FastQCFastQC Report
Mon 15 Aug 2016
SRR2921672_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921672_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2491918
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20050.047.3437271
GTATCAA36050.044.1048551
TCAACGC43200.035.3796774
CAACGCA44350.034.4608965
ATCAACG44850.033.8657383
TATCAAC46150.033.1174742
AACGCAG48400.031.947096
GTACATG63650.029.6032221
TACATGG63900.028.5235392
ACGCAGA57050.026.5271157
ACATGGG67900.025.3053173
CGCAGAG58900.025.2111578
CATGGGG59800.021.5898424
GCAGAGT66600.021.3711649
ATGGGGG36350.019.5862435
GTATAGG6800.019.5432131
AGAGTAC57200.018.45578610-11
GAGTACA48950.017.9376641
GAGTACT33000.017.61319712-13
CAGAGTA63000.017.54744710-11
Produced by FastQC (version 0.11.5)