FastQCFastQC Report
Mon 15 Aug 2016
SRR2921670_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921670_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808665
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12900.051.5113141
GTATCAA24000.045.2885631
TCAACGC30650.034.533354
ATCAACG31400.033.858733
CAACGCA31200.033.767795
TATCAAC33200.032.7396282
AACGCAG33750.031.4802556
GTACATG51150.027.281361
ACGCAGA38250.026.4192357
CGCAGAG40250.024.8741788
TACATGG55050.024.5733222
ACATGGG56100.023.1814173
GGCGTAA1050.0022152922.5862796
CATGGGG48050.021.9292564
GTATATA4400.021.5745451
GAGTACA39300.020.7730481
GCAGAGT45900.020.2616839
TAAGGCG5350.018.6281495
AGAGTAC37950.018.56747410-11
AGTACAT41450.018.2074432
Produced by FastQC (version 0.11.5)