##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921669_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2962921
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.460914415200406	31.0	31.0	34.0	26.0	34.0
2	30.504324955002176	31.0	31.0	34.0	26.0	34.0
3	30.566400521647388	33.0	31.0	34.0	26.0	34.0
4	34.03271197578336	37.0	35.0	37.0	30.0	37.0
5	34.034517963860665	37.0	35.0	37.0	30.0	37.0
6	34.078408097954686	37.0	35.0	37.0	30.0	37.0
7	33.98802803044698	37.0	35.0	37.0	30.0	37.0
8	33.95995674538741	37.0	35.0	37.0	30.0	37.0
9	35.528270919136894	39.0	37.0	39.0	30.0	39.0
10-11	35.43371659251124	39.0	36.0	39.0	30.0	39.0
12-13	35.32662632584534	39.0	35.0	39.0	29.0	39.0
14-15	36.473434492516	40.0	37.0	41.0	29.0	41.0
16-17	36.41438668125137	40.0	37.0	41.0	29.0	41.0
18-19	36.38101555863285	40.0	37.0	41.0	28.5	41.0
20-21	36.30102574452711	40.0	37.0	41.0	27.5	41.0
22-23	36.203388986746525	40.0	36.5	41.0	27.0	41.0
24-25	36.09545630814996	40.0	36.0	41.0	27.0	41.0
26-27	35.950979624498935	40.0	36.0	41.0	27.0	41.0
28-29	35.817668273976935	40.0	36.0	41.0	27.0	41.0
30-31	35.67178908921298	40.0	36.0	41.0	26.5	41.0
32-33	35.50207818568231	39.5	35.5	41.0	25.5	41.0
34-35	35.33072751517844	39.0	35.0	41.0	25.0	41.0
36-37	35.29916153687526	39.0	35.0	41.0	25.0	41.0
38-39	35.476090148876736	40.0	35.0	41.0	25.5	41.0
40-41	35.53800489449432	40.0	35.0	41.0	25.0	41.0
42-43	35.38131695040131	40.0	35.0	41.0	24.5	41.0
44-45	35.2594486994422	40.0	35.0	41.0	24.0	41.0
46-47	35.10633223093022	40.0	35.0	41.0	23.5	41.0
48-49	34.94650920493662	39.5	35.0	41.0	23.0	41.0
50-51	33.23368527206767	37.0	32.5	39.5	19.0	40.0
52-53	33.35005962021937	37.5	33.5	39.5	19.5	40.0
54-55	34.272131791566494	39.0	34.0	40.5	21.0	41.0
56-57	34.29329232875261	39.0	34.0	41.0	21.0	41.0
58-59	34.10599202611208	38.5	34.0	41.0	20.0	41.0
60-61	33.856572449957326	38.0	34.0	40.0	19.5	41.0
62-63	33.56801227572386	37.0	34.0	40.0	18.0	41.0
64-65	33.280095048096115	37.0	33.5	40.0	16.0	41.0
66-67	32.937247230013895	36.0	33.0	39.5	13.0	41.0
68-69	32.577417183920865	36.0	33.0	39.0	9.5	41.0
70-71	32.169166677073065	35.0	32.5	39.0	7.5	40.5
72-73	31.799723819838597	35.0	32.0	37.5	4.0	40.0
74-75	31.396505509259278	35.0	32.0	37.0	2.0	39.0
76-77	31.007143288666825	35.0	32.0	36.5	2.0	39.0
78-79	30.64768821038428	35.0	31.5	36.0	2.0	38.5
80-81	30.312932575657605	35.0	31.0	36.0	2.0	37.0
82-83	29.97919569910909	35.0	31.0	35.0	2.0	37.0
84-85	29.693520346981913	35.0	31.0	35.0	2.0	36.5
86-87	29.42972964854615	35.0	31.0	35.0	2.0	36.0
88-89	29.174975640592507	35.0	30.5	35.0	2.0	36.0
90-91	28.91470781704946	34.5	30.0	35.0	2.0	35.5
92-93	28.68693900377364	34.0	30.0	35.0	2.0	35.0
94-95	28.416747864691633	34.0	30.0	35.0	2.0	35.0
96-97	28.141283213423513	34.0	29.0	35.0	2.0	35.0
98-99	27.83335718367111	34.0	29.0	35.0	2.0	35.0
100-101	26.72882824077996	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	95326.0
3	16735.0
4	11607.0
5	8955.0
6	8138.0
7	8328.0
8	9072.0
9	9593.0
10	10362.0
11	11122.0
12	11081.0
13	11153.0
14	11786.0
15	12818.0
16	12824.0
17	13307.0
18	13096.0
19	12464.0
20	12828.0
21	13439.0
22	14664.0
23	16863.0
24	19704.0
25	23190.0
26	27690.0
27	32775.0
28	38731.0
29	46017.0
30	54532.0
31	66387.0
32	81923.0
33	104836.0
34	143791.0
35	207770.0
36	345384.0
37	625941.0
38	674185.0
39	124486.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.02476894140212	17.67158443558218	11.446593975085856	25.857052647929844
2	16.808858734713507	19.63280671605899	37.74604969494566	25.812284854281835
3	19.416521215199722	22.886518135051013	29.547170856389414	28.149789793359847
4	13.35017953976218	14.935303542431635	36.209887250650475	35.504629667155704
5	14.147987068009126	36.86090207154337	34.815700893169236	14.175409967278267
6	34.53108344657701	34.8895477611507	16.5477523747417	14.031616417530593
7	29.26924193428612	31.395390441985594	21.006501178043166	18.328866445685122
8	26.9732971241369	34.293304618022795	20.036254204761995	18.697144053078315
9	26.76094178814458	14.59820902696045	19.10618760224886	39.53466158264611
10-11	25.835562416783837	24.733147108031098	28.28676941294136	21.144521062243708
12-13	26.05039449502881	22.324398062565574	27.78951803145428	23.83568941095134
14-15	23.22573108181978	24.006960770708847	24.748315264569047	28.01899288290232
16-17	23.71156451469698	27.016861801556242	25.740723173355473	23.530850510391307
18-19	23.670523777321012	26.480398201121403	26.78819971555616	23.06087830600142
20-21	24.118549075175093	25.818906987519064	26.681289227100063	23.381254710205777
22-23	23.92973148265849	25.757548900792532	26.394940169762798	23.917779446786177
24-25	23.728114287990802	25.795657615446192	26.562507912938933	23.913720183624072
26-27	23.648898751357265	25.98229110421594	26.509854769770687	23.858955374656116
28-29	23.783631614363056	26.24495009971955	26.309573296189765	23.66184498972763
30-31	23.94399452501926	25.97113821035301	26.189040418488357	23.89582684613937
32-33	23.64604040643723	26.072785997367582	26.175682994216537	24.105490601978655
34-35	24.097600622120936	25.989429479459748	26.216683182753425	23.69628671566589
36-37	24.104830331884077	25.81710431192122	26.23703045190155	23.841034904293156
38-39	23.88639979289941	25.958553218792353	26.285793071884594	23.86925391642364
40-41	24.062265642350063	25.78148467406441	26.420135638023197	23.736114045562335
42-43	23.853730298813204	25.768933323118826	26.25282019306432	24.12451618500365
44-45	23.774430616567113	25.83799489523257	26.435878565572864	23.951695922627454
46-47	23.941954553339585	25.79641892570476	26.223975566646118	24.037650954309534
48-49	23.65778101746948	25.93992062755331	26.286682618259317	24.1156157367179
50-51	23.72380902142348	25.99519293846177	26.351783170956367	23.929214869158386
52-53	23.928547334170073	25.786031232769336	26.33553575888149	23.949885674179104
54-55	23.438151478231052	25.677537360295172	26.630071448933236	24.25423971254054
56-57	23.49451401554854	25.85870100585822	26.49959343978014	24.1471915388131
58-59	23.57825503056096	25.657526505160682	26.541313690085854	24.222904774192504
60-61	23.463461174887772	25.71465919271743	26.64679468296199	24.175084949432808
62-63	23.574907145631258	25.65981256833992	26.672566422554965	24.092713863473854
64-65	23.398799054907546	25.55621572103879	26.57129060172712	24.47369462232654
66-67	23.35288530176884	25.67844349829419	26.48349968737816	24.48517151255881
68-69	23.586085470484296	25.539112529707424	26.536413015085802	24.338388984722474
70-71	23.493731283534107	25.39378751111957	26.487334592677968	24.62514661266835
72-73	23.498257441484846	25.590426638988166	26.39364526198737	24.517670657539615
74-75	23.51750211213586	25.69511967699073	26.36520397137335	24.42217423950006
76-77	23.549628612752187	25.474992751460906	26.462535407951158	24.51284322783574
78-79	23.465453933646323	25.565568663059917	26.475236793224116	24.493740610069644
80-81	23.708793076448785	25.57187517016823	26.300795533297507	24.418536220085482
82-83	23.633258491417646	25.640004917683758	26.313632891525334	24.413103699373266
84-85	23.602291007149294	25.63173739218101	26.365250634497915	24.40072096617178
86-87	23.674224678304455	25.68060835730201	26.27711634417339	24.368050620220146
88-89	23.638249148364014	25.479775093348913	26.26030541696805	24.621670341319028
90-91	23.708361063731008	25.478314476662916	26.273837923683125	24.53948653592295
92-93	23.854805878146774	25.358251574020397	26.382388019112163	24.40455452872067
94-95	23.808877091178466	25.200608527331724	26.471987531785746	24.518526849704067
96-97	23.88480206433315	25.063174196376863	26.40096180388294	24.65106193540705
98-99	23.860205580824093	25.092939600802406	26.317978335149476	24.728876483224024
100-101	24.136311948463465	24.953143761418065	26.33050828680128	24.58003600331719
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	37.0
2	71.5
3	118.5
4	115.0
5	130.5
6	209.0
7	346.0
8	524.5
9	594.5
10	514.0
11	476.5
12	529.0
13	587.0
14	663.5
15	796.5
16	911.5
17	998.0
18	1071.5
19	1153.5
20	1306.0
21	1452.5
22	1637.0
23	1863.5
24	2117.5
25	2566.0
26	3226.5
27	4236.5
28	5783.5
29	8136.5
30	11817.5
31	17023.0
32	24251.5
33	29824.5
34	34050.5
35	40545.0
36	49426.0
37	58351.0
38	66784.5
39	74903.0
40	81857.5
41	89020.5
42	96197.0
43	103737.5
44	110701.0
45	117927.5
46	123244.5
47	126581.0
48	129908.0
49	131256.5
50	131465.0
51	130870.5
52	129585.5
53	127462.5
54	123652.0
55	116777.5
56	107001.5
57	95599.0
58	85795.0
59	75154.0
60	61712.0
61	50553.0
62	41560.5
63	32017.5
64	23104.0
65	16932.0
66	12854.0
67	10154.5
68	7870.0
69	5924.0
70	4488.5
71	3250.0
72	2279.5
73	1777.0
74	1453.5
75	1074.0
76	793.5
77	553.5
78	361.5
79	246.0
80	158.0
81	132.5
82	124.0
83	95.0
84	69.5
85	56.5
86	53.5
87	49.0
88	40.5
89	36.0
90	36.5
91	32.5
92	24.5
93	20.0
94	15.0
95	12.0
96	12.0
97	11.5
98	6.5
99	4.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05234699136426519
2	0.07212477146707591
3	0.06834471793206771
4	0.06807471410813856
5	0.06385590435924549
6	0.009551385271493906
7	0.012858932114626073
8	0.017077741863519142
9	0.005872583170459151
10-11	0.012842056875630501
12-13	0.02203906212821739
14-15	0.039825564029550564
16-17	0.02639287378907504
18-19	0.010040767202365504
20-21	0.020368413467655735
22-23	0.036534892425413974
24-25	0.03375047799114455
26-27	0.02107717350546977
28-29	0.0122345482717899
30-31	0.011930793969869598
32-33	0.008420744258790566
34-35	0.005163823132645116
36-37	6.412590818317464E-4
38-39	0.003695677340030328
40-41	0.0011812667296900593
42-43	0.21402865618084319
44-45	0.185273248932388
46-47	0.19075770160594901
48-49	0.19742342100920002
50-51	0.1891714291403652
52-53	0.19583714854361625
54-55	0.1925127264614885
56-57	0.1955671447196871
58-59	0.20398788897847764
60-61	0.20797044538143272
62-63	0.19360961699620072
64-65	0.19406524844908116
66-67	0.19150021212175417
68-69	0.18689327187596294
70-71	0.17881003239708382
72-73	0.19006581680713053
74-75	0.1892220548573519
76-77	0.1832144697749282
78-79	0.20127097550019052
80-81	0.21222300560831694
82-83	0.21075485981570213
84-85	0.20437601947537581
86-87	0.22383317003727066
88-89	0.21618868677227643
90-91	0.22371504336430162
92-93	0.21866934690462553
94-95	0.22287128141452303
96-97	0.22980700464170323
98-99	0.22158876325085955
100-101	0.2296551274907431
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2962921.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.83194007506836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.53957157536786	50.60457534895192
2	10.973753476792373	13.790044417047827
3	3.496241742979378	6.590269550484986
4	1.554747241882335	3.907511421353147
5	0.884067871745426	2.7773849769900916
6	0.5514376929746484	2.078874004807023
7	0.36390185986692686	1.6005261896655272
8	0.28309444754925406	1.422989869119945
9	0.21392703261859536	1.20973054445359
>10	1.0796828011971817	11.785358927669941
>50	0.045601819681252896	1.9349551697502465
>100	0.012359172558972118	1.3228552768839952
>500	0.001021734364586521	0.44573374249255143
>1k	5.91530421268967E-4	0.529190560329268
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.375047799114455E-5
2	0.0	0.0	0.0	0.0	3.375047799114455E-5
3	0.0	3.375047799114455E-5	0.0	0.0	3.375047799114455E-5
4	0.0	3.375047799114455E-5	0.0	0.0	6.75009559822891E-5
5	0.0	3.375047799114455E-5	0.0	0.0	6.75009559822891E-5
6	0.0	3.375047799114455E-5	0.0	0.0	6.75009559822891E-5
7	0.0	3.375047799114455E-5	0.0	0.0	6.75009559822891E-5
8	0.0	3.375047799114455E-5	0.0	0.0	1.0125143397343364E-4
9	0.0	3.375047799114455E-5	0.0	3.375047799114455E-5	2.3625334593801184E-4
10-11	0.0	3.375047799114455E-5	0.0	3.375047799114455E-5	2.3625334593801184E-4
12-13	0.0	3.375047799114455E-5	0.0	5.062571698671682E-5	3.037543019203009E-4
14-15	0.0	3.375047799114455E-5	0.0	6.75009559822891E-5	3.037543019203009E-4
16-17	0.0	3.375047799114455E-5	0.0	6.75009559822891E-5	3.037543019203009E-4
18-19	0.0	3.375047799114455E-5	0.0	1.0125143397343364E-4	3.037543019203009E-4
20-21	0.0	3.375047799114455E-5	0.0	1.0125143397343364E-4	3.206295409158732E-4
22-23	0.0	3.375047799114455E-5	0.0	1.350019119645782E-4	4.556314528804514E-4
24-25	0.0	3.375047799114455E-5	0.0	3.206295409158732E-4	5.568828868538851E-4
26-27	0.0	3.375047799114455E-5	0.0	7.256352768096079E-4	6.412590818317464E-4
28-29	0.0	3.375047799114455E-5	0.0	0.006901972749189061	6.75009559822891E-4
30-31	0.0	3.375047799114455E-5	0.0	0.031506071204733435	6.75009559822891E-4
32-33	0.0	3.375047799114455E-5	0.0	0.08073114335481776	6.75009559822891E-4
34-35	1.6875238995572275E-5	6.75009559822891E-5	0.0	0.14860335459500945	6.75009559822891E-4
36-37	3.375047799114455E-5	6.75009559822891E-5	0.0	0.2579211528083266	6.75009559822891E-4
38-39	3.375047799114455E-5	6.75009559822891E-5	0.0	0.4357524213436673	7.087600378140356E-4
40-41	3.375047799114455E-5	6.75009559822891E-5	0.0	0.6300032974216998	7.087600378140356E-4
42-43	3.375047799114455E-5	6.75009559822891E-5	0.0	0.8352905798028365	7.087600378140356E-4
44-45	3.375047799114455E-5	6.75009559822891E-5	0.0	1.0818715720061385	7.256352768096079E-4
46-47	3.375047799114455E-5	6.75009559822891E-5	0.0	1.3759563619819766	7.425105158051801E-4
48-49	5.062571698671682E-5	6.75009559822891E-5	0.0	1.6705980348446685	8.100114717874691E-4
50-51	6.75009559822891E-5	6.75009559822891E-5	0.0	1.9800392923064773	8.100114717874691E-4
52-53	6.75009559822891E-5	6.75009559822891E-5	0.0	2.2972431597062495	8.775124277697583E-4
54-55	6.75009559822891E-5	6.75009559822891E-5	0.0	2.6533782034688067	9.28138144756475E-4
56-57	6.75009559822891E-5	6.75009559822891E-5	0.0	3.06395276823108	9.450133837520474E-4
58-59	6.75009559822891E-5	1.0125143397343364E-4	0.0	3.489073789007537	9.450133837520474E-4
60-61	6.75009559822891E-5	1.0125143397343364E-4	0.0	3.9301925363517958	9.618886227476197E-4
62-63	6.75009559822891E-5	1.0125143397343364E-4	0.0	4.380744542294581	9.78763861743192E-4
64-65	1.0125143397343364E-4	1.1812667296900592E-4	0.0	4.8670551796689825	0.0010125143397343365
66-67	1.0125143397343364E-4	1.350019119645782E-4	0.0	5.4265368533281855	0.001046264817725481
68-69	1.350019119645782E-4	2.0250286794686728E-4	0.0	6.026957856790647	0.0010800152957166256
70-71	1.350019119645782E-4	2.1937810694243955E-4	0.0	6.635900855945872	0.0011981419686856315
72-73	1.350019119645782E-4	2.3625334593801184E-4	0.0	7.257348407196817	0.0012487676856723483
74-75	1.350019119645782E-4	2.3625334593801184E-4	0.0	7.906302598010544	0.001282518163663493
76-77	1.350019119645782E-4	2.3625334593801184E-4	0.0	8.635161045468307	0.001282518163663493
78-79	1.350019119645782E-4	2.3625334593801184E-4	3.375047799114455E-5	9.423268457039523	0.001282518163663493
80-81	1.350019119645782E-4	2.3625334593801184E-4	3.375047799114455E-5	10.222817280649737	0.0013331438806502096
82-83	1.350019119645782E-4	2.5312858493358413E-4	3.375047799114455E-5	11.039798226142377	0.0014512705536192157
84-85	1.350019119645782E-4	2.700038239291564E-4	3.375047799114455E-5	11.878632606134284	0.0014512705536192157
86-87	1.350019119645782E-4	2.700038239291564E-4	3.375047799114455E-5	12.77597343972384	0.001468145792614788
88-89	1.350019119645782E-4	2.700038239291564E-4	3.375047799114455E-5	13.741996496025376	0.0015187715096015048
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1905	0.0	43.350986	1
GTATCAA	3140	0.0	39.299625	1
TCAACGC	3510	0.0	33.674072	4
CAACGCA	3595	0.0	33.00825	5
ATCAACG	3750	0.0	31.39023	3
AACGCAG	3995	0.0	29.569	6
TATCAAC	3965	0.0	29.328487	2
GTACATG	6130	0.0	27.098938	1
TACATGG	6415	0.0	25.08244	2
ACATGGG	6685	0.0	23.643778	3
ACGCAGA	4830	0.0	23.47932	7
GAGTACA	4740	0.0	22.329103	1
CGCAGAG	5055	0.0	21.872519	8
CATGGGG	5590	0.0	20.634687	4
GTATAGA	795	0.0	19.104143	1
AGTACAT	5065	0.0	18.929478	2
TGGACCG	590	0.0	18.503643	5
AGAGTAC	4755	0.0	17.415447	10-11
GTATAGC	775	0.0	17.147509	1
CTATACT	950	0.0	16.988646	4
>>END_MODULE