##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921670_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1808665
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.334873511678502	33.0	31.0	34.0	28.0	34.0
2	31.470642711613262	34.0	31.0	34.0	28.0	34.0
3	31.54097359101879	34.0	31.0	34.0	28.0	34.0
4	35.02790345365228	37.0	35.0	37.0	32.0	37.0
5	34.76986230175295	37.0	35.0	37.0	32.0	37.0
6	34.741943367069084	37.0	35.0	37.0	32.0	37.0
7	34.62566810326954	37.0	35.0	37.0	32.0	37.0
8	34.56738423091064	37.0	35.0	37.0	32.0	37.0
9	36.14869641420606	39.0	37.0	39.0	32.0	39.0
10-11	36.03587148532205	39.0	37.0	39.0	31.0	39.0
12-13	35.95579197916695	39.0	37.0	39.0	31.0	39.0
14-15	37.18008614088291	40.0	38.0	41.0	31.0	41.0
16-17	37.14555957018021	40.0	38.0	41.0	31.0	41.0
18-19	37.105480561629705	40.0	38.0	41.0	31.0	41.0
20-21	37.0418900127995	40.0	37.5	41.0	31.0	41.0
22-23	36.95252852241847	40.0	37.0	41.0	30.5	41.0
24-25	36.86703424901792	40.0	37.0	41.0	30.0	41.0
26-27	36.73491470228041	40.0	37.0	41.0	30.0	41.0
28-29	36.614854879151196	40.0	37.0	41.0	30.0	41.0
30-31	36.45846024554022	40.0	36.5	41.0	30.0	41.0
32-33	36.4071221038722	40.0	36.5	41.0	30.0	41.0
34-35	36.53838328269746	40.0	37.0	41.0	30.0	41.0
36-37	36.531736944099656	40.0	37.0	41.0	30.0	41.0
38-39	36.42790677101619	40.0	37.0	41.0	30.0	41.0
40-41	36.346400521931926	40.0	36.5	41.0	29.5	41.0
42-43	36.22695330533847	40.0	36.0	41.0	29.0	41.0
44-45	36.098082563658835	40.0	36.0	41.0	28.5	41.0
46-47	35.93553891958986	40.0	36.0	41.0	28.0	41.0
48-49	35.78583679122447	40.0	35.0	41.0	27.5	41.0
50-51	35.62845081869777	39.5	35.0	41.0	27.0	41.0
52-53	35.454594963688685	39.0	35.0	41.0	27.0	41.0
54-55	35.23376495923789	39.0	35.0	41.0	26.0	41.0
56-57	35.000854497654345	39.0	35.0	41.0	26.0	41.0
58-59	34.74842273168331	38.0	34.5	40.0	26.0	41.0
60-61	34.46495592052702	38.0	34.0	40.0	25.5	41.0
62-63	34.158021800609845	37.0	34.0	40.0	25.0	41.0
64-65	33.81322273610647	37.0	34.0	40.0	23.5	41.0
66-67	33.460411408414494	36.0	34.0	39.0	23.0	41.0
68-69	33.093669087420835	36.0	33.0	39.0	22.0	40.5
70-71	32.700028750487235	35.0	33.0	38.5	22.0	40.0
72-73	32.34103385646319	35.0	33.0	37.0	21.0	39.5
74-75	31.93903542115317	35.0	32.5	37.0	20.0	39.0
76-77	29.23622976062455	33.0	28.5	34.5	13.0	36.5
78-79	30.809525534026477	34.5	31.5	35.5	16.5	37.0
80-81	30.996275153220743	35.0	32.0	35.5	18.0	37.0
82-83	30.824503432089415	35.0	32.0	35.0	16.5	37.0
84-85	30.635364481537486	35.0	32.0	35.0	13.5	36.0
86-87	30.430246341915172	35.0	32.0	35.0	8.5	36.0
88-89	30.165207487290353	35.0	31.0	35.0	3.5	36.0
90-91	29.96639952672275	34.0	31.0	35.0	2.0	35.0
92-93	29.80797134903368	34.0	31.0	35.0	2.0	35.0
94-95	29.699314411458175	34.0	31.0	35.0	2.0	35.0
96-97	29.49662319998452	34.0	31.0	35.0	2.0	35.0
98-99	29.293806758023184	34.0	31.0	35.0	2.0	35.0
100-101	28.158719276372352	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33819.0
3	11529.0
4	6443.0
5	4485.0
6	3878.0
7	4082.0
8	4135.0
9	4521.0
10	4975.0
11	5403.0
12	5910.0
13	6089.0
14	6600.0
15	7098.0
16	7530.0
17	7811.0
18	7570.0
19	7091.0
20	6856.0
21	6749.0
22	7083.0
23	8136.0
24	9833.0
25	11519.0
26	13689.0
27	16600.0
28	20194.0
29	24657.0
30	29473.0
31	37233.0
32	46879.0
33	62213.0
34	88088.0
35	130117.0
36	227248.0
37	433279.0
38	438768.0
39	51080.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.03552429149125	19.394925164507388	13.452611572060382	26.116938971940982
2	15.323622671970764	20.796277917690674	37.63101514100179	26.249084269336777
3	17.821698413681446	24.5157769245061	29.155736580877473	28.50678808093498
4	13.210372338807913	16.742224809943483	35.074681746225274	34.97272110502333
5	14.309146764662778	37.28143742378618	34.154354937273084	14.255060874277955
6	33.97063076023967	35.356599232456446	16.666012395037782	14.0067576122661
7	29.033308563669095	31.731432703918472	20.868276252526726	18.366982479885703
8	26.701572706941306	35.01676651010552	19.678934462711446	18.602726320241725
9	26.9183922046285	15.252316213689337	18.99342221298274	38.83586936869942
10-11	25.832504990798345	25.144054160049606	27.82434381165897	21.19909703749308
12-13	26.229543545798293	22.506260162916284	27.577310040201052	23.686886251084367
14-15	23.489993558500153	24.225955650411787	24.557043213340815	27.727007577747244
16-17	23.847229862909934	27.22170772365253	25.417531715381237	23.5135306980563
18-19	23.894631731898432	26.722576450215303	26.570444557974334	22.812347259911935
20-21	24.409709158171214	25.848202277538086	26.449005024981155	23.29308353930955
22-23	24.29825313145331	25.547544736034588	26.220361426798217	23.933840705713884
24-25	24.114120224210932	25.768969631560267	26.13689517176862	23.980014972460182
26-27	23.862089785451438	26.03016181197565	26.211875795326968	23.895872607245945
28-29	24.002075691408717	26.25387464239387	25.93989452347013	23.804155142727286
30-31	24.413057399159676	25.885418452360543	25.76904190219393	23.93248224628585
32-33	24.114426387509944	25.94320595052162	25.755392819195922	24.186974842772514
34-35	24.363860157059552	25.99165821576722	25.867327698417352	23.777153928755876
36-37	24.37130860478669	25.913995260417185	25.921542542897868	23.793153591898257
38-39	24.318406117219055	25.948337586009572	25.831981046794183	23.901275249977193
40-41	24.409380248398264	25.75659181543608	25.900848037487815	23.933179898677835
42-43	24.219870793368088	25.80519143366721	25.699993668781033	24.274944104183664
44-45	24.14573127592416	25.796085806049753	25.852485498505544	24.20569741952054
46-47	24.22951028149	25.75517392001504	25.788127768040297	24.227188030454663
48-49	24.002021586071404	25.88819014410436	25.843428822309995	24.26635944751424
50-51	24.09935127083572	25.87220151574715	25.890060504928364	24.13838670848877
52-53	24.318210971434638	25.726270358018027	25.770419943559304	24.18509872698803
54-55	23.819875201158027	25.65743876239536	26.072071040957034	24.45061499548958
56-57	23.892646581099108	25.778284640001104	25.85428632646996	24.474782452429828
58-59	24.00095775562246	25.6805414968913	25.906211282991627	24.41228946449461
60-61	24.034207920970005	25.692240957220193	26.07205250014031	24.201498621669497
62-63	24.00405800996585	25.699679058860227	26.014481753471745	24.28178117770218
64-65	23.76504295616991	25.51207930540019	26.002718552391592	24.720159186038313
66-67	23.80086334851542	25.691367565955765	25.86195164562326	24.645817439905553
68-69	23.803244120525935	25.62203592390392	25.937973557267462	24.636746398302684
70-71	23.697455187634063	25.459686713166	25.87316270544476	24.969695393755174
72-73	23.718176327700863	25.685613753312893	25.745566587466982	24.85064333151926
74-75	23.624424009007914	25.73111866686429	25.756281979528676	24.888175344599123
76-77	23.611568656515853	25.40228053867371	25.947382201491454	25.038768603318985
78-79	23.50851457239828	25.489986672506383	25.996885179091134	25.004613576004203
80-81	23.672851561419446	25.57761399308003	25.79194056835823	24.957593877142298
82-83	23.549976537485964	25.59302644862705	25.868714445027898	24.98828256885909
84-85	23.50668000159311	25.565277710168914	25.852978010647288	25.075064277590688
86-87	23.41125041044294	25.667379530328795	25.769924567019004	25.151445492209263
88-89	23.181093306924634	25.504839069204177	25.87269039383491	25.441377230036277
90-91	23.206084816571604	25.39096160862916	25.948167157783132	25.454786417016106
92-93	23.27368869709573	25.30150306917304	25.992644384449154	25.43216384928207
94-95	23.164426460248997	25.197822617233108	26.0353115548978	25.6024393676201
96-97	22.980202303948065	25.116904697062488	26.137548436685666	25.76534456230378
98-99	22.77883918680986	25.07131682695617	26.098461591199424	26.05138239503454
100-101	22.750817247551712	25.117317354675073	26.098152640417986	26.033712757355225
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	20.5
2	26.0
3	37.5
4	39.5
5	44.0
6	65.0
7	156.0
8	237.5
9	245.5
10	209.0
11	176.5
12	174.0
13	190.5
14	212.0
15	236.5
16	288.5
17	313.5
18	326.0
19	391.5
20	434.0
21	474.0
22	576.5
23	674.5
24	798.0
25	996.5
26	1321.5
27	1860.5
28	2842.5
29	4335.5
30	6537.0
31	9546.5
32	13543.5
33	16892.5
34	19739.0
35	23592.5
36	28710.0
37	33756.0
38	38559.5
39	43671.5
40	48262.0
41	52607.0
42	56665.5
43	60959.5
44	65213.5
45	70028.5
46	74035.5
47	76923.5
48	79724.5
49	81362.0
50	81953.5
51	81900.5
52	81510.0
53	80956.5
54	79457.0
55	75340.5
56	69326.0
57	62194.5
58	55786.0
59	49080.5
60	40520.0
61	33102.0
62	26930.0
63	20453.0
64	14417.5
65	10173.0
66	7412.0
67	5708.5
68	4297.0
69	3041.0
70	2217.0
71	1566.5
72	1050.0
73	748.0
74	531.0
75	345.5
76	236.5
77	141.0
78	78.0
79	46.0
80	30.5
81	19.5
82	12.0
83	13.0
84	8.0
85	5.0
86	3.0
87	1.5
88	1.0
89	1.0
90	1.5
91	2.0
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16426480304534008
2	0.0
3	0.011113169105389887
4	0.006855885418250478
5	0.02377444136973956
6	0.0021009971443025654
7	5.5289398534278047E-5
8	0.0
9	1.1057879706855609E-4
10-11	0.046332515971725004
12-13	3.317363912056683E-4
14-15	0.004423151882742243
16-17	0.0
18-19	0.0020457077457682877
20-21	8.293409780141707E-5
22-23	0.0
24-25	6.081833838770585E-4
26-27	0.0029579828215838756
28-29	0.006137123237304863
30-31	0.017195002944160473
32-33	0.012080733579739752
34-35	0.00989680233763577
36-37	0.0037043897017966293
38-39	0.0
40-41	0.004284928386406549
42-43	0.009205684855957295
44-45	0.007989318088203178
46-47	0.004146704890070854
48-49	0.01017324933030716
50-51	0.0028750487237824583
52-53	0.0022668653399054
54-55	0.004119060190803714
56-57	0.008431633276477401
58-59	0.014955782303522212
60-61	0.007547002899928954
62-63	0.021535220729101297
64-65	0.03967014344834449
66-67	0.058164447258060505
68-69	0.048184710822623315
70-71	0.06546264786458521
72-73	0.03190198295427843
74-75	0.02637304310085063
76-77	0.2237838405674904
78-79	0.08285116370361566
80-81	0.0631957825246798
82-83	0.025516057423569317
84-85	0.04870996010869896
86-87	0.06449508339023534
88-89	0.2885553709503971
90-91	0.2925362076448651
92-93	0.051446785336145724
94-95	0.00903981666035446
96-97	0.013684126137233818
98-99	5.805386846099194E-4
100-101	1.382234963356951E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1808665.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.17223863695816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.44583214922383	51.623891380286715
2	11.022952113986516	14.147350270850104
3	3.4579629080832377	6.657156628058019
4	1.597919028915422	4.101681649843876
5	0.9221816742436073	2.958923123309519
6	0.5949256709307097	2.2906627275730767
7	0.3867912003588544	1.7374880048472732
8	0.2834932378756488	1.455391657033606
9	0.21827996138043498	1.2606762394234154
>10	1.0303674610301925	11.078153214922521
>50	0.03013363980291392	1.2883421756326743
>100	0.008297006624554905	1.0350346690104724
>500	8.639475440615655E-4	0.3652482592087877
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.5289398534278047E-5	0.0	0.0	0.0
6	0.0	5.5289398534278047E-5	0.0	0.0	5.5289398534278047E-5
7	0.0	5.5289398534278047E-5	0.0	0.0	5.5289398534278047E-5
8	0.0	5.5289398534278047E-5	0.0	0.0	5.5289398534278047E-5
9	0.0	5.5289398534278047E-5	0.0	0.0	5.5289398534278047E-5
10-11	0.0	5.5289398534278047E-5	0.0	0.0	5.5289398534278047E-5
12-13	0.0	5.5289398534278047E-5	0.0	0.0	5.5289398534278047E-5
14-15	0.0	5.5289398534278047E-5	0.0	0.0	5.5289398534278047E-5
16-17	0.0	5.5289398534278047E-5	0.0	2.7644699267139023E-5	5.5289398534278047E-5
18-19	0.0	5.5289398534278047E-5	0.0	1.1057879706855609E-4	1.1057879706855609E-4
20-21	0.0	5.5289398534278047E-5	0.0	1.1057879706855609E-4	1.1057879706855609E-4
22-23	0.0	5.5289398534278047E-5	0.0	1.1057879706855609E-4	1.3822349633569513E-4
24-25	0.0	5.5289398534278047E-5	0.0	3.3173639120566824E-4	1.6586819560283415E-4
26-27	0.0	5.5289398534278047E-5	0.0	8.293409780141707E-4	1.6586819560283415E-4
28-29	0.0	5.5289398534278047E-5	0.0	0.0028197593252481806	1.6586819560283415E-4
30-31	0.0	5.5289398534278047E-5	0.0	0.012771851061418227	1.6586819560283415E-4
32-33	0.0	5.5289398534278047E-5	0.0	0.04096944431390003	1.6586819560283415E-4
34-35	0.0	5.5289398534278047E-5	0.0	0.07624408057876941	1.6586819560283415E-4
36-37	0.0	5.5289398534278047E-5	0.0	0.13028946764602622	1.6586819560283415E-4
38-39	0.0	5.5289398534278047E-5	0.0	0.22320330188288046	1.6586819560283415E-4
40-41	0.0	5.5289398534278047E-5	0.0	0.3251293080808221	1.6586819560283415E-4
42-43	0.0	1.1057879706855609E-4	0.0	0.4365374461273923	1.6586819560283415E-4
44-45	0.0	1.1057879706855609E-4	0.0	0.5743739166733475	1.6586819560283415E-4
46-47	0.0	1.1057879706855609E-4	0.0	0.736150696784645	1.6586819560283415E-4
48-49	0.0	1.1057879706855609E-4	0.0	0.9056127032922072	1.6586819560283415E-4
50-51	0.0	1.1057879706855609E-4	0.0	1.074577105212961	1.6586819560283415E-4
52-53	0.0	1.1057879706855609E-4	0.0	1.2566727392856056	1.6586819560283415E-4
54-55	0.0	1.1057879706855609E-4	0.0	1.4643120754810868	1.6586819560283415E-4
56-57	0.0	1.1057879706855609E-4	0.0	1.7019182656821468	1.6586819560283415E-4
58-59	0.0	1.1057879706855609E-4	0.0	1.9504717567929937	1.6586819560283415E-4
60-61	0.0	1.1057879706855609E-4	0.0	2.2088944055422095	2.2115759413711219E-4
62-63	5.5289398534278047E-5	1.3822349633569513E-4	0.0	2.481830521406673	2.2115759413711219E-4
64-65	5.5289398534278047E-5	1.6586819560283415E-4	0.0	2.7787345915357458	2.2115759413711219E-4
66-67	5.5289398534278047E-5	1.6586819560283415E-4	0.0	3.1228558080130924	2.2115759413711219E-4
68-69	5.5289398534278047E-5	1.6586819560283415E-4	0.0	3.4831491735617153	2.2115759413711219E-4
70-71	5.5289398534278047E-5	1.6586819560283415E-4	0.0	3.8851031009059165	2.2115759413711219E-4
72-73	5.5289398534278047E-5	1.6586819560283415E-4	0.0	4.290097945169504	2.764469926713902E-4
74-75	5.5289398534278047E-5	1.6586819560283415E-4	0.0	4.721576411331009	2.764469926713902E-4
76-77	5.5289398534278047E-5	1.6586819560283415E-4	0.0	5.206851462266368	2.764469926713902E-4
78-79	5.5289398534278047E-5	1.6586819560283415E-4	0.0	5.7349757970657915	2.764469926713902E-4
80-81	5.5289398534278047E-5	1.6586819560283415E-4	0.0	6.291435948614033	2.764469926713902E-4
82-83	5.5289398534278047E-5	1.6586819560283415E-4	0.0	6.862741303668727	2.764469926713902E-4
84-85	5.5289398534278047E-5	1.6586819560283415E-4	0.0	7.472445146005478	2.764469926713902E-4
86-87	5.5289398534278047E-5	1.6586819560283415E-4	0.0	8.118418833780716	2.764469926713902E-4
88-89	5.5289398534278047E-5	1.6586819560283415E-4	0.0	8.812411364182974	2.764469926713902E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1420	0.0	50.549534	1
GTATCAA	2540	0.0	45.66513	1
TCAACGC	3305	0.0	34.604206	4
ATCAACG	3440	0.0	33.247112	3
CAACGCA	3460	0.0	32.944195	5
TATCAAC	3515	0.0	32.94275	2
AACGCAG	3720	0.0	30.89357	6
GTACATG	5315	0.0	28.173176	1
TACATGG	5525	0.0	26.627153	2
ACGCAGA	4515	0.0	24.716888	7
ACATGGG	5920	0.0	24.690184	3
CATGGGG	5125	0.0	23.519232	4
CGCAGAG	4760	0.0	23.1454	8
CCGAACG	125	2.2600449E-4	22.794859	9
ATGGGGG	3065	0.0	22.313915	5
TATTCCG	205	6.8412373E-7	20.851265	5
ACCGTCG	120	0.0047715176	19.786655	8
GCAGAGT	5450	0.0	19.169989	9
GTATAGG	360	9.458745E-11	18.486475	1
AGAGTAC	4515	0.0	17.617844	10-11
>>END_MODULE