FastQCFastQC Report
Mon 15 Aug 2016
SRR2921669_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921669_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962921
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.043.3509861
GTATCAA31400.039.2996251
TCAACGC35100.033.6740724
CAACGCA35950.033.008255
ATCAACG37500.031.390233
AACGCAG39950.029.5696
TATCAAC39650.029.3284872
GTACATG61300.027.0989381
TACATGG64150.025.082442
ACATGGG66850.023.6437783
ACGCAGA48300.023.479327
GAGTACA47400.022.3291031
CGCAGAG50550.021.8725198
CATGGGG55900.020.6346874
GTATAGA7950.019.1041431
AGTACAT50650.018.9294782
TGGACCG5900.018.5036435
AGAGTAC47550.017.41544710-11
GTATAGC7750.017.1475091
CTATACT9500.016.9886464
Produced by FastQC (version 0.11.5)