##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921671_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3278825
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4524895351231	33.0	31.0	34.0	29.0	34.0
2	31.59643530838029	34.0	31.0	34.0	29.0	34.0
3	31.670559727951325	34.0	31.0	34.0	28.0	34.0
4	35.15549259262083	37.0	35.0	37.0	33.0	37.0
5	34.91952543975357	37.0	35.0	37.0	32.0	37.0
6	34.8950648479257	37.0	35.0	37.0	32.0	37.0
7	34.78231561611248	37.0	35.0	37.0	32.0	37.0
8	34.728900444519	37.0	35.0	37.0	32.0	37.0
9	36.32927130908176	39.0	37.0	39.0	32.0	39.0
10-11	36.2256346404581	39.0	37.0	39.0	32.0	39.0
12-13	36.15000343110718	39.0	37.0	39.0	32.0	39.0
14-15	37.40160347075553	40.0	38.0	41.0	32.0	41.0
16-17	37.371614679038984	40.0	38.0	41.0	32.0	41.0
18-19	37.334289570196646	40.0	38.0	41.0	31.5	41.0
20-21	37.279691352847436	40.0	38.0	41.0	31.0	41.0
22-23	37.19485684658376	40.0	38.0	41.0	31.0	41.0
24-25	37.115734600047276	40.0	37.5	41.0	31.0	41.0
26-27	36.99278400036599	40.0	37.0	41.0	30.5	41.0
28-29	36.872179363034014	40.0	37.0	41.0	30.0	41.0
30-31	36.723071374654026	40.0	37.0	41.0	30.0	41.0
32-33	36.67278156046754	40.0	37.0	41.0	30.0	41.0
34-35	36.796029370277466	40.0	37.0	41.0	30.0	41.0
36-37	36.79419746403056	40.0	37.0	41.0	30.0	41.0
38-39	36.68857944538058	40.0	37.0	41.0	30.0	41.0
40-41	36.60746197189542	40.0	37.0	41.0	30.0	41.0
42-43	36.4915440744779	40.0	37.0	41.0	30.0	41.0
44-45	36.36667403719321	40.0	36.5	41.0	29.5	41.0
46-47	36.21869739922076	40.0	36.0	41.0	29.0	41.0
48-49	36.07631865073616	40.0	36.0	41.0	28.0	41.0
50-51	35.92628258598735	40.0	35.5	41.0	28.0	41.0
52-53	35.758249830350806	39.5	35.0	41.0	28.0	41.0
54-55	35.550648174269746	39.0	35.0	41.0	27.5	41.0
56-57	35.32935350316043	39.0	35.0	41.0	27.0	41.0
58-59	35.095958765716375	39.0	35.0	41.0	26.5	41.0
60-61	34.82521513042019	38.0	35.0	40.0	26.0	41.0
62-63	34.53025275822894	37.5	34.0	40.0	26.0	41.0
64-65	34.20200330911226	37.0	34.0	40.0	26.0	41.0
66-67	33.8576023423025	36.5	34.0	39.5	25.0	41.0
68-69	33.497885980496065	36.0	34.0	39.0	24.5	41.0
70-71	33.094352702568756	35.5	33.0	39.0	23.5	40.0
72-73	32.73067897798754	35.0	33.0	38.0	23.0	40.0
74-75	32.321163984049164	35.0	33.0	37.0	22.0	39.0
76-77	29.595381424748197	33.0	29.0	34.5	17.5	36.5
78-79	31.16057230867765	34.5	31.5	36.0	19.5	38.0
80-81	31.334279963096535	35.0	32.0	36.0	20.0	37.0
82-83	31.147622700205105	35.0	32.0	35.0	20.0	37.0
84-85	30.936332527658536	35.0	32.0	35.0	18.5	36.5
86-87	30.718922479851777	35.0	32.0	35.0	17.5	36.0
88-89	30.437229800309563	35.0	32.0	35.0	11.5	36.0
90-91	30.230091267450995	34.5	31.5	35.0	8.5	35.5
92-93	30.066847117488734	34.0	31.0	35.0	4.5	35.0
94-95	29.955018184868052	34.0	31.0	35.0	2.0	35.0
96-97	29.746507666618378	34.0	31.0	35.0	2.0	35.0
98-99	29.5394339435621	34.0	31.0	35.0	2.0	35.0
100-101	28.42947717551257	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	53243.0
3	18291.0
4	10332.0
5	7398.0
6	6547.0
7	6690.0
8	6753.0
9	7503.0
10	8575.0
11	9294.0
12	9867.0
13	10777.0
14	11465.0
15	12462.0
16	13320.0
17	14152.0
18	13233.0
19	12274.0
20	11869.0
21	11901.0
22	12640.0
23	14103.0
24	17136.0
25	20193.0
26	24443.0
27	29506.0
28	35460.0
29	43216.0
30	52890.0
31	65207.0
32	82214.0
33	107637.0
34	152610.0
35	227225.0
36	394429.0
37	770053.0
38	856261.0
39	117653.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04591333272639	19.418230299267947	13.423186834874409	26.11266953313125
2	14.96132298774468	20.76898982896596	38.28553007315739	25.984157110131967
3	17.648653891672016	24.637485328602697	29.46379550573313	28.25006527399216
4	12.774502845566518	16.85168923772246	35.985596330639	34.38821158607202
5	13.787046330220655	37.53955930310953	34.87225403372188	13.801140332947934
6	33.50809452353939	36.21122009540192	16.79189693664678	13.488788444411911
7	28.70696057276616	31.946993206407782	21.15754881703049	18.188497403795566
8	26.27398534536	35.77159500735782	19.66866179195291	18.285757855329273
9	26.479188879915483	15.394452401226783	19.17922401446372	38.94713470439402
10-11	25.458110350492714	25.436460494123263	28.274666647137554	20.830762508246476
12-13	25.91825829811737	22.828453929711213	27.92398231556757	23.329305456603848
14-15	22.999811355830026	24.51392100948574	24.935419490801042	27.550848143883194
16-17	23.35173423406251	27.685009111495734	25.784526469085723	23.178730185356034
18-19	23.41093756719358	27.019741836351585	27.029211842356883	22.54010875409795
20-21	23.876127349512128	26.199906124735765	26.92989457484539	22.994071950906715
22-23	23.71518760531593	26.03211516320633	26.64690857243067	23.605788659047068
24-25	23.526676735354147	26.270856639538952	26.630941328749557	23.57152529635734
26-27	23.397003576828617	26.445062398160267	26.631002587119912	23.526931437891204
28-29	23.58077357297309	26.630739206626153	26.435445024748805	23.353042195651952
30-31	23.795725887864993	26.33857506920949	26.33863607696922	23.527062965956297
32-33	23.488040009132362	26.381997720570215	26.28031861993383	23.849643650363596
34-35	23.847577693006535	26.38712592799779	26.378997201918626	23.386299177077053
36-37	23.870292118149	26.277609917740026	26.38413110356101	23.467966860549964
38-39	23.75620839782544	26.328196838806583	26.366259254458534	23.54933550890944
40-41	23.866241153332876	26.186027935101848	26.408280216603714	23.539450694961563
42-43	23.706351339027133	26.160159241759356	26.2786114660289	23.854877953184605
44-45	23.693226330327935	26.229690451669992	26.38071530237225	23.69636791562983
46-47	23.828151210781808	26.23778939753098	26.161021353533158	23.773038038154056
48-49	23.53990606462255	26.37076275483788	26.239331920200225	23.84999926033935
50-51	23.6927395659883	26.33031047173202	26.255388318030437	23.72156164424924
52-53	23.93235513129083	26.16081241428761	26.212920361458664	23.693912092962897
54-55	23.44793289519113	26.06649236951809	26.518593390291006	23.966981344999773
56-57	23.491205638542077	26.191354910574034	26.367986364820357	23.94945308606353
58-59	23.530493739095977	26.13452528512694	26.427678440835788	23.90730253494129
60-61	23.532613469717415	26.14523526010009	26.483613047583958	23.838538222598537
62-63	23.54710656773631	26.114804441921326	26.514346131988013	23.82374285835435
64-65	23.264809354771735	26.006460078208654	26.49867758022123	24.23005298679838
66-67	23.303129585187754	26.240258955535428	26.260857968735884	24.195753490540934
68-69	23.495503621544415	26.053287066095894	26.29961067291429	24.151598639445403
70-71	23.378912947042714	25.94332892476579	26.309046231056243	24.368711897135256
72-73	23.330566663000486	26.090209724391126	26.177877732493744	24.401345880114647
74-75	23.318017787428847	26.20566070142373	26.147590570033007	24.328730941114415
76-77	23.318285100629453	25.86617262650252	26.34560577441285	24.469936498455183
78-79	23.268452000934676	25.955214069324956	26.270414909127926	24.505919020612442
80-81	23.40918870913103	26.13216933251542	26.023368521926493	24.43527343642706
82-83	23.291473352548817	26.193107075479755	26.111759559133986	24.40366001283744
84-85	23.234105711753443	26.14929503069532	26.15199556563009	24.464603691921145
86-87	23.190510509942285	26.21406927929579	26.04143793229366	24.553982278468265
88-89	23.04305048054533	26.140781833078652	26.038308728827257	24.777858957548755
90-91	23.013403833403054	26.06337817592541	26.10196763410525	24.82125035656629
92-93	23.07665196194583	26.006132620785273	26.195188957925925	24.72202645934297
94-95	22.929080602515274	25.91917710884845	26.211491603069035	24.940250685567243
96-97	22.84464818082363	25.83859887585389	26.272450397849745	25.044302545472735
98-99	22.71127391484889	25.833473247560956	26.205164733562896	25.250088104027256
100-101	22.689633066052135	25.81999799317986	26.17358567215818	25.31678326860983
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	23.5
2	32.5
3	48.0
4	38.0
5	39.5
6	82.5
7	214.5
8	346.0
9	372.0
10	323.0
11	273.0
12	268.5
13	285.5
14	340.5
15	376.0
16	415.5
17	489.0
18	554.0
19	653.0
20	773.5
21	880.0
22	1062.0
23	1335.0
24	1705.5
25	2296.0
26	3197.5
27	4746.0
28	7236.5
29	10865.0
30	15799.5
31	22424.0
32	31773.0
33	39267.0
34	44727.0
35	52111.0
36	61457.5
37	71253.0
38	80173.0
39	88406.0
40	95882.0
41	103387.5
42	110464.0
43	118156.5
44	126020.5
45	134473.0
46	139237.5
47	141699.5
48	145354.0
49	146176.0
50	143926.0
51	140239.0
52	137145.5
53	134433.0
54	131632.0
55	124588.0
56	113356.5
57	101391.0
58	91145.0
59	80160.0
60	65246.5
61	52391.0
62	42679.5
63	31923.0
64	22092.5
65	15706.5
66	11699.0
67	9042.5
68	6699.5
69	4814.5
70	3519.0
71	2477.0
72	1671.0
73	1163.5
74	820.0
75	517.5
76	309.0
77	183.0
78	107.0
79	67.5
80	41.0
81	30.5
82	16.5
83	16.0
84	14.5
85	10.0
86	7.0
87	4.5
88	3.5
89	2.0
90	2.0
91	2.0
92	1.5
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16990842756170274
2	1.2199492196137335E-4
3	0.010278072175245704
4	0.006587725785914161
5	0.025466439959436687
6	0.001982417481872317
7	0.0
8	0.0
9	3.0498730490343337E-5
10-11	0.050841383727402344
12-13	2.7448857441309004E-4
14-15	0.005047539896151823
16-17	0.0
18-19	0.0015859339854978536
20-21	1.8299238294206005E-4
22-23	0.0
24-25	3.964834963744634E-4
26-27	0.0025161452654533256
28-29	0.005535519583997316
30-31	0.016881047326405038
32-33	0.011391275838143238
34-35	0.010018832966077787
36-37	0.004102079250951179
38-39	0.0
40-41	0.0036140995631056857
42-43	0.008783634381218881
44-45	0.006877463725572423
46-47	0.0035683514673701703
48-49	0.008981876129406112
50-51	0.0026991376483953855
52-53	0.0016621808117237122
54-55	0.004056331155215664
56-57	0.007533186431114804
58-59	0.015386609532378215
60-61	0.0073501940481727444
62-63	0.02116611896029828
64-65	0.03996858630759494
66-67	0.060052000335486036
68-69	0.04977392816024032
70-71	0.06863739296851769
72-73	0.03411283005344903
74-75	0.02720486759738626
76-77	0.2307533948899377
78-79	0.0859606718870327
80-81	0.06694471342630363
82-83	0.02784534093768347
84-85	0.051741096276867474
86-87	0.06677697040860675
88-89	0.2950599681288266
90-91	0.29881131197913885
92-93	0.0536625162977591
94-95	0.010232324079510191
96-97	0.01569159683728165
98-99	9.302112799554719E-4
100-101	1.8299238294206005E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3278825.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.197307421622384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.76965843860981	47.41721344525187
2	12.009743049878466	14.459083888564534
3	3.730042565615218	6.736155570542291
4	1.7590297398703238	4.235554160590013
5	0.9737461231300839	2.930844736233731
6	0.6169895228962574	2.228466479142368
7	0.408358059081985	1.7207438934458703
8	0.29193208219693034	1.4058820238594358
9	0.20970263118638674	1.1361180380985016
>10	1.1510770981877017	12.101200359783187
>50	0.056296987092665694	2.285310212912643
>100	0.02139009893580386	2.1866129499640454
>500	0.0013218421583418782	0.5648034830128995
>1k	7.117611599725246E-4	0.5920107585986589
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.049873049034334E-5
2	0.0	0.0	0.0	0.0	3.049873049034334E-5
3	0.0	0.0	0.0	0.0	3.049873049034334E-5
4	0.0	0.0	0.0	0.0	3.049873049034334E-5
5	0.0	3.049873049034334E-5	0.0	0.0	3.049873049034334E-5
6	0.0	3.049873049034334E-5	0.0	0.0	6.099746098068668E-5
7	0.0	3.049873049034334E-5	0.0	0.0	6.099746098068668E-5
8	0.0	3.049873049034334E-5	0.0	0.0	9.149619147103002E-5
9	0.0	3.049873049034334E-5	0.0	3.049873049034334E-5	2.1349111343240337E-4
10-11	0.0	3.049873049034334E-5	0.0	9.149619147103002E-5	2.1349111343240337E-4
12-13	0.0	3.049873049034334E-5	0.0	1.2199492196137336E-4	3.2023667014860507E-4
14-15	0.0	3.049873049034334E-5	0.0	1.3724428720654502E-4	3.964834963744634E-4
16-17	0.0	3.049873049034334E-5	0.0	1.524936524517167E-4	4.574809573551501E-4
18-19	0.0	3.049873049034334E-5	0.0	1.8299238294206005E-4	5.794758793165234E-4
20-21	0.0	6.099746098068668E-5	0.0	1.8299238294206005E-4	6.404733402972101E-4
22-23	0.0	6.099746098068668E-5	0.0	2.439898439227467E-4	7.624682622585834E-4
24-25	0.0	6.099746098068668E-5	0.0	3.812341311292917E-4	8.539644537296135E-4
26-27	0.0	6.099746098068668E-5	0.0	7.472188970134118E-4	8.692138189747851E-4
28-29	0.0	6.099746098068668E-5	0.0	0.0028516313008471023	8.844631842199568E-4
30-31	0.0	6.099746098068668E-5	0.0	0.0117877593345177	8.997125494651286E-4
32-33	0.0	6.099746098068668E-5	0.0	0.03516503625536587	9.454606452006435E-4
34-35	0.0	6.099746098068668E-5	0.0	0.06677697040860675	0.0010369568366716735
36-37	0.0	7.624682622585835E-5	0.0	0.11792334144091252	0.0010369568366716735
38-39	0.0	9.149619147103002E-5	0.0	0.20104763139234327	0.0010522062019168452
40-41	0.0	9.149619147103002E-5	0.0	0.29795734752540926	0.001067455567162017
42-43	0.0	9.149619147103002E-5	0.0	0.3989843922746716	0.0010827049324071887
44-45	0.0	9.149619147103002E-5	0.0	0.5215892888458518	0.001113203662897532
46-47	0.0	9.149619147103002E-5	0.0	0.6631491464167805	0.0011742011238782186
48-49	0.0	9.149619147103002E-5	0.0	0.8112052335821521	0.0012504479501040768
50-51	0.0	9.149619147103002E-5	0.0	0.9741294518615663	0.001296196045839592
52-53	0.0	9.149619147103002E-5	0.0	1.1413387417748737	0.0014181909678009654
54-55	0.0	1.3724428720654502E-4	0.0	1.333328250211585	0.0015554352550075103
56-57	0.0	1.524936524517167E-4	0.0	1.5481460584203184	0.0015706846202526818
58-59	0.0	1.8299238294206005E-4	0.0	1.7752243562861696	0.0016011833507430253
60-61	0.0	1.8299238294206005E-4	0.0	2.0159508360464495	0.001662180811723712
62-63	0.0	1.8299238294206005E-4	0.0	2.2679465967229113	0.001707928907459227
64-65	0.0	1.8299238294206005E-4	0.0	2.5405899979413356	0.0017384276379495704
66-67	0.0	1.8299238294206005E-4	0.0	2.8591034898172363	0.0017536770031947421
68-69	0.0	1.8299238294206005E-4	0.0	3.200384284004178	0.0017689263684399137
70-71	0.0	1.8299238294206005E-4	0.0	3.5628006984209284	0.0018604225599109436
72-73	0.0	1.8299238294206005E-4	0.0	3.9318048386235924	0.0020434149428530035
74-75	0.0	1.8299238294206005E-4	0.0	4.319733441095515	0.002073913673343347
76-77	0.0	1.8299238294206005E-4	0.0	4.753364391207216	0.002073913673343347
78-79	0.0	2.1349111343240337E-4	0.0	5.226491197303912	0.002073913673343347
80-81	0.0	2.1349111343240337E-4	0.0	5.7274785937035375	0.0020891630385885184
82-83	0.0	2.1349111343240337E-4	0.0	6.253154712435094	0.002226407325795064
84-85	0.0	2.1349111343240337E-4	0.0	6.7999969501269515	0.0022874047867757503
86-87	0.0	3.049873049034334E-4	0.0	7.375934976706595	0.002348402247756437
88-89	0.0	3.049873049034334E-4	0.0	8.000579475879317	0.0023789009782467807
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2300	0.0	46.289516	1
GTATCAA	4310	0.0	43.55944	1
TCAACGC	5160	0.0	35.775536	4
ATCAACG	5360	0.0	34.261986	3
CAACGCA	5395	0.0	34.24488	5
AACGCAG	5715	0.0	32.57324	6
TATCAAC	5755	0.0	32.405117	2
TATACCG	215	5.820766E-11	26.508202	5
ACGCAGA	6965	0.0	26.181902	7
GTACATG	8150	0.0	25.01855	1
CGCAGAG	7265	0.0	24.643185	8
TACATGG	8510	0.0	22.918102	2
ACATGGG	8635	0.0	21.926884	3
CATGGGG	7120	0.0	19.928629	4
GCAGAGT	8455	0.0	19.827084	9
AGAGTAC	7030	0.0	18.340546	10-11
GTACCGT	415	1.8189894E-12	18.308931	6
GAGTACA	6335	0.0	17.33117	1
ATATCGC	230	4.283458E-5	16.51784	6
TACACTG	3670	0.0	16.176384	5
>>END_MODULE