FastQCFastQC Report
Mon 15 Aug 2016
SRR2921669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962921
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18200.044.914251
GTATCAA31300.040.085441
TCAACGC36600.033.5820774
CAACGCA38400.031.9069525
ATCAACG39350.030.9939733
TATCAAC40100.030.295942
AACGCAG41750.029.4595036
GTACATG58550.025.568821
ACGCAGA49000.024.4218927
TACATGG63350.022.8476812
CGCAGAG52650.022.3680528
GAGTACA44650.021.3944741
ACATGGG65050.021.375153
TATACTG10750.020.3210545
CATGGGG55000.019.5862854
GCAGAGT62450.018.173889
AGTACAT50300.017.359562
AGAGTAC49700.017.05548310-11
GTACTAA6200.016.097361
ATACCGT3552.992965E-816.052226
Produced by FastQC (version 0.11.5)