##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921672_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2491918
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.979546678502263	31.0	31.0	34.0	26.0	34.0
2	30.03446020294408	31.0	31.0	34.0	26.0	34.0
3	30.08513883683171	31.0	31.0	34.0	25.0	34.0
4	33.54312862622285	37.0	35.0	37.0	28.0	37.0
5	33.53223460804087	37.0	35.0	37.0	28.0	37.0
6	33.565027420645464	37.0	35.0	37.0	28.0	37.0
7	33.47249427950679	37.0	35.0	37.0	28.0	37.0
8	33.43594171236774	37.0	35.0	37.0	27.0	37.0
9	34.943766608692584	39.0	35.0	39.0	28.0	39.0
10-11	34.82661708772119	39.0	35.0	39.0	27.0	39.0
12-13	34.709316879608394	39.0	35.0	39.0	27.0	39.0
14-15	35.78929443103665	40.0	36.0	41.0	27.0	41.0
16-17	35.71989648134489	40.0	36.0	41.0	27.0	41.0
18-19	35.68416456721289	40.0	36.0	41.0	27.0	41.0
20-21	35.597662724054324	40.0	36.0	41.0	26.0	41.0
22-23	35.49009718618349	40.0	36.0	41.0	25.5	41.0
24-25	35.37066147441449	40.0	36.0	41.0	25.0	41.0
26-27	35.20766674505341	39.5	35.0	41.0	24.0	41.0
28-29	35.05931655857054	39.0	35.0	41.0	24.0	41.0
30-31	34.89404827927724	39.0	35.0	41.0	23.5	41.0
32-33	34.70333935546836	39.0	34.5	41.0	22.5	41.0
34-35	34.5126830016076	39.0	34.0	41.0	21.0	41.0
36-37	34.46874596194578	39.0	34.5	41.0	20.0	41.0
38-39	34.637094599421005	39.0	35.0	41.0	21.5	41.0
40-41	34.68427171359571	39.0	35.0	41.0	20.5	41.0
42-43	34.50855365224699	39.0	35.0	41.0	18.0	41.0
44-45	34.371946629062435	39.0	34.5	41.0	16.0	41.0
46-47	34.18850198923079	39.0	34.0	41.0	14.5	41.0
48-49	34.00238009436907	39.0	34.0	41.0	12.0	41.0
50-51	32.31078049117186	37.0	32.0	39.5	8.5	40.0
52-53	32.4190767914514	37.5	32.0	39.5	8.0	40.0
54-55	33.311133632808136	38.0	33.0	40.0	7.0	41.0
56-57	33.32343540196748	38.0	33.0	40.0	4.5	41.0
58-59	33.120374747483666	38.0	33.0	40.0	2.0	41.0
60-61	32.86382256559004	37.0	33.0	40.0	2.0	41.0
62-63	32.562016085601535	37.0	32.5	40.0	2.0	41.0
64-65	32.263251840550126	36.0	32.0	39.5	2.0	41.0
66-67	31.911031382252546	36.0	32.0	39.0	2.0	41.0
68-69	31.548254798111337	35.0	32.0	39.0	2.0	41.0
70-71	31.132939366383646	35.0	31.0	38.5	2.0	40.0
72-73	30.77622498011572	35.0	31.0	37.0	2.0	39.5
74-75	30.381551078326012	35.0	31.0	37.0	2.0	39.0
76-77	30.011951637252913	35.0	30.5	36.0	2.0	39.0
78-79	29.66684056216938	35.0	30.0	36.0	2.0	37.5
80-81	29.362768156897616	35.0	30.0	35.0	2.0	37.0
82-83	29.052826577760584	35.0	30.0	35.0	2.0	37.0
84-85	28.794522331794226	34.0	29.0	35.0	2.0	36.0
86-87	28.556341942230844	34.0	29.0	35.0	2.0	36.0
88-89	28.325117239010275	34.0	29.0	35.0	2.0	36.0
90-91	28.087263505460456	34.0	29.0	35.0	2.0	35.0
92-93	27.88493180754744	34.0	29.0	35.0	2.0	35.0
94-95	27.638034839027608	34.0	29.0	35.0	2.0	35.0
96-97	27.38051131698555	34.0	27.5	35.0	2.0	35.0
98-99	27.076687916697097	34.0	27.0	35.0	2.0	35.0
100-101	25.98891897726972	33.0	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101934.0
3	17171.0
4	11757.0
5	9096.0
6	8330.0
7	8667.0
8	8906.0
9	9609.0
10	10452.0
11	10985.0
12	10977.0
13	11111.0
14	11676.0
15	12291.0
16	12819.0
17	13491.0
18	12504.0
19	12101.0
20	12035.0
21	12693.0
22	14152.0
23	16128.0
24	18543.0
25	21698.0
26	25702.0
27	29931.0
28	35127.0
29	41413.0
30	48692.0
31	58661.0
32	73094.0
33	94042.0
34	128375.0
35	184574.0
36	301432.0
37	509502.0
38	491142.0
39	81097.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.517730177518594	17.63798926825978	12.386872650123106	25.457407904098524
2	16.174487372360154	19.296879455296235	38.96307410319781	25.56555906914579
3	19.29422196591868	23.080972955737174	29.447135319302053	28.177669759042097
4	13.50456141027232	15.177004886092247	35.81428913923737	35.50414456439807
5	14.271883062775453	36.94051742312441	34.36448513688522	14.42311437721491
6	34.37466513570557	34.77435514417777	16.508573930611504	14.342405789505158
7	29.331086309476028	31.59217078879688	20.680472565331723	18.396270336395364
8	27.010563503234795	34.49897511662639	19.697748708309803	18.792712671829012
9	27.129466797550663	15.084393688994266	19.051928465422627	38.73421104803245
10-11	25.917470867171964	24.697193174680578	27.789918546479075	21.595417411668382
12-13	26.34494285351317	22.263759098693264	27.50685820056953	23.884439847224037
14-15	23.42207512103297	23.932803241810817	24.50874735317219	28.136374283984022
16-17	23.789469036406093	27.099042758269114	25.52171631164395	23.58977189368084
18-19	23.900917970231486	26.306210275503446	26.800572943777585	22.992298810487487
20-21	24.169150336923558	25.625135338371518	26.808386553883185	23.39732777082174
22-23	24.113983530368618	25.40454490529741	26.545668843428505	23.935802720905464
24-25	23.967573159401056	25.621832579344456	26.49374198265856	23.91685227859593
26-27	23.677323244901682	25.833711462211827	26.48159122786491	24.007374065021576
28-29	23.869900439907354	26.14689558491304	26.230795441273912	23.752408533905694
30-31	24.07976528102444	25.83241175914616	26.036372130151758	24.05145082967764
32-33	23.763843458330715	25.9053342325691	26.031992432614004	24.298829876486185
34-35	24.250631116654596	25.861445890713732	26.1009903028457	23.78693268978597
36-37	24.35361701188448	25.623027362600098	26.17503476256117	23.848320862954246
38-39	24.068136907886394	25.75462430111773	26.145259960928406	24.03197883006747
40-41	24.213370691737783	25.676111140985707	26.215960685389213	23.8945574818873
42-43	24.06397366528461	25.69004339242877	25.9916947824506	24.25428815983602
44-45	23.918759028477968	25.761558713631004	26.246133524466487	24.073548733424545
46-47	24.21359305287751	25.774081796375643	25.939455964226905	24.072869186519945
48-49	23.939316676191126	25.914679634405637	25.867224916461602	24.278778772941642
50-51	24.02623932263204	25.905279149687026	26.09622720264822	23.97225432503271
52-53	24.28261512572194	25.65928388291167	26.035960079923797	24.022140911442587
54-55	23.634684165529286	25.604713690436455	26.398592680943604	24.36200946309065
56-57	23.76299618340948	25.80225110676299	26.186242619820653	24.24851009000687
58-59	23.88204399007964	25.56624503323377	26.193517016459328	24.358193960227258
60-61	23.687945744104073	25.65026553598692	26.428150836667612	24.23363788324139
62-63	23.787241332440043	25.633714912136075	26.47346709636842	24.105576659055465
64-65	23.58207700780518	25.545777246235247	26.38840575980131	24.483739986158266
66-67	23.561835315870542	25.63284795503537	26.244026099078138	24.56129063001595
68-69	23.785697827921805	25.419330478619607	26.42581209153152	24.369159601927073
70-71	23.621465495248327	25.31707484769732	26.30673316257018	24.75472649448417
72-73	23.56779938756266	25.462514853515682	26.26360943949718	24.70607631942448
74-75	23.51422461492929	25.673330877716726	26.28553942068895	24.52690508666504
76-77	23.602948006187454	25.24677629676928	26.411949646336563	24.738326050706704
78-79	23.532530950018682	25.298179093661034	26.43310360026849	24.7361863560518
80-81	23.709182951119292	25.401574119062865	26.31219491789933	24.577048011918514
82-83	23.61427155352456	25.39370090616518	26.374838536725942	24.61718900358432
84-85	23.494099347343674	25.453817853483635	26.46044295365446	24.59163984551823
86-87	23.57722301738058	25.525709628549752	26.248009757518272	24.649057596551398
88-89	23.380418507319202	25.287021124473195	26.361576728185437	24.970983640022162
90-91	23.331843608678966	25.204894127642184	26.45618238413335	25.0070798795455
92-93	23.524406152291622	25.040375570235796	26.628085789689838	24.807132487782745
94-95	23.392534937984237	24.786775870138484	26.815344132331404	25.005345059545874
96-97	23.443562293613613	24.531570667643425	26.866893516945684	25.157973521797278
98-99	23.214418603715675	24.619995132655255	26.803220492728148	25.36236577090092
100-101	23.372255412137484	24.410763066781694	26.960849488399713	25.256132032681112
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	39.0
2	57.5
3	90.5
4	95.0
5	113.0
6	175.0
7	305.5
8	463.0
9	548.5
10	500.0
11	445.5
12	504.0
13	593.5
14	651.5
15	722.5
16	842.5
17	943.0
18	1052.5
19	1196.5
20	1257.0
21	1369.0
22	1571.5
23	1802.0
24	2171.0
25	2689.5
26	3488.5
27	4706.0
28	6467.0
29	9088.5
30	12457.5
31	17090.5
32	24361.5
33	28454.5
34	29964.0
35	34176.0
36	40458.5
37	46968.5
38	53001.5
39	58752.0
40	63780.0
41	68991.0
42	74130.0
43	80489.5
44	86760.0
45	93415.0
46	98455.5
47	102070.5
48	105781.5
49	107506.5
50	107626.0
51	107153.5
52	107020.5
53	106723.0
54	105719.0
55	101510.5
56	93431.5
57	84287.5
58	76924.0
59	68109.0
60	56335.5
61	46574.5
62	39324.5
63	30601.0
64	22060.5
65	16361.0
66	12363.5
67	9682.5
68	7416.5
69	5539.5
70	4220.5
71	3092.0
72	2192.5
73	1653.0
74	1326.5
75	1005.5
76	711.5
77	485.5
78	326.0
79	218.5
80	158.0
81	116.0
82	104.0
83	87.5
84	59.0
85	45.0
86	45.0
87	41.0
88	32.0
89	24.0
90	24.5
91	26.5
92	24.0
93	22.0
94	18.0
95	16.0
96	14.0
97	11.0
98	9.5
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.054656694160883305
2	0.0764070085773288
3	0.07283546248311541
4	0.07179208946682836
5	0.0679396352528454
6	0.009510746340770444
7	0.013082292434983815
8	0.01665383852919719
9	0.005778681320974447
10-11	0.012560605926840291
12-13	0.02237232525307815
14-15	0.03870512593111009
16-17	0.02590374161589587
18-19	0.009029189564022573
20-21	0.01916194674142568
22-23	0.03647792583865119
24-25	0.03344813111827918
26-27	0.019402725129799615
28-29	0.010313340968683561
30-31	0.010333405834381387
32-33	0.0069223786657506385
34-35	0.004193556930846039
36-37	5.216865081435263E-4
38-39	0.003290637974443782
40-41	8.025946279131175E-4
42-43	0.2315485501529344
44-45	0.2004680731869989
46-47	0.20598591125390162
48-49	0.21417237645861542
50-51	0.20530370582017549
52-53	0.21266751153127833
54-55	0.20883512218299316
56-57	0.2116642682463869
58-59	0.22137566324413566
60-61	0.22576986883195996
62-63	0.20995875466207153
64-65	0.2100791438562585
66-67	0.2072098680614691
68-69	0.20213345703991864
70-71	0.19452887294044185
72-73	0.20598591125390162
74-75	0.20474188958063627
76-77	0.19860204067710094
78-79	0.21706171711910263
80-81	0.22881972841802983
82-83	0.2275957716104623
84-85	0.2223387767976314
86-87	0.24238357762976148
88-89	0.23357510158841505
90-91	0.24077838837393528
92-93	0.23616346926343484
94-95	0.239875469417533
96-97	0.24629622644083793
98-99	0.239293588312296
100-101	0.24637648590362923
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2491918.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.1577757992158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.83237952114615	46.63548233430655
2	11.398155991344748	13.485791133209215
3	3.825356670898595	6.788987768671609
4	1.8438574478487457	4.363140220222013
5	1.0434123299645464	3.086297634109
6	0.6740682764703831	2.3925827983679238
7	0.4689198523099939	1.94181788435292
8	0.3386593960888263	1.6027469300896484
9	0.25209161074926906	1.342186109061159
>10	1.2526329892200363	12.838836892532616
>50	0.050659370274698544	2.0048322820791187
>100	0.017148500515805198	1.8418035476497725
>500	0.0013630990606540755	0.5382177210990595
>1k	0.0012949441076155629	1.1372767442494423
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.025946279131175E-5
2	0.0	0.0	0.0	0.0	8.025946279131175E-5
3	0.0	0.0	0.0	0.0	8.025946279131175E-5
4	0.0	0.0	0.0	0.0	8.025946279131175E-5
5	0.0	0.0	0.0	0.0	8.025946279131175E-5
6	0.0	0.0	0.0	0.0	8.025946279131175E-5
7	0.0	0.0	0.0	0.0	8.025946279131175E-5
8	0.0	0.0	0.0	0.0	8.025946279131175E-5
9	0.0	0.0	0.0	0.0	8.025946279131175E-5
10-11	0.0	0.0	0.0	2.0064865697827937E-5	8.025946279131175E-5
12-13	0.0	0.0	0.0	4.0129731395655874E-5	2.6084325407176316E-4
14-15	0.0	0.0	0.0	4.0129731395655874E-5	3.6116758256090286E-4
16-17	0.0	0.0	0.0	4.0129731395655874E-5	4.012973139565588E-4
18-19	0.0	0.0	0.0	4.0129731395655874E-5	4.012973139565588E-4
20-21	0.0	0.0	0.0	6.0194597093483814E-5	4.6149191105004255E-4
22-23	0.0	0.0	0.0	1.003243284891397E-4	5.016216424456985E-4
24-25	0.0	0.0	0.0	3.4110271686307495E-4	5.216865081435264E-4
26-27	0.0	0.0	0.0	8.627892250066013E-4	5.618162395391822E-4
28-29	0.0	0.0	0.0	0.0037922596168894806	5.618162395391822E-4
30-31	0.0	0.0	0.0	0.016974876380362436	5.618162395391822E-4
32-33	0.0	0.0	0.0	0.050282553438756816	5.618162395391822E-4
34-35	0.0	0.0	0.0	0.09576960397573275	5.618162395391822E-4
36-37	0.0	0.0	0.0	0.1690063637728047	5.618162395391822E-4
38-39	0.0	0.0	0.0	0.28237285496553255	6.019459709348382E-4
40-41	0.0	0.0	0.0	0.4062533357839223	6.019459709348382E-4
42-43	0.0	0.0	0.0	0.5385008655983061	6.019459709348382E-4
44-45	0.0	0.0	0.0	0.696993239745449	6.019459709348382E-4
46-47	0.0	0.0	0.0	0.8813893555084878	6.22010836632666E-4
48-49	0.0	0.0	0.0	1.074955114895434	6.822054337261499E-4
50-51	0.0	0.0	0.0	1.2816232315830618	7.624648965174616E-4
52-53	0.0	0.0	0.0	1.485201358953224	7.825297622152896E-4
54-55	0.0	0.0	0.0	1.7208431417085155	8.226594936109454E-4
56-57	0.0	0.0	0.0	1.9974172504873755	8.627892250066013E-4
58-59	0.0	0.0	0.0	2.2802114676325624	8.828540907044293E-4
60-61	0.0	0.0	0.0	2.5811443233685862	8.828540907044293E-4
62-63	0.0	0.0	0.0	2.892591168730271	9.229838221000851E-4
64-65	0.0	0.0	0.0	3.221253668860693	9.229838221000851E-4
66-67	0.0	0.0	0.0	3.6046531226147893	9.83178419193569E-4
68-69	0.0	0.0	0.0	4.012892880102796	0.001003243284891397
70-71	0.0	0.0	0.0	4.437445373403138	0.001003243284891397
72-73	0.0	0.0	0.0	4.885112591987377	0.0010634378819848807
74-75	0.0	0.0	0.0	5.3541689574055	0.0011035676133805366
76-77	0.0	0.0	0.0	5.876517606117055	0.0011436973447761923
78-79	0.0	0.0	0.0	6.4619903223139765	0.0012239568075675042
80-81	0.0	0.0	0.0	7.053743341474318	0.0012440216732653323
82-83	0.0	0.0	0.0	7.669935367054614	0.0012440216732653323
84-85	0.0	0.0	0.0	8.307456344871701	0.0012440216732653323
86-87	0.0	0.0	0.0	8.994116178782768	0.0012440216732653323
88-89	0.0	0.0	0.0	9.728028771412221	0.0012440216732653323
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2005	0.0	47.343727	1
GTATCAA	3605	0.0	44.104855	1
TCAACGC	4320	0.0	35.379677	4
CAACGCA	4435	0.0	34.460896	5
ATCAACG	4485	0.0	33.865738	3
TATCAAC	4615	0.0	33.117474	2
AACGCAG	4840	0.0	31.94709	6
GTACATG	6365	0.0	29.603222	1
TACATGG	6390	0.0	28.523539	2
ACGCAGA	5705	0.0	26.527115	7
ACATGGG	6790	0.0	25.305317	3
CGCAGAG	5890	0.0	25.211157	8
CATGGGG	5980	0.0	21.589842	4
GCAGAGT	6660	0.0	21.371164	9
ATGGGGG	3635	0.0	19.586243	5
GTATAGG	680	0.0	19.543213	1
AGAGTAC	5720	0.0	18.455786	10-11
GAGTACA	4895	0.0	17.937664	1
GAGTACT	3300	0.0	17.613197	12-13
CAGAGTA	6300	0.0	17.547447	10-11
>>END_MODULE