FastQCFastQC Report
Mon 15 Aug 2016
SRR2921673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336287
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16000.041.9014051
GTATCAA27000.039.975291
TCAACGC30300.033.8395044
ATCAACG31850.032.343113
CAACGCA31750.032.1672975
AACGCAG33150.030.9434786
TATCAAC34600.030.458492
GTACATG51550.029.6077821
TACATGG53700.026.9623052
ACATGGG54250.025.2013743
ACGCAGA41200.024.6664247
GTATAGC7700.023.46511
CGCAGAG43450.022.9519238
CATGGGG45100.022.8399984
GAGTACA40100.021.5802521
TATACCG2401.4138459E-719.7928625
TAGACCG1507.721176E-419.0011485
GTGTAGC13550.018.9488961
AGTACAT42000.018.875512
TAGAGCG2803.4904588E-818.6618425
Produced by FastQC (version 0.11.5)