##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921673_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2336287
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43755155081546	33.0	31.0	34.0	29.0	34.0
2	31.57146360870903	34.0	31.0	34.0	29.0	34.0
3	31.646863591673455	34.0	31.0	34.0	28.0	34.0
4	35.13267890460376	37.0	35.0	37.0	33.0	37.0
5	34.89046208791985	37.0	35.0	37.0	32.0	37.0
6	34.87360970634173	37.0	35.0	37.0	32.0	37.0
7	34.756226011615865	37.0	35.0	37.0	32.0	37.0
8	34.70987511380237	37.0	35.0	37.0	32.0	37.0
9	36.29954838596457	39.0	37.0	39.0	32.0	39.0
10-11	36.19619699976929	39.0	37.0	39.0	32.0	39.0
12-13	36.117382196622245	39.0	37.0	39.0	32.0	39.0
14-15	37.37620335172862	40.0	38.0	41.0	32.0	41.0
16-17	37.33977696233383	40.0	38.0	41.0	32.0	41.0
18-19	37.30054783509047	40.0	38.0	41.0	31.0	41.0
20-21	37.24329138500535	40.0	38.0	41.0	31.0	41.0
22-23	37.155565647542446	40.0	38.0	41.0	31.0	41.0
24-25	37.07856055356213	40.0	37.5	41.0	31.0	41.0
26-27	36.95419655205033	40.0	37.0	41.0	30.5	41.0
28-29	36.83645759275295	40.0	37.0	41.0	30.0	41.0
30-31	36.68035883433842	40.0	37.0	41.0	30.0	41.0
32-33	36.63565392436803	40.0	37.0	41.0	30.0	41.0
34-35	36.7673552093557	40.0	37.0	41.0	30.0	41.0
36-37	36.76477718704936	40.0	37.0	41.0	30.0	41.0
38-39	36.66181166954232	40.0	37.0	41.0	30.0	41.0
40-41	36.5807084061162	40.0	37.0	41.0	30.0	41.0
42-43	36.45774620155828	40.0	37.0	41.0	30.0	41.0
44-45	36.33447046531526	40.0	36.5	41.0	29.5	41.0
46-47	36.17606783755592	40.0	36.0	41.0	28.5	41.0
48-49	36.029678930713565	40.0	36.0	41.0	28.0	41.0
50-51	35.87642014872317	40.0	35.5	41.0	28.0	41.0
52-53	35.70595436262754	39.0	35.0	41.0	28.0	41.0
54-55	35.498507032740406	39.0	35.0	41.0	27.0	41.0
56-57	35.27447548182222	39.0	35.0	41.0	27.0	41.0
58-59	35.032333570319054	39.0	35.0	40.5	26.0	41.0
60-61	34.762636396983766	38.0	35.0	40.0	26.0	41.0
62-63	34.460533530341095	37.5	34.0	40.0	26.0	41.0
64-65	34.125347613542345	37.0	34.0	40.0	26.0	41.0
66-67	33.778011862412455	36.5	34.0	39.5	25.0	41.0
68-69	33.41750885058214	36.0	34.0	39.0	24.0	41.0
70-71	33.02180318599555	35.5	33.0	39.0	23.0	40.0
72-73	32.659930265416875	35.0	33.0	37.5	22.5	40.0
74-75	32.25864694705744	35.0	33.0	37.0	21.5	39.0
76-77	29.551150179751033	33.0	29.0	34.5	16.5	36.5
78-79	31.115965418632214	34.5	31.5	36.0	19.0	38.0
80-81	31.295013412307647	35.0	32.0	36.0	20.0	37.0
82-83	31.11694731854434	35.0	32.0	35.0	19.0	37.0
84-85	30.910007845782644	35.0	32.0	35.0	18.0	36.5
86-87	30.69494993551734	35.0	32.0	35.0	17.5	36.0
88-89	30.413826083867264	35.0	32.0	35.0	10.0	36.0
90-91	30.210919506036717	34.5	31.5	35.0	4.5	35.5
92-93	30.04747383348022	34.0	31.0	35.0	2.0	35.0
94-95	29.939350131212475	34.0	31.0	35.0	2.0	35.0
96-97	29.734667444539134	34.0	31.0	35.0	2.0	35.0
98-99	29.53082968830456	34.0	31.0	35.0	2.0	35.0
100-101	28.416281261677184	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	39067.0
3	13486.0
4	7556.0
5	5443.0
6	4764.0
7	4763.0
8	4930.0
9	5342.0
10	5946.0
11	6590.0
12	7057.0
13	7613.0
14	8161.0
15	9030.0
16	9746.0
17	10472.0
18	9670.0
19	8801.0
20	8473.0
21	8408.0
22	8922.0
23	10157.0
24	12042.0
25	14656.0
26	17288.0
27	21026.0
28	25109.0
29	30196.0
30	36848.0
31	45865.0
32	58162.0
33	76673.0
34	108773.0
35	162426.0
36	285816.0
37	555719.0
38	598025.0
39	83265.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11327598292778	18.83071197820313	12.392275059004607	26.663736979864478
2	15.249394016050283	19.96157999694386	37.77624542917104	27.012780557834816
3	18.47400475080939	23.391507703477235	28.832806868767985	29.30168067694539
4	12.861806804936482	15.668553098100855	34.984934659785274	36.48470543717738
5	13.642633840842361	37.38450038146663	34.96345027361989	14.009415504071118
6	35.19256723063337	35.406927943040316	15.801055452565201	13.599449373761102
7	29.71180338716947	31.356935171064173	20.159252694553366	18.772008747212993
8	27.005629017325354	36.08726153935711	18.567410596386487	18.33969884693105
9	26.31385883406398	15.19959457018533	18.5871507169927	39.89939587875799
10-11	26.121071984436213	25.183900320512752	27.4539973522208	21.241030342830236
12-13	26.269454003800913	22.267086992997413	27.528528258599138	23.934930744602532
14-15	22.952067681443143	24.141916321070365	24.37732203368791	28.528693963798585
16-17	23.688549394830343	27.59346347430774	25.249915785175364	23.468071345686553
18-19	23.689231498208564	26.72828526527622	26.568799147866446	23.01368408864877
20-21	23.936728474326706	25.937687263512938	26.768923245501398	23.35666101665896
22-23	23.74175347463732	25.83787865103902	26.497707687454497	23.922660186869166
24-25	23.593143264140476	26.068197538642764	26.571071347856755	23.767587849360005
26-27	23.461214796856126	26.275304700829455	26.4586773711105	23.804803131203915
28-29	23.617224237279828	26.470194804597195	26.27125735956183	23.641323598561144
30-31	23.77529842682699	26.23668830557263	26.24373051085741	23.744282756742965
32-33	23.469830902886507	26.25276404421272	26.290563305307803	23.986841747592972
34-35	23.834320321977756	26.300008304574106	26.337036431396083	23.52863494205205
36-37	23.88692154484995	26.04420595659363	26.403314612056068	23.66555788650035
38-39	23.70442929314763	26.203608546381503	26.406130753627444	23.685831406843423
40-41	23.91703312566788	26.02048318012575	26.38442742399601	23.678056270210355
42-43	23.65304110100375	26.05046944965437	26.32490278078316	23.97158666855872
44-45	23.67245130726942	26.162703343865	26.434927180819212	23.72991816804637
46-47	23.8882634694655	26.13219701995968	26.227201321798983	23.752338188775838
48-49	23.619609447972202	26.32335050456363	26.196107321758948	23.86093272570522
50-51	23.766354896163772	26.37346456487319	26.205201806160105	23.654978732802935
52-53	23.960987832828355	26.16736982478939	26.185218976344736	23.686423366037516
54-55	23.359083027246278	26.159945450086013	26.544714107342283	23.936257415325425
56-57	23.60855017656447	26.26154400328237	26.27796014366584	23.85194567648731
58-59	23.493693905424955	26.160119985213754	26.379002673211318	23.967183436149977
60-61	23.47572173028635	26.286469627870463	26.521217641886853	23.71659099995634
62-63	23.561647647609078	26.1965523810747	26.48169910716388	23.760100864152342
64-65	23.262763166205108	26.123386640090008	26.483498955738515	24.130351237966373
66-67	23.293208638633413	26.327283114971994	26.305655052968408	24.073853193426185
68-69	23.59821913968765	26.05524195629128	26.415752044771168	23.9307868592499
70-71	23.40190097400129	25.998238355661556	26.337546560114237	24.262314110222913
72-73	23.36170589007256	26.208338444589597	26.230389099868702	24.199566565469144
74-75	23.27475813326015	26.24568274773118	26.27173503984053	24.20782407916814
76-77	23.341792960491997	25.98965197128188	26.350709030008513	24.31784603821761
78-79	23.274427722249737	26.06097784570991	26.337006866133528	24.32758756590683
80-81	23.370777854515666	26.12398344603096	26.159447058886514	24.345791640566862
82-83	23.31014581484736	26.183114677067692	26.181487733164072	24.325251774920872
84-85	23.253493484097053	26.11219575073454	26.20720104593614	24.427109719232266
86-87	23.13300250392121	26.236327159621336	26.171651808468443	24.459018527989006
88-89	23.01561101464083	26.143137099180276	26.145841606047792	24.695410280131107
90-91	23.016356156279663	26.117324371698903	26.195329400597682	24.670990071423756
92-93	23.067068365966048	25.958532469979616	26.35567089778509	24.618728266269247
94-95	22.950361428660408	25.756712162465572	26.458893790507286	24.834032618366734
96-97	22.919364525973723	25.542721890856722	26.499117920406924	25.038795662762624
98-99	22.699555615670477	25.616261162042335	26.47466084769457	25.20952237459262
100-101	22.746157624345514	25.592902399431576	26.44883484766932	25.212105128553592
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	9.0
2	15.0
3	26.0
4	26.0
5	30.0
6	56.0
7	103.0
8	195.0
9	246.5
10	208.0
11	185.5
12	201.0
13	206.0
14	231.0
15	299.5
16	335.5
17	384.5
18	464.0
19	526.5
20	635.0
21	761.5
22	950.0
23	1209.5
24	1580.5
25	2285.0
26	3300.5
27	4843.5
28	7093.0
29	9931.5
30	13545.0
31	18309.5
32	25279.5
33	29670.5
34	31658.0
35	35745.5
36	41996.0
37	48820.0
38	54337.5
39	59227.0
40	64371.0
41	69921.0
42	74396.0
43	78480.5
44	83042.5
45	88954.5
46	93250.0
47	96030.0
48	98819.5
49	99947.5
50	100202.5
51	100041.5
52	99874.5
53	99296.0
54	97125.5
55	92161.5
56	85279.0
57	77300.5
58	68914.0
59	59387.0
60	49020.5
61	40346.0
62	32740.5
63	24821.5
64	17659.5
65	12675.5
66	9483.0
67	7456.5
68	5732.5
69	4189.0
70	3069.0
71	2151.5
72	1549.0
73	1162.5
74	865.0
75	626.0
76	425.0
77	237.0
78	124.5
79	76.0
80	44.5
81	27.5
82	18.0
83	15.0
84	13.5
85	10.5
86	7.5
87	3.5
88	2.0
89	1.0
90	2.0
91	1.5
92	1.0
93	1.5
94	0.5
95	1.0
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1644489739488342
2	1.7121184169581905E-4
3	0.011299981551924057
4	0.00556438485511412
5	0.02418367263953444
6	0.001626512496110281
7	0.0
8	0.0
9	4.280296042395476E-5
10-11	0.04804632307588922
12-13	2.9962072296768334E-4
14-15	0.004601318245575137
16-17	0.0
18-19	0.0014338991742024847
20-21	2.9962072296768334E-4
22-23	0.0
24-25	6.420444063593214E-4
26-27	0.0026109805858612403
28-29	0.005371771533206322
30-31	0.015879898317287217
32-33	0.011235777111288125
34-35	0.009630666095389822
36-37	0.003681054596460109
38-39	0.0
40-41	0.003317229432856494
42-43	0.00858199356500293
44-45	0.006762867746984853
46-47	0.003295827952644517
48-49	0.008945818728606544
50-51	0.0025681776254372858
52-53	0.0017763228575941228
54-55	0.0033386309130684715
56-57	0.0073407077127082415
58-59	0.014231984340964959
60-61	0.007062488469952536
62-63	0.020202997320106646
64-65	0.03871527770346708
66-67	0.05810501877551859
68-69	0.04808912603631318
70-71	0.0657667486914065
72-73	0.03233763660029783
74-75	0.02568177625437286
76-77	0.22321743861092408
78-79	0.08265251657865665
80-81	0.06418303915572016
82-83	0.026516433982639975
84-85	0.049394616329243794
86-87	0.06630178569670593
88-89	0.29285785522069846
90-91	0.2974805749464856
92-93	0.05166317323171339
94-95	0.009010023169242478
96-97	0.014124976939905071
98-99	8.988621689030501E-4
100-101	1.9261332190779643E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2336287.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.618292836602016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70987468491445	49.28677982152221
2	12.19240534268174	15.269352162611922
3	3.931967659410149	7.3863930696298
4	1.7392707681046764	4.356406651172814
5	0.9534808925480328	2.9852672871838695
6	0.5687885029389667	2.136993902347481
7	0.3909641974319097	1.7137057422392885
8	0.2805922934710816	1.405616832021277
9	0.2046908449774857	1.1535652144594535
>10	0.981244879998411	10.532878409307095
>50	0.034638359602563513	1.4347884303279506
>100	0.010231196227229282	1.1936533092504884
>500	0.0010965201145172955	0.5041952468494381
>1k	7.538575787303642E-4	0.6404039210768631
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.280296042395476E-5	0.0
3	0.0	0.0	0.0	4.280296042395476E-5	0.0
4	0.0	0.0	0.0	8.560592084790953E-5	0.0
5	0.0	0.0	0.0	8.560592084790953E-5	0.0
6	0.0	0.0	0.0	8.560592084790953E-5	0.0
7	0.0	0.0	0.0	8.560592084790953E-5	0.0
8	0.0	0.0	0.0	8.560592084790953E-5	0.0
9	0.0	0.0	0.0	8.560592084790953E-5	0.0
10-11	4.280296042395476E-5	0.0	0.0	8.560592084790953E-5	0.0
12-13	4.280296042395476E-5	0.0	0.0	1.284088812718643E-4	4.280296042395476E-5
14-15	4.280296042395476E-5	0.0	0.0	1.4981036148384167E-4	4.280296042395476E-5
16-17	4.280296042395476E-5	0.0	0.0	1.7121184169581905E-4	4.280296042395476E-5
18-19	4.280296042395476E-5	0.0	0.0	1.7121184169581905E-4	4.280296042395476E-5
20-21	4.280296042395476E-5	0.0	0.0	1.7121184169581905E-4	1.0700740105988691E-4
22-23	4.280296042395476E-5	0.0	0.0	1.7121184169581905E-4	1.284088812718643E-4
24-25	4.280296042395476E-5	0.0	0.0	2.1401480211977381E-4	1.284088812718643E-4
26-27	4.280296042395476E-5	0.0	0.0	3.638251636036155E-4	1.284088812718643E-4
28-29	4.280296042395476E-5	0.0	0.0	0.002760790947345082	1.284088812718643E-4
30-31	4.280296042395476E-5	0.0	0.0	0.01249846444379479	1.284088812718643E-4
32-33	4.280296042395476E-5	0.0	0.0	0.030218890059312064	1.7121184169581905E-4
34-35	4.280296042395476E-5	0.0	0.0	0.052476429479768535	1.7121184169581905E-4
36-37	4.280296042395476E-5	0.0	0.0	0.09072087461857212	1.7121184169581905E-4
38-39	4.280296042395476E-5	0.0	0.0	0.15488251229408032	1.7121184169581905E-4
40-41	4.280296042395476E-5	0.0	0.0	0.2333189372709774	1.7121184169581905E-4
42-43	4.280296042395476E-5	0.0	0.0	0.31550062128497053	1.7121184169581905E-4
44-45	4.280296042395476E-5	0.0	0.0	0.40885387796961586	1.7121184169581905E-4
46-47	4.280296042395476E-5	0.0	0.0	0.5181726388923964	1.7121184169581905E-4
48-49	4.280296042395476E-5	0.0	0.0	0.6404820983038471	1.7121184169581905E-4
50-51	4.280296042395476E-5	0.0	0.0	0.7756966502831202	1.7121184169581905E-4
52-53	4.280296042395476E-5	0.0	0.0	0.9137790006107982	2.1401480211977381E-4
54-55	4.280296042395476E-5	0.0	0.0	1.0611067903900504	2.568177625437286E-4
56-57	4.280296042395476E-5	4.280296042395476E-5	0.0	1.2308419299512432	2.568177625437286E-4
58-59	4.280296042395476E-5	8.560592084790953E-5	0.0	1.4199882120646992	2.568177625437286E-4
60-61	4.280296042395476E-5	8.560592084790953E-5	0.0	1.6300865433056813	2.568177625437286E-4
62-63	4.280296042395476E-5	1.284088812718643E-4	0.0	1.8385797635307648	2.568177625437286E-4
64-65	4.280296042395476E-5	1.7121184169581905E-4	0.0	2.0548203195925843	2.568177625437286E-4
66-67	6.420444063593214E-5	1.7121184169581905E-4	0.0	2.304233169982969	2.568177625437286E-4
68-69	8.560592084790953E-5	1.7121184169581905E-4	0.0	2.5844427503983884	2.568177625437286E-4
70-71	8.560592084790953E-5	1.7121184169581905E-4	0.0	2.8850479414558228	2.568177625437286E-4
72-73	8.560592084790953E-5	1.7121184169581905E-4	0.0	3.1986438310019274	2.9962072296768334E-4
74-75	8.560592084790953E-5	2.1401480211977381E-4	0.0	3.518125127606326	2.9962072296768334E-4
76-77	8.560592084790953E-5	2.1401480211977381E-4	0.0	3.8626889590191618	2.9962072296768334E-4
78-79	8.560592084790953E-5	2.1401480211977381E-4	0.0	4.2587447518220145	2.9962072296768334E-4
80-81	8.560592084790953E-5	2.1401480211977381E-4	0.0	4.689064314444245	2.9962072296768334E-4
82-83	8.560592084790953E-5	2.1401480211977381E-4	0.0	5.130833668979882	2.9962072296768334E-4
84-85	8.560592084790953E-5	2.1401480211977381E-4	0.0	5.572881242758275	2.9962072296768334E-4
86-87	8.560592084790953E-5	2.1401480211977381E-4	0.0	6.0401825631868	2.9962072296768334E-4
88-89	8.560592084790953E-5	2.1401480211977381E-4	0.0	6.560923379704634	2.9962072296768334E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1600	0.0	41.901405	1
GTATCAA	2700	0.0	39.97529	1
TCAACGC	3030	0.0	33.839504	4
ATCAACG	3185	0.0	32.34311	3
CAACGCA	3175	0.0	32.167297	5
AACGCAG	3315	0.0	30.943478	6
TATCAAC	3460	0.0	30.45849	2
GTACATG	5155	0.0	29.607782	1
TACATGG	5370	0.0	26.962305	2
ACATGGG	5425	0.0	25.201374	3
ACGCAGA	4120	0.0	24.666424	7
GTATAGC	770	0.0	23.4651	1
CGCAGAG	4345	0.0	22.951923	8
CATGGGG	4510	0.0	22.839998	4
GAGTACA	4010	0.0	21.580252	1
TATACCG	240	1.4138459E-7	19.792862	5
TAGACCG	150	7.721176E-4	19.001148	5
GTGTAGC	1355	0.0	18.948896	1
AGTACAT	4200	0.0	18.87551	2
TAGAGCG	280	3.4904588E-8	18.661842	5
>>END_MODULE