FastQCFastQC Report
Mon 15 Aug 2016
SRR2921671_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921671_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278825
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24100.046.6688421
GTATCAA43200.038.997821
TCAACGC47350.034.6815344
CAACGCA49650.033.26465
ATCAACG51050.032.0744253
AACGCAG53600.030.974686
TATCAAC53750.030.1100482
GTACATG84250.028.8963661
TACATGG88300.026.6597772
ACATGGG89150.025.9264563
ACGCAGA65650.024.5720027
CGCAGAG67800.023.304378
CATGGGG77050.023.2841974
ATGGGGG46550.018.8614185
GCAGAGT80200.018.6954359
GAGTACA63650.018.4159581
TACCGTG3951.8189894E-1118.0173347
GTATATA12400.017.9875451
GTATAGC9150.017.1154611
AGAGTAC66600.017.02771810-11
Produced by FastQC (version 0.11.5)