FastQCFastQC Report
Mon 15 Aug 2016
SRR2921671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278825
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23000.046.2895161
GTATCAA43100.043.559441
TCAACGC51600.035.7755364
ATCAACG53600.034.2619863
CAACGCA53950.034.244885
AACGCAG57150.032.573246
TATCAAC57550.032.4051172
TATACCG2155.820766E-1126.5082025
ACGCAGA69650.026.1819027
GTACATG81500.025.018551
CGCAGAG72650.024.6431858
TACATGG85100.022.9181022
ACATGGG86350.021.9268843
CATGGGG71200.019.9286294
GCAGAGT84550.019.8270849
AGAGTAC70300.018.34054610-11
GTACCGT4151.8189894E-1218.3089316
GAGTACA63350.017.331171
ATATCGC2304.283458E-516.517846
TACACTG36700.016.1763845
Produced by FastQC (version 0.11.5)