	34.787263506615204	16.13199837846561	13.93470798468703
7	29.88150583481023	31.209727823012184	20.092723396604423	18.816042945573162
8	27.067484736115237	35.51474245967089	18.88903063736298	18.528742166850893
9	26.592807507026496	15.212541275033622	18.70365788615325	39.49099333178663
10-11	26.184605854841088	25.00720783853243	27.47818545260196	21.330000854024522
12-13	26.359068106726546	22.143393059927455	27.501554618206104	23.995984215139895
14-15	23.06436550188255	23.997761408445758	24.45470727246802	28.48316581720367
16-17	23.775023783244094	27.299974568412257	25.39765755382377	23.52734409451988
18-19	23.77584390183764	26.5737926777404	26.55213235399561	23.098231066426347
20-21	24.014224442314827	25.782047859901276	26.843914936756114	23.359812761027783
22-23	23.760442843801904	25.751343102467146	26.534660757172812	23.953553296558137
24-25	23.618841688922668	25.947516872635024	26.620730995873497	23.812910442568818
26-27	23.56721547288916	26.14394684538991	26.443091366978084	23.84574631474285
28-29	23.69671715701551	26.37943788248751	26.2871443101612	23.636700650335783
30-31	23.852952199447824	26.08492315700636	26.271757270188584	23.79036737335723
32-33	23.54377332281722	26.11543396626211	26.286103212729778	24.054689498190893
34-35	23.960558529439524	26.201490858650946	26.307882922072682	23.53006768983685
36-37	24.000898866785803	25.94300756546211	26.347454815892824	23.70863875185926
38-39	23.736318111949746	26.09984808722165	26.364700247280958	23.799133553547648
40-41	23.996015866337196	25.92255080433872	26.42724483117726	23.65418849814683
42-43	23.71768980713358	25.96130230682524	26.341737031146266	23.979270854894914
44-45	23.762109149173053	26.07790083492444	26.480181051829817	23.679808964072688
46-47	23.963104092428054	26.059380597717475	26.161135347112364	23.816379962742108
48-49	23.692803063528192	26.227149480608357	26.16146016853608	23.918587287327366
50-51	23.790348521329786	26.305035721913754	26.251918192571523	23.652697564184933
52-53	23.991217431827973	26.115358492500224	26.239766770400998	23.653657305270805
54-55	23.476564845552726	26.076820470590256	26.61491975260986	23.831694931247156
56-57	23.638213426430596	26.22967027004654	26.357572491328963	23.7745438121939
58-59	23.6623493665174	26.055262785950806	26.459525766858928	23.822862080672866
60-61	23.563553799076303	26.161853328691905	26.59757714821066	23.677015724021132
62-63	23.638076929840476	26.106272311270722	26.595399272828963	23.66025148605984
64-65	23.333953818730198	26.047240669986977	26.62620072502432	23.992604786258507
66-67	23.381530935998516	26.086022266327408	26.474562495509957	24.05788430216412
68-69	23.725024526491293	25.900510901556295	26.564822310261455	23.80964226169096
70-71	23.519650473370916	25.80010330899704	26.48282611217453	24.197420105457514
72-73	23.497336898003525	25.980775742515128	26.453436942372477	24.06845041710887
74-75	23.405812547633374	26.098092692134323	26.499446091136132	23.996648669096174
76-77	23.562826255144174	25.750526211057377	26.576081455927785	24.11056607787066
78-79	23.449654382574792	25.80911795173152	26.577947828175702	24.16327983751799
80-81	23.544132257782216	25.937975637085582	26.40732809285294	24.110564012279266
82-83	23.52637106004163	25.99761230243368	26.43150319417824	24.04451344334645
84-85	23.42795410280349	25.99075990663392	26.505976291295646	24.07530969926694
86-87	23.39059011749321	26.067249853109352	26.448940295789175	24.093219733608258
88-89	23.329538088372008	25.9289519847172	26.44892485457877	24.29258507233202
90-91	23.347640000549156	25.870841856697645	26.5630791883469	24.21843895440629
92-93	23.40892291072928	25.649710994066293	26.76999778199261	24.171368313211815
94-95	23.30472552290341	25.486500099535274	26.839579171729238	24.36919520583208
96-97	23.323473621839305	25.3053700245034	26.90469846573793	24.466457887919365
98-99	23.15809231660294	25.31332621858023	26.890978028283826	24.637603436533006
100-101	23.333188884164496	25.240218252682784	26.920576841308574	24.506016021844147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	14.5
2	20.5
3	36.0
4	39.5
5	44.5
6	74.0
7	150.0
8	259.0
9	325.5
10	331.0
11	334.0
12	369.0
13	422.0
14	479.5
15	559.0
16	636.0
17	706.0
18	808.0
19	923.5
20	1033.0
21	1198.0
22	1379.5
23	1641.5
24	2039.5
25	2565.0
26	3478.5
27	5050.0
28	7091.0
29	9678.5
30	13157.5
31	18031.5
32	24754.0
33	29129.0
34	31453.5
35	35755.5
36	42115.0
37	48466.5
38	53957.5
39	59254.0
40	64068.5
41	69292.0
42	73993.0
43	78451.5
44	83097.0
45	88323.0
46	92619.0
47	95783.0
48	98324.5
49	99041.0
50	99199.5
51	99665.0
52	99560.0
53	97998.0
54	95500.0
55	90813.5
56	84026.5
57	76300.0
58	68584.0
59	59704.5
60	49368.5
61	40674.0
62	33435.0
63	25796.5
64	18693.5
65	13729.0
66	10323.5
67	7975.0
68	6179.0
69	4673.5
70	3578.0
71	2593.5
72	1811.5
73	1335.5
74	1042.0
75	779.0
76	557.0
77	376.5
78	257.5
79	190.0
80	145.5
81	111.0
82	74.0
83	62.5
84	59.0
85	55.0
86	52.0
87	44.0
88	33.5
89	30.5
90	26.0
91	15.5
92	13.0
93	14.0
94	14.0
95	16.0
96	12.0
97	9.5
98	8.0
99	4.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05388892717375904
2	0.07400631857301779
3	0.07075329358079722
4	0.07062488469952535
5	0.06681542122179339
6	0.009759074976661685
7	0.013140508850154112
8	0.016307927921526764
9	0.005521581894690164
10-11	0.012348654082310949
12-13	0.021594093533885177
14-15	0.03809463477731974
16-17	0.02606700289818845
18-19	0.009352446852634116
20-21	0.019197127750143712
22-23	0.03578327491442618
24-25	0.033150892848352966
26-27	0.019667960314807213
28-29	0.011257178591500102
30-31	0.011021762309168352
32-33	0.008111161000339427
34-35	0.00498654488939073
36-37	5.136355250874572E-4
38-39	0.0033386309130684715
40-41	8.560592084790953E-4
42-43	0.2255501999540296
44-45	0.19599475578128883
46-47	0.20119531547279937
48-49	0.20791538025936024
50-51	0.19967581037774895
52-53	0.20761575953639255
54-55	0.20318565313251327
56-57	0.20624606480282603
58-59	0.21433582432295348
60-61	0.2186589233257729
62-63	0.20485496858904748
64-65	0.2045553478660798
66-67	0.2028646309293336
68-69	0.1979422904805788
70-71	0.1903661664855388
72-73	0.2012381184332233
74-75	0.20014664294241247
76-77	0.1935977899975474
78-79	0.21196026001942397
80-81	0.22270380308583665
82-83	0.22133410835227008
84-85	0.2154487012939763
86-87	0.23419639795966848
88-89	0.22685569024696023
90-91	0.23280530174588995
92-93	0.22968068563494126
94-95	0.23349014911267324
96-97	0.2392685487699071
98-99	0.23325473283034148
100-101	0.23943976061160294
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2336287.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.32689094398341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.13636983806242	51.54923523219763
2	11.467667373499427	14.753587770339488
3	3.5872919920401847	6.922780222685819
4	1.607553160101793	4.136355872660957
5	0.8848202083769146	2.8458866524647233
6	0.5687092850366702	2.1949980094430797
7	0.3605736971752007	1.6236209426820625
8	0.2509652316081393	1.2915050467510656
9	0.19561789936480165	1.1325142151217646
>10	0.8954685309581479	9.868935527888729
>50	0.033608478351208795	1.4155481882667376
>100	0.009487526628549353	1.113511804423914
>500	0.001133401412171778	0.517047721778531
>1k	7.333773843436918E-4	0.634472793295522
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.280296042395476E-5	0.0
5	0.0	0.0	0.0	4.280296042395476E-5	0.0
6	0.0	0.0	0.0	4.280296042395476E-5	0.0
7	0.0	0.0	0.0	4.280296042395476E-5	0.0
8	0.0	0.0	0.0	4.280296042395476E-5	0.0
9	0.0	0.0	0.0	4.280296042395476E-5	0.0
10-11	0.0	0.0	0.0	1.284088812718643E-4	1.4981036148384167E-4
12-13	0.0	0.0	0.0	1.284088812718643E-4	2.1401480211977381E-4
14-15	0.0	0.0	0.0	1.284088812718643E-4	2.1401480211977381E-4
16-17	0.0	0.0	0.0	1.284088812718643E-4	2.1401480211977381E-4
18-19	0.0	0.0	0.0	1.284088812718643E-4	2.1401480211977381E-4
20-21	0.0	2.140148021197738E-5	0.0	1.284088812718643E-4	2.1401480211977381E-4
22-23	0.0	4.280296042395476E-5	0.0	1.284088812718643E-4	2.1401480211977381E-4
24-25	0.0	4.280296042395476E-5	0.0	1.7121184169581905E-4	2.1401480211977381E-4
26-27	0.0	4.280296042395476E-5	0.0	3.424236833916381E-4	2.354162823317512E-4
28-29	0.0	4.280296042395476E-5	0.0	0.002717987986921127	2.568177625437286E-4
30-31	0.0	4.280296042395476E-5	0.0	0.01200623039891931	2.568177625437286E-4
32-33	0.0	4.280296042395476E-5	0.0	0.029298626410197037	2.568177625437286E-4
34-35	0.0	4.280296042395476E-5	0.0	0.05046469033984266	2.568177625437286E-4
36-37	0.0	4.280296042395476E-5	0.0	0.08785307627016714	2.568177625437286E-4
38-39	0.0	8.560592084790953E-5	0.0	0.15143687397995195	2.568177625437286E-4
40-41	2.140148021197738E-5	8.560592084790953E-5	0.0	0.2281183775794669	2.568177625437286E-4
42-43	4.280296042395476E-5	8.560592084790953E-5	0.0	0.30899457130052943	2.7821924275570596E-4
44-45	4.280296042395476E-5	8.560592084790953E-5	0.0	0.4005287021671567	2.9962072296768334E-4
46-47	4.280296042395476E-5	8.560592084790953E-5	0.0	0.5080283372719191	2.9962072296768334E-4
48-49	4.280296042395476E-5	8.560592084790953E-5	0.0	0.6297599567176464	2.9962072296768334E-4
50-51	4.280296042395476E-5	8.560592084790953E-5	0.0	0.7642468583697123	3.424236833916381E-4
52-53	4.280296042395476E-5	1.284088812718643E-4	0.0	0.9014731494889112	4.0662812402757025E-4
54-55	4.280296042395476E-5	1.284088812718643E-4	0.0	1.047324237133537	4.2802960423954763E-4
56-57	4.280296042395476E-5	1.4981036148384167E-4	0.0	1.215839492322647	4.2802960423954763E-4
58-59	4.280296042395476E-5	1.7121184169581905E-4	0.0	1.4035946782223245	4.2802960423954763E-4
60-61	4.280296042395476E-5	1.7121184169581905E-4	0.0	1.6112746421993531	4.2802960423954763E-4
62-63	4.280296042395476E-5	1.7121184169581905E-4	0.0	1.8182483573293864	4.2802960423954763E-4
64-65	4.280296042395476E-5	1.7121184169581905E-4	0.0	2.0318351298449207	4.708325646635024E-4
66-67	4.280296042395476E-5	1.7121184169581905E-4	0.0	2.2786369996494438	4.708325646635024E-4
68-69	4.280296042395476E-5	1.7121184169581905E-4	0.0	2.556663629083242	4.708325646635024E-4
70-71	4.280296042395476E-5	1.7121184169581905E-4	0.0	2.854636438074603	4.708325646635024E-4
72-73	4.280296042395476E-5	1.7121184169581905E-4	0.0	3.165086310029547	4.922340448754798E-4
74-75	4.280296042395476E-5	1.7121184169581905E-4	0.0	3.48018030319049	5.136355250874572E-4
76-77	4.280296042395476E-5	1.7121184169581905E-4	0.0	3.8197789911941467	5.136355250874572E-4
78-79	4.280296042395476E-5	1.7121184169581905E-4	0.0	4.209842369537647	5.136355250874572E-4
80-81	4.280296042395476E-5	1.7121184169581905E-4	0.0	4.6342551236213705	5.136355250874572E-4
82-83	4.280296042395476E-5	1.9261332190779643E-4	0.0	5.070181874059138	5.136355250874572E-4
84-85	4.280296042395476E-5	2.1401480211977381E-4	0.0	5.506301237818812	5.136355250874572E-4
86-87	6.420444063593214E-5	2.1401480211977381E-4	0.0	5.966433062376327	5.136355250874572E-4
88-89	8.560592084790953E-5	2.1401480211977381E-4	0.0	6.4787630971708525	5.136355250874572E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1515	0.0	48.562424	1
GTATCAA	2750	0.0	40.38913	1
TCAACGC	3160	0.0	33.79906	4
CAACGCA	3245	0.0	32.61906	5
ATCAACG	3385	0.0	31.832232	3
AACGCAG	3405	0.0	31.06567	6
TATCAAC	3545	0.0	30.395515	2
GTACATG	5515	0.0	29.951256	1
TACATGG	5495	0.0	28.420263	2
ACATGGG	5565	0.0	27.209803	3
ACGCAGA	4060	0.0	25.826262	7
TATACCG	205	9.658834E-10	25.469484	5
CATGGGG	4935	0.0	24.047064	4
CGCAGAG	4325	0.0	24.025984	8
GTGTAGC	1275	0.0	23.825996	1
GAGTACA	3685	0.0	21.12456	1
TAAGGTG	1080	0.0	20.216938	5
GCAGAGT	5205	0.0	19.32625	9
ATGGGGG	2960	0.0	19.242914	5
AGAGTAC	4155	0.0	18.27239	10-11
>>END_MODULE