##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921671_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3278825
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.8902067051459	31.0	31.0	34.0	26.0	34.0
2	29.950419128803762	31.0	31.0	34.0	25.0	34.0
3	30.00764542175932	31.0	31.0	34.0	25.0	34.0
4	33.45704452052183	37.0	35.0	37.0	28.0	37.0
5	33.44856343354708	37.0	35.0	37.0	28.0	37.0
6	33.48218187917928	37.0	35.0	37.0	28.0	37.0
7	33.381040769177986	37.0	35.0	37.0	27.0	37.0
8	33.34620877905957	37.0	35.0	37.0	27.0	37.0
9	34.844951468895104	39.0	35.0	39.0	27.0	39.0
10-11	34.72813721378848	39.0	35.0	39.0	27.0	39.0
12-13	34.61253970553476	39.0	35.0	39.0	27.0	39.0
14-15	35.68650705664377	40.0	36.0	41.0	27.0	41.0
16-17	35.61982813965369	40.0	36.0	41.0	26.5	41.0
18-19	35.588199431198674	40.0	36.0	41.0	26.0	41.0
20-21	35.50963287153172	40.0	36.0	41.0	25.5	41.0
22-23	35.40395644781286	40.0	36.0	41.0	25.0	41.0
24-25	35.2902131098793	40.0	35.5	41.0	25.0	41.0
26-27	35.13988029248283	39.0	35.0	41.0	24.0	41.0
28-29	34.997225073006334	39.0	35.0	41.0	23.5	41.0
30-31	34.84693281892142	39.0	35.0	41.0	23.0	41.0
32-33	34.664979070246204	39.0	34.5	41.0	22.0	41.0
34-35	34.4887946139242	39.0	34.0	41.0	20.0	41.0
36-37	34.45327655486341	39.0	34.5	41.0	20.0	41.0
38-39	34.628401790275475	39.0	35.0	41.0	20.5	41.0
40-41	34.68727562465213	39.0	35.0	41.0	20.0	41.0
42-43	34.52760318101759	39.0	35.0	41.0	18.0	41.0
44-45	34.406109048210865	39.0	34.5	41.0	15.5	41.0
46-47	34.250305978513644	39.0	34.0	41.0	15.0	41.0
48-49	34.086340228587986	39.0	34.0	41.0	12.5	41.0
50-51	32.43865165112502	37.0	32.0	39.5	8.5	40.0
52-53	32.55126028379068	37.5	32.0	39.5	8.0	40.0
54-55	33.45776810290272	38.0	33.0	40.0	8.0	41.0
56-57	33.48789246147629	38.0	33.0	41.0	7.0	41.0
58-59	33.309457808818706	38.0	33.0	40.0	2.0	41.0
60-61	33.0642978811007	38.0	33.0	40.0	2.0	41.0
62-63	32.779306001387695	37.0	33.0	40.0	2.0	41.0
64-65	32.503234085381195	37.0	32.5	40.0	2.0	41.0
66-67	32.167477831235274	36.0	32.0	39.5	2.0	41.0
68-69	31.814436574077604	36.0	32.0	39.0	2.0	41.0
70-71	31.408217272956016	35.0	31.5	39.0	2.0	40.5
72-73	31.0488446318422	35.0	31.0	37.5	2.0	40.0
74-75	30.65027227741645	35.0	31.0	37.0	2.0	39.0
76-77	30.272131022546183	35.0	31.0	36.5	2.0	39.0
78-79	29.92194612399259	35.0	30.5	36.0	2.0	38.5
80-81	29.600561176641023	35.0	30.0	36.0	2.0	37.0
82-83	29.281852340396334	35.0	30.0	35.0	2.0	37.0
84-85	29.01212568526835	34.5	30.0	35.0	2.0	36.5
86-87	28.768700830327937	34.0	29.5	35.0	2.0	36.0
88-89	28.544461964270738	34.0	29.0	35.0	2.0	36.0
90-91	28.31088728431679	34.0	29.0	35.0	2.0	35.5
92-93	28.113958963958126	34.0	29.0	35.0	2.0	35.0
94-95	27.876699884867293	34.0	29.0	35.0	2.0	35.0
96-97	27.630759952116993	34.0	29.0	35.0	2.0	35.0
98-99	27.34860262441576	34.0	28.5	35.0	2.0	35.0
100-101	26.282829367227592	33.0	25.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	140615.0
3	23242.0
4	15454.0
5	11482.0
6	10438.0
7	10680.0
8	11375.0
9	12170.0
10	13096.0
11	14191.0
12	14027.0
13	13992.0
14	14862.0
15	15373.0
16	15763.0
17	16314.0
18	15370.0
19	14906.0
20	15236.0
21	15798.0
22	17652.0
23	20199.0
24	23661.0
25	27769.0
26	32483.0
27	38579.0
28	45204.0
29	52581.0
30	62794.0
31	75495.0
32	93617.0
33	120092.0
34	163149.0
35	232899.0
36	376817.0
37	659631.0
38	697273.0
39	124533.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.43309271948576	17.85376109806578	12.745271258927659	24.967874923520807
2	15.73694587896324	19.34509638745437	39.65669218278822	25.261265550794178
3	18.98908996793465	23.60926543919395	29.505950676863936	27.895693916007463
4	13.072744813719353	15.666594739905404	36.92702628216065	34.333634164214594
5	14.046323712977562	37.29656466440456	34.754839934395285	13.902271688222587
6	33.60573632382851	35.55234200280862	17.065398679142383	13.776522994220485
7	28.91228860037311	31.824390523635397	21.072490504479642	18.190830371511854
8	26.44742830577983	35.1913289740677	19.862193843747292	18.49904887640518
9	26.779634341953685	15.405456596750419	19.259192158204765	38.55571690309113
10-11	25.52225464482658	25.160966207505446	28.27751173550679	21.039267412161188
12-13	25.99693386049416	22.67204402843325	27.88612850239173	23.444893608680857
14-15	23.130384664554676	24.34305452452937	24.935430555008896	27.591130255907053
16-17	23.441331043662288	27.468395248312262	25.828050748205637	23.262222959819812
18-19	23.4819756266922	26.87257308187204	27.013401247261104	22.632050044174655
20-21	23.971793206923486	26.097232079164247	26.915194302634127	23.015780411278143
22-23	23.80948022323242	25.93457562545184	26.587650822461335	23.668293328854407
24-25	23.622638573625245	26.096675087256838	26.647613555929805	23.63307278318811
26-27	23.483291447111636	26.306855455395024	26.61727666702307	23.59257643047027
28-29	23.626568538964616	26.514839791620975	26.426551091690005	23.432040577724404
30-31	23.908801345758928	26.199779011254563	26.333150065694205	23.5582695772923
32-33	23.573375864408103	26.294065292398088	26.246422192290236	23.886136650903573
34-35	23.93791245877509	26.33079479702232	26.320378959581365	23.410913784621222
36-37	23.963425699302032	26.176015347054797	26.304766523169263	23.555792430473907
38-39	23.83067763502322	26.234614921838794	26.305054328622784	23.6296531145152
40-41	23.99339633531989	26.085634452146994	26.35355377776314	23.567415434769977
42-43	23.760348043706323	26.063977687342447	26.28478384918718	23.890890419764055
44-45	23.717116482742686	26.178039513399128	26.4059273766169	23.69891662724129
46-47	23.91154019017282	26.15262147429858	26.13685040421772	23.798987931310876
48-49	23.643556754167697	26.314055230201234	26.17682718176015	23.865560833870923
50-51	23.790494255090312	26.28321015644651	26.239366499656313	23.686929088806863
52-53	23.981779501710175	26.146201082654002	26.218062165491396	23.653957250144426
54-55	23.525131787954578	25.951292480162653	26.590479724354033	23.933096007528732
56-57	23.562121624527435	26.144485271811384	26.413372276151264	23.880020827509917
58-59	23.638132429530508	26.067685864834605	26.442911783698687	23.851269921936197
60-61	23.648549916085585	26.00379372644206	26.563284309379764	23.78437204809259
62-63	23.627066216744915	26.02160052327856	26.618786067097027	23.7325471928795
64-65	23.41498758367779	25.882006656169583	26.587633376700165	24.115372383452463
66-67	23.398518591873714	26.0856110120816	26.429998361734018	24.08587203431067
68-69	23.603829907274996	25.905573249818993	26.480340520420192	24.01025632248582
70-71	23.49480038829961	25.71558933083716	26.49629891429828	24.293311366564946
72-73	23.464781646729307	25.879392914854098	26.435748056669183	24.22007738174741
74-75	23.418088960170177	25.98918198607366	26.437337200657424	24.155391853098738
76-77	23.44131328345772	25.671321258523072	26.581462794157893	24.30590266386131
78-79	23.388074437712824	25.663449958122676	26.602602507748834	24.34587309641567
80-81	23.498794211297103	25.816641550208487	26.43929227991051	24.245271958583906
82-83	23.432534223542863	25.843100570443333	26.516856508740073	24.207508697273735
84-85	23.383522576600228	25.820322906497793	26.555194793781162	24.240959723120813
86-87	23.377391526083684	25.824224122735746	26.551483174605313	24.246901176575264
88-89	23.268290453357775	25.648542210255393	26.56196235065434	24.521204985732492
90-91	23.25097460873096	25.61189651727482	26.669951415175753	24.467177458818473
92-93	23.329276776751147	25.488464495244568	26.849553623362098	24.33270510464219
94-95	23.223469194793868	25.313665501633203	26.890152524450645	24.572712779122284
96-97	23.25017757283391	25.1801183248132	26.91430853306646	24.65539556928643
98-99	23.105064448515254	25.15562745292576	26.904191947226163	24.835116151332823
100-101	23.248256611605754	24.982869586567677	26.995223053118195	24.773650748708377
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	31.0
2	44.0
3	69.5
4	66.5
5	73.0
6	115.5
7	223.5
8	386.5
9	476.5
10	460.5
11	498.5
12	574.5
13	616.0
14	712.0
15	830.0
16	917.0
17	1025.5
18	1154.5
19	1240.0
20	1350.5
21	1498.5
22	1668.5
23	1872.0
24	2189.0
25	2727.0
26	3528.5
27	4964.5
28	7320.0
29	10798.0
30	15586.5
31	22140.0
32	31123.0
33	38590.0
34	44222.5
35	51395.5
36	60796.5
37	70502.5
38	79439.0
39	87825.0
40	95320.5
41	102656.5
42	109826.0
43	117442.0
44	124682.5
45	132846.5
46	137834.5
47	140644.0
48	143961.5
49	144515.0
50	142524.0
51	139085.0
52	135842.5
53	133127.0
54	130514.5
55	123651.0
56	112654.0
57	101224.0
58	91536.0
59	80677.0
60	66100.0
61	53704.0
62	44287.0
63	33630.0
64	23672.5
65	17033.5
66	12811.5
67	10020.5
68	7653.5
69	5639.5
70	4125.0
71	2962.0
72	2044.0
73	1445.0
74	1108.0
75	776.0
76	518.0
77	345.0
78	229.0
79	168.0
80	137.5
81	110.0
82	83.0
83	78.0
84	70.5
85	63.5
86	59.5
87	49.0
88	41.5
89	39.0
90	27.5
91	25.0
92	27.5
93	24.5
94	22.0
95	16.5
96	15.0
97	12.0
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05459272757771458
2	0.07517937065869634
3	0.07292246460241092
4	0.07148902426936479
5	0.068042667723956
6	0.010644056941129826
7	0.01332794522428004
8	0.018207742102734972
9	0.005733761332184548
10-11	0.012794217440699031
12-13	0.023224783268396453
14-15	0.04105129124000213
16-17	0.0279368371291545
18-19	0.011025291072259117
20-21	0.020693388637697957
22-23	0.03826065740013572
24-25	0.03492104641144312
26-27	0.02130336324750482
28-29	0.011467522664369095
30-31	0.01151327076010461
32-33	0.008570143267786478
34-35	0.005001791800416307
36-37	6.099746098068668E-4
38-39	0.0036750970240863722
40-41	0.0012046998543685618
42-43	0.23961327609738242
44-45	0.20803184067463193
46-47	0.21384184883304233
48-49	0.22152752891660882
50-51	0.21263714897867378
52-53	0.22065831509763406
54-55	0.21629699663751495
56-57	0.21930112159081377
58-59	0.22759677628418715
60-61	0.2328578072937714
62-63	0.21791342935350314
64-65	0.21725770664796076
66-67	0.21571752075819844
68-69	0.2101972505394463
70-71	0.20158135917592432
72-73	0.21330812104946134
74-75	0.2124999046914672
76-77	0.20591217890555308
78-79	0.2246231500613787
80-81	0.23573993732510884
82-83	0.23438274381828855
84-85	0.2289387204257623
86-87	0.24973885462017645
88-89	0.24190068088415817
90-91	0.2486713990530144
92-93	0.24363910852210777
94-95	0.24714646252849726
96-97	0.25423741736750205
98-99	0.2474514498334007
100-101	0.25393243006259864
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3278825.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.882550618304975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.10273907741269	49.56961805622868
2	11.213316510652154	13.878172531390137
3	3.609135981446597	6.700276201806446
4	1.6207765193364716	4.011911399951974
5	0.9052963807337303	2.801102455266168
6	0.568734586075557	2.1116848086720834
7	0.3818001850112768	1.653873849252698
8	0.26327814121448684	1.3033858320318983
9	0.20151612528418367	1.1223298640972863
>10	1.061130596537	11.54351896203015
>50	0.05094483195085756	2.1447776848260456
>100	0.019452318851826248	2.0656941888376914
>500	0.0011371354319213139	0.4896753468985442
>1k	7.416100612635193E-4	0.6039788187103245
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.149619147103002E-5
2	0.0	0.0	0.0	3.049873049034334E-5	9.149619147103002E-5
3	0.0	0.0	0.0	3.049873049034334E-5	9.149619147103002E-5
4	0.0	0.0	0.0	3.049873049034334E-5	9.149619147103002E-5
5	0.0	0.0	0.0	3.049873049034334E-5	9.149619147103002E-5
6	0.0	0.0	0.0	3.049873049034334E-5	1.2199492196137336E-4
7	0.0	0.0	0.0	3.049873049034334E-5	1.2199492196137336E-4
8	0.0	0.0	0.0	3.049873049034334E-5	1.524936524517167E-4
9	0.0	0.0	0.0	6.099746098068668E-5	2.1349111343240337E-4
10-11	0.0	0.0	0.0	7.624682622585835E-5	2.1349111343240337E-4
12-13	0.0	0.0	0.0	1.2199492196137336E-4	3.2023667014860507E-4
14-15	0.0	0.0	0.0	1.2199492196137336E-4	3.659847658841201E-4
16-17	0.0	0.0	0.0	1.3724428720654502E-4	4.2698222686480674E-4
18-19	0.0	0.0	0.0	1.524936524517167E-4	4.574809573551501E-4
20-21	0.0	0.0	0.0	1.524936524517167E-4	5.032290530906651E-4
22-23	0.0	0.0	0.0	1.9824174818723172E-4	6.252239750520385E-4
24-25	0.0	0.0	0.0	3.2023667014860507E-4	7.014708012778968E-4
26-27	0.0	0.0	0.0	7.167201665230685E-4	7.167201665230685E-4
28-29	0.0	0.0	0.0	0.002714387013640557	7.319695317682402E-4
30-31	0.0	4.574809573551501E-5	0.0	0.011498021394859437	7.472188970134119E-4
32-33	0.0	6.099746098068668E-5	0.0	0.03400608449673282	8.539644537296135E-4
34-35	0.0	6.099746098068668E-5	0.0	0.0643828200651148	9.149619147103002E-4
36-37	0.0	6.099746098068668E-5	0.0	0.11385176092045168	9.149619147103002E-4
38-39	0.0	6.099746098068668E-5	0.0	0.19575610165226873	9.149619147103002E-4
40-41	0.0	6.099746098068668E-5	0.0	0.2903479142680686	9.149619147103002E-4
42-43	0.0	6.099746098068668E-5	0.0	0.389697528840362	9.149619147103002E-4
44-45	0.0	6.099746098068668E-5	0.0	0.510243761103444	9.454606452006435E-4
46-47	3.049873049034334E-5	6.099746098068668E-5	0.0	0.6498821986534811	9.759593756909869E-4
48-49	3.049873049034334E-5	6.099746098068668E-5	0.0	0.7954831380143801	0.0010979542976523602
50-51	3.049873049034334E-5	6.099746098068668E-5	0.0	0.9559217097588313	0.0011894504891233903
52-53	3.049873049034334E-5	6.099746098068668E-5	0.0	1.1215450656866408	0.001250447950104077
54-55	3.049873049034334E-5	1.2199492196137336E-4	0.0	1.3116894009286864	0.0013571935068202785
56-57	3.049873049034334E-5	1.8299238294206005E-4	0.0	1.5247077840384895	0.0013724428720654502
58-59	3.049873049034334E-5	1.8299238294206005E-4	0.0	1.7487362088553065	0.001387692237310622
60-61	3.049873049034334E-5	1.8299238294206005E-4	0.0	1.985863838417726	0.0014486896982913086
62-63	3.049873049034334E-5	1.8299238294206005E-4	0.0	2.23406250714814	0.0015554352550075103
64-65	3.049873049034334E-5	2.134911134324034E-4	0.0	2.504006770718169	0.0015706846202526818
66-67	3.049873049034334E-5	2.4398984392274672E-4	0.0	2.8192111503358674	0.0016011833507430253
68-69	3.049873049034334E-5	2.4398984392274672E-4	0.0	3.156908343690194	0.001616432715988197
70-71	3.049873049034334E-5	2.4398984392274672E-4	0.0	3.5151921801255024	0.0016774301769688837
72-73	3.049873049034334E-5	2.4398984392274672E-4	0.0	3.8782795666130396	0.0017689263684399137
74-75	3.049873049034334E-5	2.4398984392274672E-4	0.0	4.260596402674739	0.0017994250989302571
76-77	3.049873049034334E-5	2.4398984392274672E-4	0.0	4.688295349706069	0.0018146744641754286
78-79	3.049873049034334E-5	2.4398984392274672E-4	0.0	5.152928259361204	0.0018604225599109438
80-81	4.574809573551501E-5	2.4398984392274672E-4	0.0	5.644705039152745	0.0019061706556464588
82-83	6.099746098068668E-5	2.4398984392274672E-4	0.0	6.1591881237943475	0.001982417481872317
84-85	6.099746098068668E-5	2.4398984392274672E-4	0.0	6.694212103421195	0.0019976668471174885
86-87	6.099746098068668E-5	2.7448857441309004E-4	0.0	7.257782894787004	0.0020434149428530035
88-89	6.099746098068668E-5	2.7448857441309004E-4	0.0	7.870182153667853	0.002073913673343347
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2410	0.0	46.668842	1
GTATCAA	4320	0.0	38.99782	1
TCAACGC	4735	0.0	34.681534	4
CAACGCA	4965	0.0	33.2646	5
ATCAACG	5105	0.0	32.074425	3
AACGCAG	5360	0.0	30.97468	6
TATCAAC	5375	0.0	30.110048	2
GTACATG	8425	0.0	28.896366	1
TACATGG	8830	0.0	26.659777	2
ACATGGG	8915	0.0	25.926456	3
ACGCAGA	6565	0.0	24.572002	7
CGCAGAG	6780	0.0	23.30437	8
CATGGGG	7705	0.0	23.284197	4
ATGGGGG	4655	0.0	18.861418	5
GCAGAGT	8020	0.0	18.695435	9
GAGTACA	6365	0.0	18.415958	1
TACCGTG	395	1.8189894E-11	18.017334	7
GTATATA	1240	0.0	17.987545	1
GTATAGC	915	0.0	17.115461	1
AGAGTAC	6660	0.0	17.027718	10-11
>>END_MODULE