FastQCFastQC Report
Mon 15 Aug 2016
SRR2921670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808665
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.050.5495341
GTATCAA25400.045.665131
TCAACGC33050.034.6042064
ATCAACG34400.033.2471123
CAACGCA34600.032.9441955
TATCAAC35150.032.942752
AACGCAG37200.030.893576
GTACATG53150.028.1731761
TACATGG55250.026.6271532
ACGCAGA45150.024.7168887
ACATGGG59200.024.6901843
CATGGGG51250.023.5192324
CGCAGAG47600.023.14548
CCGAACG1252.2600449E-422.7948599
ATGGGGG30650.022.3139155
TATTCCG2056.8412373E-720.8512655
ACCGTCG1200.004771517619.7866558
GCAGAGT54500.019.1699899
GTATAGG3609.458745E-1118.4864751
AGAGTAC45150.017.61784410-11
Produced by FastQC (version 0.11.5)