FastQCFastQC Report
Mon 15 Aug 2016
SRR2921672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2491918
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20250.050.0090751
GTATCAA37600.044.6355741
TCAACGC45000.036.7052464
CAACGCA45400.036.1989175
ATCAACG45950.035.7397883
TATCAAC47700.034.9261052
AACGCAG48650.033.7766276
ACGCAGA60000.026.8331367
GTACATG61750.026.0239981
CGCAGAG63550.025.2594648
TACATGG65550.023.8947582
ACATGGG67550.022.6954363
GCAGAGT71550.021.1744829
TAAGGTA7000.020.3415784
GTACCGT2851.9717845E-919.996756
CATGGGG57600.019.7765334
TAGATCG1507.764355E-418.9854724
AGAGTAC60000.018.6806710-11
GAGTACT33200.017.93752112-13
CAGAGTA66450.017.47493610-11
Produced by FastQC (version 0.11.5)