##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921672_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2491918
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.367181424107855	33.0	31.0	34.0	28.0	34.0
2	31.498101061110358	34.0	31.0	34.0	28.0	34.0
3	31.565907064357656	34.0	31.0	34.0	28.0	34.0
4	35.06204538030545	37.0	35.0	37.0	32.0	37.0
5	34.80522954607655	37.0	35.0	37.0	32.0	37.0
6	34.781028107666465	37.0	35.0	37.0	32.0	37.0
7	34.65840930560315	37.0	35.0	37.0	32.0	37.0
8	34.60077859704854	37.0	35.0	37.0	32.0	37.0
9	36.18451971533574	39.0	37.0	39.0	32.0	39.0
10-11	36.07132738717726	39.0	37.0	39.0	31.0	39.0
12-13	35.99000448650397	39.0	37.0	39.0	31.0	39.0
14-15	37.219770473988305	40.0	38.0	41.0	31.0	41.0
16-17	37.18498040465216	40.0	38.0	41.0	31.0	41.0
18-19	37.14005557165204	40.0	38.0	41.0	31.0	41.0
20-21	37.078414899687715	40.0	37.5	41.0	31.0	41.0
22-23	36.99169535273633	40.0	37.0	41.0	30.5	41.0
24-25	36.89653953300229	40.0	37.0	41.0	30.0	41.0
26-27	36.76364370737721	40.0	37.0	41.0	30.0	41.0
28-29	36.63830310628199	40.0	37.0	41.0	30.0	41.0
30-31	36.47125808313115	40.0	36.5	41.0	30.0	41.0
32-33	36.41228282792612	40.0	36.5	41.0	30.0	41.0
34-35	36.53163567179979	40.0	37.0	41.0	30.0	41.0
36-37	36.520224381380125	40.0	37.0	41.0	30.0	41.0
38-39	36.3993331642534	40.0	37.0	41.0	30.0	41.0
40-41	36.301910215344165	40.0	36.0	41.0	29.0	41.0
42-43	36.16529897853782	40.0	36.0	41.0	28.5	41.0
44-45	36.0215033159197	40.0	36.0	41.0	28.0	41.0
46-47	35.847457861775545	40.0	35.0	41.0	27.5	41.0
48-49	35.680923690105374	40.0	35.0	41.0	27.0	41.0
50-51	35.511935786009005	39.0	35.0	41.0	27.0	41.0
52-53	35.323432191588964	39.0	35.0	41.0	26.0	41.0
54-55	35.09610709501677	39.0	35.0	41.0	26.0	41.0
56-57	34.858738329270864	39.0	35.0	41.0	26.0	41.0
58-59	34.60340047304928	38.0	34.5	40.0	25.0	41.0
60-61	34.31545038801437	38.0	34.0	40.0	24.5	41.0
62-63	34.00546205774026	37.0	34.0	40.0	23.5	41.0
64-65	33.66149989686659	36.5	34.0	39.5	23.0	41.0
66-67	33.305346524243575	36.0	33.5	39.0	22.0	41.0
68-69	32.93835049949477	35.5	33.0	39.0	22.0	40.5
70-71	32.535992155440105	35.0	33.0	38.5	20.5	40.0
72-73	32.18201441620471	35.0	33.0	37.0	20.0	39.5
74-75	31.793667769164152	35.0	32.5	37.0	18.5	39.0
76-77	29.10309990136112	33.0	28.5	34.5	11.0	36.5
78-79	30.67364816980334	34.5	31.0	35.5	12.5	37.0
80-81	30.8670706660492	35.0	32.0	35.0	13.5	37.0
82-83	30.705798304759625	35.0	32.0	35.0	11.5	37.0
84-85	30.515790046060907	35.0	32.0	35.0	8.5	36.0
86-87	30.313064675482902	35.0	32.0	35.0	6.5	36.0
88-89	30.044099163776657	34.5	31.0	35.0	2.0	35.5
90-91	29.84751023107502	34.0	31.0	35.0	2.0	35.0
92-93	29.688507406744524	34.0	31.0	35.0	2.0	35.0
94-95	29.582765965814282	34.0	31.0	35.0	2.0	35.0
96-97	29.378984782003258	34.0	31.0	35.0	2.0	35.0
98-99	29.167380106407997	34.0	31.0	35.0	2.0	35.0
100-101	28.04047564968029	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43752.0
3	15849.0
4	8833.0
5	6414.0
6	5632.0
7	5673.0
8	6149.0
9	6555.0
10	7175.0
11	7667.0
12	8492.0
13	8981.0
14	9731.0
15	10454.0
16	11506.0
17	12222.0
18	11322.0
19	10137.0
20	9774.0
21	9543.0
22	10297.0
23	11489.0
24	13646.0
25	16390.0
26	19602.0
27	23274.0
28	28290.0
29	34314.0
30	41480.0
31	51557.0
32	64887.0
33	85368.0
34	121192.0
35	182958.0
36	318532.0
37	596146.0
38	586918.0
39	69714.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52774657019134	19.102057583724875	12.897913475286204	26.47228237079758
2	15.346837812219844	20.65869849441032	37.36838430218699	26.626079391182845
3	17.98363252609015	24.17258610663059	29.297585655122372	28.54619571215689
4	13.221106671113352	16.338081415298447	34.88682420297759	35.553987710610606
5	14.071740915779646	37.314002580181096	34.287226979670834	14.32702952436842
6	34.33541998830596	35.31071281091647	16.295532626741316	14.058334574036255
7	29.209949926121165	31.814770791013185	20.638720856785817	18.33655842607983
8	26.917418630950134	35.072943812757885	19.41311873023109	18.59651882606089
9	26.888977441865038	15.155400440704886	18.868605976844332	39.08701614058574
10-11	25.90928910234225	24.990845880766795	27.70829435471891	21.391570662172047
12-13	26.366777619019622	22.418301138825008	27.461439726667553	23.753481515487813
14-15	23.319586267702782	24.10514147478263	24.500477465226506	28.074794792288078
16-17	23.756886012478343	27.37797298666589	25.396406582481163	23.468734418374602
18-19	23.8666158082367	26.487423474081563	26.741889521765554	22.904071195916185
20-21	24.106132982652074	25.76307384913148	26.750408270919735	23.380384897296715
22-23	24.077678318467942	25.53743742771632	26.517505792726727	23.867378461089007
24-25	23.906473943925217	25.764490573374704	26.46126687439266	23.867768608307422
26-27	23.665759378295107	25.982304365317837	26.445733754839512	23.90620250154754
28-29	23.856615384491903	26.272076648159974	26.190448958313894	23.68085900903423
30-31	24.01131921969549	25.950621599834157	26.07183301562162	23.96622616484873
32-33	23.752287891098693	26.05628277320595	25.99154656722522	24.19988276847014
34-35	24.18880097861605	26.048167462782168	26.030508708575173	23.73252285002661
36-37	24.292443175935283	25.763961515586637	26.128073764735248	23.815521543742832
38-39	24.086185821523824	25.840758002470388	26.11915801402775	23.953898161978042
40-41	24.139086528071875	25.778675194218582	26.182673116239798	23.899565161469745
42-43	24.062155982725038	25.768782665964068	25.952532789875505	24.21652856143539
44-45	23.947869653264327	25.846070679529358	26.17760420972536	24.028455457480955
46-47	24.148286882152203	25.84146970588666	25.909712299578768	24.10053111238237
48-49	23.894004375682634	26.02814796413168	25.85330928306509	24.224538377120595
50-51	23.97627482199595	26.071350101881418	25.962676586937306	23.989698489185322
52-53	24.28883328146473	25.781344839535393	25.919714624357937	24.010107254641937
54-55	23.61219577384187	25.759105615427153	26.26399883779447	24.364699772936504
56-57	23.69057315410842	25.94878376645619	26.016347280071933	24.344295799363454
58-59	23.74779954839067	25.723469958668034	26.06789632411197	24.460834168829322
60-61	23.612734254351185	25.84439121961556	26.24100225706094	24.301872268972318
62-63	23.702033958444215	25.852332706376096	26.24197338201283	24.203659953166856
64-65	23.373241974027433	25.696862275545264	26.22066945749474	24.709226292932566
66-67	23.4358878709572	25.845221156373167	26.040571418590236	24.678319554079398
68-69	23.6715356982686	25.632498326734627	26.118076525742996	24.57788944925379
70-71	23.515142923918745	25.565736328641087	26.04077734934379	24.878343398096384
72-73	23.432411861804965	25.72402099002524	25.96138715292274	24.882179995247057
74-75	23.369371712722174	25.87385443272468	25.894546997410515	24.862226857142627
76-77	23.378211595842647	25.493671278106394	26.133089484964174	24.995027641086786
78-79	23.36708030029673	25.50337295626483	26.091632112407243	25.0379146310312
80-81	23.468503488011617	25.586990672399974	25.94824041338921	24.996265426199198
82-83	23.35485349703831	25.66082330030212	25.958708340600495	25.025614862059076
84-85	23.228258488895243	25.664658399519958	26.02266141202105	25.08442169956374
86-87	23.2588061107584	25.794213468549703	25.801923596646255	25.145056824045643
88-89	23.074433878388348	25.60061434905926	25.818357300486483	25.506594472065913
90-91	22.910650934731038	25.51800030429172	25.938735933551705	25.63261282742554
92-93	23.007307294426006	25.34952010434461	26.12738690597241	25.515785695256977
94-95	22.843792767158032	25.079022671359603	26.308128218197286	25.76905634328508
96-97	22.772475521758647	24.938236226091433	26.305919268310745	25.98336898383917
98-99	22.60563774516679	25.007238451133983	26.187522334860482	26.199601468838747
100-101	22.52493718175786	24.95116700573034	26.249688241336017	26.274207571175783
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	39.0
2	54.5
3	84.5
4	77.5
5	85.5
6	150.0
7	273.0
8	426.0
9	476.5
10	385.5
11	304.5
12	300.5
13	334.5
14	367.0
15	417.0
16	483.0
17	572.0
18	650.0
19	695.5
20	781.0
21	931.0
22	1097.0
23	1303.5
24	1680.0
25	2186.0
26	3004.0
27	4322.5
28	6185.5
29	8838.0
30	12436.0
31	17265.5
32	24819.0
33	29042.0
34	30055.0
35	34140.0
36	40506.5
37	46985.0
38	52879.0
39	58594.5
40	63769.5
41	69318.0
42	74562.0
43	80526.5
44	87097.5
45	94366.5
46	99110.0
47	102584.5
48	106571.5
49	108213.5
50	108548.0
51	108141.0
52	108206.0
53	108653.0
54	107774.5
55	102877.5
56	94817.0
57	85412.0
58	77094.0
59	68028.0
60	56182.0
61	46414.5
62	38821.0
63	29605.5
64	21002.5
65	15445.0
66	11493.0
67	8906.5
68	6842.5
69	5052.5
70	3875.0
71	2820.5
72	1907.5
73	1384.0
74	1079.0
75	795.5
76	534.0
77	337.0
78	191.0
79	112.5
80	68.0
81	33.5
82	21.0
83	21.0
84	12.5
85	7.5
86	7.5
87	4.0
88	2.5
89	2.5
90	3.0
91	3.0
92	2.0
93	2.5
94	1.5
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16441150952800213
2	1.6051892558262352E-4
3	0.010794897745431431
4	0.007022702994239778
5	0.02488043346530664
6	0.0020466163011784496
7	0.0
8	0.0
9	4.012973139565588E-5
10-11	0.049600348005030666
12-13	3.009729854674191E-4
14-15	0.0045547245134069415
16-17	2.006486569782794E-5
18-19	0.001344346001754472
20-21	2.6084325407176316E-4
22-23	0.0
24-25	5.417513738413543E-4
26-27	0.0024278487494371805
28-29	0.0046751137075939095
30-31	0.016192346618147144
32-33	0.010734703148337948
34-35	0.009470616609374788
36-37	0.004173492065148211
38-39	0.0
40-41	0.002949535257580707
42-43	0.008788411175648636
44-45	0.006581275948887564
46-47	0.002909405526185051
48-49	0.007724973293663756
50-51	0.0020265514354806217
52-53	0.0017857730471066867
54-55	0.004173492065148211
56-57	0.007564454368081133
58-59	0.015409816855931858
60-61	0.0073036111140093694
62-63	0.020867460325741057
64-65	0.040952390889266825
66-67	0.0609771268556991
68-69	0.05132592645504387
70-71	0.06870210014936286
72-73	0.03330767705839438
74-75	0.02748886600602428
76-77	0.22767603107325363
78-79	0.08483425217041651
80-81	0.06717717035632793
82-83	0.027629320065909072
84-85	0.05202819675446784
86-87	0.06769885686447147
88-89	0.29443183924992716
90-91	0.2993677962115928
92-93	0.05315182923354621
94-95	0.00914957875820954
96-97	0.014787806019299191
98-99	7.022702994239779E-4
100-101	2.6084325407176316E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2491918.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.71800821985522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.61418817344527	44.79736350972304
2	12.067849157474617	13.930644337341855
3	4.04717938830252	7.007853996038202
4	1.9072737940841638	4.403361780978568
5	1.1330500366492655	3.2698695664414785
6	0.7040627784564994	2.4382260740547808
7	0.4784662460081005	1.9331283104013168
8	0.3648764448055393	1.6847953312414135
9	0.2580746174168466	1.3405997600461388
>10	1.3482453337097804	13.46295916298633
>50	0.055767586593119305	2.1122394210828532
>100	0.01831624887503315	1.9470227475020498
>500	0.0012553551374791224	0.48723036885119286
>1k	0.0013948390416353177	1.1847056333107813
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.025946279131175E-5
2	0.0	0.0	0.0	4.0129731395655874E-5	8.025946279131175E-5
3	0.0	0.0	0.0	4.0129731395655874E-5	8.025946279131175E-5
4	0.0	0.0	0.0	4.0129731395655874E-5	8.025946279131175E-5
5	0.0	0.0	0.0	4.0129731395655874E-5	8.025946279131175E-5
6	0.0	0.0	0.0	4.0129731395655874E-5	8.025946279131175E-5
7	0.0	0.0	0.0	4.0129731395655874E-5	8.025946279131175E-5
8	0.0	0.0	0.0	4.0129731395655874E-5	8.025946279131175E-5
9	0.0	0.0	0.0	4.0129731395655874E-5	8.025946279131175E-5
10-11	0.0	0.0	0.0	1.003243284891397E-4	8.025946279131175E-5
12-13	0.0	0.0	0.0	1.2038919418696763E-4	8.025946279131175E-5
14-15	0.0	0.0	0.0	1.2038919418696763E-4	8.025946279131175E-5
16-17	0.0	0.0	0.0	1.2038919418696763E-4	8.025946279131175E-5
18-19	0.0	0.0	0.0	1.2038919418696763E-4	8.025946279131175E-5
20-21	0.0	0.0	0.0	1.2038919418696763E-4	1.2038919418696763E-4
22-23	2.0064865697827937E-5	0.0	0.0	1.4045405988479556E-4	2.2071352267610732E-4
24-25	4.0129731395655874E-5	0.0	0.0	3.6116758256090286E-4	2.4077838837393526E-4
26-27	4.0129731395655874E-5	0.0	0.0	8.627892250066013E-4	2.6084325407176316E-4
28-29	4.0129731395655874E-5	0.0	0.0	0.0036718704227025126	2.809081197695911E-4
30-31	4.0129731395655874E-5	0.0	0.0	0.01701500611175809	3.009729854674191E-4
32-33	4.0129731395655874E-5	4.0129731395655874E-5	0.0	0.05196800215737436	3.21037851165247E-4
34-35	4.0129731395655874E-5	4.0129731395655874E-5	0.0	0.0986790095019178	3.21037851165247E-4
36-37	4.0129731395655874E-5	4.0129731395655874E-5	0.0	0.17348082882342036	3.21037851165247E-4
38-39	4.0129731395655874E-5	4.0129731395655874E-5	0.0	0.2885728984661614	3.21037851165247E-4
40-41	4.0129731395655874E-5	4.0129731395655874E-5	0.0	0.4150216820938731	3.21037851165247E-4
42-43	4.0129731395655874E-5	4.0129731395655874E-5	0.0	0.5501986823001399	3.21037851165247E-4
44-45	4.0129731395655874E-5	4.0129731395655874E-5	0.0	0.7111991646595113	3.4110271686307495E-4
46-47	4.0129731395655874E-5	4.0129731395655874E-5	0.0	0.8984645562173394	3.611675825609029E-4
48-49	4.0129731395655874E-5	4.0129731395655874E-5	0.0	1.0955416671014055	3.611675825609029E-4
50-51	4.0129731395655874E-5	4.0129731395655874E-5	0.0	1.3051793839123116	4.012973139565588E-4
52-53	4.0129731395655874E-5	4.0129731395655874E-5	0.0	1.5107840627179545	4.012973139565588E-4
54-55	4.0129731395655874E-5	4.0129731395655874E-5	0.0	1.748753369894194	4.012973139565588E-4
56-57	4.0129731395655874E-5	4.0129731395655874E-5	0.0	2.0272938355114416	4.012973139565588E-4
58-59	4.0129731395655874E-5	4.0129731395655874E-5	0.0	2.313037587914209	4.4142704535221465E-4
60-61	4.0129731395655874E-5	4.0129731395655874E-5	0.0	2.616819654579324	4.4142704535221465E-4
62-63	4.0129731395655874E-5	4.0129731395655874E-5	0.0	2.9317377217067335	5.216865081435264E-4
64-65	4.0129731395655874E-5	4.0129731395655874E-5	0.0	3.2639517030656706	5.216865081435264E-4
66-67	4.0129731395655874E-5	4.0129731395655874E-5	0.0	3.651986140795965	5.216865081435264E-4
68-69	4.0129731395655874E-5	4.0129731395655874E-5	0.0	4.065222049842731	5.216865081435264E-4
70-71	4.0129731395655874E-5	4.0129731395655874E-5	0.0	4.496295624494866	5.216865081435264E-4
72-73	4.0129731395655874E-5	4.0129731395655874E-5	0.0	4.9516276217756765	5.618162395391822E-4
74-75	4.0129731395655874E-5	4.0129731395655874E-5	0.0	5.428328701024673	5.618162395391822E-4
76-77	4.0129731395655874E-5	4.0129731395655874E-5	0.0	5.958803620343847	5.618162395391822E-4
78-79	4.0129731395655874E-5	4.0129731395655874E-5	0.0	6.551620077386174	6.019459709348382E-4
80-81	6.0194597093483814E-5	4.0129731395655874E-5	0.0	7.155191302442536	6.42075702330494E-4
82-83	8.025946279131175E-5	4.0129731395655874E-5	0.0	7.781415760871746	6.621405680283219E-4
84-85	8.025946279131175E-5	4.0129731395655874E-5	0.0	8.434246231216274	6.822054337261499E-4
86-87	8.025946279131175E-5	4.0129731395655874E-5	0.0	9.135051795444312	6.822054337261499E-4
88-89	8.025946279131175E-5	4.0129731395655874E-5	0.0	9.88443439952679	6.822054337261499E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2025	0.0	50.009075	1
GTATCAA	3760	0.0	44.635574	1
TCAACGC	4500	0.0	36.705246	4
CAACGCA	4540	0.0	36.198917	5
ATCAACG	4595	0.0	35.739788	3
TATCAAC	4770	0.0	34.926105	2
AACGCAG	4865	0.0	33.776627	6
ACGCAGA	6000	0.0	26.833136	7
GTACATG	6175	0.0	26.023998	1
CGCAGAG	6355	0.0	25.259464	8
TACATGG	6555	0.0	23.894758	2
ACATGGG	6755	0.0	22.695436	3
GCAGAGT	7155	0.0	21.174482	9
TAAGGTA	700	0.0	20.341578	4
GTACCGT	285	1.9717845E-9	19.99675	6
CATGGGG	5760	0.0	19.776533	4
TAGATCG	150	7.764355E-4	18.985472	4
AGAGTAC	6000	0.0	18.68067	10-11
GAGTACT	3320	0.0	17.937521	12-13
CAGAGTA	6645	0.0	17.474936	10-11
>>END_MODULE